Restriction map for
pFN21AB9536
(Circular) (Six-base) MAPSORT of: pFN21AB9536.seq Check: 1853 from: 1 to: 5911
pFN21AB9536 5911 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3721 278
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3254 1937
Size: 1317 4594
Acc65I G'GTAC_C
Cuts at: 3232 3232
Size: 5911
AclI AA'CG_TT
Cuts at: 1563 4038 4411 1563
Size: 2475 373 3063
Fragments arranged by size:
3063 2475 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1998 2479 3974 5358 5626 1632
Size: 366 481 1495 1384 268 1917
Fragments arranged by size:
1917 1495 1384 481 366 268
AfeI AGC'GCT
Cuts at: 2343 2343
Size: 5911
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5714
AflIII A'CryG_T
Cuts at: 1203 2387 4810 5787 1203
Size: 1184 2423 977 1327
Fragments arranged by size:
2423 1327 1184 977
AgeI A'CCGG_T
Cuts at: 4742 4742
Size: 5911
AhdI GACnn_n'nnGTC
Cuts at: 4640 4640
Size: 5911
AleI CACnn'nnGTG
Cuts at: 1166 2538 2790 2968 3126 1166
Size: 1372 252 178 158 3951
Fragments arranged by size:
3951 1372 252 178 158
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5911
AlwNI CAG_nnn'CTG
Cuts at: 3654 5226 3654
Size: 1572 4339
ApaLI G'TGCA_C
Cuts at: 2771 3968 5124 2771
Size: 1197 1156 3558
Fragments arranged by size:
3558 1197 1156
ApoI r'AATT_y
Cuts at: 1493 2378 2407 2943 3220 3446 3598 5707 1493
Size: 885 29 536 277 226 152 2109 1697
Fragments arranged by size:
2109 1697 885 536 277 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 4465 160
Size: 872 3433 1606
Fragments arranged by size:
3433 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
AvaI C'yCGr_G
Cuts at: 1870 1943 2561 3236 1870
Size: 73 618 675 4545
Fragments arranged by size:
4545 675 618 73
AvrII C'CTAG_G
Cuts at: 2998 2998
Size: 5911
BamHI G'GATC_C
Cuts at: 2277 3241 3608 2277
Size: 964 367 4580
Fragments arranged by size:
4580 964 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2729 2856 3232 4687 618
Size: 325 217 627 942 127 376 1455 1842
Fragments arranged by size:
1842 1455 942 627 376 325 217 127
BanII G_rGCy'C
Cuts at: 1391 3230 1391
Size: 1839 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4940
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4102 4136 1903
Size: 34 2165 34 3678
Fragments arranged by size:
3678 2165 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3803 5013 3803
Size: 1210 4701
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5911
BfrBI ATG'CAT
Cuts at: 2101 2101
Size: 5911
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2454 4522 136
Size: 107 122 71 631 605 782 2068 1525
Fragments arranged by size:
2068 1525 782 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2257 2593 2923 5906 2257
Size: 336 330 2983 2262
Fragments arranged by size:
2983 2262 336 330
BlpI GC'TnA_GC
Cuts at: 3173 3328 3173
Size: 155 5756
Bme1580I G_kGCm'C
Cuts at: 1790 2775 3972 5128 1790
Size: 985 1197 1156 2573
Fragments arranged by size:
2573 1197 1156 985
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5911
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2211 2631 2715
Size: 416 53 83 399 24 794 420 84
Cuts at: 2715 3642 4600 442
Size: 927 958 1753
Fragments arranged by size:
1753 958 927 794 420 416 399 84
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5911
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4571 1239
Size: 3332 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3188 4036 4901 5199 3188
Size: 848 865 298 3900
Fragments arranged by size:
3900 865 848 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4574 882
Size: 617 3075 2219
Fragments arranged by size:
3075 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3702 493
Size: 711 2498 2702
Fragments arranged by size:
2702 2498 711
BsaBI GATnn'nnATC
Cuts at: 5905 5905
Size: 5911
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3718 4100 4735 275
Size: 53 83 186 936 2185 382 635 1451
Fragments arranged by size:
2185 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4344 4742 5016 5163 5574 4344
Size: 398 274 147 411 4681
Fragments arranged by size:
4681 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5686
Fragments arranged by size:
5686 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3059 1186
Size: 1873 4038
BseYI C'CCAG_C
Cuts at: 5114 5610 5114
Size: 496 5415
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5338
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3361 4122 5150 1540
Size: 275 186 1360 761 1028 2301
Fragments arranged by size:
2301 1360 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2775 3972 4057 5128 2775
Size: 1197 85 1071 3558
Fragments arranged by size:
3558 1197 1071 85
BsmI GAATG_Cn'
Cuts at: 2150 3422 3515 2150
Size: 1272 93 4546
Fragments arranged by size:
4546 1272 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2775 3230 3972 4057 5128 1391
Size: 399 985 455 742 85 1071 2174
Fragments arranged by size:
2174 1071 985 742 455 399 85
BspEI T'CCGG_A
Cuts at: 5574 5574
Size: 5911
BspHI T'CATG_A
Cuts at: 3800 5530 3800
Size: 1730 4181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2360 3266 844
Size: 1516 906 3489
Fragments arranged by size:
3489 1516 906
BsrBI CCG'CTC
Cuts at: 1394 1823 3798 1394
Size: 429 1975 3507
Fragments arranged by size:
3507 1975 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4406 4580 65
Size: 1183 3158 174 1396
Fragments arranged by size:
3158 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4555 4742 1700
Size: 110 2745 187 2869
Fragments arranged by size:
2869 2745 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 2686 2932 96
Size: 1911 679 246 3075
Fragments arranged by size:
3075 1911 679 246
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5911
BssSI C'ACGA_G
Cuts at: 3971 4983 3971
Size: 1012 4899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5911
BstBI TT'CG_AA
Cuts at: 4715 4715
Size: 5911
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5911
BstXI CCAn_nnnn'nTGG
Cuts at: 2970 2970
Size: 5911
BstYI r'GATC_y
Cuts at: 1972 2257 2277 2593 2923 3241 3608 3993 4010
Size: 285 20 316 330 318 367 385 17
Cuts at: 4010 5462 5548 5560 5906 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 330 318 316
285 86 20 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5359
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5172
Fragments arranged by size:
5172 683 56
BtsI GCAGTG_nn'
Cuts at: 3427 4221 4241 3427
Size: 794 20 5097
Fragments arranged by size:
5097 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5911
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5876
DraI TTT'AAA
Cuts at: 2884 3215 2884
Size: 331 5580
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4918 809
Size: 507 3602 1802
Fragments arranged by size:
3602 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2749 3358 4247
Size: 54 1425 50 275 18 919 609 889
Cuts at: 4247 5876 8
Size: 1629 43
Fragments arranged by size:
1629 1425 919 889 609 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3358 1537
Size: 275 1546 4090
Fragments arranged by size:
4090 1546 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2013 2315 2949 3841 1353
Size: 660 302 634 892 3423
Fragments arranged by size:
3423 892 660 634 302
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4498 4872 5018 387
Size: 1064 138 2909 374 146 1280
Fragments arranged by size:
2909 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2479 3974 4571 5358 5626 1239
Size: 393 366 481 1495 597 787 268 1524
Fragments arranged by size:
1524 1495 787 597 481 393 366 268
EcoO109I rG'GnC_Cy
Cuts at: 2631 2631
Size: 5911
EcoRI G'AATT_C
Cuts at: 2943 3220 3598 2943
Size: 277 378 5256
Fragments arranged by size:
5256 378 277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2173 2173
Size: 5911
FspI TGC'GCA
Cuts at: 4417 4417
Size: 5911
HaeII r_GCGC'y
Cuts at: 1678 2345 5058 1678
Size: 667 2713 2531
Fragments arranged by size:
2713 2531 667
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2410 2442 4552
Size: 32 135 32 535 32 271 32 2110
Cuts at: 4552 4584 4626 4658 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2110 535 271 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3255 3501 669
Size: 988 281 1317 246 3079
Fragments arranged by size:
3079 1317 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2574 2905 3079 748
Size: 1826 331 174 3580
Fragments arranged by size:
3580 1826 331 174
HpaI GTT'AAC
Cuts at: 3501 3501
Size: 5911
KpnI G_GTAC'C
Cuts at: 3236 3236
Size: 5911
MfeI C'AATT_G
Cuts at: 3510 3510
Size: 5911
MluI A'CGCG_T
Cuts at: 5787 5787
Size: 5911
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5025 5209 1305
Size: 3720 184 2007
Fragments arranged by size:
3720 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2751 10
Size: 54 1425 343 919 3170
Fragments arranged by size:
3170 1425 919 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 2470 2538 2554 2790 2968 3126
Size: 648 934 370 68 16 236 178 158
Cuts at: 3126 3869 4228 4387 518
Size: 743 359 159 2042
Fragments arranged by size:
2042 934 743 648 370 359 236 178
159 158 68 16
MspA1I CmG'CkG
Cuts at: 1606 3327 4004 5152 5397 1606
Size: 1721 677 1148 245 2120
Fragments arranged by size:
2120 1721 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5359
NdeI CA'TA_TG
Cuts at: 387 2097 387
Size: 1710 4201
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5911
NotI GC'GGCC_GC
Cuts at: 3358 3358
Size: 5911
NsiI A_TGCA'T
Cuts at: 2103 2103
Size: 5911
NspI r_CATG'y
Cuts at: 1132 1229 2369 2391 3269 4814 1132
Size: 97 1140 22 878 1545 2229
Fragments arranged by size:
2229 1545 1140 878 97 22
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5911
PciI A'CATG_T
Cuts at: 2387 4810 2387
Size: 2423 3488
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5911
PmeI GTTT'AAAC
Cuts at: 3215 3215
Size: 5911
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3980 4012 5493 5525 3980
Size: 32 1481 32 4366
Fragments arranged by size:
4366 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5911
PsiI TTA'TAA
Cuts at: 2217 2301 3481 2217
Size: 84 1180 4647
Fragments arranged by size:
4647 1180 84
PstI C_TGCA'G
Cuts at: 830 3263 830
Size: 2433 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5911
SalI G'TCGA_C
Cuts at: 1936 3253 1936
Size: 1317 4594
SapI GCTCTTCn'nnn_
Cuts at: 2315 2315
Size: 5911
SbfI CC_TGCA'GG
Cuts at: 3263 3263
Size: 5911
ScaI AGT'ACT
Cuts at: 4159 5871 4159
Size: 1712 4199
SfcI C'TryA_G
Cuts at: 826 1045 2914 3259 4394 5075 5266 826
Size: 219 1869 345 1135 681 191 1471
Fragments arranged by size:
1869 1471 1135 681 345 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5911
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5911
SmaI CCC'GGG
Cuts at: 1872 3238 1872
Size: 1366 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2561 3167 4015 4916 5178 820
Size: 197 926 618 606 848 901 262 1553
Fragments arranged by size:
1553 926 901 848 618 606 262 197
SnaBI TAC'GTA
Cuts at: 493 3702 493
Size: 3209 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5911
SphI G_CATG'C
Cuts at: 1132 3269 1132
Size: 2137 3774
SspI AAT'ATT
Cuts at: 5 52 3835 5
Size: 47 3783 2081
Fragments arranged by size:
3783 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5911
StyI C'CwwG_G
Cuts at: 513 1065 2998 3350 513
Size: 552 1933 352 3074
Fragments arranged by size:
3074 1933 552 352
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3938 3955 4108 4293 4738 325
Size: 1255 2358 17 153 185 445 1498
Fragments arranged by size:
2358 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 2504 2686
Size: 274 80 33 51 1443 30 497 182
Cuts at: 2686 2932 3118 4157 5869 96
Size: 246 186 1039 1712 138
Fragments arranged by size:
1712 1443 1039 497 274 246 186 182
138 80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5871 5903 5871
Size: 32 5879
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5911
XbaI T'CTAG_A
Cuts at: 3247 3247
Size: 5911
XhoI C'TCGA_G
Cuts at: 1943 2561 1943
Size: 618 5293
XmaI C'CCGG_G
Cuts at: 1870 3236 1870
Size: 1366 4545
XmnI GAAnn'nnTTC
Cuts at: 4040 4040
Size: 5911
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3719 276
Size: 53 83 186 936 2185 2468
Fragments arranged by size:
2468 2185 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI