Restriction map for
pFN21AB9559
(Circular) (Six-base) MAPSORT of: pFN21AB9559.seq Check: 8610 from: 1 to: 7963
pFN21AB9559 7963 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3826 5773 278
Size: 53 83 186 936 2290 1947 2468
Fragments arranged by size:
2468 2290 1947 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3949 4219 5306 1937
Size: 2012 270 1087 4594
Fragments arranged by size:
4594 2012 1087 270
Acc65I G'GTAC_C
Cuts at: 4262 5284 4262
Size: 1022 6941
AclI AA'CG_TT
Cuts at: 1563 2977 6090 6463 1563
Size: 1414 3113 373 3063
Fragments arranged by size:
3113 3063 1414 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 4628 6026 7410 7678 1632
Size: 845 2151 1398 1384 268 1917
Fragments arranged by size:
2151 1917 1398 1384 845 268
AfeI AGC'GCT
Cuts at: 3389 3389
Size: 7963
AflII C'TTAA_G
Cuts at: 820 1017 2050 820
Size: 197 1033 6733
Fragments arranged by size:
6733 1033 197
AflIII A'CryG_T
Cuts at: 1203 2423 3741 4938 6862 7839 1203
Size: 1220 1318 1197 1924 977 1327
Fragments arranged by size:
1924 1327 1318 1220 1197 977
AgeI A'CCGG_T
Cuts at: 2186 6794 2186
Size: 4608 3355
AhdI GACnn_n'nnGTC
Cuts at: 6692 6692
Size: 7963
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7963
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7963
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3918 3950 4530 4562 4632 4664 3918
Size: 32 580 32 70 32 7217
Fragments arranged by size:
7217 580 70 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2864 4714 4828 5706 7278 2864
Size: 1850 114 878 1572 3549
Fragments arranged by size:
3549 1850 1572 878 114
ApaI G_GGCC'C
Cuts at: 3446 3446
Size: 7963
ApaLI G'TGCA_C
Cuts at: 6020 7176 6020
Size: 1156 6807
ApoI r'AATT_y
Cuts at: 1493 2355 3074 4025 4161 4915 5033 5084 5272
Size: 862 719 951 136 754 118 51 188
Cuts at: 5272 5498 5650 7759 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 862 754 719 226 188
152 136 118 51
AseI AT'TA_AT
Cuts at: 160 1032 2088 2730 6517 160
Size: 872 1056 642 3787 1606
Fragments arranged by size:
3787 1606 1056 872 642
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7963
AvaI C'yCGr_G
Cuts at: 1870 1943 3662 5024 5288 1870
Size: 73 1719 1362 264 4545
Fragments arranged by size:
4545 1719 1362 264 73
BamHI G'GATC_C
Cuts at: 2407 5293 5660 2407
Size: 2886 367 4710
Fragments arranged by size:
4710 2886 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4262 4421 5284 6739 618
Size: 325 217 627 2475 159 863 1455 1842
Fragments arranged by size:
2475 1842 1455 863 627 325 217 159
BanII G_rGCy'C
Cuts at: 1391 2696 3446 5282 1391
Size: 1305 750 1836 4072
Fragments arranged by size:
4072 1836 1305 750
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2031 2420 3858 4941 928
Size: 971 132 389 1438 1083 3950
Fragments arranged by size:
3950 1438 1083 971 389 132
BbvCI CC'TCA_GC
Cuts at: 3573 4875 3573
Size: 1302 6661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6154 6188 1903
Size: 34 4217 34 3678
Fragments arranged by size:
4217 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5011 5855 7065 5011
Size: 844 1210 5909
Fragments arranged by size:
5909 1210 844
BclI T'GATC_A
Cuts at: 1548 4273 1548
Size: 2725 5238
BfrBI ATG'CAT
Cuts at: 2346 3817 2346
Size: 1471 6492
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3646 6574 136
Size: 107 122 71 631 605 1974 2928 1525
Fragments arranged by size:
2928 1974 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5050 7958 5050
Size: 2908 5055
BlpI GC'TnA_GC
Cuts at: 5380 5380
Size: 7963
Bme1580I G_kGCm'C
Cuts at: 1790 3446 6024 7180 1790
Size: 1656 2578 1156 2573
Fragments arranged by size:
2578 2573 1656 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4115 5694 6652
Size: 416 53 83 399 24 2698 1579 958
Cuts at: 6652 442
Size: 1753
Fragments arranged by size:
2698 1753 1579 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4200 1056
Size: 3144 4819
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2375 2375
Size: 7963
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 2537 3147 3987 4850 6623 1239
Size: 1106 192 610 840 863 1773 2579
Fragments arranged by size:
2579 1773 1106 863 840 610 192
Bpu10I CC'TnA_GC
Cuts at: 3129 3573 4875 3129
Size: 444 1302 6217
Fragments arranged by size:
6217 1302 444
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3004 6088 6953 7251 3004
Size: 3084 865 298 3716
Fragments arranged by size:
3716 3084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2195 2584 6626 882
Size: 617 696 389 4042 2219
Fragments arranged by size:
4042 2219 696 617 389
BsaAI yAC'GTr
Cuts at: 493 1204 5754 493
Size: 711 4550 2702
Fragments arranged by size:
4550 2702 711
BsaBI GATnn'nnATC
Cuts at: 7957 7957
Size: 7963
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3823 4765 5770 6152
Size: 53 83 186 936 2290 942 1005 382
Cuts at: 6152 6787 275
Size: 635 1451
Fragments arranged by size:
2290 1451 1005 942 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2186 6396 6794 7068 7215 7626 2186
Size: 4210 398 274 147 411 2523
Fragments arranged by size:
4210 2523 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2502 2532 3523 3553 3918
Size: 30 165 30 1099 30 991 30 365
Cuts at: 3918 3948 3960 3990 1178
Size: 30 12 30 5151
Fragments arranged by size:
5151 1099 991 365 165 30 30 30
30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3531 3939 5145 1186
Size: 2345 408 1206 4004
Fragments arranged by size:
4004 2345 1206 408
BseYI C'CCAG_C
Cuts at: 2258 3604 4213 4859 7166 7662 2258
Size: 1346 609 646 2307 496 2559
Fragments arranged by size:
2559 2307 1346 646 609 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4356 4418 1163
Size: 573 2620 62 4708
Fragments arranged by size:
4708 2620 573 62
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5413 6174 7202 1540
Size: 275 186 3412 761 1028 2301
Fragments arranged by size:
3412 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2331 2696 2825 4099 6024 6109 7180 2331
Size: 365 129 1274 1925 85 1071 3114
Fragments arranged by size:
3114 1925 1274 1071 365 129 85
BsmI GAATG_Cn'
Cuts at: 3684 3787 5474 5567 3684
Size: 103 1687 93 6080
Fragments arranged by size:
6080 1687 103 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2035 2035
Size: 7963
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2696 2825 3446 4099 5282 6024
Size: 399 541 365 129 621 653 1183 742
Cuts at: 6024 6109 7180 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1183 1071 742 653 621 541 399
365 129 85
BspEI T'CCGG_A
Cuts at: 7626 7626
Size: 7963
BspHI T'CATG_A
Cuts at: 5852 7582 5852
Size: 1730 6233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5318 844
Size: 4474 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5850 1394
Size: 429 4027 3507
Fragments arranged by size:
4027 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2159 6458 6632 65
Size: 1183 911 4299 174 1396
Fragments arranged by size:
4299 1396 1183 911 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 3870 6607 6794 1700
Size: 110 376 1684 2737 187 2869
Fragments arranged by size:
2869 2737 1684 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7963
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7963
BssSI C'ACGA_G
Cuts at: 6023 7035 6023
Size: 1012 6951
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7963
BstBI TT'CG_AA
Cuts at: 6767 6767
Size: 7963
BstEII G'GTnAC_C
Cuts at: 1184 2216 1184
Size: 1032 6931
BstXI CCAn_nnnn'nTGG
Cuts at: 4213 5011 4213
Size: 798 7165
BstYI r'GATC_y
Cuts at: 1972 2232 2407 2785 3013 3151 4647 5050 5293
Size: 260 175 378 228 138 1496 403 243
Cuts at: 5293 5660 6045 6062 7514 7600 7612 7958 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1496 1452 403 385 378 367 346
260 243 228 175 138 86 17 12
BstZ17I GTA'TAC
Cuts at: 4220 4220
Size: 7963
Bsu36I CC'TnA_GG
Cuts at: 2545 2545
Size: 7963
BtgI C'CryG_G
Cuts at: 513 1065 2004 4629 4651 513
Size: 552 939 2625 22 3825
Fragments arranged by size:
3825 2625 939 552 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3316 487
Size: 683 56 2090 5134
Fragments arranged by size:
5134 2090 683 56
BtsI GCAGTG_nn'
Cuts at: 2511 5479 6273 6293 2511
Size: 2968 794 20 4181
Fragments arranged by size:
4181 2968 794 20
ClaI AT'CG_AT
Cuts at: 1554 4773 1554
Size: 3219 4744
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7928
DraI TTT'AAA
Cuts at: 2974 5267 2974
Size: 2293 5670
DraIII CAC_nnn'GTG
Cuts at: 4697 4697
Size: 7963
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2639 5063 6970 809
Size: 507 1323 2424 1907 1802
Fragments arranged by size:
2424 1907 1802 1323 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3286 5410 6299
Size: 54 1425 50 275 18 1456 2124 889
Cuts at: 6299 7928 8
Size: 1629 43
Fragments arranged by size:
2124 1629 1456 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5410 1537
Size: 275 3598 4090
Fragments arranged by size:
4090 3598 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4008 4291 5893 1353
Size: 2655 283 1602 3423
Fragments arranged by size:
3423 2655 1602 283
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4671 6550 6924 7070 387
Size: 1064 138 3082 1879 374 146 1280
Fragments arranged by size:
3082 1879 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2694 2694
Size: 7963
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2477 2537 3147 3987 4628 4850
Size: 393 713 132 60 610 840 641 222
Cuts at: 4850 6026 6623 7410 7678 1239
Size: 1176 597 787 268 1524
Fragments arranged by size:
1524 1176 840 787 713 641 610 597
393 268 222 132 60
EcoO109I rG'GnC_Cy
Cuts at: 2262 2313 3442 4238 2262
Size: 51 1129 796 5987
Fragments arranged by size:
5987 1129 796 51
EcoRI G'AATT_C
Cuts at: 2355 5272 5650 2355
Size: 2917 378 4668
Fragments arranged by size:
4668 2917 378
EcoRV GAT'ATC
Cuts at: 3191 3191
Size: 7963
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4687 4687
Size: 7963
FspI TGC'GCA
Cuts at: 6469 6469
Size: 7963
HaeII r_GCGC'y
Cuts at: 1678 3391 7110 1678
Size: 1713 3719 2531
Fragments arranged by size:
3719 2531 1713
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2564 2596 3556 3588 6604
Size: 32 135 32 992 32 960 32 3016
Cuts at: 6604 6636 6678 6710 1373
Size: 32 42 32 2626
Fragments arranged by size:
3016 2626 992 960 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2157 4126 5307 5553 669
Size: 988 281 219 1969 1181 246 3079
Fragments arranged by size:
3079 1969 1181 988 281 246 219
HindIII A'AGCT_T
Cuts at: 748 3591 748
Size: 2843 5120
HpaI GTT'AAC
Cuts at: 5553 5553
Size: 7963
KpnI G_GTAC'C
Cuts at: 4266 5288 4266
Size: 1022 6941
MfeI C'AATT_G
Cuts at: 5562 5562
Size: 7963
MluI A'CGCG_T
Cuts at: 7839 7839
Size: 7963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2109 2204 7077 7261 1305
Size: 804 95 4873 184 2007
Fragments arranged by size:
4873 2007 804 184 95
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6141
Fragments arranged by size:
6141 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5921 6280 6439 518
Size: 648 4755 359 159 2042
Fragments arranged by size:
4755 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3041 3224 3604 4687 5379 6056 7204 7449
Size: 1435 183 380 1083 692 677 1148 245
Cuts at: 7449 1606
Size: 2120
Fragments arranged by size:
2120 1435 1148 1083 692 677 380 245
183
NcoI C'CATG_G
Cuts at: 513 1065 2004 4629 513
Size: 552 939 2625 3847
Fragments arranged by size:
3847 2625 939 552
NdeI CA'TA_TG
Cuts at: 387 3520 387
Size: 3133 4830
NheI G'CTAG_C
Cuts at: 1052 4196 1052
Size: 3144 4819
NotI GC'GGCC_GC
Cuts at: 5410 5410
Size: 7963
NsiI A_TGCA'T
Cuts at: 2348 3819 2348
Size: 1471 6492
NspI r_CATG'y
Cuts at: 1132 1229 2211 2427 3745 4597 5181 5321 6866
Size: 97 982 216 1318 852 584 140 1545
Cuts at: 6866 1132
Size: 2229
Fragments arranged by size:
2229 1545 1318 982 852 584 216 140
97
PasI CC'CwG_GG
Cuts at: 1348 5161 1348
Size: 3813 4150
PciI A'CATG_T
Cuts at: 2423 3741 6862 2423
Size: 1318 3121 3524
Fragments arranged by size:
3524 3121 1318
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2323 4630 1108
Size: 1215 2307 4441
Fragments arranged by size:
4441 2307 1215
PfoI T'CCnGG_A
Cuts at: 2235 3846 2235
Size: 1611 6352
PmeI GTTT'AAAC
Cuts at: 5267 5267
Size: 7963
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3918 3950 6032 6064 7545 7577 3918
Size: 32 2082 32 1481 32 4304
Fragments arranged by size:
4304 2082 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2313 2313
Size: 7963
PshAI GACnn'nnGTC
Cuts at: 1613 5250 1613
Size: 3637 4326
PsiI TTA'TAA
Cuts at: 4494 5533 4494
Size: 1039 6924
PspOMI G'GGCC_C
Cuts at: 3442 3442
Size: 7963
PstI C_TGCA'G
Cuts at: 830 3792 4731 5315 830
Size: 2962 939 584 3478
Fragments arranged by size:
3478 2962 939 584
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7963
PvuII CAG'CTG
Cuts at: 3041 3224 3604 4687 3041
Size: 183 380 1083 6317
Fragments arranged by size:
6317 1083 380 183
SacI G_AGCT'C
Cuts at: 2696 2696
Size: 7963
SalI G'TCGA_C
Cuts at: 1936 5305 1936
Size: 3369 4594
SapI GCTCTTCn'nnn_
Cuts at: 4008 4291 4008
Size: 283 7680
SbfI CC_TGCA'GG
Cuts at: 5315 5315
Size: 7963
ScaI AGT'ACT
Cuts at: 3168 3209 6211 7923 3168
Size: 41 3002 1712 3208
Fragments arranged by size:
3208 3002 1712 41
SfcI C'TryA_G
Cuts at: 826 1045 3788 3950 4291 4727 5311 6446 7127
Size: 219 2743 162 341 436 584 1135 681
Cuts at: 7127 7318 826
Size: 191 1471
Fragments arranged by size:
2743 1471 1135 681 584 436 341 219
191 162
SfiI GGCCn_nnn'nGGCC
Cuts at: 3646 3646
Size: 7963
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7963
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7963
SmaI CCC'GGG
Cuts at: 1872 5290 1872
Size: 3418 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2050 2983 6067 6968 7230 820
Size: 197 926 107 933 3084 901 262 1553
Fragments arranged by size:
3084 1553 933 926 901 262 197 107
SnaBI TAC'GTA
Cuts at: 493 5754 493
Size: 5261 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7963
SphI G_CATG'C
Cuts at: 1132 4597 5321 1132
Size: 3465 724 3774
Fragments arranged by size:
3774 3465 724
SspI AAT'ATT
Cuts at: 5 52 3806 4951 5887 5
Size: 47 3754 1145 936 2081
Fragments arranged by size:
3754 2081 1145 936 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7963
StyI C'CwwG_G
Cuts at: 513 1065 2004 4629 4671 5402 513
Size: 552 939 2625 42 731 3074
Fragments arranged by size:
3074 2625 939 731 552 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 4409 4520 4827 5990 6007 6160
Size: 1255 2589 240 111 307 1163 17 153
Cuts at: 6160 6345 6790 325
Size: 185 445 1498
Fragments arranged by size:
2589 1498 1255 1163 445 307 240 185
153 111 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3136 3166 3207
Size: 274 80 33 51 1443 1159 30 41
Cuts at: 3207 4965 5008 6209 7921 96
Size: 1758 43 1201 1712 138
Fragments arranged by size:
1758 1712 1443 1201 1159 274 138 80
51 43 41 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2502 2534 3684 3716 7923 7955 2502
Size: 32 1150 32 4207 32 2510
Fragments arranged by size:
4207 2510 1150 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7963
XbaI T'CTAG_A
Cuts at: 2719 5053 5299 2719
Size: 2334 246 5383
Fragments arranged by size:
5383 2334 246
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7963
XmaI C'CCGG_G
Cuts at: 1870 5288 1870
Size: 3418 4545
XmnI GAAnn'nnTTC
Cuts at: 3558 4017 4164 6092 3558
Size: 459 147 1928 5429
Fragments arranged by size:
5429 1928 459 147
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3824 5771 276
Size: 53 83 186 936 2290 1947 2468
Fragments arranged by size:
2468 2290 1947 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BsiWI EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI
SwaI XcmI