Restriction map for
pFN21AB9546
(Circular) (Six-base) MAPSORT of: pFN21AB9546.seq Check: 7025 from: 1 to: 6172
pFN21AB9546 6172 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2993 2993
Size: 6172
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3515 1937
Size: 1578 4594
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 3471 4299 4672 1563
Size: 1908 828 373 3063
Fragments arranged by size:
3063 1908 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4235 5619 5887 1632
Size: 2603 1384 268 1917
Fragments arranged by size:
2603 1917 1384 268
AfeI AGC'GCT
Cuts at: 2730 2730
Size: 6172
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 5071 6048 1203
Size: 3868 977 1327
Fragments arranged by size:
3868 1327 977
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 2144 2507 1166
Size: 978 363 4831
Fragments arranged by size:
4831 978 363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3212 1184
Size: 2028 4144
AlwNI CAG_nnn'CTG
Cuts at: 2150 3175 3217 3915 5487 2150
Size: 1025 42 698 1572 2835
Fragments arranged by size:
2835 1572 1025 698 42
ApaI G_GGCC'C
Cuts at: 3331 3411 3331
Size: 80 6092
ApaLI G'TGCA_C
Cuts at: 3125 4229 5385 3125
Size: 1104 1156 3912
Fragments arranged by size:
3912 1156 1104
ApoI r'AATT_y
Cuts at: 1493 3481 3707 3859 5968 1493
Size: 1988 226 152 2109 1697
Fragments arranged by size:
2109 1988 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2260 2603 2705 2989 3497 1870
Size: 73 81 236 343 102 284 508 4545
Fragments arranged by size:
4545 508 343 284 236 102 81 73
BamHI G'GATC_C
Cuts at: 3502 3869 3502
Size: 367 5805
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2981 3034 3063 3299 3493
Size: 325 217 627 1194 53 29 236 194
Cuts at: 3493 4948 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1194 627 325 236 217 194
53 29
BanII G_rGCy'C
Cuts at: 1391 2412 2656 3331 3411 3491 1391
Size: 1021 244 675 80 80 4072
Fragments arranged by size:
4072 1021 675 244 80 80
BbeI G_GCGC'C
Cuts at: 3038 3038
Size: 6172
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2685 3278 928
Size: 971 786 593 3822
Fragments arranged by size:
3822 971 786 593
BbvCI CC'TCA_GC
Cuts at: 3005 3005
Size: 6172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 1903
Size: 34 2426 34 3678
Fragments arranged by size:
3678 2426 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2492 3297 4783
Size: 107 122 71 631 605 820 805 1486
Cuts at: 4783 136
Size: 1525
Fragments arranged by size:
1525 1486 820 805 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 2111 2432 3589 2111
Size: 321 1157 4694
Fragments arranged by size:
4694 1157 321
Bme1580I G_kGCm'C
Cuts at: 1790 3059 3102 3129 3302 3331 3411 4233 5389
Size: 1269 43 27 173 29 80 822 1156
Cuts at: 5389 1790
Size: 2573
Fragments arranged by size:
2573 1269 1156 822 173 80 43 29
27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 3298 3903
Size: 416 53 83 399 24 1700 181 605
Cuts at: 3903 4861 442
Size: 958 1753
Fragments arranged by size:
1753 1700 958 605 416 399 181 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2849 3035 3239 3284 3347 4832 1239
Size: 1610 186 204 45 63 1485 2579
Fragments arranged by size:
2579 1610 1485 204 186 63 45
Bpu10I CC'TnA_GC
Cuts at: 2655 3005 2655
Size: 350 5822
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 2571 3011 4297 5162 5460 2054
Size: 517 440 1286 865 298 2766
Fragments arranged by size:
2766 1286 865 517 440 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2825 4835 882
Size: 617 1326 2010 2219
Fragments arranged by size:
2219 2010 1326 617
BsaAI yAC'GTr
Cuts at: 493 1204 3963 493
Size: 711 2759 2702
Fragments arranged by size:
2759 2702 711
BsaBI GATnn'nnATC
Cuts at: 6166 6166
Size: 6172
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3035 3242 3979 4361
Size: 53 83 186 936 1502 207 737 382
Cuts at: 4361 4996 275
Size: 635 1451
Fragments arranged by size:
1502 1451 936 737 635 382 207 186
83 53
BsaWI w'CCGG_w
Cuts at: 4605 5003 5277 5424 5835 4605
Size: 398 274 147 411 4942
Fragments arranged by size:
4942 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2190 2220 1178
Size: 30 165 30 787 30 5130
Fragments arranged by size:
5130 787 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2537 2651 3176 3236 1186
Size: 1351 114 525 60 4122
Fragments arranged by size:
4122 1351 525 114 60
BseYI C'CCAG_C
Cuts at: 2330 2335 2474 2498 5375 5871 2330
Size: 5 139 24 2877 496 2631
Fragments arranged by size:
2877 2631 496 139 24 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2612 3272 1163
Size: 573 876 660 4063
Fragments arranged by size:
4063 876 660 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2627 3622 4383 5411 1540
Size: 275 186 626 995 761 1028 2301
Fragments arranged by size:
2301 1028 995 761 626 275 186
BsiHKAI G_wGCw'C
Cuts at: 2612 2656 2940 3129 3210 4233 4318 5389 2612
Size: 44 284 189 81 1023 85 1071 3395
Fragments arranged by size:
3395 1071 1023 284 189 85 81 44
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2412 2612 2656 2940 3059 3102 3129
Size: 399 622 200 44 284 119 43 27
Cuts at: 3129 3210 3302 3331 3411 3491 4233 4318 5389
Size: 81 92 29 80 80 742 85 1071
Cuts at: 5389 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 622 399 284 200 119
92 85 81 80 80 44 43 29
27
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 4061 5791 4061
Size: 1730 4442
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2976 2993 3527 844
Size: 2132 17 534 3489
Fragments arranged by size:
3489 2132 534 17
BsrBI CCG'CTC
Cuts at: 1394 1823 2201 2785 3226 4059 1394
Size: 429 378 584 441 833 3507
Fragments arranged by size:
3507 833 584 441 429 378
BsrDI GCAATG_nn'
Cuts at: 65 1248 2485 4667 4841 65
Size: 1183 1237 2182 174 1396
Fragments arranged by size:
2182 1396 1237 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2019 2271 2682 3231 4816 5003 1700
Size: 110 209 252 411 549 1585 187 2869
Fragments arranged by size:
2869 1585 549 411 252 209 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 2048 1925
Size: 123 6049
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3175 794
Size: 2381 3791
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 2740 3367 1184
Size: 1556 627 3989
Fragments arranged by size:
3989 1556 627
BstYI r'GATC_y
Cuts at: 1972 3502 3869 4254 4271 5723 5809 5821 6167
Size: 1530 367 385 17 1452 86 12 346
Cuts at: 6167 1972
Size: 1977
Fragments arranged by size:
1977 1530 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2163 2214 2163
Size: 51 6121
BtgI C'CryG_G
Cuts at: 513 1065 2004 2037 2096 2310 2490 2745 513
Size: 552 939 33 59 214 180 255 3940
Fragments arranged by size:
3940 939 552 255 214 180 59 33
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5433
Fragments arranged by size:
5433 683 56
BtsI GCAGTG_nn'
Cuts at: 2139 2151 2211 2443 3196 3255 3395 3688 4482
Size: 12 60 232 753 59 140 293 794
Cuts at: 4482 4502 2139
Size: 20 3809
Fragments arranged by size:
3809 794 753 293 232 140 60 59
20 12
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2567 5179 809
Size: 507 1251 2612 1802
Fragments arranged by size:
2612 1802 1251 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2187 2273 2624
Size: 54 1425 50 275 18 357 86 351
Cuts at: 2624 3117 3402 3619 4508 6137 8
Size: 493 285 217 889 1629 43
Fragments arranged by size:
1629 1425 889 493 357 351 285 275
217 86 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2624 3619 1537
Size: 275 812 995 4090
Fragments arranged by size:
4090 995 812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2783 3153 4102 1353
Size: 1430 370 949 3423
Fragments arranged by size:
3423 1430 949 370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2654 2654
Size: 6172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2849 3035 3239 3284 3347 4235 4832
Size: 393 1217 186 204 45 63 888 597
Cuts at: 4832 5619 5887 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1217 888 787 597 393 268 204
186 63 45
EcoNI CCTnn'n_nnAGG
Cuts at: 2179 2179
Size: 6172
EcoO109I rG'GnC_Cy
Cuts at: 2159 2398 2902 2994 3328 3408 2159
Size: 239 504 92 334 80 4923
Fragments arranged by size:
4923 504 334 239 92 80
EcoRI G'AATT_C
Cuts at: 3481 3859 3481
Size: 378 5794
FspI TGC'GCA
Cuts at: 4678 4678
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2033 2381 2732 3038 5319 1678
Size: 355 348 351 306 2281 2531
Fragments arranged by size:
2531 2281 355 351 348 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 4813 4845 4887
Size: 32 135 32 1055 32 2154 32 42
Cuts at: 4887 4919 1373
Size: 32 2626
Fragments arranged by size:
2626 2154 1055 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3516 3762 669
Size: 988 281 1578 246 3079
Fragments arranged by size:
3079 1578 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2124 748
Size: 1376 4796
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KasI G'GCGC_C
Cuts at: 3034 3034
Size: 6172
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2880 5286 5470 1305
Size: 1575 2406 184 2007
Fragments arranged by size:
2406 2007 1575 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3119 10
Size: 54 1425 343 1287 3063
Fragments arranged by size:
3063 1425 1287 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2144 2507 3132 4130 4489 4648 518
Size: 648 978 363 625 998 359 159 2042
Fragments arranged by size:
2042 998 978 648 625 363 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2431 2820 3009 3217 3358 3588 4265
Size: 492 333 389 189 208 141 230 677
Cuts at: 4265 5413 5658 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 492 389 333 245 230
208 189 141
NaeI GCC'GGC
Cuts at: 2021 2021
Size: 6172
NarI GG'CG_CC
Cuts at: 3035 3035
Size: 6172
NcoI C'CATG_G
Cuts at: 513 1065 2004 2490 513
Size: 552 939 486 4195
Fragments arranged by size:
4195 939 552 486
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NgoMIV G'CCGG_C
Cuts at: 2019 2019
Size: 6172
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 2624 3619 2624
Size: 995 5177
NspI r_CATG'y
Cuts at: 1132 1229 3530 5075 1132
Size: 97 2301 1545 2229
Fragments arranged by size:
2301 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3460 1348
Size: 2112 4060
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3217 1108
Size: 2109 4063
PfoI T'CCnGG_A
Cuts at: 2826 2826
Size: 6172
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2159 2902 2159
Size: 743 5429
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6172
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PspOMI G'GGCC_C
Cuts at: 3327 3407 3327
Size: 80 6092
PstI C_TGCA'G
Cuts at: 830 2016 2146 2973 3201 3524 830
Size: 1186 130 827 228 323 3478
Fragments arranged by size:
3478 1186 827 323 228 130
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 3009 3217 3358 3009
Size: 208 141 5823
Fragments arranged by size:
5823 208 141
SacI G_AGCT'C
Cuts at: 2656 2656
Size: 6172
SacII CC_GC'GG
Cuts at: 2099 2099
Size: 6172
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SbfI CC_TGCA'GG
Cuts at: 3524 3524
Size: 6172
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SfcI C'TryA_G
Cuts at: 826 1045 2012 2142 2815 2969 3197 3520 4655
Size: 219 967 130 673 154 228 323 1135
Cuts at: 4655 5336 5527 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 967 681 673 323 228 219
191 154 130
SfoI GGC'GCC
Cuts at: 3036 3036
Size: 6172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 2262 2991 3499 1872
Size: 390 729 508 4545
Fragments arranged by size:
4545 729 508 390
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2586 3026 4276 5177 5439
Size: 197 926 126 517 440 1250 901 262
Cuts at: 5439 820
Size: 1553
Fragments arranged by size:
1553 1250 926 901 517 440 262 197
126
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 3530 1132
Size: 2398 3774
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 2736 3077 1517
Size: 1219 341 4612
Fragments arranged by size:
4612 1219 341
StyI C'CwwG_G
Cuts at: 513 1065 2004 2490 3039 3611 513
Size: 552 939 486 549 572 3074
Fragments arranged by size:
3074 939 572 552 549 486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2293 2849 2942 3040 3155 3317 4199
Size: 1255 713 556 93 98 115 162 882
Cuts at: 4199 4216 4369 4554 4999 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 882 713 556 445 185 162
153 115 98 93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4418 6130 96
Size: 274 80 33 51 1443 2441 1712 138
Fragments arranged by size:
2441 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2190 2222 6132 6164 2190
Size: 32 3910 32 2198
Fragments arranged by size:
3910 2198 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6172
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2752 3424 2752
Size: 672 5500
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6172
XmaI C'CCGG_G
Cuts at: 1870 2260 2989 3497 1870
Size: 390 729 508 4545
Fragments arranged by size:
4545 729 508 390
XmnI GAAnn'nnTTC
Cuts at: 4301 4301
Size: 6172
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI
SexAI SfiI SrfI SwaI