Restriction map for
pFN21AB9566
(Circular) (Six-base) MAPSORT of: pFN21AB9566.seq Check: 2478 from: 1 to: 7120
pFN21AB9566 7120 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4930 278
Size: 53 83 186 936 3394 2468
Fragments arranged by size:
3394 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2811 4081 4463 1937
Size: 874 1270 382 4594
Fragments arranged by size:
4594 1270 874 382
Acc65I G'GTAC_C
Cuts at: 4441 4441
Size: 7120
AclI AA'CG_TT
Cuts at: 1563 5247 5620 1563
Size: 3684 373 3063
Fragments arranged by size:
3684 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2334 2358 2586 4011 4353 5183 6567 6835
Size: 702 24 228 1425 342 830 1384 268
Cuts at: 6835 1632
Size: 1917
Fragments arranged by size:
1917 1425 1384 830 702 342 268 228
24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6923
AflIII A'CryG_T
Cuts at: 1203 2398 2422 2440 2449 2458 2467 3999 4042
Size: 1195 24 18 9 9 9 1532 43
Cuts at: 4042 6019 6996 1203
Size: 1977 977 1327
Fragments arranged by size:
1977 1532 1327 1195 977 43 24 18
9 9 9
AgeI A'CCGG_T
Cuts at: 4332 5951 4332
Size: 1619 5501
AhdI GACnn_n'nnGTC
Cuts at: 2707 5849 2707
Size: 3142 3978
AleI CACnn'nnGTG
Cuts at: 1166 3904 4335 1166
Size: 2738 431 3951
Fragments arranged by size:
3951 2738 431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3888 4085 1184
Size: 2704 197 4219
Fragments arranged by size:
4219 2704 197
AlwNI CAG_nnn'CTG
Cuts at: 2066 4863 6435 2066
Size: 2797 1572 2751
Fragments arranged by size:
2797 2751 1572
ApaLI G'TGCA_C
Cuts at: 3029 5177 6333 3029
Size: 2148 1156 3816
Fragments arranged by size:
3816 2148 1156
ApoI r'AATT_y
Cuts at: 1493 3569 4429 4655 4807 6916 1493
Size: 2076 860 226 152 2109 1697
Fragments arranged by size:
2109 2076 1697 860 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5674 160
Size: 872 4642 1606
Fragments arranged by size:
4642 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
AvaI C'yCGr_G
Cuts at: 1870 1943 4445 1870
Size: 73 2502 4545
Fragments arranged by size:
4545 2502 73
BamHI G'GATC_C
Cuts at: 2589 4450 4817 2589
Size: 1861 367 4892
Fragments arranged by size:
4892 1861 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4441 5896 618
Size: 325 217 627 2654 1455 1842
Fragments arranged by size:
2654 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3693 4439 1391
Size: 2302 746 4072
Fragments arranged by size:
4072 2302 746
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3217 3827 4226 4369 928
Size: 971 1318 610 399 143 3679
Fragments arranged by size:
3679 1318 971 610 399 143
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3338 3372 5311 5345 1903
Size: 34 1401 34 1939 34 3678
Fragments arranged by size:
3678 1939 1401 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3844 5012 6222 3844
Size: 1168 1210 4742
Fragments arranged by size:
4742 1210 1168
BclI T'GATC_A
Cuts at: 1548 2089 2839 2860 1548
Size: 541 750 21 5808
Fragments arranged by size:
5808 750 541 21
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5731 136
Size: 107 122 71 631 605 4059 1525
Fragments arranged by size:
4059 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7115 7115
Size: 7120
BlpI GC'TnA_GC
Cuts at: 4537 4537
Size: 7120
Bme1580I G_kGCm'C
Cuts at: 1790 2240 3033 3145 5181 6337 1790
Size: 450 793 112 2036 1156 2573
Fragments arranged by size:
2573 2036 1156 793 450 112
BmgBI CAC'GTC
Cuts at: 1318 2401 1318
Size: 1083 6037
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3726 4851 5809
Size: 416 53 83 399 24 2309 1125 958
Cuts at: 5809 442
Size: 1753
Fragments arranged by size:
2309 1753 1125 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7120
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2059 2059
Size: 7120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2805 3449 5780 1239
Size: 1566 644 2331 2579
Fragments arranged by size:
2579 2331 1566 644
Bpu10I CC'TnA_GC
Cuts at: 2286 2286
Size: 7120
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3922 4019 5245 6110 6408 3922
Size: 97 1226 865 298 4634
Fragments arranged by size:
4634 1226 865 298 97
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5783 882
Size: 617 4284 2219
Fragments arranged by size:
4284 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4045 4911 493
Size: 711 2841 866 2702
Fragments arranged by size:
2841 2702 866 711
BsaBI GATnn'nnATC
Cuts at: 2088 3261 7114 2088
Size: 1173 3853 2094
Fragments arranged by size:
3853 2094 1173
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4927 5309 5944 275
Size: 53 83 186 936 3394 382 635 1451
Fragments arranged by size:
3394 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3123 4332 5553 5951 6225 6372 6783 3123
Size: 1209 1221 398 274 147 411 3460
Fragments arranged by size:
3460 1221 1209 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3713 3743 1178
Size: 30 165 30 2310 30 4555
Fragments arranged by size:
4555 2310 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3008 1186
Size: 1822 5298
BseYI C'CCAG_C
Cuts at: 6323 6819 6323
Size: 496 6624
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3884 1163
Size: 573 2148 4399
Fragments arranged by size:
4399 2148 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4570 5331 6359 1540
Size: 275 186 2569 761 1028 2301
Fragments arranged by size:
2569 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3033 3693 5181 5266 6337 3033
Size: 660 1488 85 1071 3816
Fragments arranged by size:
3816 1488 1071 660 85
BsmI GAATG_Cn'
Cuts at: 3476 4631 4724 3476
Size: 1155 93 5872
Fragments arranged by size:
5872 1155 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2240 3033 3145 3693 4439 5181 5266
Size: 399 450 793 112 548 746 742 85
Cuts at: 5266 6337 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 793 746 742 548 450 399
112 85
BspEI T'CCGG_A
Cuts at: 6783 6783
Size: 7120
BspHI T'CATG_A
Cuts at: 2476 5009 6739 2476
Size: 2533 1730 2857
Fragments arranged by size:
2857 2533 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4475 844
Size: 3631 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5007 1394
Size: 429 3184 3507
Fragments arranged by size:
3507 3184 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2332 3913 5615 5789 65
Size: 1183 1084 1581 1702 174 1396
Fragments arranged by size:
1702 1581 1396 1183 1084 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4332 5764 5951 1700
Size: 110 2522 1432 187 2869
Fragments arranged by size:
2869 2522 1432 187 110
BsrGI T'GTAC_A
Cuts at: 96 2763 96
Size: 2667 4453
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7120
BssSI C'ACGA_G
Cuts at: 3032 5180 6192 3032
Size: 2148 1012 3960
Fragments arranged by size:
3960 2148 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2558 4390 794
Size: 1764 1832 3524
Fragments arranged by size:
3524 1832 1764
BstBI TT'CG_AA
Cuts at: 3932 5924 3932
Size: 1992 5128
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7120
BstYI r'GATC_y
Cuts at: 1972 2589 4105 4150 4450 4817 5202 5219 6671
Size: 617 1516 45 300 367 385 17 1452
Cuts at: 6671 6757 6769 7115 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1516 1452 617 385 367 346 300
86 45 17 12
Bsu36I CC'TnA_GG
Cuts at: 2748 4282 2748
Size: 1534 5586
BtgI C'CryG_G
Cuts at: 513 1065 2004 2697 513
Size: 552 939 693 4936
Fragments arranged by size:
4936 939 693 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3489 487
Size: 683 56 2263 4118
Fragments arranged by size:
4118 2263 683 56
BtsI GCAGTG_nn'
Cuts at: 3132 4636 5430 5450 3132
Size: 1504 794 20 4802
Fragments arranged by size:
4802 1504 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7120
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7085
DraI TTT'AAA
Cuts at: 2773 2943 4291 4424 2773
Size: 170 1348 133 5469
Fragments arranged by size:
5469 1348 170 133
DraIII CAC_nnn'GTG
Cuts at: 4229 4229
Size: 7120
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6127 809
Size: 507 4811 1802
Fragments arranged by size:
4811 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4567 5456 7085
Size: 54 1425 50 275 18 2737 889 1629
Cuts at: 7085 8
Size: 43
Fragments arranged by size:
2737 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4567 1537
Size: 275 2755 4090
Fragments arranged by size:
4090 2755 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3772 5050 1353
Size: 2419 1278 3423
Fragments arranged by size:
3423 2419 1278
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5707 6081 6227 387
Size: 1064 138 4118 374 146 1280
Fragments arranged by size:
4118 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3691 3691
Size: 7120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2358 2586 2805 3449 4011 4353
Size: 393 702 24 228 219 644 562 342
Cuts at: 4353 5183 5780 6567 6835 1239
Size: 830 597 787 268 1524
Fragments arranged by size:
1524 830 787 702 644 597 562 393
342 268 228 219 24
EcoRI G'AATT_C
Cuts at: 4429 4807 4429
Size: 378 6742
EcoRV GAT'ATC
Cuts at: 2245 2245
Size: 7120
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2036 4372 2036
Size: 2336 4784
FspI TGC'GCA
Cuts at: 5626 5626
Size: 7120
HaeII r_GCGC'y
Cuts at: 1678 6267 1678
Size: 4589 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 2693 2725 3262
Size: 32 135 32 974 32 115 32 537
Cuts at: 3262 3294 4090 4122 5761 5793 5835 5867 1373
Size: 32 796 32 1639 32 42 32 2626
Fragments arranged by size:
2626 1639 974 796 537 135 115 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4464 4710 669
Size: 988 281 2526 246 3079
Fragments arranged by size:
3079 2526 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7120
HpaI GTT'AAC
Cuts at: 4710 4710
Size: 7120
KpnI G_GTAC'C
Cuts at: 4445 4445
Size: 7120
MfeI C'AATT_G
Cuts at: 4719 4719
Size: 7120
MluI A'CGCG_T
Cuts at: 6996 6996
Size: 7120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2416 2425 2434 2691 6234 6418 1305
Size: 1111 9 9 257 3543 184 2007
Fragments arranged by size:
3543 2007 1111 257 184 9 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5298
Fragments arranged by size:
5298 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2082 2153 2481 2759 3156 3651 3820
Size: 648 916 71 328 278 397 495 169
Cuts at: 3820 3904 3993 4162 4335 5078 5437 5596 518
Size: 84 89 169 173 743 359 159 2042
Fragments arranged by size:
2042 916 743 648 495 397 359 328
278 173 169 169 159 89 84 71
MspA1I CmG'CkG
Cuts at: 1606 3014 3375 3590 4536 5213 6361 6606 1606
Size: 1408 361 215 946 677 1148 245 2120
Fragments arranged by size:
2120 1408 1148 946 677 361 245 215
NcoI C'CATG_G
Cuts at: 513 1065 2004 2697 513
Size: 552 939 693 4936
Fragments arranged by size:
4936 939 693 552
NdeI CA'TA_TG
Cuts at: 387 4215 387
Size: 3828 3292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7120
NotI GC'GGCC_GC
Cuts at: 4567 4567
Size: 7120
NspI r_CATG'y
Cuts at: 1132 1229 2426 2444 2453 2462 2471 3788 3875
Size: 97 1197 18 9 9 9 1317 87
Cuts at: 3875 4003 4478 6023 1132
Size: 128 475 1545 2229
Fragments arranged by size:
2229 1545 1317 1197 475 128 97 87
18 9 9 9
PasI CC'CwG_GG
Cuts at: 1348 2886 1348
Size: 1538 5582
PciI A'CATG_T
Cuts at: 2422 2440 2449 2458 2467 3999 6019 2422
Size: 18 9 9 9 1532 2020 3523
Fragments arranged by size:
3523 2020 1532 18 9 9 9
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3619 1108
Size: 2511 4609
PmeI GTTT'AAAC
Cuts at: 4424 4424
Size: 7120
PmlI CAC'GTG
Cuts at: 4045 4045
Size: 7120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5189 5221 6702 6734 5189
Size: 32 1481 32 5575
Fragments arranged by size:
5575 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3831 1613
Size: 2218 4902
PsiI TTA'TAA
Cuts at: 4690 4690
Size: 7120
PstI C_TGCA'G
Cuts at: 830 3007 3763 4472 830
Size: 2177 756 709 3478
Fragments arranged by size:
3478 2177 756 709
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7120
PvuII CAG'CTG
Cuts at: 3590 3590
Size: 7120
SacI G_AGCT'C
Cuts at: 3693 3693
Size: 7120
SalI G'TCGA_C
Cuts at: 1936 4462 1936
Size: 2526 4594
SbfI CC_TGCA'GG
Cuts at: 4472 4472
Size: 7120
ScaI AGT'ACT
Cuts at: 3743 4256 4343 5368 7080 3743
Size: 513 87 1025 1712 3783
Fragments arranged by size:
3783 1712 1025 513 87
SfcI C'TryA_G
Cuts at: 826 1045 2808 3003 3759 4272 4468 5603 6284
Size: 219 1763 195 756 513 196 1135 681
Cuts at: 6284 6475 826
Size: 191 1471
Fragments arranged by size:
1763 1471 1135 756 681 513 219 196
195 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7120
SgrAI Cr'CCGG_yG
Cuts at: 1700 4332 1700
Size: 2632 4488
SmaI CCC'GGG
Cuts at: 1872 4447 1872
Size: 2575 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3937 4034 5224 6125 6387 820
Size: 197 926 1994 97 1190 901 262 1553
Fragments arranged by size:
1994 1553 1190 926 901 262 197 97
SnaBI TAC'GTA
Cuts at: 493 4911 493
Size: 4418 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7120
SphI G_CATG'C
Cuts at: 1132 4478 1132
Size: 3346 3774
SspI AAT'ATT
Cuts at: 5 52 5044 5
Size: 47 4992 2081
Fragments arranged by size:
4992 2081 47
StuI AGG'CCT
Cuts at: 1517 4024 1517
Size: 2507 4613
StyI C'CwwG_G
Cuts at: 513 1065 2004 2649 2697 4559 513
Size: 552 939 645 48 1862 3074
Fragments arranged by size:
3074 1862 939 645 552 48
SwaI ATTT'AAAT
Cuts at: 2943 2943
Size: 7120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5147 5164 5317 5502 5947 325
Size: 1255 3567 17 153 185 445 1498
Fragments arranged by size:
3567 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2107 2763 3377
Size: 274 80 33 51 1443 130 656 614
Cuts at: 3377 3741 4254 4341 5366 7078 96
Size: 364 513 87 1025 1712 138
Fragments arranged by size:
1712 1443 1025 656 614 513 364 274
138 130 87 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4289 4321 7080 7112 4289
Size: 32 2759 32 4297
Fragments arranged by size:
4297 2759 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7120
XbaI T'CTAG_A
Cuts at: 4456 4456
Size: 7120
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2893 2893
Size: 7120
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7120
XmaI C'CCGG_G
Cuts at: 1870 4445 1870
Size: 2575 4545
XmnI GAAnn'nnTTC
Cuts at: 2229 3491 4355 4366 5249 2229
Size: 1262 864 11 883 4100
Fragments arranged by size:
4100 1262 883 864 11
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4928 276
Size: 53 83 186 936 3394 2468
Fragments arranged by size:
3394 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I EcoNI EcoO109I
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI