Restriction map for
pFN21AB9551
(Circular) (Six-base) MAPSORT of: pFN21AB9551.seq Check: 2671 from: 1 to: 5860
pFN21AB9551 5860 bp
With 182 enzymes: SgfI *
February 27, 2009 14:44 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3670 278
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3203 1937
Size: 1266 4594
Acc65I G'GTAC_C
Cuts at: 2398 3181 2398
Size: 783 5077
AclI AA'CG_TT
Cuts at: 1563 3987 4360 1563
Size: 2424 373 3063
Fragments arranged by size:
3063 2424 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2909 2971 3923 5307 5575 1632
Size: 1277 62 952 1384 268 1917
Fragments arranged by size:
1917 1384 1277 952 268 62
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5663
AflIII A'CryG_T
Cuts at: 1203 2850 4759 5736 1203
Size: 1647 1909 977 1327
Fragments arranged by size:
1909 1647 1327 977
AgeI A'CCGG_T
Cuts at: 4691 4691
Size: 5860
AhdI GACnn_n'nnGTC
Cuts at: 4589 4589
Size: 5860
AleI CACnn'nnGTG
Cuts at: 1166 2264 1166
Size: 1098 4762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5860
AlwNI CAG_nnn'CTG
Cuts at: 2602 3080 3603 5175 2602
Size: 478 523 1572 3287
Fragments arranged by size:
3287 1572 523 478
ApaLI G'TGCA_C
Cuts at: 3917 5073 3917
Size: 1156 4704
ApoI r'AATT_y
Cuts at: 1493 3169 3395 3547 5656 1493
Size: 1676 226 152 2109 1697
Fragments arranged by size:
2109 1697 1676 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4414 160
Size: 872 3382 1606
Fragments arranged by size:
3382 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
AvaI C'yCGr_G
Cuts at: 1870 1943 2552 3185 1870
Size: 73 609 633 4545
Fragments arranged by size:
4545 633 609 73
BamHI G'GATC_C
Cuts at: 3190 3557 3190
Size: 367 5493
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2398 3051 3181 4636 618
Size: 325 217 627 611 653 130 1455 1842
Fragments arranged by size:
1842 1455 653 627 611 325 217 130
BanII G_rGCy'C
Cuts at: 1391 2352 3179 1391
Size: 961 827 4072
Fragments arranged by size:
4072 961 827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2981 928
Size: 971 1082 3807
Fragments arranged by size:
3807 1082 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2014 2048 4051 4085 1903
Size: 34 77 34 2003 34 3678
Fragments arranged by size:
3678 2003 77 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2237 3752 4962 2237
Size: 1515 1210 3135
Fragments arranged by size:
3135 1515 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5860
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3146 4471 136
Size: 107 122 71 631 605 1474 1325 1525
Fragments arranged by size:
1525 1474 1325 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5855 5855
Size: 5860
BlpI GC'TnA_GC
Cuts at: 2021 2960 3277 2021
Size: 939 317 4604
Fragments arranged by size:
4604 939 317
Bme1580I G_kGCm'C
Cuts at: 1790 3056 3921 5077 1790
Size: 1266 865 1156 2573
Fragments arranged by size:
2573 1266 1156 865
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5860
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3591 4549 442
Size: 416 53 83 399 24 2174 958 1753
Fragments arranged by size:
2174 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2176 2786 3083 4520 1239
Size: 842 95 610 297 1437 2579
Fragments arranged by size:
2579 1437 842 610 297 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3985 4850 5148 3985
Size: 865 298 4697
Fragments arranged by size:
4697 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4523 882
Size: 617 3024 2219
Fragments arranged by size:
3024 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3651 493
Size: 711 2447 2702
Fragments arranged by size:
2702 2447 711
BsaBI GATnn'nnATC
Cuts at: 3020 5854 3020
Size: 2834 3026
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2610 2645 3133 3667
Size: 53 83 186 936 1077 35 488 534
Cuts at: 3667 4049 4684 275
Size: 382 635 1451
Fragments arranged by size:
1451 1077 936 635 534 488 382 186
83 53 35
BsaWI w'CCGG_w
Cuts at: 3024 4293 4691 4965 5112 5523 3024
Size: 1269 398 274 147 411 3361
Fragments arranged by size:
3361 1269 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2759 2789 2894 2924 1178
Size: 30 165 30 1356 30 105 30 4114
Fragments arranged by size:
4114 1356 165 105 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5860
BseYI C'CCAG_C
Cuts at: 2521 2905 5063 5559 2521
Size: 384 2158 496 2822
Fragments arranged by size:
2822 2158 496 384
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5287
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3310 4071 5099 1540
Size: 275 186 1309 761 1028 2301
Fragments arranged by size:
2301 1309 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2900 3921 4006 5077 2900
Size: 1021 85 1071 3683
Fragments arranged by size:
3683 1071 1021 85
BsmI GAATG_Cn'
Cuts at: 3371 3464 3371
Size: 93 5767
BsmBI CGTCTCn'nnnn_
Cuts at: 2601 2601
Size: 5860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2352 2900 3056 3179 3921 4006 5077
Size: 399 562 548 156 123 742 85 1071
Cuts at: 5077 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 562 548 399 156 123
85
BspEI T'CCGG_A
Cuts at: 3024 5523 3024
Size: 2499 3361
BspHI T'CATG_A
Cuts at: 3749 5479 3749
Size: 1730 4130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3215 844
Size: 2371 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3747 1394
Size: 429 1924 3507
Fragments arranged by size:
3507 1924 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4355 4529 65
Size: 1183 3107 174 1396
Fragments arranged by size:
3107 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4504 4691 1700
Size: 110 2694 187 2869
Fragments arranged by size:
2869 2694 187 110
BsrGI T'GTAC_A
Cuts at: 96 2133 96
Size: 2037 3823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5860
BssSI C'ACGA_G
Cuts at: 3118 3920 4932 3118
Size: 802 1012 4046
Fragments arranged by size:
4046 1012 802
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2602 794
Size: 1808 4052
BstBI TT'CG_AA
Cuts at: 4664 4664
Size: 5860
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5860
BstXI CCAn_nnnn'nTGG
Cuts at: 2575 2575
Size: 5860
BstYI r'GATC_y
Cuts at: 1972 3096 3190 3557 3942 3959 5411 5497 5509
Size: 1124 94 367 385 17 1452 86 12
Cuts at: 5509 5855 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1124 385 367 346 94 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2363 2363
Size: 5860
BtgI C'CryG_G
Cuts at: 513 1065 2751 513
Size: 552 1686 3622
Fragments arranged by size:
3622 1686 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2698 2920 3051 487
Size: 683 56 1472 222 131 3296
Fragments arranged by size:
3296 1472 683 222 131 56
BtsI GCAGTG_nn'
Cuts at: 3056 3376 4170 4190 3056
Size: 320 794 20 4726
Fragments arranged by size:
4726 794 320 20
ClaI AT'CG_AT
Cuts at: 1554 2589 1554
Size: 1035 4825
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5825
DraI TTT'AAA
Cuts at: 3164 3164
Size: 5860
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2786 4867 809
Size: 507 1470 2081 1802
Fragments arranged by size:
2081 1802 1470 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3307 4196 5825
Size: 54 1425 50 275 18 1477 889 1629
Cuts at: 5825 8
Size: 43
Fragments arranged by size:
1629 1477 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3307 1537
Size: 275 1495 4090
Fragments arranged by size:
4090 1495 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2172 2289 2480 3790 1353
Size: 819 117 191 1310 3423
Fragments arranged by size:
3423 1310 819 191 117
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3139 4447 4821 4967 387
Size: 1064 138 1550 1308 374 146 1280
Fragments arranged by size:
1550 1308 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2176 2786 2909 2971 3083 3923
Size: 393 449 95 610 123 62 112 840
Cuts at: 3923 4520 5307 5575 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 840 787 610 597 449 393 268
123 112 95 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2362 2362
Size: 5860
EcoRI G'AATT_C
Cuts at: 3169 3547 3169
Size: 378 5482
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2509 2509
Size: 5860
FspI TGC'GCA
Cuts at: 4366 4366
Size: 5860
HaeII r_GCGC'y
Cuts at: 1678 2021 5007 1678
Size: 343 2986 2531
Fragments arranged by size:
2986 2531 343
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2528 2560 2721 2753 2759
Size: 32 135 32 956 32 161 32 6
Cuts at: 2759 2791 2953 2985 3118 3150 4501 4533 4575
Size: 32 162 32 133 32 1351 32 42
Cuts at: 4575 4607 1373
Size: 32 2626
Fragments arranged by size:
2626 1351 956 162 161 135 133 42
32 32 32 32 32 32 32 32
32 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3450 669
Size: 988 281 1266 246 3079
Fragments arranged by size:
3079 1266 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5860
HpaI GTT'AAC
Cuts at: 3450 3450
Size: 5860
KpnI G_GTAC'C
Cuts at: 2402 3185 2402
Size: 783 5077
MfeI C'AATT_G
Cuts at: 3459 3459
Size: 5860
MluI A'CGCG_T
Cuts at: 5736 5736
Size: 5860
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4974 5158 1305
Size: 3669 184 2007
Fragments arranged by size:
3669 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4038
Fragments arranged by size:
4038 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2264 2430 2810 3818 4177 4336
Size: 648 843 255 166 380 1008 359 159
Cuts at: 4336 518
Size: 2042
Fragments arranged by size:
2042 1008 843 648 380 359 255 166
159
MspA1I CmG'CkG
Cuts at: 1606 2059 2525 2695 2959 3276 3953 5101 5346
Size: 453 466 170 264 317 677 1148 245
Cuts at: 5346 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 466 453 317 264 245
170
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5308
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5860
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5860
NotI GC'GGCC_GC
Cuts at: 3307 3307
Size: 5860
NspI r_CATG'y
Cuts at: 1132 1229 2854 3218 4763 1132
Size: 97 1625 364 1545 2229
Fragments arranged by size:
2229 1625 1545 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5860
PciI A'CATG_T
Cuts at: 2850 4759 2850
Size: 1909 3951
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5860
PfoI T'CCnGG_A
Cuts at: 2763 2763
Size: 5860
PmeI GTTT'AAAC
Cuts at: 3164 3164
Size: 5860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3929 3961 5442 5474 3929
Size: 32 1481 32 4315
Fragments arranged by size:
4315 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5860
PsiI TTA'TAA
Cuts at: 3430 3430
Size: 5860
PstI C_TGCA'G
Cuts at: 830 3212 830
Size: 2382 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5860
PvuII CAG'CTG
Cuts at: 2059 2525 2959 2059
Size: 466 434 4960
Fragments arranged by size:
4960 466 434
SalI G'TCGA_C
Cuts at: 1936 3202 1936
Size: 1266 4594
SbfI CC_TGCA'GG
Cuts at: 3212 3212
Size: 5860
ScaI AGT'ACT
Cuts at: 4108 5820 4108
Size: 1712 4148
SfcI C'TryA_G
Cuts at: 826 1045 3208 4343 5024 5215 826
Size: 219 2163 1135 681 191 1471
Fragments arranged by size:
2163 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5860
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5860
SmaI CCC'GGG
Cuts at: 1872 3187 1872
Size: 1315 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3964 4865 5127 820
Size: 197 926 2021 901 262 1553
Fragments arranged by size:
2021 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3651 493
Size: 3158 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5860
SphI G_CATG'C
Cuts at: 1132 3218 1132
Size: 2086 3774
SspI AAT'ATT
Cuts at: 5 52 3784 5
Size: 47 3732 2081
Fragments arranged by size:
3732 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5860
StyI C'CwwG_G
Cuts at: 513 1065 3299 513
Size: 552 2234 3074
Fragments arranged by size:
3074 2234 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3887 3904 4057 4242 4687 325
Size: 1255 2307 17 153 185 445 1498
Fragments arranged by size:
2307 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2133 2322 4106
Size: 274 80 33 51 1443 156 189 1784
Cuts at: 4106 5818 96
Size: 1712 138
Fragments arranged by size:
1784 1712 1443 274 189 156 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3035 3067 5820 5852 3035
Size: 32 2753 32 3043
Fragments arranged by size:
3043 2753 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5860
XbaI T'CTAG_A
Cuts at: 3196 3196
Size: 5860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5860
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5860
XmaI C'CCGG_G
Cuts at: 1870 3185 1870
Size: 1315 4545
XmnI GAAnn'nnTTC
Cuts at: 2298 2821 3989 2298
Size: 523 1168 4169
Fragments arranged by size:
4169 1168 523
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3668 276
Size: 53 83 186 936 2134 2468
Fragments arranged by size:
2468 2134 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI