Restriction map for
pFN21AB9522
(Circular) (Six-base) MAPSORT of: pFN21AB9522.seq Check: 8962 from: 1 to: 6766
pFN21AB9522 6766 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3458 3458
Size: 6766
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4576 278
Size: 53 83 186 936 3040 2468
Fragments arranged by size:
3040 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4109 1937
Size: 2172 4594
Acc65I G'GTAC_C
Cuts at: 4087 4087
Size: 6766
AclI AA'CG_TT
Cuts at: 1563 4893 5266 1563
Size: 3330 373 3063
Fragments arranged by size:
3330 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2594 2866 3703 4022 4829 6213 6481 1632
Size: 962 272 837 319 807 1384 268 1917
Fragments arranged by size:
1917 1384 962 837 807 319 272 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6569
AflIII A'CryG_T
Cuts at: 1203 2529 3808 5665 6642 1203
Size: 1326 1279 1857 977 1327
Fragments arranged by size:
1857 1327 1326 1279 977
AgeI A'CCGG_T
Cuts at: 5597 5597
Size: 6766
AhdI GACnn_n'nnGTC
Cuts at: 2680 5495 2680
Size: 2815 3951
AleI CACnn'nnGTG
Cuts at: 1166 3761 1166
Size: 2595 4171
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6766
AlwNI CAG_nnn'CTG
Cuts at: 2020 2572 2638 2749 3398 3775 4509 6081 2020
Size: 552 66 111 649 377 734 1572 2705
Fragments arranged by size:
2705 1572 734 649 552 377 111 66
ApaI G_GGCC'C
Cuts at: 2402 2402
Size: 6766
ApaLI G'TGCA_C
Cuts at: 3197 3320 4823 5979 3197
Size: 123 1503 1156 3984
Fragments arranged by size:
3984 1503 1156 123
ApoI r'AATT_y
Cuts at: 1493 2911 4075 4301 4453 6562 1493
Size: 1418 1164 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 1164 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5320 160
Size: 872 4288 1606
Fragments arranged by size:
4288 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
AvaI C'yCGr_G
Cuts at: 1870 1943 3076 3300 3470 3981 4039 4091 1870
Size: 73 1133 224 170 511 58 52 4545
Fragments arranged by size:
4545 1133 511 224 170 73 58 52
BamHI G'GATC_C
Cuts at: 3675 4096 4463 3675
Size: 421 367 5978
Fragments arranged by size:
5978 421 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2069 3512 3884 4087 5542
Size: 325 217 627 282 1443 372 203 1455
Cuts at: 5542 618
Size: 1842
Fragments arranged by size:
1842 1455 1443 627 372 325 282 217
203
BanII G_rGCy'C
Cuts at: 1391 2402 2441 2541 2988 4085 1391
Size: 1011 39 100 447 1097 4072
Fragments arranged by size:
4072 1097 1011 447 100 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 3063 3337 928
Size: 971 201 963 274 4357
Fragments arranged by size:
4357 971 963 274 201
BbvCI CC'TCA_GC
Cuts at: 3830 3830
Size: 6766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2158 2192 3349 3383 4957 4991 1903
Size: 34 221 34 1157 34 1574 34 3678
Fragments arranged by size:
3678 1574 1157 221 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4658 5868 4658
Size: 1210 5556
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6766
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5377 136
Size: 107 122 71 631 605 3705 1525
Fragments arranged by size:
3705 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6761 6761
Size: 6766
BlpI GC'TnA_GC
Cuts at: 2561 3371 3542 4183 2561
Size: 810 171 641 5144
Fragments arranged by size:
5144 810 641 171
Bme1580I G_kGCm'C
Cuts at: 1790 2074 2402 3201 3324 4827 5983 1790
Size: 284 328 799 123 1503 1156 2573
Fragments arranged by size:
2573 1503 1156 799 328 284 123
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6766
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2008 2454 4497
Size: 416 53 83 399 24 591 446 2043
Cuts at: 4497 5455 442
Size: 958 1753
Fragments arranged by size:
2043 1753 958 591 446 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3755 1056
Size: 2699 4067
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2409 3315 2409
Size: 906 5860
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2537 2660 2708 2726 3271 3627 5426 1239
Size: 1298 123 48 18 545 356 1799 2579
Fragments arranged by size:
2579 1799 1298 545 356 123 48 18
Bpu10I CC'TnA_GC
Cuts at: 2394 3212 3830 2394
Size: 818 618 5330
Fragments arranged by size:
5330 818 618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2578 3521 4891 5756 6054 2578
Size: 943 1370 865 298 3290
Fragments arranged by size:
3290 1370 943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3098 3178 5429 882
Size: 617 1599 80 2251 2219
Fragments arranged by size:
2251 2219 1599 617 80
BsaAI yAC'GTr
Cuts at: 493 1204 3229 4557 493
Size: 711 2025 1328 2702
Fragments arranged by size:
2702 2025 1328 711
BsaBI GATnn'nnATC
Cuts at: 6760 6760
Size: 6766
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3192 3651 4573 4955
Size: 53 83 186 936 1659 459 922 382
Cuts at: 4955 5590 275
Size: 635 1451
Fragments arranged by size:
1659 1451 936 922 635 459 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2697 5199 5597 5871 6018 6429 2697
Size: 2502 398 274 147 411 3034
Fragments arranged by size:
3034 2502 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2744 2774 3269 3299 1178
Size: 30 165 30 1341 30 495 30 4645
Fragments arranged by size:
4645 1341 495 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2351 2396 2705 2786 2789 2858 3166 3209
Size: 1165 45 309 81 3 69 308 43
Cuts at: 3209 1186
Size: 4743
Fragments arranged by size:
4743 1165 309 308 81 69 45 43
3
BseYI C'CCAG_C
Cuts at: 2297 3095 3555 3823 5969 6465 2297
Size: 798 460 268 2146 496 2598
Fragments arranged by size:
2598 2146 798 496 460 268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2735 3416 3788 1163
Size: 573 999 681 372 4141
Fragments arranged by size:
4141 999 681 573 372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2508 4216 4977 6005 1540
Size: 275 186 507 1708 761 1028 2301
Fragments arranged by size:
2301 1708 1028 761 507 275 186
BsiHKAI G_wGCw'C
Cuts at: 2541 2997 3201 3324 4827 4912 5983 2541
Size: 456 204 123 1503 85 1071 3324
Fragments arranged by size:
3324 1503 1071 456 204 123 85
BsmI GAATG_Cn'
Cuts at: 4277 4370 4277
Size: 93 6673
BsmBI CGTCTCn'nnnn_
Cuts at: 3247 3247
Size: 6766
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2074 2402 2441 2541 2988 2997 3201
Size: 399 284 328 39 100 447 9 204
Cuts at: 3201 3324 4085 4827 4912 5983 1391
Size: 123 761 742 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 447 399 328 284
204 123 100 85 39 9
BspEI T'CCGG_A
Cuts at: 6429 6429
Size: 6766
BspHI T'CATG_A
Cuts at: 4655 6385 4655
Size: 1730 5036
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2172 2757 3339 3458 4121 844
Size: 1328 585 582 119 663 3489
Fragments arranged by size:
3489 1328 663 585 582 119
BsrBI CCG'CTC
Cuts at: 1394 1823 2510 4653 1394
Size: 429 687 2143 3507
Fragments arranged by size:
3507 2143 687 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3044 3267 5261 5435 65
Size: 1183 1796 223 1994 174 1396
Fragments arranged by size:
1994 1796 1396 1183 223 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2735 3379 5410 5597 1700
Size: 110 925 644 2031 187 2869
Fragments arranged by size:
2869 2031 925 644 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6766
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6766
BssSI C'ACGA_G
Cuts at: 3358 3494 4826 5838 3358
Size: 136 1332 1012 4286
Fragments arranged by size:
4286 1332 1012 136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3775 794
Size: 2981 3785
BstBI TT'CG_AA
Cuts at: 5570 5570
Size: 6766
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6766
BstXI CCAn_nnnn'nTGG
Cuts at: 3574 3625 3763 3574
Size: 51 138 6577
Fragments arranged by size:
6577 138 51
BstYI r'GATC_y
Cuts at: 1972 3675 3771 4096 4463 4848 4865 6317 6403
Size: 1703 96 325 367 385 17 1452 86
Cuts at: 6403 6415 6761 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1703 1452 385 367 346 325 96
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2074 2709 2988 3549 3759 513
Size: 552 1009 635 279 561 210 3520
Fragments arranged by size:
3520 1009 635 561 552 279 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6027
Fragments arranged by size:
6027 683 56
BtsI GCAGTG_nn'
Cuts at: 3097 3168 3818 4282 5076 5096 3097
Size: 71 650 464 794 20 4767
Fragments arranged by size:
4767 794 650 464 71 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6766
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6731
DraI TTT'AAA
Cuts at: 4070 4070
Size: 6766
DraIII CAC_nnn'GTG
Cuts at: 2715 2715
Size: 6766
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 3689 5773 809
Size: 507 698 1675 2084 1802
Fragments arranged by size:
2084 1802 1675 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3339 3744
Size: 54 1425 50 275 18 675 834 405
Cuts at: 3744 4213 5102 6731 8
Size: 469 889 1629 43
Fragments arranged by size:
1629 1425 889 834 675 469 405 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2505 4213 1537
Size: 275 693 1708 4090
Fragments arranged by size:
4090 1708 693 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2184 2828 2855 3722 3998 4696 1353
Size: 707 124 644 27 867 276 698 3423
Fragments arranged by size:
3423 867 707 698 644 276 124 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5353 5727 5873 387
Size: 1064 138 3764 374 146 1280
Fragments arranged by size:
3764 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2539 2539
Size: 6766
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2594 2660 2708 2726 2866 3271
Size: 393 905 57 66 48 18 140 405
Cuts at: 3271 3627 3703 4022 4829 5426 6213 6481 1239
Size: 356 76 319 807 597 787 268 1524
Fragments arranged by size:
1524 905 807 787 597 405 393 356
319 268 140 76 66 57 48 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2393 2596 2900 2393
Size: 203 304 6259
Fragments arranged by size:
6259 304 203
EcoO109I rG'GnC_Cy
Cuts at: 2194 2260 2398 2399 2548 2601 2746 2194
Size: 66 138 1 149 53 145 6214
Fragments arranged by size:
6214 149 145 138 66 53 1
EcoRI G'AATT_C
Cuts at: 2911 4075 4453 2911
Size: 1164 378 5224
Fragments arranged by size:
5224 1164 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2347 2347
Size: 6766
FspI TGC'GCA
Cuts at: 2168 5272 2168
Size: 3104 3662
HaeII r_GCGC'y
Cuts at: 1678 3549 4023 5913 1678
Size: 1871 474 1890 2531
Fragments arranged by size:
2531 1890 1871 474
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2293 2325 2336 2368 2666
Size: 32 135 32 721 32 11 32 298
Cuts at: 2666 2698 5407 5439 5481 5513 1373
Size: 32 2709 32 42 32 2626
Fragments arranged by size:
2709 2626 721 298 135 42 32 32
32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4110 4356 669
Size: 988 281 2172 246 3079
Fragments arranged by size:
3079 2172 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6766
HpaI GTT'AAC
Cuts at: 4356 4356
Size: 6766
KpnI G_GTAC'C
Cuts at: 4091 4091
Size: 6766
MfeI C'AATT_G
Cuts at: 4365 4365
Size: 6766
MluI A'CGCG_T
Cuts at: 6642 6642
Size: 6766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5880 6064 1305
Size: 4575 184 2007
Fragments arranged by size:
4575 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3746 10
Size: 54 1425 343 1914 3030
Fragments arranged by size:
3030 1914 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3317 3746 3761 3764 4724 5083 5242
Size: 648 2151 429 15 3 960 359 159
Cuts at: 5242 518
Size: 2042
Fragments arranged by size:
2151 2042 960 648 429 359 159 15
3
MspA1I CmG'CkG
Cuts at: 1606 2116 2407 2557 4182 4859 6007 6252 1606
Size: 510 291 150 1625 677 1148 245 2120
Fragments arranged by size:
2120 1625 1148 677 510 291 245 150
NaeI GCC'GGC
Cuts at: 2737 2737
Size: 6766
NcoI C'CATG_G
Cuts at: 513 1065 3549 3759 513
Size: 552 2484 210 3520
Fragments arranged by size:
3520 2484 552 210
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6766
NgoMIV G'CCGG_C
Cuts at: 2735 2735
Size: 6766
NheI G'CTAG_C
Cuts at: 1052 3751 1052
Size: 2699 4067
NotI GC'GGCC_GC
Cuts at: 4213 4213
Size: 6766
NspI r_CATG'y
Cuts at: 1132 1229 2297 2533 3812 4124 5669 1132
Size: 97 1068 236 1279 312 1545 2229
Fragments arranged by size:
2229 1545 1279 1068 312 236 97
PasI CC'CwG_GG
Cuts at: 1348 2256 3852 1348
Size: 908 1596 4262
Fragments arranged by size:
4262 1596 908
PciI A'CATG_T
Cuts at: 2529 3808 5665 2529
Size: 1279 1857 3630
Fragments arranged by size:
3630 1857 1279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2715 2926 3555 3672 1108
Size: 1607 211 629 117 4202
Fragments arranged by size:
4202 1607 629 211 117
PfoI T'CCnGG_A
Cuts at: 2742 2901 3287 3458 3678 2742
Size: 159 386 171 220 5830
Fragments arranged by size:
5830 386 220 171 159
PmeI GTTT'AAAC
Cuts at: 4070 4070
Size: 6766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4835 4867 6348 6380 4835
Size: 32 1481 32 5221
Fragments arranged by size:
5221 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2548 2746 2548
Size: 198 6568
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6766
PsiI TTA'TAA
Cuts at: 4336 4336
Size: 6766
PspOMI G'GGCC_C
Cuts at: 2398 2398
Size: 6766
PspXI vC'TCGA_Gb
Cuts at: 3470 3470
Size: 6766
PstI C_TGCA'G
Cuts at: 830 2028 2111 2224 3978 4118 830
Size: 1198 83 113 1754 140 3478
Fragments arranged by size:
3478 1754 1198 140 113 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6766
PvuII CAG'CTG
Cuts at: 2557 2557
Size: 6766
SacI G_AGCT'C
Cuts at: 2541 2541
Size: 6766
SalI G'TCGA_C
Cuts at: 1936 4108 1936
Size: 2172 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 3998 2060
Size: 1938 4828
SbfI CC_TGCA'GG
Cuts at: 4118 4118
Size: 6766
ScaI AGT'ACT
Cuts at: 2798 5014 6726 2798
Size: 2216 1712 2838
Fragments arranged by size:
2838 2216 1712
SfcI C'TryA_G
Cuts at: 826 1045 2024 2107 2220 3014 3974 4114 5249
Size: 219 979 83 113 794 960 140 1135
Cuts at: 5249 5930 6121 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 979 960 794 681 219 191
140 113 83
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6766
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6766
SmaI CCC'GGG
Cuts at: 1872 3078 3983 4093 1872
Size: 1206 905 110 4545
Fragments arranged by size:
4545 1206 905 110
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2593 3470 3536 4870 5771 6033
Size: 197 926 650 877 66 1334 901 262
Cuts at: 6033 820
Size: 1553
Fragments arranged by size:
1553 1334 926 901 877 650 262 197
66
SnaBI TAC'GTA
Cuts at: 493 4557 493
Size: 4064 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6766
SphI G_CATG'C
Cuts at: 1132 4124 1132
Size: 2992 3774
SspI AAT'ATT
Cuts at: 5 52 4690 5
Size: 47 4638 2081
Fragments arranged by size:
4638 2081 47
StuI AGG'CCT
Cuts at: 1517 2591 1517
Size: 1074 5692
StyI C'CwwG_G
Cuts at: 513 1065 2065 3549 3759 3841 3934 4205 513
Size: 552 1000 1484 210 82 93 271 3074
Fragments arranged by size:
3074 1484 1000 552 271 210 93 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2918 3087 3809 4793 4810 4963 5148
Size: 1255 1338 169 722 984 17 153 185
Cuts at: 5148 5593 325
Size: 445 1498
Fragments arranged by size:
1498 1338 1255 984 722 445 185 169
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2796 5012 6724
Size: 274 80 33 51 1443 819 2216 1712
Cuts at: 6724 96
Size: 138
Fragments arranged by size:
2216 1712 1443 819 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2219 2251 2744 2776 6726 6758 2219
Size: 32 493 32 3950 32 2227
Fragments arranged by size:
3950 2227 493 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6766
XbaI T'CTAG_A
Cuts at: 4102 4102
Size: 6766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2923 2923
Size: 6766
XhoI C'TCGA_G
Cuts at: 1943 3470 1943
Size: 1527 5239
XmaI C'CCGG_G
Cuts at: 1870 3076 3981 4091 1870
Size: 1206 905 110 4545
Fragments arranged by size:
4545 1206 905 110
XmnI GAAnn'nnTTC
Cuts at: 2097 4895 2097
Size: 2798 3968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4574 276
Size: 53 83 186 936 3040 2468
Fragments arranged by size:
3040 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI