Restriction map for
pFN21AB9544
(Circular) (Six-base) MAPSORT of: pFN21AB9544.seq Check: 1385 from: 1 to: 6508
pFN21AB9544 6508 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4318 278
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2318 3851 1937
Size: 381 1533 4594
Fragments arranged by size:
4594 1533 381
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 2107 4635 5008 1563
Size: 544 2528 373 3063
Fragments arranged by size:
3063 2528 544 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3572 4571 5955 6223 1632
Size: 1185 755 999 1384 268 1917
Fragments arranged by size:
1917 1384 1185 999 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 3234 5407 6384 1203
Size: 2031 2173 977 1327
Fragments arranged by size:
2173 2031 1327 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 3425 1166
Size: 2259 4249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6508
AlwNI CAG_nnn'CTG
Cuts at: 3391 4251 5823 3391
Size: 860 1572 4076
Fragments arranged by size:
4076 1572 860
ApaLI G'TGCA_C
Cuts at: 4565 5721 4565
Size: 1156 5352
ApoI r'AATT_y
Cuts at: 1493 2161 3817 4043 4195 6304 1493
Size: 668 1656 226 152 2109 1697
Fragments arranged by size:
2109 1697 1656 668 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5062 160
Size: 872 4030 1606
Fragments arranged by size:
4030 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 2688 3833 1870
Size: 73 745 1145 4545
Fragments arranged by size:
4545 1145 745 73
AvrII C'CTAG_G
Cuts at: 2713 2713
Size: 6508
BamHI G'GATC_C
Cuts at: 3838 4205 3838
Size: 367 6141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3829 5284 618
Size: 325 217 627 2042 1455 1842
Fragments arranged by size:
2042 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3567 3827 1391
Size: 2176 260 4072
Fragments arranged by size:
4072 2176 260
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5537
BbvCI CC'TCA_GC
Cuts at: 3330 3330
Size: 6508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4699 4733 1903
Size: 34 2762 34 3678
Fragments arranged by size:
3678 2762 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 2050 1548
Size: 502 6006
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2799 5119 136
Size: 107 122 71 631 605 1127 2320 1525
Fragments arranged by size:
2320 1525 1127 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6503 6503
Size: 6508
BlpI GC'TnA_GC
Cuts at: 3925 3925
Size: 6508
Bme1580I G_kGCm'C
Cuts at: 1790 3700 4569 5725 1790
Size: 1910 869 1156 2573
Fragments arranged by size:
2573 1910 1156 869
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2403 2815 4239
Size: 416 53 83 399 24 986 412 1424
Cuts at: 4239 5197 442
Size: 958 1753
Fragments arranged by size:
1753 1424 986 958 416 412 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2422 2422
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3132 5168 1239
Size: 1893 2036 2579
Fragments arranged by size:
2579 2036 1893
Bpu10I CC'TnA_GC
Cuts at: 2151 3330 3608 2151
Size: 1179 278 5051
Fragments arranged by size:
5051 1179 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4633 5498 5796 4633
Size: 865 298 5345
Fragments arranged by size:
5345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5171 882
Size: 617 3672 2219
Fragments arranged by size:
3672 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4299 493
Size: 711 3095 2702
Fragments arranged by size:
3095 2702 711
BsaBI GATnn'nnATC
Cuts at: 2049 6502 2049
Size: 4453 2055
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2814 4315 4697 5332
Size: 53 83 186 936 1281 1501 382 635
Cuts at: 5332 275
Size: 1451
Fragments arranged by size:
1501 1451 1281 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4941 5339 5613 5760 6171 4941
Size: 398 274 147 411 5278
Fragments arranged by size:
5278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6283
Fragments arranged by size:
6283 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2230 2642 3081 3225 1186
Size: 1044 412 439 144 4469
Fragments arranged by size:
4469 1044 439 412 144
BseYI C'CCAG_C
Cuts at: 2915 3095 5711 6207 2915
Size: 180 2616 496 3216
Fragments arranged by size:
3216 2616 496 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5935
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3958 4719 5747 1540
Size: 275 186 1957 761 1028 2301
Fragments arranged by size:
2301 1957 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2445 4569 4654 5725 2445
Size: 2124 85 1071 3228
Fragments arranged by size:
3228 2124 1071 85
BsmI GAATG_Cn'
Cuts at: 4019 4112 4019
Size: 93 6415
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2445 3567 3700 3827 4569 4654 5725
Size: 399 655 1122 133 127 742 85 1071
Cuts at: 5725 1391
Size: 2174
Fragments arranged by size:
2174 1122 1071 742 655 399 133 127
85
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 4397 6127 4397
Size: 1730 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3863 844
Size: 3019 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4395 1394
Size: 429 2572 3507
Fragments arranged by size:
3507 2572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3783 5003 5177 65
Size: 1183 2535 1220 174 1396
Fragments arranged by size:
2535 1396 1220 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5152 5339 1700
Size: 110 3342 187 2869
Fragments arranged by size:
3342 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 4568 5580 4568
Size: 1012 5496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6508
BstBI TT'CG_AA
Cuts at: 5312 5312
Size: 6508
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6508
BstXI CCAn_nnnn'nTGG
Cuts at: 3155 3155
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 3838 4205 4590 4607 6059 6145 6157 6503
Size: 1866 367 385 17 1452 86 12 346
Cuts at: 6503 1972
Size: 1977
Fragments arranged by size:
1977 1866 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5017
Fragments arranged by size:
5017 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 2476 4024 4818 4838 2476
Size: 1548 794 20 4146
Fragments arranged by size:
4146 1548 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6473
DraI TTT'AAA
Cuts at: 2574 3812 2574
Size: 1238 5270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2083 3955 4844
Size: 54 1425 50 275 18 253 1872 889
Cuts at: 4844 6473 8
Size: 1629 43
Fragments arranged by size:
1872 1629 1425 889 275 253 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3955 1537
Size: 275 2143 4090
Fragments arranged by size:
4090 2143 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 2222 2419 2969 3180 3481 4438 1353
Size: 774 95 197 550 211 301 957 3423
Fragments arranged by size:
3423 957 774 550 301 211 197 95
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5095 5469 5615 387
Size: 1064 138 3506 374 146 1280
Fragments arranged by size:
3506 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2817 3132 3572 4571 5168 5955 6223
Size: 393 1185 315 440 999 597 787 268
Cuts at: 6223 1239
Size: 1524
Fragments arranged by size:
1524 1185 999 787 597 440 393 315
268
EcoRI G'AATT_C
Cuts at: 2161 3817 4195 2161
Size: 1656 378 4474
Fragments arranged by size:
4474 1656 378
FspI TGC'GCA
Cuts at: 5014 5014
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 5655 1678
Size: 3977 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2287 2483 2515 2615
Size: 32 135 32 683 32 196 32 100
Cuts at: 2615 2647 2879 2911 5149 5181 5223 5255 1373
Size: 32 232 32 2238 32 42 32 2626
Fragments arranged by size:
2626 2238 683 232 196 135 100 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3852 4098 669
Size: 988 281 1914 246 3079
Fragments arranged by size:
3079 1914 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6508
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 4107 4107
Size: 6508
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5622 5806 1305
Size: 4317 184 2007
Fragments arranged by size:
4317 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4686
Fragments arranged by size:
4686 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3153 3425 3521 4466 4825 4984 518
Size: 648 1987 272 96 945 359 159 2042
Fragments arranged by size:
2042 1987 945 648 359 272 159 96
MspA1I CmG'CkG
Cuts at: 1606 2081 3592 3924 4601 5749 5994 1606
Size: 475 1511 332 677 1148 245 2120
Fragments arranged by size:
2120 1511 1148 677 475 332 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5017
Fragments arranged by size:
5017 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6508
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 3238 3717 3866 5411 1132
Size: 97 2009 479 149 1545 2229
Fragments arranged by size:
2229 2009 1545 479 149 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6508
PciI A'CATG_T
Cuts at: 3234 5407 3234
Size: 2173 4335
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3110 3140 1108
Size: 2002 30 4476
Fragments arranged by size:
4476 2002 30
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4577 4609 6090 6122 4577
Size: 32 1481 32 4963
Fragments arranged by size:
4963 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6508
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PstI C_TGCA'G
Cuts at: 830 3396 3633 3860 830
Size: 2566 237 227 3478
Fragments arranged by size:
3478 2566 237 227
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6508
PvuII CAG'CTG
Cuts at: 2081 3592 2081
Size: 1511 4997
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SapI GCTCTTCn'nnn_
Cuts at: 2419 2969 2419
Size: 550 5958
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 3489 4756 6468 3489
Size: 1267 1712 3529
Fragments arranged by size:
3529 1712 1267
SexAI A'CCwGG_T
Cuts at: 3246 3246
Size: 6508
SfcI C'TryA_G
Cuts at: 826 1045 3392 3480 3629 3856 4991 5672 5863
Size: 219 2347 88 149 227 1135 681 191
Cuts at: 5863 826
Size: 1471
Fragments arranged by size:
2347 1471 1135 681 227 219 191 149
88
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3835 1872
Size: 1963 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4612 5513 5775 820
Size: 197 926 2669 901 262 1553
Fragments arranged by size:
2669 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4299 493
Size: 3806 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 4432 5
Size: 47 4380 2081
Fragments arranged by size:
4380 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6508
StyI C'CwwG_G
Cuts at: 513 1065 2004 2713 3700 3708 3947 513
Size: 552 939 709 987 8 239 3074
Fragments arranged by size:
3074 987 939 709 552 239 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3529 4535 4552 4705 4890 5335 325
Size: 1255 1949 1006 17 153 185 445 1498
Fragments arranged by size:
1949 1498 1255 1006 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3487 4754 6466
Size: 274 80 33 51 1443 1510 1267 1712
Cuts at: 6466 96
Size: 138
Fragments arranged by size:
1712 1510 1443 1267 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2750 2782 3011 3043 6468 6500 2750
Size: 32 229 32 3425 32 2758
Fragments arranged by size:
3425 2758 229 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3624 1316
Size: 2308 4200
XbaI T'CTAG_A
Cuts at: 3844 3844
Size: 6508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6508
XmaI C'CCGG_G
Cuts at: 1870 3833 1870
Size: 1963 4545
XmnI GAAnn'nnTTC
Cuts at: 2871 4637 2871
Size: 1766 4742
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4316 276
Size: 53 83 186 936 2782 2468
Fragments arranged by size:
2782 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI