Restriction map for
pFN21AB9571
(Circular) (Six-base) MAPSORT of: pFN21AB9571.seq Check: 63 from: 1 to: 5659
pFN21AB9571 5659 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2269 2269
Size: 5659
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3469 278
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2015 3002 1937
Size: 78 987 4594
Fragments arranged by size:
4594 987 78
Acc65I G'GTAC_C
Cuts at: 2980 2980
Size: 5659
AclI AA'CG_TT
Cuts at: 1563 3786 4159 1563
Size: 2223 373 3063
Fragments arranged by size:
3063 2223 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2427 3722 5106 5374 1632
Size: 795 1295 1384 268 1917
Fragments arranged by size:
1917 1384 1295 795 268
AflII C'TTAA_G
Cuts at: 820 1017 2744 820
Size: 197 1727 3735
Fragments arranged by size:
3735 1727 197
AflIII A'CryG_T
Cuts at: 1203 4558 5535 1203
Size: 3355 977 1327
Fragments arranged by size:
3355 1327 977
AgeI A'CCGG_T
Cuts at: 4490 4490
Size: 5659
AhdI GACnn_n'nnGTC
Cuts at: 4388 4388
Size: 5659
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5659
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2388 2850 1184
Size: 1204 462 3993
Fragments arranged by size:
3993 1204 462
AlwNI CAG_nnn'CTG
Cuts at: 2474 3402 4974 2474
Size: 928 1572 3159
Fragments arranged by size:
3159 1572 928
ApaLI G'TGCA_C
Cuts at: 3716 4872 3716
Size: 1156 4503
ApoI r'AATT_y
Cuts at: 1493 2787 2968 3194 3346 5455 1493
Size: 1294 181 226 152 2109 1697
Fragments arranged by size:
2109 1697 1294 226 181 152
AseI AT'TA_AT
Cuts at: 160 1032 2096 4213 160
Size: 872 1064 2117 1606
Fragments arranged by size:
2117 1606 1064 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
AvaI C'yCGr_G
Cuts at: 1870 1943 2760 2984 1870
Size: 73 817 224 4545
Fragments arranged by size:
4545 817 224 73
BamHI G'GATC_C
Cuts at: 2908 2989 3356 2908
Size: 81 367 5211
Fragments arranged by size:
5211 367 81
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2447 2826 2980 4435 618
Size: 325 217 627 660 379 154 1455 1842
Fragments arranged by size:
1842 1455 660 627 379 325 217 154
BanII G_rGCy'C
Cuts at: 1391 2978 1391
Size: 1587 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2804 2838 3850 3884 1903
Size: 34 867 34 1012 34 3678
Fragments arranged by size:
3678 1012 867 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3551 4761 3551
Size: 1210 4449
BclI T'GATC_A
Cuts at: 1548 2487 1548
Size: 939 4720
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2295 2510 4270
Size: 107 122 71 631 605 623 215 1760
Cuts at: 4270 136
Size: 1525
Fragments arranged by size:
1760 1525 631 623 605 215 122 107
71
BglII A'GATC_T
Cuts at: 5654 5654
Size: 5659
BlpI GC'TnA_GC
Cuts at: 3076 3076
Size: 5659
Bme1580I G_kGCm'C
Cuts at: 1790 3720 4876 1790
Size: 1930 1156 2573
Fragments arranged by size:
2573 1930 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5659
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 4348 442
Size: 416 53 83 399 24 1973 958 1753
Fragments arranged by size:
1973 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5659
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2441 2703 4319 1239
Size: 1202 262 1616 2579
Fragments arranged by size:
2579 1616 1202 262
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3784 4649 4947 3784
Size: 865 298 4496
Fragments arranged by size:
4496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4322 882
Size: 617 2823 2219
Fragments arranged by size:
2823 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2716 3450 493
Size: 711 1512 734 2702
Fragments arranged by size:
2702 1512 734 711
BsaBI GATnn'nnATC
Cuts at: 2255 2315 2492 5653 2255
Size: 60 177 3161 2261
Fragments arranged by size:
3161 2261 177 60
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3466 3848 4483 275
Size: 53 83 186 936 1933 382 635 1451
Fragments arranged by size:
1933 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 4490 4764 4911 5322 4092
Size: 398 274 147 411 4429
Fragments arranged by size:
4429 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2610 2640 1178
Size: 30 165 30 1207 30 4197
Fragments arranged by size:
4197 1207 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2764 1186
Size: 1578 4081
BseYI C'CCAG_C
Cuts at: 2304 2497 4862 5358 2304
Size: 193 2365 496 2605
Fragments arranged by size:
2605 2365 496 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2297 2303 1163
Size: 573 387 174 6 4519
Fragments arranged by size:
4519 573 387 174 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3109 3870 4898 1540
Size: 275 186 1108 761 1028 2301
Fragments arranged by size:
2301 1108 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2521 3720 3805 4876 2521
Size: 1199 85 1071 3304
Fragments arranged by size:
3304 1199 1071 85
BsmI GAATG_Cn'
Cuts at: 2725 3170 3263 2725
Size: 445 93 5121
Fragments arranged by size:
5121 445 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2521 2978 3720 3805 4876 1391
Size: 399 731 457 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 731 457 399 85
BspEI T'CCGG_A
Cuts at: 5322 5322
Size: 5659
BspHI T'CATG_A
Cuts at: 2628 3548 5278 2628
Size: 920 1730 3009
Fragments arranged by size:
3009 1730 920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2068 2095 2269 2820 3014 844
Size: 1224 27 174 551 194 3489
Fragments arranged by size:
3489 1224 551 194 174 27
BsrBI CCG'CTC
Cuts at: 1394 1823 3546 1394
Size: 429 1723 3507
Fragments arranged by size:
3507 1723 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2608 2940 4154 4328 65
Size: 1183 1360 332 1214 174 1396
Fragments arranged by size:
1396 1360 1214 1183 332 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4303 4490 1700
Size: 110 2493 187 2869
Fragments arranged by size:
2869 2493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5659
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5659
BssSI C'ACGA_G
Cuts at: 3719 4731 3719
Size: 1012 4647
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2402 794
Size: 1608 4051
BstBI TT'CG_AA
Cuts at: 4463 4463
Size: 5659
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5659
BstYI r'GATC_y
Cuts at: 1972 2250 2256 2908 2989 3356 3741 3758 5210
Size: 278 6 652 81 367 385 17 1452
Cuts at: 5210 5296 5308 5654 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 652 385 367 346 278 86
81 17 12 6
BstZ17I GTA'TAC
Cuts at: 2016 2016
Size: 5659
BtgI C'CryG_G
Cuts at: 513 1065 2004 2838 513
Size: 552 939 834 3334
Fragments arranged by size:
3334 939 834 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4920
Fragments arranged by size:
4920 683 56
BtsI GCAGTG_nn'
Cuts at: 2340 2566 3175 3969 3989 2340
Size: 226 609 794 20 4010
Fragments arranged by size:
4010 794 609 226 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5659
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5624
DraI TTT'AAA
Cuts at: 2963 2963
Size: 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4666 809
Size: 507 3350 1802
Fragments arranged by size:
3350 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2137 2347 3106
Size: 54 1425 50 275 18 307 210 759
Cuts at: 3106 3995 5624 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 759 307 275 210 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3106 1537
Size: 275 1294 4090
Fragments arranged by size:
4090 1294 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2697 3589 1353
Size: 1344 892 3423
Fragments arranged by size:
3423 1344 892
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4246 4620 4766 387
Size: 1064 138 2657 374 146 1280
Fragments arranged by size:
2657 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2427 2441 2703 3722 4319 5106 5374
Size: 393 795 14 262 1019 597 787 268
Cuts at: 5374 1239
Size: 1524
Fragments arranged by size:
1524 1019 795 787 597 393 268 262
14
EcoNI CCTnn'n_nnAGG
Cuts at: 2478 2478
Size: 5659
EcoRI G'AATT_C
Cuts at: 2968 3346 2968
Size: 378 5281
FspI TGC'GCA
Cuts at: 4165 4165
Size: 5659
HaeII r_GCGC'y
Cuts at: 1678 4806 1678
Size: 3128 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2610 2642 4300 4332 4374
Size: 32 135 32 1038 32 1658 32 42
Cuts at: 4374 4406 1373
Size: 32 2626
Fragments arranged by size:
2626 1658 1038 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3003 3249 669
Size: 988 281 1065 246 3079
Fragments arranged by size:
3079 1065 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5659
HpaI GTT'AAC
Cuts at: 3249 3249
Size: 5659
KpnI G_GTAC'C
Cuts at: 2984 2984
Size: 5659
MfeI C'AATT_G
Cuts at: 3258 3258
Size: 5659
MluI A'CGCG_T
Cuts at: 5535 5535
Size: 5659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4773 4957 1305
Size: 3468 184 2007
Fragments arranged by size:
3468 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2139 2349 10
Size: 54 1425 343 307 210 3320
Fragments arranged by size:
3320 1425 343 307 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2832 2951 3617 3976 4135 518
Size: 648 1666 119 666 359 159 2042
Fragments arranged by size:
2042 1666 666 648 359 159 119
MspA1I CmG'CkG
Cuts at: 1606 3075 3752 4900 5145 1606
Size: 1469 677 1148 245 2120
Fragments arranged by size:
2120 1469 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2838 513
Size: 552 939 834 3334
Fragments arranged by size:
3334 939 834 552
NdeI CA'TA_TG
Cuts at: 387 2901 387
Size: 2514 3145
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5659
NotI GC'GGCC_GC
Cuts at: 3106 3106
Size: 5659
NspI r_CATG'y
Cuts at: 1132 1229 3017 4562 1132
Size: 97 1788 1545 2229
Fragments arranged by size:
2229 1788 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5659
PciI A'CATG_T
Cuts at: 4558 4558
Size: 5659
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5659
PmeI GTTT'AAAC
Cuts at: 2963 2963
Size: 5659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3728 3760 5241 5273 3728
Size: 32 1481 32 4114
Fragments arranged by size:
4114 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2336 2420 1613
Size: 723 84 4852
Fragments arranged by size:
4852 723 84
PsiI TTA'TAA
Cuts at: 3229 3229
Size: 5659
PstI C_TGCA'G
Cuts at: 830 3011 830
Size: 2181 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5659
SalI G'TCGA_C
Cuts at: 1936 3001 1936
Size: 1065 4594
SapI GCTCTTCn'nnn_
Cuts at: 2697 2697
Size: 5659
SbfI CC_TGCA'GG
Cuts at: 3011 3011
Size: 5659
ScaI AGT'ACT
Cuts at: 3907 5619 3907
Size: 1712 3947
SfcI C'TryA_G
Cuts at: 826 1045 3007 4142 4823 5014 826
Size: 219 1962 1135 681 191 1471
Fragments arranged by size:
1962 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2295 2295
Size: 5659
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5659
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5659
SmaI CCC'GGG
Cuts at: 1872 2986 1872
Size: 1114 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2744 3763 4664 4926 820
Size: 197 926 801 1019 901 262 1553
Fragments arranged by size:
1553 1019 926 901 801 262 197
SnaBI TAC'GTA
Cuts at: 493 3450 493
Size: 2957 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5659
SphI G_CATG'C
Cuts at: 1132 3017 1132
Size: 1885 3774
SspI AAT'ATT
Cuts at: 5 52 3583 5
Size: 47 3531 2081
Fragments arranged by size:
3531 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5659
StyI C'CwwG_G
Cuts at: 513 1065 2004 2083 2838 3098 513
Size: 552 939 79 755 260 3074
Fragments arranged by size:
3074 939 755 552 260 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3686 3703 3856 4041 4486 325
Size: 1255 2106 17 153 185 445 1498
Fragments arranged by size:
2106 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3905 5617 96
Size: 274 80 33 51 1443 1928 1712 138
Fragments arranged by size:
1928 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2259 2291 2376 2408 2839 2871 2931 2963 5619
Size: 32 85 32 431 32 60 32 2656
Cuts at: 5619 5651 2259
Size: 32 2267
Fragments arranged by size:
2656 2267 431 85 60 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2175 1316
Size: 859 4800
XbaI T'CTAG_A
Cuts at: 2995 2995
Size: 5659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2162 2261 2162
Size: 99 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5659
XmaI C'CCGG_G
Cuts at: 1870 2984 1870
Size: 1114 4545
XmnI GAAnn'nnTTC
Cuts at: 3788 3788
Size: 5659
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3467 276
Size: 53 83 186 936 1933 2468
Fragments arranged by size:
2468 1933 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII
EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfoI SrfI SwaI