Restriction map for
pFN21AB9555
(Circular) (Six-base) MAPSORT of: pFN21AB9555.seq Check: 9943 from: 1 to: 6043
pFN21AB9555 6043 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2475 2559 3191 2475
Size: 84 632 5327
Fragments arranged by size:
5327 632 84
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3853 278
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2096 2434 2518 2938 3022 4106 5490 5758
Size: 464 338 84 420 84 1084 1384 268
Cuts at: 5758 1632
Size: 1917
Fragments arranged by size:
1917 1384 1084 464 420 338 268 84
84
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 3261 4942 5919 1203
Size: 2058 1681 977 1327
Fragments arranged by size:
2058 1681 1327 977
AgeI A'CCGG_T
Cuts at: 3080 3210 4874 3080
Size: 130 1664 4249
Fragments arranged by size:
4249 1664 130
AhdI GACnn_n'nnGTC
Cuts at: 4772 4772
Size: 6043
AleI CACnn'nnGTG
Cuts at: 1166 3081 1166
Size: 1915 4128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 3786 5358 3786
Size: 1572 4471
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 2200 2736 3352 3578 3730 5839 1493
Size: 707 536 616 226 152 2109 1697
Fragments arranged by size:
2109 1697 707 616 536 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 3368 1870
Size: 73 1425 4545
Fragments arranged by size:
4545 1425 73
BamHI G'GATC_C
Cuts at: 2904 3373 3740 2904
Size: 469 367 5207
Fragments arranged by size:
5207 469 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2559 3207 3364 4819 618
Size: 325 217 627 772 648 157 1455 1842
Fragments arranged by size:
1842 1455 772 648 627 325 217 157
BanII G_rGCy'C
Cuts at: 1391 2274 2340 2462 2546 2630 2714 2798 2966
Size: 883 66 122 84 84 84 84 168
Cuts at: 2966 3050 3299 3362 1391
Size: 84 249 63 4072
Fragments arranged by size:
4072 883 249 168 122 84 84 84
84 84 66 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BbvCI CC'TCA_GC
Cuts at: 2306 2306
Size: 6043
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3290 3324 4234 4268 1903
Size: 34 1353 34 910 34 3678
Fragments arranged by size:
3678 1353 910 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2249 3935 5145 2249
Size: 1686 1210 3147
Fragments arranged by size:
3147 1686 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4654 136
Size: 107 122 71 631 605 2982 1525
Fragments arranged by size:
2982 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 2629 3460 2629
Size: 831 5212
Bme1580I G_kGCm'C
Cuts at: 1790 4104 5260 1790
Size: 2314 1156 2573
Fragments arranged by size:
2573 2314 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 2502 2922
Size: 416 53 83 399 24 917 168 420
Cuts at: 2922 3774 4732 442
Size: 852 958 1753
Fragments arranged by size:
1753 958 917 852 420 416 399 168
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2276 2698 4703 1239
Size: 1037 422 2005 2579
Fragments arranged by size:
2579 2005 1037 422
Bpu10I CC'TnA_GC
Cuts at: 2306 2555 2797 2804 2306
Size: 249 242 7 5545
Fragments arranged by size:
5545 249 242 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2200 4168 5033 5331 2200
Size: 1968 865 298 2912
Fragments arranged by size:
2912 1968 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2746 4706 882
Size: 617 531 716 1960 2219
Fragments arranged by size:
2219 1960 716 617 531
BsaAI yAC'GTr
Cuts at: 493 1204 3262 3834 493
Size: 711 2058 572 2702
Fragments arranged by size:
2702 2058 711 572
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3850 4232 4867 275
Size: 53 83 186 936 2317 382 635 1451
Fragments arranged by size:
2317 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2660 3080 3210 4476 4874 5148 5295 5706 2660
Size: 420 130 1266 398 274 147 411 2997
Fragments arranged by size:
2997 1266 420 411 398 274 147 130
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5818
Fragments arranged by size:
5818 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3040 1186
Size: 1854 4189
BseYI C'CCAG_C
Cuts at: 2017 5246 5742 2017
Size: 3229 496 2318
Fragments arranged by size:
3229 2318 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2376 2701 1163
Size: 573 640 325 4505
Fragments arranged by size:
4505 640 573 325
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3493 4254 5282 1540
Size: 275 186 1492 761 1028 2301
Fragments arranged by size:
2301 1492 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2216 2274 2462 2546 2630 2714 2798 2966 2982
Size: 58 188 84 84 84 84 168 16
Cuts at: 2982 3050 3066 3231 3299 4104 4189 5260 2216
Size: 68 16 165 68 805 85 1071 2999
Fragments arranged by size:
2999 1071 805 188 168 165 85 84
84 84 84 68 68 58 16 16
BsmI GAATG_Cn'
Cuts at: 2693 3554 3647 2693
Size: 861 93 5089
Fragments arranged by size:
5089 861 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2216 2274 2340 2462 2546 2630 2714
Size: 399 426 58 66 122 84 84 84
Cuts at: 2714 2798 2966 2982 3050 3066 3231 3299 3362
Size: 84 168 16 68 16 165 68 63
Cuts at: 3362 4104 4189 5260 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 426 399 168 165 122
85 84 84 84 84 68 68 66
63 58 16 16
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 2559 3191 3398 844
Size: 1631 84 632 207 3489
Fragments arranged by size:
3489 1631 632 207 84
BsrBI CCG'CTC
Cuts at: 1394 1823 3930 1394
Size: 429 2107 3507
Fragments arranged by size:
3507 2107 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3080 3164 3210 4687 4874 1700
Size: 110 1270 84 46 1477 187 2869
Fragments arranged by size:
2869 1477 1270 187 110 84 46
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6043
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6043
BssSI C'ACGA_G
Cuts at: 2729 2843 4103 5115 2729
Size: 114 1260 1012 3657
Fragments arranged by size:
3657 1260 1012 114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2688 3273 794
Size: 1894 585 3564
Fragments arranged by size:
3564 1894 585
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstXI CCAn_nnnn'nTGG
Cuts at: 3026 3026
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 2154 2652 2820 2904 3373 3740 4125 4142
Size: 182 498 168 84 469 367 385 17
Cuts at: 4142 5594 5680 5692 6038 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 498 469 385 367 346 182
168 86 84 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2949 487
Size: 683 56 1723 3581
Fragments arranged by size:
3581 1723 683 56
BtsI GCAGTG_nn'
Cuts at: 3559 4353 4373 3559
Size: 794 20 5229
Fragments arranged by size:
5229 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DraIII CAC_nnn'GTG
Cuts at: 2482 2482
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3490 4379 6008
Size: 54 1425 50 275 18 1660 889 1629
Cuts at: 6008 8
Size: 43
Fragments arranged by size:
1660 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3490 1537
Size: 275 1678 4090
Fragments arranged by size:
4090 1678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 2328 3973 1353
Size: 926 49 1645 3423
Fragments arranged by size:
3423 1645 926 49
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2609 4630 5004 5150 387
Size: 1064 138 1020 2021 374 146 1280
Fragments arranged by size:
2021 1280 1064 1020 374 146 138
EcoICRI GAG'CTC
Cuts at: 2272 2460 2544 2628 2712 2796 2964 3048 3297
Size: 188 84 84 84 84 168 84 249
Cuts at: 3297 2272
Size: 5018
Fragments arranged by size:
5018 249 188 168 84 84 84 84
84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2276 2434 2518 2698 2938 3022
Size: 393 464 180 158 84 180 240 84
Cuts at: 3022 4106 4703 5490 5758 1239
Size: 1084 597 787 268 1524
Fragments arranged by size:
1524 1084 787 597 464 393 268 240
180 180 158 84 84
EcoO109I rG'GnC_Cy
Cuts at: 2092 2101 2381 2670 2092
Size: 9 280 289 5465
Fragments arranged by size:
5465 289 280 9
EcoRI G'AATT_C
Cuts at: 2736 3352 3730 2736
Size: 616 378 5049
Fragments arranged by size:
5049 616 378
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 5190 1678
Size: 3512 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2743 2775 2797 2829 3298
Size: 32 135 32 1171 32 22 32 469
Cuts at: 3298 3330 4684 4716 4758 4790 1373
Size: 32 1354 32 42 32 2626
Fragments arranged by size:
2626 1354 1171 469 135 42 32 32
32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2325 2497 2917 2991 5157 5341 1305
Size: 1020 172 420 74 2166 184 2007
Fragments arranged by size:
2166 2007 1020 420 184 172 74
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4221
Fragments arranged by size:
4221 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2929 3081 4001 4360 4519 518
Size: 648 1763 152 920 359 159 2042
Fragments arranged by size:
2042 1763 920 648 359 159 152
MspA1I CmG'CkG
Cuts at: 1606 2902 3459 4136 5284 5529 1606
Size: 1296 557 677 1148 245 2120
Fragments arranged by size:
2120 1296 1148 677 557 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4552
Fragments arranged by size:
4552 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 2314 3401 4946 1132
Size: 97 1085 1087 1545 2229
Fragments arranged by size:
2229 1545 1087 1085 97
PasI CC'CwG_GG
Cuts at: 1348 2084 1348
Size: 736 5307
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6043
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2092 2101 2381 2092
Size: 9 280 5754
Fragments arranged by size:
5754 280 9
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6043
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PstI C_TGCA'G
Cuts at: 830 3395 830
Size: 2565 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
SacI G_AGCT'C
Cuts at: 2274 2462 2546 2630 2714 2798 2966 3050 3299
Size: 188 84 84 84 84 168 84 249
Cuts at: 3299 2274
Size: 5018
Fragments arranged by size:
5018 249 188 168 84 84 84 84
84
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SanDI GG'GwC_CC
Cuts at: 2101 2101
Size: 6043
SapI GCTCTTCn'nnn_
Cuts at: 2279 2328 2279
Size: 49 5994
SbfI CC_TGCA'GG
Cuts at: 3395 3395
Size: 6043
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SexAI A'CCwGG_T
Cuts at: 2478 2478
Size: 6043
SfcI C'TryA_G
Cuts at: 826 1045 3391 4526 5207 5398 826
Size: 219 2346 1135 681 191 1471
Fragments arranged by size:
2346 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 3080 1700
Size: 1380 4663
SmaI CCC'GGG
Cuts at: 1872 3370 1872
Size: 1498 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2215 4147 5048 5310 820
Size: 197 926 272 1932 901 262 1553
Fragments arranged by size:
1932 1553 926 901 272 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2447 2531 2615 2802 2951 3119 1517
Size: 930 84 84 187 149 168 4441
Fragments arranged by size:
4441 930 187 168 149 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2004 3482 513
Size: 552 939 1478 3074
Fragments arranged by size:
3074 1478 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4070 4087 4240 4425 4870 325
Size: 1255 2490 17 153 185 445 1498
Fragments arranged by size:
2490 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4289 6001 96
Size: 274 80 33 51 1443 2312 1712 138
Fragments arranged by size:
2312 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2874 2906 6003 6035 2874
Size: 32 3097 32 2882
Fragments arranged by size:
3097 2882 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 3368 1870
Size: 1498 4545
XmnI GAAnn'nnTTC
Cuts at: 2447 2531 2615 2699 2783 2951 3035 4172 2447
Size: 84 84 84 84 168 84 1137 4318
Fragments arranged by size:
4318 1137 168 84 84 84 84 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3851 276
Size: 53 83 186 936 2317 2468
Fragments arranged by size:
2468 2317 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SfiI SfoI SrfI SwaI XcmI