Restriction map for
pFN21AB9552
(Circular) (Six-base) MAPSORT of: pFN21AB9552.seq Check: 381 from: 1 to: 6103
pFN21AB9552 6103 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2397 2397
Size: 6103
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3446 1937
Size: 1509 4594
Acc65I G'GTAC_C
Cuts at: 3424 3424
Size: 6103
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4166 5550 5818 1632
Size: 2534 1384 268 1917
Fragments arranged by size:
2534 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 5002 5979 1203
Size: 3799 977 1327
Fragments arranged by size:
3799 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 2905 3846 5418 2905
Size: 941 1572 3590
Fragments arranged by size:
3590 1572 941
ApaI G_GGCC'C
Cuts at: 2103 3093 3102 3294 3345 2103
Size: 990 9 192 51 4861
Fragments arranged by size:
4861 990 192 51 9
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 3412 3638 3790 5899 1493
Size: 1919 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4657 160
Size: 872 3625 1606
Fragments arranged by size:
3625 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 2077 2140 2687 3247 3428 1870
Size: 73 134 63 547 560 181 4545
Fragments arranged by size:
4545 560 547 181 134 73 63
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 2291 2649 2919 3424
Size: 325 217 627 479 25 358 270 505
Cuts at: 3424 4879 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 505 479 358 325 270
217 25
BanII G_rGCy'C
Cuts at: 1391 2045 2054 2064 2103 2350 2406 2666 3093
Size: 654 9 10 39 247 56 260 427
Cuts at: 3093 3102 3294 3345 3422 1391
Size: 9 192 51 77 4072
Fragments arranged by size:
4072 654 427 260 247 192 77 56
51 39 10 9 9
BbeI G_GCGC'C
Cuts at: 2295 2295
Size: 6103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2747 3080 928
Size: 971 848 333 3951
Fragments arranged by size:
3951 971 848 333
BbvCI CC'TCA_GC
Cuts at: 3152 3152
Size: 6103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2350 2384 4294 4328 1903
Size: 34 413 34 1910 34 3678
Fragments arranged by size:
3678 1910 413 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2049 2533 2615
Size: 107 122 71 631 605 377 484 82
Cuts at: 2615 2917 3097 3249 4714 136
Size: 302 180 152 1465 1525
Fragments arranged by size:
1525 1465 631 605 484 377 302 180
152 122 107 82 71
BglII A'GATC_T
Cuts at: 6098 6098
Size: 6103
BlpI GC'TnA_GC
Cuts at: 2039 2243 2400 2405 2475 3172 3520 2039
Size: 204 157 5 70 697 348 4622
Fragments arranged by size:
4622 697 348 204 157 70 5
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2271 2576 2922 2974 3093 3102 3294
Size: 313 168 305 346 52 119 9 192
Cuts at: 3294 3345 4164 5320 1790
Size: 51 819 1156 2573
Fragments arranged by size:
2573 1156 819 346 313 305 192 168
119 52 51 9
BmgBI CAC'GTC
Cuts at: 1318 2314 1318
Size: 996 5107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3193 3340 3376
Size: 416 53 83 399 24 1776 147 36
Cuts at: 3376 3834 4792 442
Size: 458 958 1753
Fragments arranged by size:
1776 1753 958 458 416 399 147 83
53 36 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6103
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2713 2713
Size: 6103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2449 4763 1239
Size: 1210 2314 2579
Fragments arranged by size:
2579 2314 1210
Bpu10I CC'TnA_GC
Cuts at: 2349 3152 2349
Size: 803 5300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4228 5093 5391 4228
Size: 865 298 4940
Fragments arranged by size:
4940 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2867 3043 3074 4766 882
Size: 617 1368 176 31 1692 2219
Fragments arranged by size:
2219 1692 1368 617 176 31
BsaAI yAC'GTr
Cuts at: 493 1204 3894 493
Size: 711 2690 2702
Fragments arranged by size:
2702 2690 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2292 3910 4292 4927
Size: 53 83 186 936 759 1618 382 635
Cuts at: 4927 275
Size: 1451
Fragments arranged by size:
1618 1451 936 759 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4536 4934 5208 5355 5766 4536
Size: 398 274 147 411 4873
Fragments arranged by size:
4873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 1178
Size: 30 165 30 1368 30 4480
Fragments arranged by size:
4480 1368 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2027 2072 2483 1186
Size: 841 45 411 4806
Fragments arranged by size:
4806 841 411 45
BseYI C'CCAG_C
Cuts at: 2155 2416 2962 3329 5306 5802 2155
Size: 261 546 367 1977 496 2456
Fragments arranged by size:
2456 1977 546 496 367 261
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2375 1163
Size: 573 639 4891
Fragments arranged by size:
4891 639 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3553 4314 5342 1540
Size: 275 186 1552 761 1028 2301
Fragments arranged by size:
2301 1552 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2244 2406 2694 4164 4249 5320 2244
Size: 162 288 1470 85 1071 3027
Fragments arranged by size:
3027 1470 1071 288 162 85
BsmI GAATG_Cn'
Cuts at: 3614 3707 3614
Size: 93 6010
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2054 2064 2103 2244 2271 2350
Size: 399 255 9 10 39 141 27 79
Cuts at: 2350 2406 2576 2666 2694 2922 2974 3093 3102
Size: 56 170 90 28 228 52 119 9
Cuts at: 3102 3294 3345 3422 4164 4249 5320 1391
Size: 192 51 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 255 228 192 170
141 119 90 85 79 77 56 52
51 39 28 27 10 9 9
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 3992 5722 3992
Size: 1730 4373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2397 2913 3458 844
Size: 1553 516 545 3489
Fragments arranged by size:
3489 1553 545 516
BsrBI CCG'CTC
Cuts at: 1394 1823 2479 3990 1394
Size: 429 656 1511 3507
Fragments arranged by size:
3507 1511 656 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4598 4772 65
Size: 1183 3350 174 1396
Fragments arranged by size:
3350 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2646 2951 4747 4934 1700
Size: 110 836 305 1796 187 2869
Fragments arranged by size:
2869 1796 836 305 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6103
BssHII G'CGCG_C
Cuts at: 1925 2085 2357 1925
Size: 160 272 5671
Fragments arranged by size:
5671 272 160
BssSI C'ACGA_G
Cuts at: 2852 4163 5175 2852
Size: 1311 1012 3780
Fragments arranged by size:
3780 1311 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6103
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstXI CCAn_nnnn'nTGG
Cuts at: 2711 3260 2711
Size: 549 5554
BstYI r'GATC_y
Cuts at: 1972 2020 3003 3433 3800 4185 4202 5654 5740
Size: 48 983 430 367 385 17 1452 86
Cuts at: 5740 5752 6098 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 983 430 385 367 346 86
48 17 12
Bsu36I CC'TnA_GG
Cuts at: 2698 3226 3337 2698
Size: 528 111 5464
Fragments arranged by size:
5464 528 111
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 2710 2790 2979 3033 3084
Size: 552 939 42 664 80 189 54 51
Cuts at: 3084 513
Size: 3532
Fragments arranged by size:
3532 939 664 552 189 80 54 51
42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 2974 3619 4413 4433 2974
Size: 645 794 20 4644
Fragments arranged by size:
4644 794 645 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DraIII CAC_nnn'GTG
Cuts at: 3050 3050
Size: 6103
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2976 2982 3241
Size: 54 1425 50 275 18 1146 6 259
Cuts at: 3241 3315 3550 4439 6068 8
Size: 74 235 889 1629 43
Fragments arranged by size:
1629 1425 1146 889 275 259 235 74
54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4033 1353
Size: 2680 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2133 2207 2551 2582 2986
Size: 1064 138 435 109 74 344 31 404
Cuts at: 2986 4690 5064 5210 387
Size: 1704 374 146 1280
Fragments arranged by size:
1704 1280 1064 435 404 374 344 146
138 109 74 31
EcoICRI GAG'CTC
Cuts at: 2404 2404
Size: 6103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2449 4166 4763 5550 5818 1239
Size: 393 817 1717 597 787 268 1524
Fragments arranged by size:
1717 1524 817 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2099 3089 3090 3098 3099 3230 3290 3291 3341
Size: 990 1 8 1 131 60 1 50
Cuts at: 3341 3342 2099
Size: 1 4860
Fragments arranged by size:
4860 990 131 60 50 8 1 1
1 1
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2476 2476
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 2295 2458 2634 2788 5250 1678
Size: 617 163 176 154 2462 2531
Fragments arranged by size:
2531 2462 617 176 163 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2738 2770 2771 2803 2846
Size: 32 135 32 1166 32 1 32 43
Cuts at: 2846 2878 3107 3139 4744 4776 4818 4850 1373
Size: 32 229 32 1605 32 42 32 2626
Fragments arranged by size:
2626 1605 1166 229 135 43 42 32
32 32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2287 3447 3693 669
Size: 988 281 349 1160 246 3079
Fragments arranged by size:
3079 1160 988 349 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KasI G'GCGC_C
Cuts at: 2291 2291
Size: 6103
KpnI G_GTAC'C
Cuts at: 3428 3428
Size: 6103
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2753 2874 2889 5217 5401 1305
Size: 1448 121 15 2328 184 2007
Fragments arranged by size:
2328 2007 1448 184 121 15
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2984 3243 10
Size: 54 1425 343 1152 259 2870
Fragments arranged by size:
2870 1425 1152 343 259 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2276 4061 4420 4579 518
Size: 648 1110 1785 359 159 2042
Fragments arranged by size:
2042 1785 1110 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 2247 3086 3301 3519 4196 5344 5589
Size: 461 180 839 215 218 677 1148 245
Cuts at: 5589 1606
Size: 2120
Fragments arranged by size:
2120 1148 839 677 461 245 218 215
180
NaeI GCC'GGC
Cuts at: 2953 2953
Size: 6103
NarI GG'CG_CC
Cuts at: 2292 2292
Size: 6103
NcoI C'CATG_G
Cuts at: 513 1065 2004 2710 2790 513
Size: 552 939 706 80 3826
Fragments arranged by size:
3826 939 706 552 80
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NgoMIV G'CCGG_C
Cuts at: 2951 2951
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6103
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 3461 5006 1132
Size: 97 2232 1545 2229
Fragments arranged by size:
2232 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2557 3094 1348
Size: 1209 537 4357
Fragments arranged by size:
4357 1209 537
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3366 1108
Size: 2258 3845
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3230 3230
Size: 6103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PspOMI G'GGCC_C
Cuts at: 2099 3089 3098 3290 3341 2099
Size: 990 9 192 51 4861
Fragments arranged by size:
4861 990 192 51 9
PstI C_TGCA'G
Cuts at: 830 2754 3455 830
Size: 1924 701 3478
Fragments arranged by size:
3478 1924 701
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
SacI G_AGCT'C
Cuts at: 2406 2406
Size: 6103
SacII CC_GC'GG
Cuts at: 3087 3087
Size: 6103
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 4351 6063 4351
Size: 1712 4391
SexAI A'CCwGG_T
Cuts at: 3046 3046
Size: 6103
SfcI C'TryA_G
Cuts at: 826 1045 2750 3451 4586 5267 5458 826
Size: 219 1705 701 1135 681 191 1471
Fragments arranged by size:
1705 1471 1135 701 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3097 3249 3097
Size: 152 5951
SfoI GGC'GCC
Cuts at: 2293 2293
Size: 6103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 2079 2142 3430 1872
Size: 207 63 1288 4545
Fragments arranged by size:
4545 1288 207 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4207 5108 5370 820
Size: 197 926 2264 901 262 1553
Fragments arranged by size:
2264 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 3257 1517
Size: 1740 4363
StyI C'CwwG_G
Cuts at: 513 1065 2004 2710 2790 3542 513
Size: 552 939 706 80 752 3074
Fragments arranged by size:
3074 939 752 706 552 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2667 2734 2937 4130 4147 4300 4485
Size: 1255 1087 67 203 1193 17 153 185
Cuts at: 4485 4930 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1193 1087 445 203 185 153
67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4349 6061 96
Size: 274 80 33 51 1443 2372 1712 138
Fragments arranged by size:
2372 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6063 6095 6063
Size: 32 6071
Tth111I GACn'n_nGTC
Cuts at: 1316 3391 1316
Size: 2075 4028
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 2077 2140 3428 1870
Size: 207 63 1288 4545
Fragments arranged by size:
4545 1288 207 63
XmnI GAAnn'nnTTC
Cuts at: 2333 4232 2333
Size: 1899 4204
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoNI EcoRV FseI FspAI NruI NsiI PacI
PfoI PmlI PspXI PsrI PvuII RsrII SanDI SapI
SrfI SwaI XcmI