Restriction map for
pFN21AB9562
(Circular) (Six-base) MAPSORT of: pFN21AB9562.seq Check: 6014 from: 1 to: 7069
pFN21AB9562 7069 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2823 3537 4099 4114 2823
Size: 714 562 15 5778
Fragments arranged by size:
5778 714 562 15
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2526 3189 4879 278
Size: 53 83 186 936 990 663 1690 2468
Fragments arranged by size:
2468 1690 990 936 663 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2012 3657 4412 1937
Size: 75 1645 755 4594
Fragments arranged by size:
4594 1645 755 75
Acc65I G'GTAC_C
Cuts at: 4390 4390
Size: 7069
AclI AA'CG_TT
Cuts at: 1563 5196 5569 1563
Size: 3633 373 3063
Fragments arranged by size:
3633 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3194 5132 6516 6784 1632
Size: 1562 1938 1384 268 1917
Fragments arranged by size:
1938 1917 1562 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6872
AflIII A'CryG_T
Cuts at: 1203 3474 4284 5968 6945 1203
Size: 2271 810 1684 977 1327
Fragments arranged by size:
2271 1684 1327 977 810
AgeI A'CCGG_T
Cuts at: 5900 5900
Size: 7069
AhdI GACnn_n'nnGTC
Cuts at: 5798 5798
Size: 7069
AleI CACnn'nnGTG
Cuts at: 1166 2129 2312 2889 3267 3464 4199 4340 1166
Size: 963 183 577 378 197 735 141 3895
Fragments arranged by size:
3895 963 735 577 378 197 183 141
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2318 1184
Size: 1134 5935
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2975 3005 3007 3037 2975
Size: 30 2 30 7007
Fragments arranged by size:
7007 30 30 2
AlwNI CAG_nnn'CTG
Cuts at: 2966 3115 4114 4812 6384 2966
Size: 149 999 698 1572 3651
Fragments arranged by size:
3651 1572 999 698 149
ApaLI G'TGCA_C
Cuts at: 5126 6282 5126
Size: 1156 5913
ApoI r'AATT_y
Cuts at: 1493 3155 4378 4604 4756 6865 1493
Size: 1662 1223 226 152 2109 1697
Fragments arranged by size:
2109 1697 1662 1223 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5623 160
Size: 872 4591 1606
Fragments arranged by size:
4591 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
AvaI C'yCGr_G
Cuts at: 1870 1943 2372 3231 3246 4255 4394 1870
Size: 73 429 859 15 1009 139 4545
Fragments arranged by size:
4545 1009 859 429 139 73 15
BamHI G'GATC_C
Cuts at: 2799 4399 4766 2799
Size: 1600 367 5102
Fragments arranged by size:
5102 1600 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2152 2921 2933 3423 3744
Size: 325 217 627 365 769 12 490 321
Cuts at: 3744 3791 3836 3911 4193 4390 5845 618
Size: 47 45 75 282 197 1455 1842
Fragments arranged by size:
1842 1455 769 627 490 365 325 321
282 217 197 75 47 45 12
BanII G_rGCy'C
Cuts at: 1391 3765 3809 3939 3981 4043 4388 1391
Size: 2374 44 130 42 62 345 4072
Fragments arranged by size:
4072 2374 345 130 62 44 42
BbeI G_GCGC'C
Cuts at: 3748 3748
Size: 7069
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3265 3355 3583 3601 928
Size: 971 1366 90 228 18 4396
Fragments arranged by size:
4396 1366 971 228 90 18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5260 5294 1903
Size: 34 3323 34 3678
Fragments arranged by size:
3678 3323 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4961 6171 4961
Size: 1210 5859
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7069
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5680 136
Size: 107 122 71 631 605 4008 1525
Fragments arranged by size:
4008 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4134 7064 4134
Size: 2930 4139
BlpI GC'TnA_GC
Cuts at: 4486 4486
Size: 7069
Bme1580I G_kGCm'C
Cuts at: 1790 2373 3348 3426 3794 4221 4262 5130 6286
Size: 583 975 78 368 427 41 868 1156
Cuts at: 6286 1790
Size: 2573
Fragments arranged by size:
2573 1156 975 868 583 427 368 78
41
BmgBI CAC'GTC
Cuts at: 1318 4342 1318
Size: 3024 4045
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3353 4800 5758
Size: 416 53 83 399 24 1936 1447 958
Cuts at: 5758 442
Size: 1753
Fragments arranged by size:
1936 1753 1447 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7069
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2209 3559 3811 2209
Size: 1350 252 5467
Fragments arranged by size:
5467 1350 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2365 2943 3050 3554 3623 3849 5729 1239
Size: 1126 578 107 504 69 226 1880 2579
Fragments arranged by size:
2579 1880 1126 578 504 226 107 69
Bpu10I CC'TnA_GC
Cuts at: 3759 3933 3969 3759
Size: 174 36 6859
Fragments arranged by size:
6859 174 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3014 3473 3827 5194 6059 6357 3014
Size: 459 354 1367 865 298 3726
Fragments arranged by size:
3726 1367 865 459 354 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5732 882
Size: 617 4233 2219
Fragments arranged by size:
4233 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4860 493
Size: 711 3656 2702
Fragments arranged by size:
3656 2702 711
BsaBI GATnn'nnATC
Cuts at: 2952 4139 7063 2952
Size: 1187 2924 2958
Fragments arranged by size:
2958 2924 1187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2523 3186 3745 4293
Size: 53 83 186 936 990 663 559 548
Cuts at: 4293 4876 5258 5893 275
Size: 583 382 635 1451
Fragments arranged by size:
1451 990 936 663 635 583 559 548
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2034 2493 2613 2677 5502 5900 6174 6321 6732
Size: 459 120 64 2825 398 274 147 411
Cuts at: 6732 2034
Size: 2371
Fragments arranged by size:
2825 2371 459 411 398 274 147 120
64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2513 2543 1178
Size: 30 165 30 1110 30 5704
Fragments arranged by size:
5704 1110 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2400 2512 2750 2864 3023 1186
Size: 1214 112 238 114 159 5232
Fragments arranged by size:
5232 1214 238 159 114 112
BseYI C'CCAG_C
Cuts at: 2586 2671 3939 3980 6272 6768 2586
Size: 85 1268 41 2292 496 2887
Fragments arranged by size:
2887 2292 1268 496 85 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2153 2258 3509 1163
Size: 573 417 105 1251 4723
Fragments arranged by size:
4723 1251 573 417 105
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2016 4010 4519 5280 6308 1540
Size: 275 186 15 1994 509 761 1028 2301
Fragments arranged by size:
2301 1994 1028 761 509 275 186 15
BsiHKAI G_wGCw'C
Cuts at: 2504 2868 5130 5215 6286 2504
Size: 364 2262 85 1071 3287
Fragments arranged by size:
3287 2262 1071 364 85
BsmI GAATG_Cn'
Cuts at: 4580 4673 4580
Size: 93 6976
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2373 2504 2868 3348 3426 3765 3794
Size: 399 583 131 364 480 78 339 29
Cuts at: 3794 3809 3939 3981 4043 4221 4262 4388 5130
Size: 15 130 42 62 178 41 126 742
Cuts at: 5130 5215 6286 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 583 480 399 364 339
178 131 130 126 85 78 62 42
41 29 15
BspEI T'CCGG_A
Cuts at: 2034 2677 6732 2034
Size: 643 4055 2371
Fragments arranged by size:
4055 2371 643
BspHI T'CATG_A
Cuts at: 4095 4958 6688 4095
Size: 863 1730 4476
Fragments arranged by size:
4476 1730 863
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2466 2673 2823 3084 3393 3537 3711 4099
Size: 1622 207 150 261 309 144 174 388
Cuts at: 4099 4114 4424 844
Size: 15 310 3489
Fragments arranged by size:
3489 1622 388 310 309 261 207 174
150 144 15
BsrBI CCG'CTC
Cuts at: 1394 1823 3294 3301 4956 1394
Size: 429 1471 7 1655 3507
Fragments arranged by size:
3507 1655 1471 429 7
BsrDI GCAATG_nn'
Cuts at: 65 1248 2448 5564 5738 65
Size: 1183 1200 3116 174 1396
Fragments arranged by size:
3116 1396 1200 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2155 2298 3747 3776 5713 5900 1700
Size: 110 345 143 1449 29 1937 187 2869
Fragments arranged by size:
2869 1937 1449 345 187 143 110 29
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7069
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7069
BssSI C'ACGA_G
Cuts at: 5129 6141 5129
Size: 1012 6057
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7069
BstBI TT'CG_AA
Cuts at: 5873 5873
Size: 7069
BstEII G'GTnAC_C
Cuts at: 1184 3505 1184
Size: 2321 4748
BstXI CCAn_nnnn'nTGG
Cuts at: 2425 3638 3856 2425
Size: 1213 218 5638
Fragments arranged by size:
5638 1213 218
BstYI r'GATC_y
Cuts at: 1972 2166 2799 3096 4134 4170 4399 4766 5151
Size: 194 633 297 1038 36 229 367 385
Cuts at: 5151 5168 6620 6706 6718 7064 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1038 633 385 367 346 297
229 194 86 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 2699 2721 2940 3348 3501
Size: 552 939 69 626 22 219 408 153
Cuts at: 3501 3708 3798 513
Size: 207 90 3784
Fragments arranged by size:
3784 939 626 552 408 219 207 153
90 69 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6330
Fragments arranged by size:
6330 683 56
BtsI GCAGTG_nn'
Cuts at: 2905 4040 4260 4585 5379 5399 2905
Size: 1135 220 325 794 20 4575
Fragments arranged by size:
4575 1135 794 325 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7069
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7034
DraI TTT'AAA
Cuts at: 4373 4373
Size: 7069
DraIII CAC_nnn'GTG
Cuts at: 2211 2772 2211
Size: 561 6508
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3200 3359 3889 4162 4322 6076 809
Size: 507 1884 159 530 273 160 1754 1802
Fragments arranged by size:
1884 1802 1754 530 507 273 160 159
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2427 2445
Size: 54 1425 50 275 18 372 225 18
Cuts at: 2445 3696 4516 5405 7034 8
Size: 1251 820 889 1629 43
Fragments arranged by size:
1629 1425 1251 889 820 372 275 225
54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4516 1537
Size: 275 2704 4090
Fragments arranged by size:
4090 2704 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2178 2394 2468 2753 2825 3131 3167 3170
Size: 825 216 74 285 72 306 36 3
Cuts at: 3170 3218 3449 3566 4999 1353
Size: 48 231 117 1433 3423
Fragments arranged by size:
3423 1433 825 306 285 231 216 117
74 72 48 36 3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3766 5656 6030 6176 387
Size: 1064 138 2177 1890 374 146 1280
Fragments arranged by size:
2177 1890 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2365 2943 3050 3194 3554 3623 3849
Size: 393 733 578 107 144 360 69 226
Cuts at: 3849 5132 5729 6516 6784 1239
Size: 1283 597 787 268 1524
Fragments arranged by size:
1524 1283 787 733 597 578 393 360
268 226 144 107 69
EcoNI CCTnn'n_nnAGG
Cuts at: 2846 3533 3602 2846
Size: 687 69 6313
Fragments arranged by size:
6313 687 69
EcoO109I rG'GnC_Cy
Cuts at: 2335 2421 2963 3372 3733 4057 4150 2335
Size: 86 542 409 361 324 93 5254
Fragments arranged by size:
5254 542 409 361 324 93 86
EcoRI G'AATT_C
Cuts at: 4378 4756 4378
Size: 378 6691
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2047 2047
Size: 7069
FspI TGC'GCA
Cuts at: 5575 5575
Size: 7069
HaeII r_GCGC'y
Cuts at: 1678 3748 6216 1678
Size: 2070 2468 2531
Fragments arranged by size:
2531 2468 2070
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2513 2545 3629 3661 3752
Size: 32 135 32 941 32 1084 32 91
Cuts at: 3752 3784 5710 5742 5784 5816 1373
Size: 32 1926 32 42 32 2626
Fragments arranged by size:
2626 1926 1084 941 135 91 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2013 3472 4413 4659 669
Size: 988 281 75 1459 941 246 3079
Fragments arranged by size:
3079 1459 988 941 281 246 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7069
HpaI GTT'AAC
Cuts at: 4659 4659
Size: 7069
KasI G'GCGC_C
Cuts at: 3744 3744
Size: 7069
KpnI G_GTAC'C
Cuts at: 4394 4394
Size: 7069
MfeI C'AATT_G
Cuts at: 4668 4668
Size: 7069
MluI A'CGCG_T
Cuts at: 6945 6945
Size: 7069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2220 3078 3672 6183 6367 1305
Size: 915 858 594 2511 184 2007
Fragments arranged by size:
2511 2007 915 858 594 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2204 2447 3698 10
Size: 54 1425 343 372 243 1251 3381
Fragments arranged by size:
3381 1425 1251 372 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2129 2312 2889 2939 3267 3395 3464
Size: 648 963 183 577 50 328 128 69
Cuts at: 3464 3797 4199 4265 4283 4340 5027 5386 5545
Size: 333 402 66 18 57 687 359 159
Cuts at: 5545 518
Size: 2042
Fragments arranged by size:
2042 963 687 648 577 402 359 333
328 183 159 128 69 66 57 50
18
MspA1I CmG'CkG
Cuts at: 1606 2541 3420 3430 3694 3742 3818 4003 4084
Size: 935 879 10 264 48 76 185 81
Cuts at: 4084 4363 4485 5162 6310 6555 1606
Size: 279 122 677 1148 245 2120
Fragments arranged by size:
2120 1148 935 879 677 279 264 245
185 122 81 76 48 10
NaeI GCC'GGC
Cuts at: 2157 2300 3749 2157
Size: 143 1449 5477
Fragments arranged by size:
5477 1449 143
NarI GG'CG_CC
Cuts at: 3745 3745
Size: 7069
NcoI C'CATG_G
Cuts at: 513 1065 2004 2699 2940 3348 3501 3798 513
Size: 552 939 695 241 408 153 297 3784
Fragments arranged by size:
3784 939 695 552 408 297 241 153
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7069
NgoMIV G'CCGG_C
Cuts at: 2155 2298 3747 2155
Size: 143 1449 5477
Fragments arranged by size:
5477 1449 143
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7069
NotI GC'GGCC_GC
Cuts at: 4516 4516
Size: 7069
NspI r_CATG'y
Cuts at: 1132 1229 2417 2646 2738 4288 4427 5972 1132
Size: 97 1188 229 92 1550 139 1545 2229
Fragments arranged by size:
2229 1550 1545 1188 229 139 97 92
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7069
PciI A'CATG_T
Cuts at: 4284 5968 4284
Size: 1684 5385
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2533 2700 3552 3586 3694 1108
Size: 1425 167 852 34 108 4483
Fragments arranged by size:
4483 1425 852 167 108 34
PfoI T'CCnGG_A
Cuts at: 3600 4064 3600
Size: 464 6605
PmeI GTTT'AAAC
Cuts at: 4373 4373
Size: 7069
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5138 5170 6651 6683 5138
Size: 32 1481 32 5524
Fragments arranged by size:
5524 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 3372 4057 2335
Size: 1037 685 5347
Fragments arranged by size:
5347 1037 685
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7069
PsiI TTA'TAA
Cuts at: 4639 4639
Size: 7069
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 7037
PstI C_TGCA'G
Cuts at: 830 2297 3741 3829 4089 4110 4188 4421 830
Size: 1467 1444 88 260 21 78 233 3478
Fragments arranged by size:
3478 1467 1444 260 233 88 78 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7069
PvuII CAG'CTG
Cuts at: 2541 3694 2541
Size: 1153 5916
SalI G'TCGA_C
Cuts at: 1936 2011 4411 1936
Size: 75 2400 4594
Fragments arranged by size:
4594 2400 75
SanDI GG'GwC_CC
Cuts at: 3372 3372
Size: 7069
SapI GCTCTTCn'nnn_
Cuts at: 2468 2825 3170 2468
Size: 357 345 6367
Fragments arranged by size:
6367 357 345
SbfI CC_TGCA'GG
Cuts at: 4421 4421
Size: 7069
ScaI AGT'ACT
Cuts at: 5317 7029 5317
Size: 1712 5357
SexAI A'CCwGG_T
Cuts at: 3551 3585 3651 3551
Size: 34 66 6969
Fragments arranged by size:
6969 66 34
SfcI C'TryA_G
Cuts at: 826 1045 2293 3737 3825 4085 4106 4184 4417
Size: 219 1248 1444 88 260 21 78 233
Cuts at: 4417 5552 6233 6424 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1444 1248 1135 681 260 233 219
191 88 78 21
SfoI GGC'GCC
Cuts at: 3746 3746
Size: 7069
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7069
SgrAI Cr'CCGG_yG
Cuts at: 1700 3747 1700
Size: 2047 5022
SmaI CCC'GGG
Cuts at: 1872 2374 3233 4396 1872
Size: 502 859 1163 4545
Fragments arranged by size:
4545 1163 859 502
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3029 3488 3842 5173 6074 6336
Size: 197 926 1086 459 354 1331 901 262
Cuts at: 6336 820
Size: 1553
Fragments arranged by size:
1553 1331 1086 926 901 459 354 262
197
SnaBI TAC'GTA
Cuts at: 493 4860 493
Size: 4367 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7069
SphI G_CATG'C
Cuts at: 1132 2417 4427 1132
Size: 1285 2010 3774
Fragments arranged by size:
3774 2010 1285
SrfI GCCC'GGGC
Cuts at: 3233 3233
Size: 7069
SspI AAT'ATT
Cuts at: 5 52 4993 5
Size: 47 4941 2081
Fragments arranged by size:
4941 2081 47
StuI AGG'CCT
Cuts at: 1517 3539 1517
Size: 2022 5047
StyI C'CwwG_G
Cuts at: 513 1065 2004 2699 2940 3348 3501 3798 4508
Size: 552 939 695 241 408 153 297 710
Cuts at: 4508 513
Size: 3074
Fragments arranged by size:
3074 939 710 695 552 408 297 241
153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2030 3899 5096 5113 5266 5451 5896
Size: 1255 450 1869 1197 17 153 185 445
Cuts at: 5896 325
Size: 1498
Fragments arranged by size:
1869 1498 1255 1197 450 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5315 7027 96
Size: 274 80 33 51 1443 3338 1712 138
Fragments arranged by size:
3338 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7029 7061 7029
Size: 32 7037
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7069
XbaI T'CTAG_A
Cuts at: 4405 4405
Size: 7069
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2911 4050 2911
Size: 1139 5930
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7069
XmaI C'CCGG_G
Cuts at: 1870 2372 3231 4394 1870
Size: 502 859 1163 4545
Fragments arranged by size:
4545 1163 859 502
XmnI GAAnn'nnTTC
Cuts at: 5198 5198
Size: 7069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2524 3187 4877 276
Size: 53 83 186 936 990 663 1690 2468
Fragments arranged by size:
2468 1690 990 936 663 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SfiI SwaI