Restriction map for
pFN21AB9557
(Circular) (Six-base) MAPSORT of: pFN21AB9557.seq Check: 3952 from: 1 to: 10717
pFN21AB9557 10717 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6625 6625
Size: 10717
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3016 4679 8527 278
Size: 53 83 186 936 1480 1663 3848 2468
Fragments arranged by size:
3848 2468 1663 1480 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2935 8060 1937
Size: 998 5125 4594
Fragments arranged by size:
5125 4594 998
Acc65I G'GTAC_C
Cuts at: 8038 8038
Size: 10717
AclI AA'CG_TT
Cuts at: 1563 2371 4636 8844 9217 1563
Size: 808 2265 4208 373 3063
Fragments arranged by size:
4208 3063 2265 808 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2070 3046 3336 4268 4665 4898 4939 5054
Size: 438 976 290 932 397 233 41 115
Cuts at: 5054 5151 5733 6637 7240 7472 8780 10164 10432
Size: 97 582 904 603 232 1308 1384 268
Cuts at: 10432 1632
Size: 1917
Fragments arranged by size:
1917 1384 1308 976 932 904 603 582
438 397 290 268 233 232 115 97
41
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10520
AflIII A'CryG_T
Cuts at: 1203 4535 6147 7497 9616 10593 1203
Size: 3332 1612 1350 2119 977 1327
Fragments arranged by size:
3332 2119 1612 1350 1327 977
AgeI A'CCGG_T
Cuts at: 9548 9548
Size: 10717
AhdI GACnn_n'nnGTC
Cuts at: 9446 9446
Size: 10717
AleI CACnn'nnGTG
Cuts at: 1166 5132 6152 1166
Size: 3966 1020 5731
Fragments arranged by size:
5731 3966 1020
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6875 1184
Size: 5691 5026
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6734 6766 6734
Size: 32 10685
AlwNI CAG_nnn'CTG
Cuts at: 2342 2805 5927 6370 6735 6849 7172 7279 7624
Size: 463 3122 443 365 114 323 107 345
Cuts at: 7624 8460 10032 2342
Size: 836 1572 3027
Fragments arranged by size:
3122 3027 1572 836 463 443 365 345
323 114 107
ApaLI G'TGCA_C
Cuts at: 8774 9930 8774
Size: 1156 9561
ApoI r'AATT_y
Cuts at: 1493 3248 3558 3626 4291 5407 8026 8252 8404
Size: 1755 310 68 665 1116 2619 226 152
Cuts at: 8404 10513 1493
Size: 2109 1697
Fragments arranged by size:
2619 2109 1755 1697 1116 665 310 226
152 68
AseI AT'TA_AT
Cuts at: 160 1032 9271 160
Size: 872 8239 1606
Fragments arranged by size:
8239 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10717
AvaI C'yCGr_G
Cuts at: 1870 1943 3282 5663 8042 1870
Size: 73 1339 2381 2379 4545
Fragments arranged by size:
4545 2381 2379 1339 73
AvrII C'CTAG_G
Cuts at: 2939 2939
Size: 10717
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7177 7210 7177
Size: 33 10684
BamHI G'GATC_C
Cuts at: 6554 8047 8414 6554
Size: 1493 367 8857
Fragments arranged by size:
8857 1493 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6326 7223 8038 9493 618
Size: 325 217 627 4539 897 815 1455 1842
Fragments arranged by size:
4539 1842 1455 897 815 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3231 4842 5577 8036 1391
Size: 1840 1611 735 2459 4072
Fragments arranged by size:
4072 2459 1840 1611 735
BbeI G_GCGC'C
Cuts at: 6330 6330
Size: 10717
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2106 4562 4671 4825 5262 5655 6204
Size: 971 207 2456 109 154 437 393 549
Cuts at: 6204 6426 928
Size: 222 5219
Fragments arranged by size:
5219 2456 971 549 437 393 222 207
154 109
BbvCI CC'TCA_GC
Cuts at: 4386 7059 4386
Size: 2673 8044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8908 8942 1903
Size: 34 6971 34 3678
Fragments arranged by size:
6971 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7860 8609 9819 7860
Size: 749 1210 8758
Fragments arranged by size:
8758 1210 749
BclI T'GATC_A
Cuts at: 1548 7152 1548
Size: 5604 5113
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7658 9328 136
Size: 107 122 71 631 605 5986 1670 1525
Fragments arranged by size:
5986 1670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 10712 10712
Size: 10717
BlpI GC'TnA_GC
Cuts at: 7380 8134 7380
Size: 754 9963
Bme1580I G_kGCm'C
Cuts at: 1790 7566 7983 8778 9934 1790
Size: 5776 417 795 1156 2573
Fragments arranged by size:
5776 2573 1156 795 417
BmgBI CAC'GTC
Cuts at: 1318 6150 1318
Size: 4832 5885
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6930 7191 8448
Size: 416 53 83 399 24 5513 261 1257
Cuts at: 8448 9406 442
Size: 958 1753
Fragments arranged by size:
5513 1753 1257 958 416 399 261 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10717
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4871 4871
Size: 10717
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2556 4023 4320 4506 5748 5996 6772 9377
Size: 1317 1467 297 186 1242 248 776 2605
Cuts at: 9377 1239
Size: 2579
Fragments arranged by size:
2605 2579 1467 1317 1242 776 297 248
186
Bpu10I CC'TnA_GC
Cuts at: 2253 4386 7059 7402 2253
Size: 2133 2673 343 5568
Fragments arranged by size:
5568 2673 2133 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3573 3583 4161 4841 5438 5541 6285 6539 7401
Size: 10 578 680 597 103 744 254 862
Cuts at: 7401 8842 9707 10005 3573
Size: 1441 865 298 4285
Fragments arranged by size:
4285 1441 865 862 744 680 597 578
298 254 103 10
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4041 4366 5117 5783 6581 9380 882
Size: 617 2542 325 751 666 798 2799 2219
Fragments arranged by size:
2799 2542 2219 798 751 666 617 325
BsaAI yAC'GTr
Cuts at: 493 1204 8508 493
Size: 711 7304 2702
Fragments arranged by size:
7304 2702 711
BsaBI GATnn'nnATC
Cuts at: 4311 10711 4311
Size: 6400 4317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3013 4676 6327 7436
Size: 53 83 186 936 1480 1663 1651 1109
Cuts at: 7436 8524 8906 9541 275
Size: 1088 382 635 1451
Fragments arranged by size:
1663 1651 1480 1451 1109 1088 936 635
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 9150 9548 9822 9969 10380 9150
Size: 398 274 147 411 9487
Fragments arranged by size:
9487 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5037 5067 7129 7159 1178
Size: 30 165 30 3634 30 2062 30 4736
Fragments arranged by size:
4736 3634 2062 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3419 3565 4382 4394 4677 4790 4985 4988
Size: 2233 146 817 12 283 113 195 3
Cuts at: 4988 4991 4994 5080 5231 5403 5681 5693 5696
Size: 3 3 86 151 172 278 12 3
Cuts at: 5696 5699 5831 5861 5879 5882 5900 5915 5918
Size: 3 132 30 18 3 18 15 3
Cuts at: 5918 6047 6143 6251 6445 7404 7470 1186
Size: 129 96 108 194 959 66 4433
Fragments arranged by size:
4433 2233 959 817 283 278 195 194
172 151 146 132 129 113 108 96
86 66 30 18 18 15 12 12
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3381 4605 5752 6173 6473 7029 7065 7092 7141
Size: 1224 1147 421 300 556 36 27 49
Cuts at: 7141 7406 7896 9920 10416 3381
Size: 265 490 2024 496 3682
Fragments arranged by size:
3682 2024 1224 1147 556 496 490 421
300 265 49 36 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4903 6308 1163
Size: 573 3167 1405 5572
Fragments arranged by size:
5572 3167 1405 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8167 8928 9956 1540
Size: 275 186 6166 761 1028 2301
Fragments arranged by size:
6166 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 6680 8778 8863 9934 6680
Size: 2098 85 1071 7463
Fragments arranged by size:
7463 2098 1071 85
BsmI GAATG_Cn'
Cuts at: 4215 7518 7768 8228 8321 4215
Size: 3303 250 460 93 6611
Fragments arranged by size:
6611 3303 460 250 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4667 4667
Size: 10717
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3231 4842 5577 6680 7566 7983 8036
Size: 399 1441 1611 735 1103 886 417 53
Cuts at: 8036 8778 8863 9934 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1611 1441 1103 1071 886 742 735
417 399 85 53
BspEI T'CCGG_A
Cuts at: 10380 10380
Size: 10717
BspHI T'CATG_A
Cuts at: 3432 4459 4561 8606 10336 3432
Size: 1027 102 4045 1730 3813
Fragments arranged by size:
4045 3813 1730 1027 102
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3972 6625 8072 844
Size: 3128 2653 1447 3489
Fragments arranged by size:
3489 3128 2653 1447
BsrBI CCG'CTC
Cuts at: 1394 1823 3160 7578 8604 1394
Size: 429 1337 4418 1026 3507
Fragments arranged by size:
4418 3507 1337 1026 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2110 3199 9212 9386 65
Size: 1183 862 1089 6013 174 1396
Fragments arranged by size:
6013 1396 1183 1089 862 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2605 7226 9361 9548 1700
Size: 110 795 4621 2135 187 2869
Fragments arranged by size:
4621 2869 2135 795 187 110
BsrGI T'GTAC_A
Cuts at: 96 6277 7935 96
Size: 6181 1658 2878
Fragments arranged by size:
6181 2878 1658
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10717
BssSI C'ACGA_G
Cuts at: 6013 8777 9789 6013
Size: 2764 1012 6941
Fragments arranged by size:
6941 2764 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5927 6849 7537 7906 794
Size: 5133 922 688 369 3605
Fragments arranged by size:
5133 3605 922 688 369
BstBI TT'CG_AA
Cuts at: 3073 9521 3073
Size: 6448 4269
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10717
BstXI CCAn_nnnn'nTGG
Cuts at: 3890 4357 5490 3890
Size: 467 1133 9117
Fragments arranged by size:
9117 1133 467
BstYI r'GATC_y
Cuts at: 1972 2221 4312 5613 6265 6554 8047 8414 8799
Size: 249 2091 1301 652 289 1493 367 385
Cuts at: 8799 8816 10268 10354 10366 10712 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
2091 1977 1493 1452 1301 652 385 367
346 289 249 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4689 4869 4946 5808 4689
Size: 180 77 862 9598
Fragments arranged by size:
9598 862 180 77
BtgI C'CryG_G
Cuts at: 513 1065 2004 2122 2266 2473 6153 6411 513
Size: 552 939 118 144 207 3680 258 4819
Fragments arranged by size:
4819 3680 939 552 258 207 144 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9978
Fragments arranged by size:
9978 683 56
BtsI GCAGTG_nn'
Cuts at: 2422 2783 3479 3913 5104 5749 6291 6319 7592
Size: 361 696 434 1191 645 542 28 1273
Cuts at: 7592 8233 9027 9047 2422
Size: 641 794 20 4092
Fragments arranged by size:
4092 1273 1191 794 696 645 641 542
434 361 28 20
ClaI AT'CG_AT
Cuts at: 1554 3993 5205 1554
Size: 2439 1212 7066
Fragments arranged by size:
7066 2439 1212
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2511 2546 7495 7530 529
Size: 35 1947 35 4949 35 3716
Fragments arranged by size:
4949 3716 1947 35 35 35
DraI TTT'AAA
Cuts at: 2187 2730 8021 2187
Size: 543 5291 4883
Fragments arranged by size:
5291 4883 543
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4940 9724 809
Size: 507 3624 4784 1802
Fragments arranged by size:
4784 3624 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2470 2713 5968
Size: 54 1425 50 275 18 640 243 3255
Cuts at: 5968 6831 7630 8164 9053 10682 8
Size: 863 799 534 889 1629 43
Fragments arranged by size:
3255 1629 1425 889 863 799 640 534
275 243 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8164 1537
Size: 275 6352 4090
Fragments arranged by size:
6352 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4371 4398 4665 5208 5217 5305 5331 5700
Size: 3018 27 267 543 9 88 26 369
Cuts at: 5700 5709 5721 5820 6071 6120 6210 6237 6754
Size: 9 12 99 251 49 90 27 517
Cuts at: 6754 8008 8647 1353
Size: 1254 639 3423
Fragments arranged by size:
3423 3018 1254 639 543 517 369 267
251 99 90 88 49 27 27 26
12 9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2877 3289 4235 9304 9678 9824
Size: 1064 138 1288 412 946 5069 374 146
Cuts at: 9824 387
Size: 1280
Fragments arranged by size:
5069 1288 1280 1064 946 412 374 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2070 2556 3046 3336 4023 4268 4320
Size: 393 438 486 490 290 687 245 52
Cuts at: 4320 4506 4665 4898 4939 5054 5151 5733 5748
Size: 186 159 233 41 115 97 582 15
Cuts at: 5748 5996 6637 6772 7240 7472 8780 9377 10164
Size: 248 641 135 468 232 1308 597 787
Cuts at: 10164 10432 1239
Size: 268 1524
Fragments arranged by size:
1524 1308 787 687 641 597 582 490
486 468 438 393 290 268 248 245
233 232 186 159 135 115 97 52
41 15
EcoNI CCTnn'n_nnAGG
Cuts at: 5663 5663
Size: 10717
EcoO109I rG'GnC_Cy
Cuts at: 3056 6397 7943 3056
Size: 3341 1546 5830
Fragments arranged by size:
5830 3341 1546
EcoRI G'AATT_C
Cuts at: 5407 8026 8404 5407
Size: 2619 378 7720
Fragments arranged by size:
7720 2619 378
EcoRV GAT'ATC
Cuts at: 3466 3466
Size: 10717
FspI TGC'GCA
Cuts at: 9223 9223
Size: 10717
HaeII r_GCGC'y
Cuts at: 1678 2995 6330 9864 1678
Size: 1317 3335 3534 2531
Fragments arranged by size:
3534 3335 2531 1317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2439 2471 3940 3972 5147
Size: 32 135 32 867 32 1469 32 1175
Cuts at: 5147 5179 5324 5356 7705 7737 9358 9390 9432
Size: 32 145 32 2349 32 1621 32 42
Cuts at: 9432 9464 1373
Size: 32 2626
Fragments arranged by size:
2626 2349 1621 1469 1175 867 145 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2936 4161 8061 8307 669
Size: 988 281 998 1225 3900 246 3079
Fragments arranged by size:
3900 3079 1225 998 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4280 748
Size: 3532 7185
HpaI GTT'AAC
Cuts at: 8307 8307
Size: 10717
KasI G'GCGC_C
Cuts at: 6326 6326
Size: 10717
KpnI G_GTAC'C
Cuts at: 8042 8042
Size: 10717
MfeI C'AATT_G
Cuts at: 2153 8316 2153
Size: 6163 4554
MluI A'CGCG_T
Cuts at: 10593 10593
Size: 10717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3472 4374 9831 10015 1305
Size: 2167 902 5457 184 2007
Fragments arranged by size:
5457 2167 2007 902 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2472 2715 5970 6833 10
Size: 54 1425 343 640 243 3255 863 3894
Fragments arranged by size:
3894 3255 1425 863 640 343 243 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2900 4122 5132 6152 7292 7463 7697
Size: 648 1734 1222 1010 1020 1140 171 234
Cuts at: 7697 7748 8675 9034 9193 518
Size: 51 927 359 159 2042
Fragments arranged by size:
2042 1734 1222 1140 1020 1010 927 648
359 234 171 159 51
MspA1I CmG'CkG
Cuts at: 1606 3525 4288 4605 4762 5918 6648 6762 6810
Size: 1919 763 317 157 1156 730 114 48
Cuts at: 6810 6849 7108 7279 7384 7624 8133 8810 9958
Size: 39 259 171 105 240 509 677 1148
Cuts at: 9958 10203 1606
Size: 245 2120
Fragments arranged by size:
2120 1919 1156 1148 763 730 677 509
317 259 245 240 171 157 114 105
48 39
NaeI GCC'GGC
Cuts at: 7228 7228
Size: 10717
NarI GG'CG_CC
Cuts at: 6327 6327
Size: 10717
NcoI C'CATG_G
Cuts at: 513 1065 2004 2122 2266 6411 513
Size: 552 939 118 144 4145 4819
Fragments arranged by size:
4819 4145 939 552 144 118
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10717
NgoMIV G'CCGG_C
Cuts at: 7226 7226
Size: 10717
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10717
NotI GC'GGCC_GC
Cuts at: 8164 8164
Size: 10717
NspI r_CATG'y
Cuts at: 1132 1229 2799 2839 2917 5565 5656 7501 8075
Size: 97 1570 40 78 2648 91 1845 574
Cuts at: 8075 9620 1132
Size: 1545 2229
Fragments arranged by size:
2648 2229 1845 1570 1545 574 97 91
78 40
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10717
PciI A'CATG_T
Cuts at: 7497 9616 7497
Size: 2119 8598
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2128 7172 1108
Size: 1020 5044 4653
Fragments arranged by size:
5044 4653 1020
PfoI T'CCnGG_A
Cuts at: 4263 5616 4263
Size: 1353 9364
PmeI GTTT'AAAC
Cuts at: 8021 8021
Size: 10717
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8786 8818 10299 10331 8786
Size: 32 1481 32 9172
Fragments arranged by size:
9172 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3056 6397 3056
Size: 3341 7376
PshAI GACnn'nnGTC
Cuts at: 1613 4337 1613
Size: 2724 7993
PsiI TTA'TAA
Cuts at: 8287 8287
Size: 10717
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7793 7825 7919 7951 7793
Size: 32 94 32 10559
Fragments arranged by size:
10559 94 32 32
PstI C_TGCA'G
Cuts at: 830 3185 3486 3666 5923 6375 6854 6896 7275
Size: 2355 301 180 2257 452 479 42 379
Cuts at: 7275 8069 830
Size: 794 3478
Fragments arranged by size:
3478 2355 2257 794 479 452 379 301
180 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10717
PvuII CAG'CTG
Cuts at: 4288 4762 5918 6762 6810 6849 7279 7384 7624
Size: 474 1156 844 48 39 430 105 240
Cuts at: 7624 4288
Size: 7381
Fragments arranged by size:
7381 1156 844 474 430 240 105 48
39
SalI G'TCGA_C
Cuts at: 1936 2934 8059 1936
Size: 998 5125 4594
Fragments arranged by size:
5125 4594 998
SapI GCTCTTCn'nnn_
Cuts at: 4398 6754 4398
Size: 2356 8361
SbfI CC_TGCA'GG
Cuts at: 8069 8069
Size: 10717
ScaI AGT'ACT
Cuts at: 8965 10677 8965
Size: 1712 9005
SexAI A'CCwGG_T
Cuts at: 3566 5503 3566
Size: 1937 8780
SfcI C'TryA_G
Cuts at: 826 1045 2629 3181 3482 3662 4302 4921 5919
Size: 219 1584 552 301 180 640 619 998
Cuts at: 5919 6371 6850 6892 7271 8065 9200 9881 10072
Size: 452 479 42 379 794 1135 681 191
Cuts at: 10072 826
Size: 1471
Fragments arranged by size:
1584 1471 1135 998 794 681 640 619
552 479 452 379 301 219 191 180
42
SfoI GGC'GCC
Cuts at: 6328 6328
Size: 10717
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10717
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10717
SmaI CCC'GGG
Cuts at: 1872 8044 1872
Size: 6172 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3588 3598 4176 4856 5417 5556
Size: 197 926 1645 10 578 680 561 139
Cuts at: 5556 6300 6518 7416 8821 9722 9984 820
Size: 744 218 898 1405 901 262 1553
Fragments arranged by size:
1645 1553 1405 926 901 898 744 680
578 561 262 218 197 139 10
SnaBI TAC'GTA
Cuts at: 493 8508 493
Size: 8015 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10717
SphI G_CATG'C
Cuts at: 1132 5565 8075 1132
Size: 4433 2510 3774
Fragments arranged by size:
4433 3774 2510
SspI AAT'ATT
Cuts at: 5 52 3730 4101 8641 5
Size: 47 3678 371 4540 2081
Fragments arranged by size:
4540 3678 2081 371 47
StuI AGG'CCT
Cuts at: 1517 2765 6931 7889 1517
Size: 1248 4166 958 4345
Fragments arranged by size:
4345 4166 1248 958
StyI C'CwwG_G
Cuts at: 513 1065 2004 2122 2266 2939 5799 6411 8156
Size: 552 939 118 144 673 2860 612 1745
Cuts at: 8156 513
Size: 3074
Fragments arranged by size:
3074 2860 1745 939 673 612 552 144
118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2874 4745 4986 5807 6398 6481 6548
Size: 1255 1294 1871 241 821 591 83 67
Cuts at: 6548 6815 8744 8761 8914 9099 9544 325
Size: 267 1929 17 153 185 445 1498
Fragments arranged by size:
1929 1871 1498 1294 1255 821 591 445
267 241 185 153 83 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3853 3927 5281
Size: 274 80 33 51 1443 1876 74 1354
Cuts at: 5281 6277 7935 8963 10675 96
Size: 996 1658 1028 1712 138
Fragments arranged by size:
1876 1712 1658 1443 1354 1028 996 274
138 80 74 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4437 4469 5963 5995 7129 7161 10677 10709 4437
Size: 32 1494 32 1134 32 3516 32 4445
Fragments arranged by size:
4445 3516 1494 1134 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5505 1316
Size: 4189 6528
XbaI T'CTAG_A
Cuts at: 8053 8053
Size: 10717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2913 4637 6598 7291 7372 2913
Size: 1724 1961 693 81 6258
Fragments arranged by size:
6258 1961 1724 693 81
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10717
XmaI C'CCGG_G
Cuts at: 1870 8042 1870
Size: 6172 4545
XmnI GAAnn'nnTTC
Cuts at: 3456 6246 8846 3456
Size: 2790 2600 5327
Fragments arranged by size:
5327 2790 2600
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3014 4677 8525 276
Size: 53 83 186 936 1480 1663 3848 2468
Fragments arranged by size:
3848 2468 1663 1480 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BfrBI BsiWI BstZ17I DraIII EcoICRI
FalI FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI RsrII SacI SacII SanDI SfiI SrfI SwaI