Restriction map for
pFN21AB9556
(Circular) (Six-base) MAPSORT of: pFN21AB9556.seq Check: 2541 from: 1 to: 7318
pFN21AB9556 7318 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4426 4426
Size: 7318
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4449 5128 278
Size: 53 83 186 936 2913 679 2468
Fragments arranged by size:
2913 2468 936 679 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3615 4047 4661 1937
Size: 1678 432 614 4594
Fragments arranged by size:
4594 1678 614 432
Acc65I G'GTAC_C
Cuts at: 4639 4639
Size: 7318
AclI AA'CG_TT
Cuts at: 1563 5445 5818 1563
Size: 3882 373 3063
Fragments arranged by size:
3882 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5381 6765 7033 1632
Size: 3749 1384 268 1917
Fragments arranged by size:
3749 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 4126 820
Size: 197 3109 4012
Fragments arranged by size:
4012 3109 197
AflIII A'CryG_T
Cuts at: 1203 4465 4550 6217 7194 1203
Size: 3262 85 1667 977 1327
Fragments arranged by size:
3262 1667 1327 977 85
AgeI A'CCGG_T
Cuts at: 6149 6149
Size: 7318
AhdI GACnn_n'nnGTC
Cuts at: 6047 6047
Size: 7318
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7318
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2631 2663 3005 3037 2631
Size: 32 342 32 6912
Fragments arranged by size:
6912 342 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2092 5061 6633 2092
Size: 2969 1572 2777
Fragments arranged by size:
2969 2777 1572
ApaI G_GGCC'C
Cuts at: 2622 2622
Size: 7318
ApaLI G'TGCA_C
Cuts at: 5375 6531 5375
Size: 1156 6162
ApoI r'AATT_y
Cuts at: 1493 3403 3926 4627 4853 5005 7114 1493
Size: 1910 523 701 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 701 523 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5872 160
Size: 872 4840 1606
Fragments arranged by size:
4840 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 2687 4643 1870
Size: 73 534 210 1956 4545
Fragments arranged by size:
4545 1956 534 210 73
AvrII C'CTAG_G
Cuts at: 2857 2857
Size: 7318
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3380 3413 3380
Size: 33 7285
BamHI G'GATC_C
Cuts at: 4648 5015 4648
Size: 367 6951
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2206 2258 2362 2512 3444
Size: 325 217 627 419 52 104 150 932
Cuts at: 3444 3872 4639 6094 618
Size: 428 767 1455 1842
Fragments arranged by size:
1842 1455 932 767 627 428 419 325
217 150 104 52
BanII G_rGCy'C
Cuts at: 1391 2132 2246 2610 2622 2685 2817 3198 4223
Size: 741 114 364 12 63 132 381 1025
Cuts at: 4223 4318 4637 1391
Size: 95 319 4072
Fragments arranged by size:
4072 1025 741 381 364 319 132 114
95 63 12
BbeI G_GCGC'C
Cuts at: 2262 3876 2262
Size: 1614 5704
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3057 3702 4011 928
Size: 971 1158 645 309 4235
Fragments arranged by size:
4235 1158 971 645 309
BbvCI CC'TCA_GC
Cuts at: 2076 2076
Size: 7318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5509 5543 1903
Size: 34 3572 34 3678
Fragments arranged by size:
3678 3572 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3766 5210 6420 3766
Size: 1444 1210 4664
Fragments arranged by size:
4664 1444 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7318
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5929 136
Size: 107 122 71 631 605 4257 1525
Fragments arranged by size:
4257 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3381 7313 3381
Size: 3932 3386
BlpI GC'TnA_GC
Cuts at: 4410 4735 4410
Size: 325 6993
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2622 5379 6535 1790
Size: 421 411 2757 1156 2573
Fragments arranged by size:
2757 2573 1156 421 411
BmgBI CAC'GTC
Cuts at: 1318 2908 2949 1318
Size: 1590 41 5687
Fragments arranged by size:
5687 1590 41
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2110 2502 5049
Size: 416 53 83 399 24 693 392 2547
Cuts at: 5049 6007 442
Size: 958 1753
Fragments arranged by size:
2547 1753 958 693 416 399 392 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7318
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2195 2195
Size: 7318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4164 4625 5978 1239
Size: 2925 461 1353 2579
Fragments arranged by size:
2925 2579 1353 461
Bpu10I CC'TnA_GC
Cuts at: 2076 2076
Size: 7318
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2816 4006 5443 6308 6606 2816
Size: 1190 1437 865 298 3528
Fragments arranged by size:
3528 1437 1190 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2928 4601 5981 882
Size: 617 1429 1673 1380 2219
Fragments arranged by size:
2219 1673 1429 1380 617
BsaAI yAC'GTr
Cuts at: 493 1204 5109 493
Size: 711 3905 2702
Fragments arranged by size:
3905 2702 711
BsaBI GATnn'nnATC
Cuts at: 7312 7312
Size: 7318
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2259 3873 4446 5125
Size: 53 83 186 936 726 1614 573 679
Cuts at: 5125 5507 6142 275
Size: 382 635 1451
Fragments arranged by size:
1614 1451 936 726 679 635 573 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5751 6149 6423 6570 6981 5751
Size: 398 274 147 411 6088
Fragments arranged by size:
6088 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2334 2364 2631 2637 2661
Size: 30 165 30 931 30 267 6 24
Cuts at: 2661 2667 1178
Size: 6 5829
Fragments arranged by size:
5829 931 267 165 30 30 30 24
6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2310 2495 2675 2819 2900 3946 1186
Size: 1124 185 180 144 81 1046 4558
Fragments arranged by size:
4558 1124 1046 185 180 144 81
BseYI C'CCAG_C
Cuts at: 2262 2386 3024 3918 6521 7017 2262
Size: 124 638 894 2603 496 2563
Fragments arranged by size:
2603 2563 894 638 496 124
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2455 3586 1163
Size: 573 719 1131 4895
Fragments arranged by size:
4895 1131 719 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2069 2581 4768 5529 6557 1540
Size: 275 186 68 512 2187 761 1028 2301
Fragments arranged by size:
2301 2187 1028 761 512 275 186 68
BsiHKAI G_wGCw'C
Cuts at: 2685 4074 4223 5379 5464 6535 2685
Size: 1389 149 1156 85 1071 3468
Fragments arranged by size:
3468 1389 1156 1071 149 85
BsmI GAATG_Cn'
Cuts at: 4829 4922 4829
Size: 93 7225
BsmBI CGTCTCn'nnnn_
Cuts at: 4292 4292
Size: 7318
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2132 2211 2246 2610 2622 2685 2817
Size: 399 342 79 35 364 12 63 132
Cuts at: 2817 3198 4074 4223 4318 4637 5379 5464 6535
Size: 381 876 149 95 319 742 85 1071
Cuts at: 6535 1391
Size: 2174
Fragments arranged by size:
2174 1071 876 742 399 381 364 342
319 149 132 95 85 79 63 35
12
BspEI T'CCGG_A
Cuts at: 6981 6981
Size: 7318
BspHI T'CATG_A
Cuts at: 5207 6937 5207
Size: 1730 5588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4426 4673 844
Size: 3582 247 3489
Fragments arranged by size:
3582 3489 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2273 5205 1394
Size: 429 450 2932 3507
Fragments arranged by size:
3507 2932 450 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3871 5813 5987 65
Size: 1183 2623 1942 174 1396
Fragments arranged by size:
2623 1942 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2428 5962 6149 1700
Size: 110 618 3534 187 2869
Fragments arranged by size:
3534 2869 618 187 110
BsrGI T'GTAC_A
Cuts at: 96 3903 96
Size: 3807 3511
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7318
BssSI C'ACGA_G
Cuts at: 5378 6390 5378
Size: 1012 6306
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7318
BstBI TT'CG_AA
Cuts at: 6122 6122
Size: 7318
BstEII G'GTnAC_C
Cuts at: 1184 2401 3748 1184
Size: 1217 1347 4754
Fragments arranged by size:
4754 1347 1217
BstYI r'GATC_y
Cuts at: 1972 3248 3381 4648 5015 5400 5417 6869 6955
Size: 1276 133 1267 367 385 17 1452 86
Cuts at: 6955 6967 7313 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1276 1267 385 367 346 133
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3795 3795
Size: 7318
BtgI C'CryG_G
Cuts at: 513 1065 2004 2444 2700 513
Size: 552 939 440 256 5131
Fragments arranged by size:
5131 939 552 440 256
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4488 487
Size: 683 56 3262 3317
Fragments arranged by size:
3317 3262 683 56
BtsI GCAGTG_nn'
Cuts at: 2523 2970 4834 5628 5648 2523
Size: 447 1864 794 20 4193
Fragments arranged by size:
4193 1864 794 447 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7318
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7283
DraI TTT'AAA
Cuts at: 3400 3931 4622 3400
Size: 531 691 6096
Fragments arranged by size:
6096 691 531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2871 6325 809
Size: 507 1555 3454 1802
Fragments arranged by size:
3454 1802 1555 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2277 2578 4765
Size: 54 1425 50 275 18 447 301 2187
Cuts at: 4765 5654 7283 8
Size: 889 1629 43
Fragments arranged by size:
2187 1629 1425 889 447 301 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2578 4765 1537
Size: 275 766 2187 4090
Fragments arranged by size:
4090 2187 766 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2756 3180 3964 4085 4158 5248 1353
Size: 1403 424 784 121 73 1090 3423
Fragments arranged by size:
3423 1403 1090 784 424 121 73
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2074 5905 6279 6425 387
Size: 1064 138 485 3831 374 146 1280
Fragments arranged by size:
3831 1280 1064 485 374 146 138
EcoICRI GAG'CTC
Cuts at: 2683 4221 2683
Size: 1538 5780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4164 4625 5381 5978 6765 7033 1239
Size: 393 2532 461 756 597 787 268 1524
Fragments arranged by size:
2532 1524 787 756 597 461 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2562 2562
Size: 7318
EcoO109I rG'GnC_Cy
Cuts at: 2112 2485 2593 2619 2854 2864 3163 2112
Size: 373 108 26 235 10 299 6267
Fragments arranged by size:
6267 373 299 235 108 26 10
EcoRI G'AATT_C
Cuts at: 4627 5005 4627
Size: 378 6940
FspI TGC'GCA
Cuts at: 2840 5824 2840
Size: 2984 4334
HaeII r_GCGC'y
Cuts at: 1678 2262 3876 6465 1678
Size: 584 1614 2589 2531
Fragments arranged by size:
2589 2531 1614 584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3173 3205 3433 3465 4355
Size: 32 135 32 1601 32 228 32 890
Cuts at: 4355 4387 4412 4444 4537 4569 5959 5991 6033
Size: 32 25 32 93 32 1390 32 42
Cuts at: 6033 6065 1373
Size: 32 2626
Fragments arranged by size:
2626 1601 1390 890 228 135 93 42
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 2499 4662 4908 669
Size: 988 281 561 2163 246 3079
Fragments arranged by size:
3079 2163 988 561 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7318
HpaI GTT'AAC
Cuts at: 4908 4908
Size: 7318
KasI G'GCGC_C
Cuts at: 2258 3872 2258
Size: 1614 5704
KpnI G_GTAC'C
Cuts at: 4643 4643
Size: 7318
MfeI C'AATT_G
Cuts at: 4563 4917 4563
Size: 354 6964
MluI A'CGCG_T
Cuts at: 7194 7194
Size: 7318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2820 3486 6432 6616 1305
Size: 1515 666 2946 184 2007
Fragments arranged by size:
2946 2007 1515 666 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2279 10
Size: 54 1425 343 447 5049
Fragments arranged by size:
5049 1425 447 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4266 5276 5635 5794 518
Size: 648 3100 1010 359 159 2042
Fragments arranged by size:
3100 2042 1010 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2266 2446 3922 4320 4347 4734 5411 6559
Size: 660 180 1476 398 27 387 677 1148
Cuts at: 6559 6804 1606
Size: 245 2120
Fragments arranged by size:
2120 1476 1148 677 660 398 387 245
180 27
NarI GG'CG_CC
Cuts at: 2259 3873 2259
Size: 1614 5704
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5827
Fragments arranged by size:
5827 939 552
NdeI CA'TA_TG
Cuts at: 387 3589 387
Size: 3202 4116
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7318
NotI GC'GGCC_GC
Cuts at: 4765 4765
Size: 7318
NspI r_CATG'y
Cuts at: 1132 1229 4554 4676 6221 1132
Size: 97 3325 122 1545 2229
Fragments arranged by size:
3325 2229 1545 122 97
PasI CC'CwG_GG
Cuts at: 1348 2869 2918 4333 1348
Size: 1521 49 1415 4333
Fragments arranged by size:
4333 1521 1415 49
PciI A'CATG_T
Cuts at: 4550 6217 4550
Size: 1667 5651
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2092 2393 1108
Size: 984 301 6033
Fragments arranged by size:
6033 984 301
PmeI GTTT'AAAC
Cuts at: 4622 4622
Size: 7318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2592 2624 2631 2663 5387 5419 6900 6932 2592
Size: 32 7 32 2724 32 1481 32 2978
Fragments arranged by size:
2978 2724 1481 32 32 32 32 7
PpuMI rG'GwC_Cy
Cuts at: 2485 2593 2864 2485
Size: 108 271 6939
Fragments arranged by size:
6939 271 108
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7318
PsiI TTA'TAA
Cuts at: 4888 4888
Size: 7318
PspOMI G'GGCC_C
Cuts at: 2618 2618
Size: 7318
PstI C_TGCA'G
Cuts at: 830 4363 4437 4670 830
Size: 3533 74 233 3478
Fragments arranged by size:
3533 3478 233 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7318
PvuII CAG'CTG
Cuts at: 3922 3922
Size: 7318
SacI G_AGCT'C
Cuts at: 2685 4223 2685
Size: 1538 5780
SacII CC_GC'GG
Cuts at: 2447 2447
Size: 7318
SalI G'TCGA_C
Cuts at: 1936 4660 1936
Size: 2724 4594
SapI GCTCTTCn'nnn_
Cuts at: 4158 4158
Size: 7318
SbfI CC_TGCA'GG
Cuts at: 4437 4670 4437
Size: 233 7085
ScaI AGT'ACT
Cuts at: 5566 7278 5566
Size: 1712 5606
SfcI C'TryA_G
Cuts at: 826 1045 3700 4359 4433 4666 5801 6482 6673
Size: 219 2655 659 74 233 1135 681 191
Cuts at: 6673 826
Size: 1471
Fragments arranged by size:
2655 1471 1135 681 659 233 219 191
74
SfoI GGC'GCC
Cuts at: 2260 3874 2260
Size: 1614 5704
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7318
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7318
SmaI CCC'GGG
Cuts at: 1872 4645 1872
Size: 2773 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 2831 4021 4126 5422 6323
Size: 197 926 744 144 1190 105 1296 901
Cuts at: 6323 6585 820
Size: 262 1553
Fragments arranged by size:
1553 1296 1190 926 901 744 262 197
144 105
SnaBI TAC'GTA
Cuts at: 493 5109 493
Size: 4616 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7318
SphI G_CATG'C
Cuts at: 1132 4676 1132
Size: 3544 3774
SspI AAT'ATT
Cuts at: 5 52 3463 5242 5
Size: 47 3411 1779 2081
Fragments arranged by size:
3411 2081 1779 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7318
StyI C'CwwG_G
Cuts at: 513 1065 2004 2857 4539 4757 513
Size: 552 939 853 1682 218 3074
Fragments arranged by size:
3074 1682 939 853 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2359 4380 5345 5362 5515 5700 6145
Size: 1255 779 2021 965 17 153 185 445
Cuts at: 6145 325
Size: 1498
Fragments arranged by size:
2021 1498 1255 965 779 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2711 3903
Size: 274 80 33 51 1443 327 407 1192
Cuts at: 3903 5564 7276 96
Size: 1661 1712 138
Fragments arranged by size:
1712 1661 1443 1192 407 327 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2334 2366 3557 3589 7278 7310 2334
Size: 32 1191 32 3689 32 2342
Fragments arranged by size:
3689 2342 1191 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4448 1316
Size: 3132 4186
XbaI T'CTAG_A
Cuts at: 4654 4654
Size: 7318
XhoI C'TCGA_G
Cuts at: 1943 2687 1943
Size: 744 6574
XmaI C'CCGG_G
Cuts at: 1870 4643 1870
Size: 2773 4545
XmnI GAAnn'nnTTC
Cuts at: 5447 5447
Size: 7318
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4447 5126 276
Size: 53 83 186 936 2913 679 2468
Fragments arranged by size:
2913 2468 936 679 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI BfrBI BsiWI BstXI BstZ17I DraIII EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspXI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI XcmI