Restriction map for
pFN21AB9560
(Circular) (Six-base) MAPSORT of: pFN21AB9560.seq Check: 2853 from: 1 to: 8113
pFN21AB9560 8113 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5923 278
Size: 53 83 186 936 4387 2468
Fragments arranged by size:
4387 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5456 1937
Size: 3519 4594
Acc65I G'GTAC_C
Cuts at: 3855 4242 5434 3855
Size: 387 1192 6534
Fragments arranged by size:
6534 1192 387
AclI AA'CG_TT
Cuts at: 1563 6240 6613 1563
Size: 4677 373 3063
Fragments arranged by size:
4677 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2673 3517 3889 4343 4430 4570 4577 4610
Size: 1041 844 372 454 87 140 7 33
Cuts at: 4610 4995 5182 6176 7560 7828 1632
Size: 385 187 994 1384 268 1917
Fragments arranged by size:
1917 1384 1041 994 844 454 385 372
268 187 140 87 33 7
AfeI AGC'GCT
Cuts at: 2117 4337 2117
Size: 2220 5893
AflII C'TTAA_G
Cuts at: 820 1017 2672 820
Size: 197 1655 6261
Fragments arranged by size:
6261 1655 197
AflIII A'CryG_T
Cuts at: 1203 3510 5369 7012 7989 1203
Size: 2307 1859 1643 977 1327
Fragments arranged by size:
2307 1859 1643 1327 977
AgeI A'CCGG_T
Cuts at: 2178 6944 2178
Size: 4766 3347
AhdI GACnn_n'nnGTC
Cuts at: 5065 6842 5065
Size: 1777 6336
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8113
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4542 1184
Size: 3358 4755
AlwNI CAG_nnn'CTG
Cuts at: 3416 4529 5856 7428 3416
Size: 1113 1327 1572 4101
Fragments arranged by size:
4101 1572 1327 1113
ApaLI G'TGCA_C
Cuts at: 6170 7326 6170
Size: 1156 6957
ApoI r'AATT_y
Cuts at: 1493 2230 2522 2752 2928 4020 4308 4580 5422
Size: 737 292 230 176 1092 288 272 842
Cuts at: 5422 5648 5800 7909 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1092 842 737 292 288 272
230 226 176 152
AseI AT'TA_AT
Cuts at: 160 1032 3216 6667 160
Size: 872 2184 3451 1606
Fragments arranged by size:
3451 2184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8113
AvaI C'yCGr_G
Cuts at: 1870 1943 2583 2874 5438 1870
Size: 73 640 291 2564 4545
Fragments arranged by size:
4545 2564 640 291 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5097 5130 5097
Size: 33 8080
BamHI G'GATC_C
Cuts at: 3537 5443 5810 3537
Size: 1906 367 5840
Fragments arranged by size:
5840 1906 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2645 3855 4242 5434 6889
Size: 325 217 627 858 1210 387 1192 1455
Cuts at: 6889 618
Size: 1842
Fragments arranged by size:
1842 1455 1210 1192 858 627 387 325
217
BanII G_rGCy'C
Cuts at: 1391 2249 2661 5126 5168 5432 1391
Size: 858 412 2465 42 264 4072
Fragments arranged by size:
4072 2465 858 412 264 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4101 928
Size: 971 2202 4940
Fragments arranged by size:
4940 2202 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6304 6338 1903
Size: 34 4367 34 3678
Fragments arranged by size:
4367 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6005 7215 6005
Size: 1210 6903
BclI T'GATC_A
Cuts at: 1548 2950 5206 1548
Size: 1402 2256 4455
Fragments arranged by size:
4455 2256 1402
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6724 136
Size: 107 122 71 631 605 5052 1525
Fragments arranged by size:
5052 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8108 8108
Size: 8113
BlpI GC'TnA_GC
Cuts at: 5530 5530
Size: 8113
Bme1580I G_kGCm'C
Cuts at: 1790 6174 7330 1790
Size: 4384 1156 2573
Fragments arranged by size:
4384 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3513 1318
Size: 2195 5918
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 2855 5844
Size: 416 53 83 399 24 917 521 2989
Cuts at: 5844 6802 442
Size: 958 1753
Fragments arranged by size:
2989 1753 958 917 521 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8113
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5034 5210 5034
Size: 176 7937
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4136 6773 1239
Size: 2897 2637 2579
Fragments arranged by size:
2897 2637 2579
Bpu10I CC'TnA_GC
Cuts at: 3033 3033
Size: 8113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2093 2699 4690 6238 7103 7401 2093
Size: 606 1991 1548 865 298 2805
Fragments arranged by size:
2805 1991 1548 865 606 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 3416 3606 6776 882
Size: 617 585 1332 190 3170 2219
Fragments arranged by size:
3170 2219 1332 617 585 190
BsaAI yAC'GTr
Cuts at: 493 1204 4565 5904 493
Size: 711 3361 1339 2702
Fragments arranged by size:
3361 2702 1339 711
BsaBI GATnn'nnATC
Cuts at: 4061 8107 4061
Size: 4046 4067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5920 6302 6937 275
Size: 53 83 186 936 4387 382 635 1451
Fragments arranged by size:
4387 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2178 6546 6944 7218 7365 7776 2178
Size: 4368 398 274 147 411 2515
Fragments arranged by size:
4368 2515 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2391 2421 1178
Size: 30 165 30 988 30 6870
Fragments arranged by size:
6870 988 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2482 2565 2651 3293 4430 4517 1186
Size: 916 380 83 86 642 1137 87 4782
Fragments arranged by size:
4782 1137 916 642 380 87 86 83
BseYI C'CCAG_C
Cuts at: 7316 7812 7316
Size: 496 7617
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7540
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5563 6324 7352 1540
Size: 275 186 3562 761 1028 2301
Fragments arranged by size:
3562 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2661 3494 5126 6174 6259 7330 2661
Size: 833 1632 1048 85 1071 3444
Fragments arranged by size:
3444 1632 1071 1048 833 85
BsmI GAATG_Cn'
Cuts at: 2776 2972 5624 5717 2776
Size: 196 2652 93 5172
Fragments arranged by size:
5172 2652 196 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3519 4396 5396 3519
Size: 877 1000 6236
Fragments arranged by size:
6236 1000 877
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2249 2661 3494 5126 5168 5432 6174
Size: 399 459 412 833 1632 42 264 742
Cuts at: 6174 6259 7330 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1632 1071 833 742 459 412 399
264 85 42
BspEI T'CCGG_A
Cuts at: 7776 7776
Size: 8113
BspHI T'CATG_A
Cuts at: 2800 6002 7732 2800
Size: 3202 1730 3181
Fragments arranged by size:
3202 3181 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 3069 5468 844
Size: 1841 384 2399 3489
Fragments arranged by size:
3489 2399 1841 384
BsrBI CCG'CTC
Cuts at: 1394 1823 6000 1394
Size: 429 4177 3507
Fragments arranged by size:
4177 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4359 4968 6608 6782 65
Size: 1183 3111 609 1640 174 1396
Fragments arranged by size:
3111 1640 1396 1183 609 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 2942 6757 6944 1700
Size: 110 368 764 3815 187 2869
Fragments arranged by size:
3815 2869 764 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8113
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8113
BssSI C'ACGA_G
Cuts at: 6173 7185 6173
Size: 1012 7101
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8113
BstBI TT'CG_AA
Cuts at: 6917 6917
Size: 8113
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8113
BstXI CCAn_nnnn'nTGG
Cuts at: 5069 5354 5069
Size: 285 7828
BstYI r'GATC_y
Cuts at: 1972 2282 3537 4466 5443 5810 6195 6212 7664
Size: 310 1255 929 977 367 385 17 1452
Cuts at: 7664 7750 7762 8108 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1255 977 929 385 367 346
310 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4100 4100
Size: 8113
BtgI C'CryG_G
Cuts at: 513 1065 2004 3735 4026 513
Size: 552 939 1731 291 4600
Fragments arranged by size:
4600 1731 939 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7374
Fragments arranged by size:
7374 683 56
BtsI GCAGTG_nn'
Cuts at: 2841 5209 5629 6423 6443 2841
Size: 2368 420 794 20 4511
Fragments arranged by size:
4511 2368 794 420 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3611 3646 529
Size: 35 3047 35 4996
Fragments arranged by size:
4996 3047 35 35
DraI TTT'AAA
Cuts at: 2533 5417 2533
Size: 2884 5229
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7120 809
Size: 507 5804 1802
Fragments arranged by size:
5804 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2721 3375
Size: 54 1425 50 275 18 177 714 654
Cuts at: 3375 3738 4125 5223 5560 6449 8078 8
Size: 363 387 1098 337 889 1629 43
Fragments arranged by size:
1629 1425 1098 889 714 654 387 363
337 275 177 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5560 1537
Size: 275 3748 4090
Fragments arranged by size:
4090 3748 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2649 3264 3384 4815 4850 5003 5201 6043
Size: 1296 615 120 1431 35 153 198 842
Cuts at: 6043 1353
Size: 3423
Fragments arranged by size:
3423 1431 1296 842 615 198 153 120
35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4252 6700 7074 7220 387
Size: 1064 138 2663 2448 374 146 1280
Fragments arranged by size:
2663 2448 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2659 5124 2659
Size: 2465 5648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2673 3517 3889 4136 4343 4430 4570
Size: 393 1041 844 372 247 207 87 140
Cuts at: 4570 4577 4610 4995 5182 6176 6773 7560 7828
Size: 7 33 385 187 994 597 787 268
Cuts at: 7828 1239
Size: 1524
Fragments arranged by size:
1524 1041 994 844 787 597 393 385
372 268 247 207 187 140 87 33
7
EcoNI CCTnn'n_nnAGG
Cuts at: 3711 4216 5159 3711
Size: 505 943 6665
Fragments arranged by size:
6665 943 505
EcoO109I rG'GnC_Cy
Cuts at: 4000 4380 4000
Size: 380 7733
EcoRI G'AATT_C
Cuts at: 5422 5800 5422
Size: 378 7735
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2444 2134
Size: 310 7803
FspI TGC'GCA
Cuts at: 4230 6619 4230
Size: 2389 5724
HaeII r_GCGC'y
Cuts at: 1678 2119 4339 7260 1678
Size: 441 2220 2921 2531
Fragments arranged by size:
2921 2531 2220 441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 5051 5083 6754
Size: 32 135 32 905 32 2542 32 1671
Cuts at: 6754 6786 6828 6860 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2542 1671 905 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2170 3586 5457 5703 669
Size: 988 281 232 1416 1871 246 3079
Fragments arranged by size:
3079 1871 1416 988 281 246 232
HindIII A'AGCT_T
Cuts at: 748 2360 2454 2675 748
Size: 1612 94 221 6186
Fragments arranged by size:
6186 1612 221 94
HpaI GTT'AAC
Cuts at: 2170 5703 2170
Size: 3533 4580
KpnI G_GTAC'C
Cuts at: 3859 4246 5438 3859
Size: 387 1192 6534
Fragments arranged by size:
6534 1192 387
MfeI C'AATT_G
Cuts at: 5712 5712
Size: 8113
MluI A'CGCG_T
Cuts at: 7989 7989
Size: 8113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2463 4274 7227 7411 1305
Size: 1158 1811 2953 184 2007
Fragments arranged by size:
2953 2007 1811 1158 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 3377 3740 4127 5225
Size: 54 1425 343 177 1368 363 387 1098
Cuts at: 5225 10
Size: 2898
Fragments arranged by size:
2898 1425 1368 1098 387 363 343 177
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3746 6071 6430 6589 518
Size: 648 2580 2325 359 159 2042
Fragments arranged by size:
2580 2325 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2384 2635 3528 5529 6206 7354 7599 1606
Size: 778 251 893 2001 677 1148 245 2120
Fragments arranged by size:
2120 2001 1148 893 778 677 251 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6622
Fragments arranged by size:
6622 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8113
NotI GC'GGCC_GC
Cuts at: 5560 5560
Size: 8113
NspI r_CATG'y
Cuts at: 1132 1229 4436 4523 5373 5471 7016 1132
Size: 97 3207 87 850 98 1545 2229
Fragments arranged by size:
3207 2229 1545 850 98 97 87
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8113
PciI A'CATG_T
Cuts at: 5369 7012 5369
Size: 1643 6470
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8113
PfoI T'CCnGG_A
Cuts at: 2448 2448
Size: 8113
PmeI GTTT'AAAC
Cuts at: 5417 5417
Size: 8113
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6182 6214 7695 7727 6182
Size: 32 1481 32 6568
Fragments arranged by size:
6568 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4000 4380 4000
Size: 380 7733
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8113
PsiI TTA'TAA
Cuts at: 5683 5683
Size: 8113
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2415 2447 2415
Size: 32 8081
PstI C_TGCA'G
Cuts at: 830 5465 830
Size: 4635 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8113
PvuII CAG'CTG
Cuts at: 2384 2635 3528 2384
Size: 251 893 6969
Fragments arranged by size:
6969 893 251
SacI G_AGCT'C
Cuts at: 2661 5126 2661
Size: 2465 5648
SalI G'TCGA_C
Cuts at: 1936 5455 1936
Size: 3519 4594
SanDI GG'GwC_CC
Cuts at: 4380 4380
Size: 8113
SapI GCTCTTCn'nnn_
Cuts at: 2649 3264 2649
Size: 615 7498
SbfI CC_TGCA'GG
Cuts at: 5465 5465
Size: 8113
ScaI AGT'ACT
Cuts at: 6361 8073 6361
Size: 1712 6401
SexAI A'CCwGG_T
Cuts at: 3567 3567
Size: 8113
SfcI C'TryA_G
Cuts at: 826 1045 3073 3708 3758 5461 6596 7277 7468
Size: 219 2028 635 50 1703 1135 681 191
Cuts at: 7468 826
Size: 1471
Fragments arranged by size:
2028 1703 1471 1135 681 635 219 191
50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8113
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8113
SmaI CCC'GGG
Cuts at: 1872 5440 1872
Size: 3568 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2108 2672 2678 2874 4705 6217
Size: 197 926 165 564 6 196 1831 1512
Cuts at: 6217 7118 7380 820
Size: 901 262 1553
Fragments arranged by size:
1831 1553 1512 926 901 564 262 197
196 165 6
SnaBI TAC'GTA
Cuts at: 493 4565 5904 493
Size: 4072 1339 2702
Fragments arranged by size:
4072 2702 1339
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8113
SphI G_CATG'C
Cuts at: 1132 4523 5471 1132
Size: 3391 948 3774
Fragments arranged by size:
3774 3391 948
SspI AAT'ATT
Cuts at: 5 52 5275 6037 5
Size: 47 5223 762 2081
Fragments arranged by size:
5223 2081 762 47
StuI AGG'CCT
Cuts at: 1517 3703 3914 1517
Size: 2186 211 5716
Fragments arranged by size:
5716 2186 211
StyI C'CwwG_G
Cuts at: 513 1065 2004 4894 5109 5552 513
Size: 552 939 2890 215 443 3074
Fragments arranged by size:
3074 2890 939 552 443 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3143 3473 6140 6157 6310 6495 6940
Size: 1255 1563 330 2667 17 153 185 445
Cuts at: 6940 325
Size: 1498
Fragments arranged by size:
2667 1563 1498 1255 445 330 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5012 6359 8071
Size: 274 80 33 51 1443 3035 1347 1712
Cuts at: 8071 96
Size: 138
Fragments arranged by size:
3035 1712 1443 1347 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 8073 8105 3377
Size: 32 4664 32 3385
Fragments arranged by size:
4664 3385 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8113
XbaI T'CTAG_A
Cuts at: 2495 2887 2962 5449 2495
Size: 392 75 2487 5159
Fragments arranged by size:
5159 2487 392 75
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2446 2782 3548 3991 2011
Size: 435 336 766 443 6133
Fragments arranged by size:
6133 766 443 435 336
XhoI C'TCGA_G
Cuts at: 1943 2874 1943
Size: 931 7182
XmaI C'CCGG_G
Cuts at: 1870 5438 1870
Size: 3568 4545
XmnI GAAnn'nnTTC
Cuts at: 4583 6242 4583
Size: 1659 6454
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5921 276
Size: 53 83 186 936 4387 2468
Fragments arranged by size:
4387 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI BfrBI
BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI
RsrII SacII SfiI SfoI SrfI SwaI