Restriction map for
pFN21AB9568
(Circular) (Six-base) MAPSORT of: pFN21AB9568.seq Check: 2532 from: 1 to: 9040
pFN21AB9568 9040 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3545 3545
Size: 9040
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2772 4449 6850 278
Size: 53 83 186 936 1236 1677 2401 2468
Fragments arranged by size:
2468 2401 1677 1236 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6383 1937
Size: 4446 4594
Acc65I G'GTAC_C
Cuts at: 4934 6361 4934
Size: 1427 7613
AclI AA'CG_TT
Cuts at: 1563 2655 3588 7167 7540 1563
Size: 1092 933 3579 373 3063
Fragments arranged by size:
3579 3063 1092 933 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2627 2732 3434 5421 5640 7103 8487 8755
Size: 995 105 702 1987 219 1463 1384 268
Cuts at: 8755 1632
Size: 1917
Fragments arranged by size:
1987 1917 1463 1384 995 702 268 219
105
AfeI AGC'GCT
Cuts at: 4581 4921 4581
Size: 340 8700
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8843
AflIII A'CryG_T
Cuts at: 1203 7939 8916 1203
Size: 6736 977 1327
Fragments arranged by size:
6736 1327 977
AgeI A'CCGG_T
Cuts at: 7871 7871
Size: 9040
AhdI GACnn_n'nnGTC
Cuts at: 7769 7769
Size: 9040
AleI CACnn'nnGTG
Cuts at: 1166 6047 1166
Size: 4881 4159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4256 4791 6246 1184
Size: 3072 535 1455 3978
Fragments arranged by size:
3978 3072 1455 535
AlwNI CAG_nnn'CTG
Cuts at: 2682 2710 3200 3700 4306 5183 5909 6783 8355
Size: 28 490 500 606 877 726 874 1572
Cuts at: 8355 2682
Size: 3367
Fragments arranged by size:
3367 1572 877 874 726 606 500 490
28
ApaI G_GGCC'C
Cuts at: 3047 4262 3047
Size: 1215 7825
ApaLI G'TGCA_C
Cuts at: 6050 7097 8253 6050
Size: 1047 1156 6837
Fragments arranged by size:
6837 1156 1047
ApoI r'AATT_y
Cuts at: 1493 4503 5492 6349 6575 6727 8836 1493
Size: 3010 989 857 226 152 2109 1697
Fragments arranged by size:
3010 2109 1697 989 857 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7594 160
Size: 872 6562 1606
Fragments arranged by size:
6562 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9040
AvaI C'yCGr_G
Cuts at: 1870 1943 3906 5102 5282 6365 1870
Size: 73 1963 1196 180 1083 4545
Fragments arranged by size:
4545 1963 1196 1083 180 73
BamHI G'GATC_C
Cuts at: 3094 4132 6370 6737 3094
Size: 1038 2238 367 5397
Fragments arranged by size:
5397 2238 1038 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2564 3376 3608 4934 5038
Size: 325 217 627 777 812 232 1326 104
Cuts at: 5038 5072 5224 6361 7816 618
Size: 34 152 1137 1455 1842
Fragments arranged by size:
1842 1455 1326 1137 812 777 627 325
232 217 152 104 34
BanII G_rGCy'C
Cuts at: 1391 2232 2502 3047 3684 4149 4224 4262 4614
Size: 841 270 545 637 465 75 38 352
Cuts at: 4614 4709 4829 4983 5342 5582 6359 1391
Size: 95 120 154 359 240 777 4072
Fragments arranged by size:
4072 841 777 637 545 465 359 352
270 240 154 120 95 75 38
BbeI G_GCGC'C
Cuts at: 5042 5228 5042
Size: 186 8854
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2887 928
Size: 971 988 7081
Fragments arranged by size:
7081 988 971
BbvCI CC'TCA_GC
Cuts at: 4916 4916
Size: 9040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2674 2708 4334 4368 5722 5756 7231
Size: 34 737 34 1626 34 1354 34 1475
Cuts at: 7231 7265 1903
Size: 34 3678
Fragments arranged by size:
3678 1626 1475 1354 737 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5408 6932 8142 5408
Size: 1524 1210 6306
Fragments arranged by size:
6306 1524 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9040
BfrBI ATG'CAT
Cuts at: 2371 2371
Size: 9040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2402 5126 5262
Size: 107 122 71 631 605 730 2724 136
Cuts at: 5262 7651 136
Size: 2389 1525
Fragments arranged by size:
2724 2389 1525 730 631 605 136 122
107 71
BglII A'GATC_T
Cuts at: 9035 9035
Size: 9040
BlpI GC'TnA_GC
Cuts at: 4049 4328 4619 5910 6457 4049
Size: 279 291 1291 547 6632
Fragments arranged by size:
6632 1291 547 291 279
Bme1580I G_kGCm'C
Cuts at: 1790 3047 3262 3611 4262 6054 7101 8257 1790
Size: 1257 215 349 651 1792 1047 1156 2573
Fragments arranged by size:
2573 1792 1257 1156 1047 651 349 215
BmgBI CAC'GTC
Cuts at: 1318 4652 1318
Size: 3334 5706
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2805 4946 5075
Size: 416 53 83 399 24 1388 2141 129
Cuts at: 5075 6771 7729 442
Size: 1696 958 1753
Fragments arranged by size:
2141 1753 1696 1388 958 416 399 129
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 4238 4846 5596 3911
Size: 327 608 750 7355
Fragments arranged by size:
7355 750 608 327
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2285 4145 4185 4521 4539 4967 5341 5353
Size: 1046 1860 40 336 18 428 374 12
Cuts at: 5353 5703 7700 1239
Size: 350 1997 2579
Fragments arranged by size:
2579 1997 1860 1046 428 374 350 336
40 18 12
Bpu10I CC'TnA_GC
Cuts at: 4608 4916 6005 6273 4608
Size: 308 1089 268 7375
Fragments arranged by size:
7375 1089 308 268
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2111 3311 7165 8030 8328 2111
Size: 1200 3854 865 298 2823
Fragments arranged by size:
3854 2823 1200 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4011 7703 882
Size: 617 2512 3692 2219
Fragments arranged by size:
3692 2512 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3373 3394 6831 493
Size: 711 2169 21 3437 2702
Fragments arranged by size:
3437 2702 2169 711 21
BsaBI GATnn'nnATC
Cuts at: 2684 9034 2684
Size: 6350 2690
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2769 4446 4754 5039
Size: 53 83 186 936 1236 1677 308 285
Cuts at: 5039 5225 6847 7229 7864 275
Size: 186 1622 382 635 1451
Fragments arranged by size:
1677 1622 1451 1236 936 635 382 308
285 186 186 83 53
BsaWI w'CCGG_w
Cuts at: 2507 3619 4904 4931 5026 6111 7473 7871 8145
Size: 1112 1285 27 95 1085 1362 398 274
Cuts at: 8145 8292 8703 2507
Size: 147 411 2844
Fragments arranged by size:
2844 1362 1285 1112 1085 411 398 274
147 95 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4008 4038 4511 4541 5954
Size: 30 165 30 2605 30 473 30 1413
Cuts at: 5954 5984 1178
Size: 30 4234
Fragments arranged by size:
4234 2605 1413 473 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2099 3079 3487 4279 4819 4831 5120
Size: 838 75 980 408 792 540 12 289
Cuts at: 5120 5344 1186
Size: 224 4882
Fragments arranged by size:
4882 980 838 792 540 408 289 224
75 12
BseYI C'CCAG_C
Cuts at: 2672 2703 2991 3693 3879 3986 4262 4355 4627
Size: 31 288 702 186 107 276 93 272
Cuts at: 4627 4885 5045 5156 8243 8739 2672
Size: 258 160 111 3087 496 2973
Fragments arranged by size:
3087 2973 702 496 288 276 272 258
186 160 111 107 93 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2825 3036 3449 4771 1163
Size: 573 345 744 211 413 1322 5432
Fragments arranged by size:
5432 1322 744 573 413 345 211
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4897 6490 7251 8279 1540
Size: 275 186 2896 1593 761 1028 2301
Fragments arranged by size:
2896 2301 1593 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2232 4055 4829 4983 5390 5759 6054 7101 7186
Size: 1823 774 154 407 369 295 1047 85
Cuts at: 7186 8257 2232
Size: 1071 3015
Fragments arranged by size:
3015 1823 1071 1047 774 407 369 295
154 85
BsiWI C'GTAC_G
Cuts at: 3000 3000
Size: 9040
BsmI GAATG_Cn'
Cuts at: 2530 5803 6551 6644 2530
Size: 3273 748 93 4926
Fragments arranged by size:
4926 3273 748 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2389 3396 4448 4667 2389
Size: 1007 1052 219 6762
Fragments arranged by size:
6762 1052 1007 219
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2502 3047 3262 3611 3684 4055
Size: 399 442 270 545 215 349 73 371
Cuts at: 4055 4149 4224 4262 4614 4709 4829 4983 5342
Size: 94 75 38 352 95 120 154 359
Cuts at: 5342 5390 5582 5759 6054 6359 7101 7186 8257
Size: 48 192 177 295 305 742 85 1071
Cuts at: 8257 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 545 442 399 371 359
352 349 305 295 270 215 192 177
154 120 95 94 85 75 73 48
38
BspEI T'CCGG_A
Cuts at: 2507 3619 6111 8703 2507
Size: 1112 2492 2592 2844
Fragments arranged by size:
2844 2592 2492 1112
BspHI T'CATG_A
Cuts at: 6315 6929 8659 6315
Size: 614 1730 6696
Fragments arranged by size:
6696 1730 614
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2103 3471 3545 4428 6395 844
Size: 1259 1368 74 883 1967 3489
Fragments arranged by size:
3489 1967 1368 1259 883 74
BsrBI CCG'CTC
Cuts at: 1394 1823 2754 3655 3661 4893 6927 1394
Size: 429 931 901 6 1232 2034 3507
Fragments arranged by size:
3507 2034 1232 931 901 429 6
BsrDI GCAATG_nn'
Cuts at: 65 1248 2427 5944 7535 7709 65
Size: 1183 1179 3517 1591 174 1396
Fragments arranged by size:
3517 1591 1396 1183 1179 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2072 2595 2698 4037 4896 5978 7684
Size: 110 262 523 103 1339 859 1082 1706
Cuts at: 7684 7871 1700
Size: 187 2869
Fragments arranged by size:
2869 1706 1339 1082 859 523 262 187
110 103
BsrGI T'GTAC_A
Cuts at: 96 2634 2937 96
Size: 2538 303 6199
Fragments arranged by size:
6199 2538 303
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9040
BssSI C'ACGA_G
Cuts at: 7100 8112 7100
Size: 1012 8028
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2682 794
Size: 1888 7152
BstBI TT'CG_AA
Cuts at: 7844 7844
Size: 9040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9040
BstXI CCAn_nnnn'nTGG
Cuts at: 4187 4873 5638 4187
Size: 686 765 7589
Fragments arranged by size:
7589 765 686
BstYI r'GATC_y
Cuts at: 1972 2646 2679 2836 3094 3310 3598 4132 5854
Size: 674 33 157 258 216 288 534 1722
Cuts at: 5854 6370 6737 7122 7139 8591 8677 8689 9035
Size: 516 367 385 17 1452 86 12 346
Cuts at: 9035 1972
Size: 1977
Fragments arranged by size:
1977 1722 1452 674 534 516 385 367
346 288 258 216 157 86 33 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2017 2560 2947 4872 6234 513
Size: 552 939 13 543 387 1925 1362 3319
Fragments arranged by size:
3319 1925 1362 939 552 543 387 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2038 5536 487
Size: 683 56 812 3498 3991
Fragments arranged by size:
3991 3498 812 683 56
BtsI GCAGTG_nn'
Cuts at: 2320 3181 3955 6235 6556 7350 7370 2320
Size: 861 774 2280 321 794 20 3990
Fragments arranged by size:
3990 2280 861 794 774 321 20
ClaI AT'CG_AT
Cuts at: 1554 4494 1554
Size: 2940 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3340 3375 529
Size: 35 2776 35 6194
Fragments arranged by size:
6194 2776 35 35
DraI TTT'AAA
Cuts at: 6344 6344
Size: 9040
DraIII CAC_nnn'GTG
Cuts at: 2953 3376 3472 4690 2953
Size: 423 96 1218 7303
Fragments arranged by size:
7303 1218 423 96
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 8047 809
Size: 507 6731 1802
Fragments arranged by size:
6731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2222 2757 3129
Size: 54 1425 50 275 18 392 535 372
Cuts at: 3129 3184 4894 5127 5628 5640 5691 5980 6487
Size: 55 1710 233 501 12 51 289 507
Cuts at: 6487 7376 9005 8
Size: 889 1629 43
Fragments arranged by size:
1710 1629 1425 889 535 507 501 392
372 289 275 233 55 54 51 50
43 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 4894 6487 1537
Size: 275 3082 1593 4090
Fragments arranged by size:
4090 3082 1593 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3216 4837 6110 6970 1353
Size: 1863 1621 1273 860 3423
Fragments arranged by size:
3423 1863 1621 1273 860
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2768 3712 5088 5543 7627 8001
Size: 1064 138 1179 944 1376 455 2084 374
Cuts at: 8001 8147 387
Size: 146 1280
Fragments arranged by size:
2084 1376 1280 1179 1064 944 455 374
146 138
EcoICRI GAG'CTC
Cuts at: 2230 4827 4981 2230
Size: 2597 154 6289
Fragments arranged by size:
6289 2597 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2285 2627 2732 3434 4145 4185 4521
Size: 393 653 342 105 702 711 40 336
Cuts at: 4521 4539 4967 5341 5353 5421 5640 5703 7103
Size: 18 428 374 12 68 219 63 1400
Cuts at: 7103 7700 8487 8755 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1400 787 711 702 653 597 428
393 374 342 336 268 219 105 68
63 40 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2108 3202 3872 4248 6020 2108
Size: 1094 670 376 1772 5128
Fragments arranged by size:
5128 1772 1094 670 376
EcoO109I rG'GnC_Cy
Cuts at: 2404 3043 3044 3250 3363 3486 3506 3527 4058
Size: 639 1 206 113 123 20 21 531
Cuts at: 4058 4258 4259 4480 5110 5118 5616 6001 2404
Size: 200 1 221 630 8 498 385 5443
Fragments arranged by size:
5443 639 630 531 498 385 221 206
200 123 113 21 20 8 1 1
EcoRI G'AATT_C
Cuts at: 6349 6727 6349
Size: 378 8662
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6124 6124
Size: 9040
FseI GG_CCGG'CC
Cuts at: 4900 4900
Size: 9040
FspI TGC'GCA
Cuts at: 7546 7546
Size: 9040
HaeII r_GCGC'y
Cuts at: 1678 2397 3229 4583 4923 5042 5228 8187 1678
Size: 719 832 1354 340 119 186 2959 2531
Fragments arranged by size:
2959 2531 1354 832 719 340 186 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3328 3360 4666 4698 4733
Size: 32 135 32 1756 32 1306 32 35
Cuts at: 4733 4765 5954 5986 6076 6108 7681 7713 7755
Size: 32 1189 32 90 32 1573 32 42
Cuts at: 7755 7787 1373
Size: 32 2626
Fragments arranged by size:
2626 1756 1573 1306 1189 135 90 42
35 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6298 6384 6630 669
Size: 988 281 4360 86 246 3079
Fragments arranged by size:
4360 3079 988 281 246 86
HindIII A'AGCT_T
Cuts at: 748 2192 748
Size: 1444 7596
HpaI GTT'AAC
Cuts at: 6630 6630
Size: 9040
KasI G'GCGC_C
Cuts at: 5038 5224 5038
Size: 186 8854
KpnI G_GTAC'C
Cuts at: 4938 6365 4938
Size: 1427 7613
MfeI C'AATT_G
Cuts at: 6639 6639
Size: 9040
MluI A'CGCG_T
Cuts at: 8916 8916
Size: 9040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3258 8154 8338 1305
Size: 1953 4896 184 2007
Fragments arranged by size:
4896 2007 1953 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2224 5129 5630 5642 5693
Size: 54 1425 343 392 2905 501 12 51
Cuts at: 5693 10
Size: 3357
Fragments arranged by size:
3357 2905 1425 501 392 343 54 51
12
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2120 2213 2348 3396 5001 5555
Size: 648 891 63 93 135 1048 1605 554
Cuts at: 5555 5636 6047 6998 7357 7516 518
Size: 81 411 951 359 159 2042
Fragments arranged by size:
2042 1605 1048 951 891 648 554 411
359 159 135 93 81 63
MspA1I CmG'CkG
Cuts at: 1606 3286 3846 4366 4442 4618 5167 5382 5668
Size: 1680 560 520 76 176 549 215 286
Cuts at: 5668 5909 6456 7133 8281 8526 1606
Size: 241 547 677 1148 245 2120
Fragments arranged by size:
2120 1680 1148 677 560 549 547 520
286 245 241 215 176 76
NaeI GCC'GGC
Cuts at: 2074 2597 2700 4039 4898 2074
Size: 523 103 1339 859 6216
Fragments arranged by size:
6216 1339 859 523 103
NarI GG'CG_CC
Cuts at: 5039 5225 5039
Size: 186 8854
NcoI C'CATG_G
Cuts at: 513 1065 2004 2017 4872 513
Size: 552 939 13 2855 4681
Fragments arranged by size:
4681 2855 939 552 13
NdeI CA'TA_TG
Cuts at: 387 2264 387
Size: 1877 7163
NgoMIV G'CCGG_C
Cuts at: 2072 2595 2698 4037 4896 2072
Size: 523 103 1339 859 6216
Fragments arranged by size:
6216 1339 859 523 103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9040
NotI GC'GGCC_GC
Cuts at: 6487 6487
Size: 9040
NsiI A_TGCA'T
Cuts at: 2373 2373
Size: 9040
NspI r_CATG'y
Cuts at: 1132 1229 2125 2309 2371 6398 7943 1132
Size: 97 896 184 62 4027 1545 2229
Fragments arranged by size:
4027 2229 1545 896 184 97 62
PasI CC'CwG_GG
Cuts at: 1348 4306 4808 6070 1348
Size: 2958 502 1262 4318
Fragments arranged by size:
4318 2958 1262 502
PciI A'CATG_T
Cuts at: 7939 7939
Size: 9040
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2023 2308 4852 5517 1108
Size: 915 285 2544 665 4631
Fragments arranged by size:
4631 2544 915 665 285
PfoI T'CCnGG_A
Cuts at: 2642 3313 4012 4122 6021 2642
Size: 671 699 110 1899 5661
Fragments arranged by size:
5661 1899 699 671 110
PmeI GTTT'AAAC
Cuts at: 6344 6344
Size: 9040
PmlI CAC'GTG
Cuts at: 3373 3394 3373
Size: 21 9019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7109 7141 8622 8654 7109
Size: 32 1481 32 7495
Fragments arranged by size:
7495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3363 3486 3527 4480 5110 3363
Size: 123 41 953 630 7293
Fragments arranged by size:
7293 953 630 123 41
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9040
PsiI TTA'TAA
Cuts at: 6610 6610
Size: 9040
PspOMI G'GGCC_C
Cuts at: 3043 4258 3043
Size: 1215 7825
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2471 2503 2471
Size: 32 9008
PstI C_TGCA'G
Cuts at: 830 2327 2787 3856 4840 5262 5667 6392 830
Size: 1497 460 1069 984 422 405 725 3478
Fragments arranged by size:
3478 1497 1069 984 725 460 422 405
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9040
PvuII CAG'CTG
Cuts at: 3286 4366 5668 5909 3286
Size: 1080 1302 241 6417
Fragments arranged by size:
6417 1302 1080 241
SacI G_AGCT'C
Cuts at: 2232 4829 4983 2232
Size: 2597 154 6289
Fragments arranged by size:
6289 2597 154
SalI G'TCGA_C
Cuts at: 1936 6382 1936
Size: 4446 4594
SanDI GG'GwC_CC
Cuts at: 3363 3486 3527 3363
Size: 123 41 8876
Fragments arranged by size:
8876 123 41
SapI GCTCTTCn'nnn_
Cuts at: 3216 6110 3216
Size: 2894 6146
SbfI CC_TGCA'GG
Cuts at: 5262 6392 5262
Size: 1130 7910
ScaI AGT'ACT
Cuts at: 7288 9000 7288
Size: 1712 7328
SexAI A'CCwGG_T
Cuts at: 2151 4020 2151
Size: 1869 7171
SfcI C'TryA_G
Cuts at: 826 1045 2105 2323 2783 3192 3852 4836 5258
Size: 219 1060 218 460 409 660 984 422
Cuts at: 5258 5663 6388 7523 8204 8395 826
Size: 405 725 1135 681 191 1471
Fragments arranged by size:
1471 1135 1060 984 725 681 660 460
422 409 405 219 218 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 5126 5262 5126
Size: 136 8904
SfoI GGC'GCC
Cuts at: 5040 5226 5040
Size: 186 8854
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9040
SmaI CCC'GGG
Cuts at: 1872 5284 6367 1872
Size: 3412 1083 4545
Fragments arranged by size:
4545 3412 1083
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 3326 7144 8045 8307 820
Size: 197 926 183 1200 3818 901 262 1553
Fragments arranged by size:
3818 1553 1200 926 901 262 197 183
SnaBI TAC'GTA
Cuts at: 493 6831 493
Size: 6338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9040
SphI G_CATG'C
Cuts at: 1132 2125 6398 1132
Size: 993 4273 3774
Fragments arranged by size:
4273 3774 993
SrfI GCCC'GGGC
Cuts at: 5284 5284
Size: 9040
SspI AAT'ATT
Cuts at: 5 52 6964 5
Size: 47 6912 2081
Fragments arranged by size:
6912 2081 47
StuI AGG'CCT
Cuts at: 1517 3737 5057 5256 1517
Size: 2220 1320 199 5301
Fragments arranged by size:
5301 2220 1320 199
StyI C'CwwG_G
Cuts at: 513 1065 2004 2017 3481 3631 4872 5430 5790
Size: 552 939 13 1464 150 1241 558 360
Cuts at: 5790 5817 6150 6479 513
Size: 27 333 329 3074
Fragments arranged by size:
3074 1464 1241 939 558 552 360 333
329 150 27 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3760 4840 7067 7084 7237 7422 7867
Size: 1255 2180 1080 2227 17 153 185 445
Cuts at: 7867 325
Size: 1498
Fragments arranged by size:
2227 2180 1498 1255 1080 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2634 2937 3354
Size: 274 80 33 51 1443 657 303 417
Cuts at: 3354 7286 8998 96
Size: 3932 1712 138
Fragments arranged by size:
3932 1712 1443 657 417 303 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2240 2272 3601 3633 4511 4543 4894 4926 5643
Size: 32 1329 32 878 32 351 32 717
Cuts at: 5643 5675 6230 6262 9000 9032 2240
Size: 32 555 32 2738 32 2248
Fragments arranged by size:
2738 2248 1329 878 717 555 351 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3490 1316
Size: 2174 6866
XbaI T'CTAG_A
Cuts at: 6376 6376
Size: 9040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2988 3290 3922 4028 5815 2988
Size: 302 632 106 1787 6213
Fragments arranged by size:
6213 1787 632 302 106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9040
XmaI C'CCGG_G
Cuts at: 1870 5282 6365 1870
Size: 3412 1083 4545
Fragments arranged by size:
4545 3412 1083
XmnI GAAnn'nnTTC
Cuts at: 6104 7169 6104
Size: 1065 7975
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2770 4447 6848 276
Size: 53 83 186 936 1236 1677 2401 2468
Fragments arranged by size:
2468 2401 1677 1236 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BstZ17I Bsu36I EcoRV FspAI
NruI PacI PspXI RsrII SacII SwaI