Restriction map for
pFN21AB9569
(Circular) (Six-base) MAPSORT of: pFN21AB9569.seq Check: 8872 from: 1 to: 6928
pFN21AB9569 6928 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4738 278
Size: 53 83 186 936 3202 2468
Fragments arranged by size:
3202 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4271 1937
Size: 2334 4594
Acc65I G'GTAC_C
Cuts at: 4249 4249
Size: 6928
AclI AA'CG_TT
Cuts at: 1563 5055 5428 1563
Size: 3492 373 3063
Fragments arranged by size:
3492 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 2944 3474 4991 6375 6643 1632
Size: 624 688 530 1517 1384 268 1917
Fragments arranged by size:
1917 1517 1384 688 624 530 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6731
AflIII A'CryG_T
Cuts at: 1203 3012 5827 6804 1203
Size: 1809 2815 977 1327
Fragments arranged by size:
2815 1809 1327 977
AgeI A'CCGG_T
Cuts at: 5759 5759
Size: 6928
AhdI GACnn_n'nnGTC
Cuts at: 5657 5657
Size: 6928
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2454 2457 1184
Size: 1270 3 5655
Fragments arranged by size:
5655 1270 3
AlwNI CAG_nnn'CTG
Cuts at: 2395 3719 3722 3782 4094 4671 6243 2395
Size: 1324 3 60 312 577 1572 3080
Fragments arranged by size:
3080 1572 1324 577 312 60 3
ApaLI G'TGCA_C
Cuts at: 4985 6141 4985
Size: 1156 5772
ApoI r'AATT_y
Cuts at: 1493 3103 4237 4463 4615 6724 1493
Size: 1610 1134 226 152 2109 1697
Fragments arranged by size:
2109 1697 1610 1134 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5482 160
Size: 872 4450 1606
Fragments arranged by size:
4450 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
AvaI C'yCGr_G
Cuts at: 1870 1943 4253 1870
Size: 73 2310 4545
Fragments arranged by size:
4545 2310 73
BamHI G'GATC_C
Cuts at: 4258 4625 4258
Size: 367 6561
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3808 3833 4249 5704 618
Size: 325 217 627 2021 25 416 1455 1842
Fragments arranged by size:
2021 1842 1455 627 416 325 217 25
BanII G_rGCy'C
Cuts at: 1391 2844 2931 4000 4247 1391
Size: 1453 87 1069 247 4072
Fragments arranged by size:
4072 1453 1069 247 87
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 5841
Fragments arranged by size:
5841 971 116
BbvCI CC'TCA_GC
Cuts at: 2685 2999 3547 3589 2685
Size: 314 548 42 6024
Fragments arranged by size:
6024 548 314 42
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5119 5153 1903
Size: 34 3182 34 3678
Fragments arranged by size:
3678 3182 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4820 6030 4820
Size: 1210 5718
BclI T'GATC_A
Cuts at: 1548 3238 1548
Size: 1690 5238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4190 4220 5539
Size: 107 122 71 631 605 2518 30 1319
Cuts at: 5539 136
Size: 1525
Fragments arranged by size:
2518 1525 1319 631 605 122 107 71
30
BglII A'GATC_T
Cuts at: 2022 6923 2022
Size: 4901 2027
BlpI GC'TnA_GC
Cuts at: 4345 4345
Size: 6928
Bme1580I G_kGCm'C
Cuts at: 1790 3100 4989 6145 1790
Size: 1310 1889 1156 2573
Fragments arranged by size:
2573 1889 1310 1156
BmgBI CAC'GTC
Cuts at: 1318 3558 1318
Size: 2240 4688
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2337 2838 3975
Size: 416 53 83 399 24 920 501 1137
Cuts at: 3975 4659 5617 442
Size: 684 958 1753
Fragments arranged by size:
1753 1137 958 920 684 501 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2312 1056
Size: 1256 5672
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3082 3888 3082
Size: 806 6122
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2334 2351 2466 3070 3093 3522 3849 5588
Size: 1095 17 115 604 23 429 327 1739
Cuts at: 5588 1239
Size: 2579
Fragments arranged by size:
2579 1739 1095 604 429 327 115 23
17
Bpu10I CC'TnA_GC
Cuts at: 2685 2999 3547 3589 3863 2685
Size: 314 548 42 274 5750
Fragments arranged by size:
5750 548 314 274 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2873 2882 3145 5053 5918 6216 2873
Size: 9 263 1908 865 298 3585
Fragments arranged by size:
3585 1908 865 298 263 9
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2654 3152 3617 4081 5591 882
Size: 617 1155 498 465 464 1510 2219
Fragments arranged by size:
2219 1510 1155 617 498 465 464
BsaAI yAC'GTr
Cuts at: 493 1204 4719 493
Size: 711 3515 2702
Fragments arranged by size:
3515 2702 711
BsaBI GATnn'nnATC
Cuts at: 2021 6922 2021
Size: 4901 2027
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2342 2630 4735 5117
Size: 53 83 186 936 809 288 2105 382
Cuts at: 5117 5752 275
Size: 635 1451
Fragments arranged by size:
2105 1451 936 809 635 382 288 186
83 53
BsaWI w'CCGG_w
Cuts at: 5361 5759 6033 6180 6591 5361
Size: 398 274 147 411 5698
Fragments arranged by size:
5698 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3485 3515 3520 3550 3634
Size: 30 165 30 2082 30 5 30 84
Cuts at: 3634 3664 4118 4148 1178
Size: 30 454 30 3958
Fragments arranged by size:
3958 2082 454 165 84 30 30 30
30 30 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2121 2852 2870 4008 1186
Size: 935 731 18 1138 4106
Fragments arranged by size:
4106 1138 935 731 18
BseYI C'CCAG_C
Cuts at: 2262 2298 2349 2739 2828 3362 3880 6131 6627
Size: 36 51 390 89 534 518 2251 496
Cuts at: 6627 2262
Size: 2563
Fragments arranged by size:
2563 2251 534 518 496 390 89 51
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2297 2429 3432 3723 4092 1163
Size: 573 561 132 1003 291 369 3999
Fragments arranged by size:
3999 1003 573 561 369 291 132
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4378 5139 6167 1540
Size: 275 186 2377 761 1028 2301
Fragments arranged by size:
2377 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3874 4989 5074 6145 3874
Size: 1115 85 1071 4657
Fragments arranged by size:
4657 1115 1071 85
BsmI GAATG_Cn'
Cuts at: 4439 4532 4439
Size: 93 6835
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2844 2931 3100 3874 4000 4247 4989
Size: 399 1054 87 169 774 126 247 742
Cuts at: 4989 5074 6145 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1054 774 742 399 247 169
126 87 85
BspEI T'CCGG_A
Cuts at: 6591 6591
Size: 6928
BspHI T'CATG_A
Cuts at: 4817 6547 4817
Size: 1730 5198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2571 4283 844
Size: 1727 1712 3489
Fragments arranged by size:
3489 1727 1712
BsrBI CCG'CTC
Cuts at: 1394 1823 3867 4815 1394
Size: 429 2044 948 3507
Fragments arranged by size:
3507 2044 948 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5423 5597 65
Size: 1183 4175 174 1396
Fragments arranged by size:
4175 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5572 5759 1700
Size: 110 3762 187 2869
Fragments arranged by size:
3762 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2206 96
Size: 2110 4818
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6928
BssSI C'ACGA_G
Cuts at: 4988 6000 4988
Size: 1012 5916
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2095 3614 3719 3722 3782 4094 794
Size: 1301 1519 105 3 60 312 3628
Fragments arranged by size:
3628 1519 1301 312 105 60 3
BstBI TT'CG_AA
Cuts at: 5732 5732
Size: 6928
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6928
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2168 2074
Size: 94 6834
BstYI r'GATC_y
Cuts at: 1972 2022 2487 2521 4258 4625 5010 5027 6479
Size: 50 465 34 1737 367 385 17 1452
Cuts at: 6479 6565 6577 6923 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1737 1452 465 385 367 346 86
50 34 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3771 513
Size: 552 2706 3670
Fragments arranged by size:
3670 2706 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6189
Fragments arranged by size:
6189 683 56
BtsI GCAGTG_nn'
Cuts at: 2412 3328 3415 4444 5238 5258 2412
Size: 916 87 1029 794 20 4082
Fragments arranged by size:
4082 1029 916 794 87 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6893
DraI TTT'AAA
Cuts at: 2202 4232 2202
Size: 2030 4898
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5935 809
Size: 507 4619 1802
Fragments arranged by size:
4619 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4375 5264 6893
Size: 54 1425 50 275 18 2545 889 1629
Cuts at: 6893 8
Size: 43
Fragments arranged by size:
2545 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4375 1537
Size: 275 2563 4090
Fragments arranged by size:
4090 2563 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3179 3213 3945 4172 4858 1353
Size: 1826 34 732 227 686 3423
Fragments arranged by size:
3423 1826 732 686 227 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2410 2449 5515 5889 6035 387
Size: 1064 138 821 39 3066 374 146 1280
Fragments arranged by size:
3066 1280 1064 821 374 146 138 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2334 2351 2466 2944 3070 3093
Size: 393 624 78 17 115 478 126 23
Cuts at: 3093 3474 3522 3849 4991 5588 6375 6643 1239
Size: 381 48 327 1142 597 787 268 1524
Fragments arranged by size:
1524 1142 787 624 597 478 393 381
327 268 126 115 78 48 23 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2103 2103
Size: 6928
EcoO109I rG'GnC_Cy
Cuts at: 3858 3858
Size: 6928
EcoRI G'AATT_C
Cuts at: 3103 4237 4615 3103
Size: 1134 378 5416
Fragments arranged by size:
5416 1134 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2274 3208 2274
Size: 934 5994
FspI TGC'GCA
Cuts at: 5434 5434
Size: 6928
HaeII r_GCGC'y
Cuts at: 1678 3536 6075 1678
Size: 1858 2539 2531
Fragments arranged by size:
2539 2531 1858
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2097 2129 2439 2471 3223
Size: 32 135 32 525 32 310 32 752
Cuts at: 3223 3255 3485 3517 4003 4035 5569 5601 5643
Size: 32 230 32 486 32 1534 32 42
Cuts at: 5643 5675 1373
Size: 32 2626
Fragments arranged by size:
2626 1534 752 525 486 310 230 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 4272 4518 669
Size: 988 281 867 1467 246 3079
Fragments arranged by size:
3079 1467 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6928
HpaI GTT'AAC
Cuts at: 4518 4518
Size: 6928
KpnI G_GTAC'C
Cuts at: 4253 4253
Size: 6928
MfeI C'AATT_G
Cuts at: 4527 4527
Size: 6928
MluI A'CGCG_T
Cuts at: 6804 6804
Size: 6928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2185 2612 3528 6042 6226 1305
Size: 880 427 916 2514 184 2007
Fragments arranged by size:
2514 2007 916 880 427 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5106
Fragments arranged by size:
5106 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2477 3170 4886 5245 5404 518
Size: 648 1311 693 1716 359 159 2042
Fragments arranged by size:
2042 1716 1311 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2392 3719 3779 4188 4344 5021 6169 6414
Size: 786 1327 60 409 156 677 1148 245
Cuts at: 6414 1606
Size: 2120
Fragments arranged by size:
2120 1327 1148 786 677 409 245 156
60
NcoI C'CATG_G
Cuts at: 513 1065 3771 513
Size: 552 2706 3670
Fragments arranged by size:
3670 2706 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6928
NheI G'CTAG_C
Cuts at: 1052 2308 1052
Size: 1256 5672
NotI GC'GGCC_GC
Cuts at: 4375 4375
Size: 6928
NspI r_CATG'y
Cuts at: 1132 1229 3016 4286 5831 1132
Size: 97 1787 1270 1545 2229
Fragments arranged by size:
2229 1787 1545 1270 97
PasI CC'CwG_GG
Cuts at: 1348 2635 1348
Size: 1287 5641
PciI A'CATG_T
Cuts at: 3012 5827 3012
Size: 2815 4113
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3323 3685 1108
Size: 2215 362 4351
Fragments arranged by size:
4351 2215 362
PfoI T'CCnGG_A
Cuts at: 3109 3109
Size: 6928
PmeI GTTT'AAAC
Cuts at: 4232 4232
Size: 6928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4997 5029 6510 6542 4997
Size: 32 1481 32 5383
Fragments arranged by size:
5383 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3858 3858
Size: 6928
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6928
PsiI TTA'TAA
Cuts at: 4498 4498
Size: 6928
PstI C_TGCA'G
Cuts at: 830 2407 3787 4099 4280 830
Size: 1577 1380 312 181 3478
Fragments arranged by size:
3478 1577 1380 312 181
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6928
PvuII CAG'CTG
Cuts at: 2392 3719 3779 2392
Size: 1327 60 5541
Fragments arranged by size:
5541 1327 60
SalI G'TCGA_C
Cuts at: 1936 4270 1936
Size: 2334 4594
SanDI GG'GwC_CC
Cuts at: 3858 3858
Size: 6928
SbfI CC_TGCA'GG
Cuts at: 4280 4280
Size: 6928
ScaI AGT'ACT
Cuts at: 5176 6888 5176
Size: 1712 5216
SexAI A'CCwGG_T
Cuts at: 3972 3972
Size: 6928
SfcI C'TryA_G
Cuts at: 826 1045 2100 2403 2708 3783 4095 4113 4276
Size: 219 1055 303 305 1075 312 18 163
Cuts at: 4276 5411 6092 6283 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 1055 681 312 305 303
219 191 163 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6928
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6928
SmaI CCC'GGG
Cuts at: 1872 4255 1872
Size: 2383 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2852 2861 3124 5032 5933 6195
Size: 197 926 909 9 263 1908 901 262
Cuts at: 6195 820
Size: 1553
Fragments arranged by size:
1908 1553 926 909 901 263 262 197
9
SnaBI TAC'GTA
Cuts at: 493 4719 493
Size: 4226 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6928
SphI G_CATG'C
Cuts at: 1132 4286 1132
Size: 3154 3774
SspI AAT'ATT
Cuts at: 5 52 4852 5
Size: 47 4800 2081
Fragments arranged by size:
4800 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6928
StyI C'CwwG_G
Cuts at: 513 1065 2140 3771 4218 4367 513
Size: 552 1075 1631 447 149 3074
Fragments arranged by size:
3074 1631 1075 552 447 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4955 4972 5125 5310 5755 325
Size: 1255 3375 17 153 185 445 1498
Fragments arranged by size:
3375 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2206 3899 5174
Size: 274 80 33 51 1443 229 1693 1275
Cuts at: 5174 6886 96
Size: 1712 138
Fragments arranged by size:
1712 1693 1443 1275 274 229 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3520 3552 3634 3666 4118 4150 6888 6920 3520
Size: 32 82 32 452 32 2738 32 3528
Fragments arranged by size:
3528 2738 452 82 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6928
XbaI T'CTAG_A
Cuts at: 4264 4264
Size: 6928
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3169 3682 3169
Size: 513 6415
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6928
XmaI C'CCGG_G
Cuts at: 1870 4253 1870
Size: 2383 4545
XmnI GAAnn'nnTTC
Cuts at: 3188 5057 3188
Size: 1869 5059
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4736 276
Size: 53 83 186 936 3202 2468
Fragments arranged by size:
3202 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SapI SfiI SfoI SrfI SwaI