Restriction map for
pFN21AB9545
(Circular) (Six-base) MAPSORT of: pFN21AB9545.seq Check: 2468 from: 1 to: 6334
pFN21AB9545 6334 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3044 3044
Size: 6334
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2403 2586 2601 3518 4397 5781 6049 1632
Size: 771 183 15 917 879 1384 268 1917
Fragments arranged by size:
1917 1384 917 879 771 268 183 15
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 2113 3345 5233 6210 1203
Size: 910 1232 1888 977 1327
Fragments arranged by size:
1888 1327 1232 977 910
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 3447 5063 3447
Size: 1616 4718
AleI CACnn'nnGTG
Cuts at: 1166 3385 1166
Size: 2219 4115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3579 3611 3579
Size: 32 6302
AlwNI CAG_nnn'CTG
Cuts at: 4077 5649 4077
Size: 1572 4762
ApaLI G'TGCA_C
Cuts at: 3378 4391 5547 3378
Size: 1013 1156 4165
Fragments arranged by size:
4165 1156 1013
ApoI r'AATT_y
Cuts at: 1493 2158 2596 2993 3295 3321 3615 3643 3869
Size: 665 438 397 302 26 294 28 226
Cuts at: 3869 4021 6130 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 665 438 397 302 294 226
152 28 26
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2200 3659 1870
Size: 73 257 1459 4545
Fragments arranged by size:
4545 1459 257 73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3655 5110 618
Size: 325 217 627 1868 1455 1842
Fragments arranged by size:
1868 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2094 3117 3653 1391
Size: 703 1023 536 4072
Fragments arranged by size:
4072 1023 703 536
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2915 928
Size: 971 1016 4347
Fragments arranged by size:
4347 1016 971
BbvCI CC'TCA_GC
Cuts at: 2933 2933
Size: 6334
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 2230 1548
Size: 682 5652
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4945 136
Size: 107 122 71 631 605 3273 1525
Fragments arranged by size:
3273 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 2088 2130 3751 2088
Size: 42 1621 4671
Fragments arranged by size:
4671 1621 42
Bme1580I G_kGCm'C
Cuts at: 1790 3382 4395 5551 1790
Size: 1592 1013 1156 2573
Fragments arranged by size:
2573 1592 1156 1013
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3376 3439 4065
Size: 416 53 83 399 24 1959 63 626
Cuts at: 4065 5023 442
Size: 958 1753
Fragments arranged by size:
1959 1753 958 626 416 399 83 63
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4994 1239
Size: 3755 2579
Bpu10I CC'TnA_GC
Cuts at: 2933 3471 2933
Size: 538 5796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4459 5324 5622 4459
Size: 865 298 5171
Fragments arranged by size:
5171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2273 4997 882
Size: 617 774 2724 2219
Fragments arranged by size:
2724 2219 774 617
BsaAI yAC'GTr
Cuts at: 493 1204 2977 4125 493
Size: 711 1773 1148 2702
Fragments arranged by size:
2702 1773 1148 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 4523 5158 275
Size: 53 83 186 936 2608 382 635 1451
Fragments arranged by size:
2608 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3579 3609 1178
Size: 30 165 30 2176 30 3903
Fragments arranged by size:
3903 2176 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6334
BseYI C'CCAG_C
Cuts at: 3018 5537 6033 3018
Size: 2519 496 3319
Fragments arranged by size:
3319 2519 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5761
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 2704 3382 3438 3575 4395 4480 5551 2094
Size: 610 678 56 137 820 85 1071 2877
Fragments arranged by size:
2877 1071 820 678 610 137 85 56
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2704 3117 3382 3438 3575 3653
Size: 399 304 610 413 265 56 137 78
Cuts at: 3653 4395 4480 5551 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 610 413 399 304 265
137 85 78 56
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 2275 4223 5953 2275
Size: 1948 1730 2656
Fragments arranged by size:
2656 1948 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 3689 844
Size: 2200 645 3489
Fragments arranged by size:
3489 2200 645
BsrBI CCG'CTC
Cuts at: 1394 1823 4221 1394
Size: 429 2398 3507
Fragments arranged by size:
3507 2398 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2456 4829 5003 65
Size: 1183 1208 2373 174 1396
Fragments arranged by size:
2373 1396 1208 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3051 3399 4978 5165 1700
Size: 110 1241 348 1579 187 2869
Fragments arranged by size:
2869 1579 1241 348 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 2511 4394 5406 2511
Size: 1883 1012 3439
Fragments arranged by size:
3439 1883 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 2538 5138 2538
Size: 2600 3734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 3664 4031 4416 4433 5885 5971 5983 6329
Size: 1692 367 385 17 1452 86 12 346
Cuts at: 6329 1972
Size: 1977
Fragments arranged by size:
1977 1692 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 2304 3043 3102 3850 4644 4664 2304
Size: 739 59 748 794 20 3974
Fragments arranged by size:
3974 794 748 739 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 2926 3638 2926
Size: 712 5622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3781 4670 6299
Size: 54 1425 50 275 18 1951 889 1629
Cuts at: 6299 8
Size: 43
Fragments arranged by size:
1951 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2019 2422 3537 4264 1353
Size: 666 403 1115 727 3423
Fragments arranged by size:
3423 1115 727 666 403
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2092 2092
Size: 6334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2403 2586 2601 3518 4397 4994 5781
Size: 393 771 183 15 917 879 597 787
Cuts at: 5781 6049 1239
Size: 268 1524
Fragments arranged by size:
1524 917 879 787 771 597 393 268
183 15
EcoO109I rG'GnC_Cy
Cuts at: 3163 3163
Size: 6334
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 5481 1678
Size: 3803 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2351 2383 2812 2844 3228
Size: 32 135 32 779 32 429 32 384
Cuts at: 3228 3260 3433 3465 3542 3574 4975 5007 5049
Size: 32 173 32 77 32 1401 32 42
Cuts at: 5049 5081 1373
Size: 32 2626
Fragments arranged by size:
2626 1401 779 429 384 173 135 77
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3678 3924 669
Size: 988 281 1740 246 3079
Fragments arranged by size:
3079 1740 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5448 5632 1305
Size: 4143 184 2007
Fragments arranged by size:
4143 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4512
Fragments arranged by size:
4512 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 3346 3385 3566 4292 4651 4810
Size: 648 952 1228 39 181 726 359 159
Cuts at: 4810 518
Size: 2042
Fragments arranged by size:
2042 1228 952 726 648 359 181 159
39
MspA1I CmG'CkG
Cuts at: 1606 2937 3018 3750 4427 5575 5820 1606
Size: 1331 81 732 677 1148 245 2120
Fragments arranged by size:
2120 1331 1148 732 677 245 81
NaeI GCC'GGC
Cuts at: 3401 3401
Size: 6334
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NgoMIV G'CCGG_C
Cuts at: 3399 3399
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2117 3349 3692 5237 1132
Size: 97 888 1232 343 1545 2229
Fragments arranged by size:
2229 1545 1232 888 343 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 2113 3345 5233 2113
Size: 1232 1888 3214
Fragments arranged by size:
3214 1888 1232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PmlI CAC'GTG
Cuts at: 2977 2977
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3579 3611 4403 4435 5916 5948 3579
Size: 32 792 32 1481 32 3965
Fragments arranged by size:
3965 1481 792 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3163 3163
Size: 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PstI C_TGCA'G
Cuts at: 830 3686 830
Size: 2856 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2937 3018 2937
Size: 81 6253
SacI G_AGCT'C
Cuts at: 2094 2094
Size: 6334
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SfcI C'TryA_G
Cuts at: 826 1045 3077 3404 3682 4817 5498 5689 826
Size: 219 2032 327 278 1135 681 191 1471
Fragments arranged by size:
2032 1471 1135 681 327 278 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 2202 3661 1872
Size: 330 1459 4545
Fragments arranged by size:
4545 1459 330
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4438 5339 5601 820
Size: 197 926 2495 901 262 1553
Fragments arranged by size:
2495 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2004 3773 513
Size: 552 939 1769 3074
Fragments arranged by size:
3074 1769 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3232 4361 4378 4531 4716 5161 325
Size: 1255 1652 1129 17 153 185 445 1498
Fragments arranged by size:
1652 1498 1255 1129 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2474 2651 4580
Size: 274 80 33 51 1443 497 177 1929
Cuts at: 4580 6292 96
Size: 1712 138
Fragments arranged by size:
1929 1712 1443 497 274 177 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 6294 6326 3374
Size: 32 2888 32 3382
Fragments arranged by size:
3382 2888 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2693 3194 2693
Size: 501 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 2200 3659 1870
Size: 330 1459 4545
Fragments arranged by size:
4545 1459 330
XmnI GAAnn'nnTTC
Cuts at: 2389 2599 3531 4463 2389
Size: 210 932 932 4260
Fragments arranged by size:
4260 932 932 210
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PfoI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI