Restriction map for
pFN21AB9540
(Circular) (Six-base) MAPSORT of: pFN21AB9540.seq Check: 9727 from: 1 to: 5758
pFN21AB9540 5758 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 2886 3079 2886
Size: 193 5565
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2106 2592 2631 3821 5205 5473 1632
Size: 474 486 39 1190 1384 268 1917
Fragments arranged by size:
1917 1384 1190 486 474 268 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 2053 4487 2053
Size: 2434 3324
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5758
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 3004 3501 5073 3004
Size: 497 1572 3689
Fragments arranged by size:
3689 1572 497
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 3083 1870
Size: 73 1140 4545
Fragments arranged by size:
4545 1140 73
AvrII C'CTAG_G
Cuts at: 2095 2095
Size: 5758
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2886 3079 4534 618
Size: 325 217 627 1099 193 1455 1842
Fragments arranged by size:
1842 1455 1099 627 325 217 193
BanII G_rGCy'C
Cuts at: 1391 2177 2678 2726 3077 1391
Size: 786 501 48 351 4072
Fragments arranged by size:
4072 786 501 351 48
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2643 928
Size: 971 744 4043
Fragments arranged by size:
4043 971 744
BbvCI CC'TCA_GC
Cuts at: 2700 2700
Size: 5758
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2276 2310 2447 2481 3949 3983 1903
Size: 34 339 34 137 34 1468 34 3678
Fragments arranged by size:
3678 1468 339 137 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 3819 4975 1790
Size: 2029 1156 2573
Fragments arranged by size:
2573 2029 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3489 4447 442
Size: 416 53 83 399 24 2072 958 1753
Fragments arranged by size:
2072 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2137 2138 2508 2733 2817 4418 1239
Size: 898 1 370 225 84 1601 2579
Fragments arranged by size:
2579 1601 898 370 225 84 1
Bpu10I CC'TnA_GC
Cuts at: 2024 2166 2700 2024
Size: 142 534 5082
Fragments arranged by size:
5082 534 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3883 4748 5046 3883
Size: 865 298 4595
Fragments arranged by size:
4595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2105 2700 4421 882
Size: 617 606 595 1721 2219
Fragments arranged by size:
2219 1721 617 606 595
BsaAI yAC'GTr
Cuts at: 493 1204 3549 493
Size: 711 2345 2702
Fragments arranged by size:
2702 2345 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2984 3014 1178
Size: 30 165 30 1581 30 3922
Fragments arranged by size:
3922 1581 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2167 2688 1186
Size: 981 521 4256
Fragments arranged by size:
4256 981 521
BseYI C'CCAG_C
Cuts at: 2548 4961 5457 2548
Size: 2413 496 2849
Fragments arranged by size:
2849 2413 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3819 3904 4975 3819
Size: 85 1071 4602
Fragments arranged by size:
4602 1071 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2177 2678 2726 3077 3819 3904 4975
Size: 399 387 501 48 351 742 85 1071
Cuts at: 4975 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 501 399 387 351 85
48
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3113 844
Size: 2269 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4253 4427 65
Size: 1183 3005 174 1396
Fragments arranged by size:
3005 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2994 3088 3455 3840 3857 5309 5395 5407
Size: 1022 94 367 385 17 1452 86 12
Cuts at: 5407 5753 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1022 385 367 346 94 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5206
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 2560 2595 3274 4068 4088 2560
Size: 35 679 794 20 4230
Fragments arranged by size:
4230 794 679 35 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2124 2428 2985
Size: 54 1425 50 275 18 294 304 557
Cuts at: 2985 3205 4094 5723 8
Size: 220 889 1629 43
Fragments arranged by size:
1629 1425 889 557 304 294 275 220
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 2894 3015 3688 1353
Size: 1143 398 121 673 3423
Fragments arranged by size:
3423 1143 673 398 121
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2921 2990 4345 4719 4865 387
Size: 1064 138 1332 69 1355 374 146 1280
Fragments arranged by size:
1355 1332 1280 1064 374 146 138 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2106 2137 2138 2508 2592 2631 2733
Size: 393 474 31 1 370 84 39 102
Cuts at: 2733 2817 3821 4418 5205 5473 1239
Size: 84 1004 597 787 268 1524
Fragments arranged by size:
1524 1004 787 597 474 393 370 268
102 84 84 39 31 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2384 2716 2987 2384
Size: 332 271 5155
Fragments arranged by size:
5155 332 271
EcoO109I rG'GnC_Cy
Cuts at: 2509 3052 2509
Size: 543 5215
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
EcoRV GAT'ATC
Cuts at: 2566 2566
Size: 5758
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2039 2071 2225 2257 4399
Size: 32 135 32 467 32 154 32 2142
Cuts at: 4399 4431 4473 4505 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2142 467 154 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 2890 3083 2890
Size: 193 5565
MfeI C'AATT_G
Cuts at: 2424 3357 2424
Size: 933 4825
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2241 2331 4872 5056 1305
Size: 936 90 2541 184 2007
Fragments arranged by size:
2541 2007 936 184 90
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2126 2430 2987 10
Size: 54 1425 343 294 304 557 2781
Fragments arranged by size:
2781 1425 557 343 304 294 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2669 3716 4075 4234 518
Size: 648 1503 1047 359 159 2042
Fragments arranged by size:
2042 1503 1047 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3174 3851 4999 5244 1606
Size: 1568 677 1148 245 2120
Fragments arranged by size:
2120 1568 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5206
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 3116 4661 1132
Size: 97 1887 1545 2229
Fragments arranged by size:
2229 1887 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2504 1348
Size: 1156 4602
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PfoI T'CCnGG_A
Cuts at: 2243 2385 2794 2243
Size: 142 409 5207
Fragments arranged by size:
5207 409 142
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2509 3052 2509
Size: 543 5215
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 3110 830
Size: 2280 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SanDI GG'GwC_CC
Cuts at: 3052 3052
Size: 5758
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 2460 2460
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 3106 4241 4922 5113 826
Size: 219 2061 1135 681 191 1471
Fragments arranged by size:
2061 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3862 4763 5025 820
Size: 197 926 1919 901 262 1553
Fragments arranged by size:
1919 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2095 2988 3197 513
Size: 552 1030 893 209 3074
Fragments arranged by size:
3074 1030 893 552 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 3785 3802 3955 4140 4585 325
Size: 1255 444 1761 17 153 185 445 1498
Fragments arranged by size:
1761 1498 1255 445 444 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4004 5716 96
Size: 274 80 33 51 1443 2027 1712 138
Fragments arranged by size:
2027 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 2282 3887 2282
Size: 1605 4153
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI XcmI