Restriction map for
pFN21AB9521
(Circular) (Six-base) MAPSORT of: pFN21AB9521.seq Check: 4160 from: 1 to: 6469
pFN21AB9521 6469 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2324 2324
Size: 6469
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4279 278
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3812 1937
Size: 1875 4594
Acc65I G'GTAC_C
Cuts at: 2705 2725 3790 2705
Size: 20 1065 5384
Fragments arranged by size:
5384 1065 20
AclI AA'CG_TT
Cuts at: 1563 4596 4969 1563
Size: 3033 373 3063
Fragments arranged by size:
3063 3033 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2536 3438 4532 5916 6184 1632
Size: 904 902 1094 1384 268 1917
Fragments arranged by size:
1917 1384 1094 904 902 268
AfeI AGC'GCT
Cuts at: 2489 2990 2489
Size: 501 5968
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6272
AflIII A'CryG_T
Cuts at: 1203 5368 6345 1203
Size: 4165 977 1327
Fragments arranged by size:
4165 1327 977
AgeI A'CCGG_T
Cuts at: 5300 5300
Size: 6469
AhdI GACnn_n'nnGTC
Cuts at: 5198 5198
Size: 6469
AleI CACnn'nnGTG
Cuts at: 1166 2858 1166
Size: 1692 4777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6469
AlwNI CAG_nnn'CTG
Cuts at: 2407 2620 2956 4212 5784 2407
Size: 213 336 1256 1572 3092
Fragments arranged by size:
3092 1572 1256 336 213
ApaI G_GGCC'C
Cuts at: 2629 3349 3737 2629
Size: 720 388 5361
Fragments arranged by size:
5361 720 388
ApaLI G'TGCA_C
Cuts at: 4526 5682 4526
Size: 1156 5313
ApoI r'AATT_y
Cuts at: 1493 3778 4004 4156 6265 1493
Size: 2285 226 152 2109 1697
Fragments arranged by size:
2285 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5023 160
Size: 872 3991 1606
Fragments arranged by size:
3991 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2751 3273 3350 3794 1870
Size: 73 67 741 522 77 444 4545
Fragments arranged by size:
4545 741 522 444 77 73 67
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2251 2284 2251
Size: 33 6436
BamHI G'GATC_C
Cuts at: 3725 3799 4166 3725
Size: 74 367 6028
Fragments arranged by size:
6028 367 74
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2705 2725 2860 2905 3790
Size: 325 217 627 918 20 135 45 885
Cuts at: 3790 5245 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 918 885 627 325 217 135
45 20
BanII G_rGCy'C
Cuts at: 1391 2079 2629 2644 3349 3737 3788 1391
Size: 688 550 15 705 388 51 4072
Fragments arranged by size:
4072 705 688 550 388 51 15
BbeI G_GCGC'C
Cuts at: 2909 2909
Size: 6469
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2272 928
Size: 971 373 5125
Fragments arranged by size:
5125 971 373
BbvCI CC'TCA_GC
Cuts at: 2111 2384 2111
Size: 273 6196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2213 2247 2263 2297 3560 3594 4660
Size: 34 276 34 16 34 1263 34 1066
Cuts at: 4660 4694 1903
Size: 34 3678
Fragments arranged by size:
3678 1263 1066 276 34 34 34 34
34 16
BciVI GTATCCnnnnn_n'
Cuts at: 4361 5571 4361
Size: 1210 5259
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3175 5080 136
Size: 107 122 71 631 605 1503 1905 1525
Fragments arranged by size:
1905 1525 1503 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6464 6464
Size: 6469
BlpI GC'TnA_GC
Cuts at: 3886 3886
Size: 6469
Bme1580I G_kGCm'C
Cuts at: 1790 2177 2202 2629 2865 3349 3573 3737 4530
Size: 387 25 427 236 484 224 164 793
Cuts at: 4530 5686 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 793 484 427 387 236 224
164 25
BmgBI CAC'GTC
Cuts at: 1318 3004 1318
Size: 1686 4783
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3605 4200 5158
Size: 416 53 83 399 24 2188 595 958
Cuts at: 5158 442
Size: 1753
Fragments arranged by size:
2188 1753 958 595 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6469
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2053 2053
Size: 6469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 5129 1239
Size: 929 2961 2579
Fragments arranged by size:
2961 2579 929
Bpu10I CC'TnA_GC
Cuts at: 2111 2384 2111
Size: 273 6196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2876 3218 4594 5459 5757 2876
Size: 342 1376 865 298 3588
Fragments arranged by size:
3588 1376 865 342 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5132 882
Size: 617 3633 2219
Fragments arranged by size:
3633 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4260 493
Size: 711 3056 2702
Fragments arranged by size:
3056 2702 711
BsaBI GATnn'nnATC
Cuts at: 6463 6463
Size: 6469
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2906 3235 4276 4658
Size: 53 83 186 936 1373 329 1041 382
Cuts at: 4658 5293 275
Size: 635 1451
Fragments arranged by size:
1451 1373 1041 936 635 382 329 186
83 53
BsaWI w'CCGG_w
Cuts at: 4902 5300 5574 5721 6132 4902
Size: 398 274 147 411 5239
Fragments arranged by size:
5239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6244
Fragments arranged by size:
6244 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 3059 3183 3413 3527 3567 1186
Size: 859 1014 124 230 114 40 4088
Fragments arranged by size:
4088 1014 859 230 124 114 40
BseYI C'CCAG_C
Cuts at: 3331 3756 5672 6168 3331
Size: 425 1916 496 3632
Fragments arranged by size:
3632 1916 496 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2243 3278 1163
Size: 573 507 1035 4354
Fragments arranged by size:
4354 1035 573 507
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2328 3919 4680 5708 1540
Size: 275 186 327 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 327 275 186
BsiHKAI G_wGCw'C
Cuts at: 2942 4530 4615 5686 2942
Size: 1588 85 1071 3725
Fragments arranged by size:
3725 1588 1071 85
BsmI GAATG_Cn'
Cuts at: 3980 4073 3980
Size: 93 6376
BsmBI CGTCTCn'nnnn_
Cuts at: 3670 3670
Size: 6469
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2177 2202 2629 2644 2865 2942
Size: 399 289 98 25 427 15 221 77
Cuts at: 2942 3349 3573 3737 3788 4530 4615 5686 1391
Size: 407 224 164 51 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 427 407 399 289 224
221 164 98 85 77 51 25 15
BspEI T'CCGG_A
Cuts at: 6132 6132
Size: 6469
BspHI T'CATG_A
Cuts at: 4358 6088 4358
Size: 1730 4739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2324 2797 3824 844
Size: 1480 473 1027 3489
Fragments arranged by size:
3489 1480 1027 473
BsrBI CCG'CTC
Cuts at: 1394 1823 4356 1394
Size: 429 2533 3507
Fragments arranged by size:
3507 2533 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3029 4964 5138 65
Size: 1183 1781 1935 174 1396
Fragments arranged by size:
1935 1781 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5113 5300 1700
Size: 110 3303 187 2869
Fragments arranged by size:
3303 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6469
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6469
BssSI C'ACGA_G
Cuts at: 4529 5541 4529
Size: 1012 5457
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2407 2411 794
Size: 1613 4 4852
Fragments arranged by size:
4852 1613 4
BstBI TT'CG_AA
Cuts at: 5273 5273
Size: 6469
BstEII G'GTnAC_C
Cuts at: 1184 2809 1184
Size: 1625 4844
BstXI CCAn_nnnn'nTGG
Cuts at: 2740 2872 2740
Size: 132 6337
BstYI r'GATC_y
Cuts at: 1972 3725 3799 4166 4551 4568 6020 6106 6118
Size: 1753 74 367 385 17 1452 86 12
Cuts at: 6118 6464 1972
Size: 346 1977
Fragments arranged by size:
1977 1753 1452 385 367 346 86 74
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2217 2500 2943 3488 513
Size: 552 939 213 283 443 545 3494
Fragments arranged by size:
3494 939 552 545 443 283 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2330 487
Size: 683 56 1104 4626
Fragments arranged by size:
4626 1104 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 2453 3985 4779 4799 2405
Size: 48 1532 794 20 4075
Fragments arranged by size:
4075 1532 794 48 20
ClaI AT'CG_AT
Cuts at: 1554 3531 1554
Size: 1977 4492
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2668 2703 529
Size: 35 2104 35 4295
Fragments arranged by size:
4295 2104 35 35
DraI TTT'AAA
Cuts at: 3773 3773
Size: 6469
DraIII CAC_nnn'GTG
Cuts at: 3015 3015
Size: 6469
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3323 5476 809
Size: 507 2007 2153 1802
Fragments arranged by size:
2153 2007 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2091 2121 2310
Size: 54 1425 50 275 18 261 30 189
Cuts at: 2310 2598 2711 3030 3432 3590 3916 4805 6434
Size: 288 113 319 402 158 326 889 1629
Cuts at: 6434 8
Size: 43
Fragments arranged by size:
1629 1425 889 402 326 319 288 275
261 189 158 113 54 50 43 30
18
EagI C'GGCC_G
Cuts at: 1537 1812 3916 1537
Size: 275 2104 4090
Fragments arranged by size:
4090 2104 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3191 3405 4399 1353
Size: 1838 214 994 3423
Fragments arranged by size:
3423 1838 994 214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5056 5430 5576 387
Size: 1064 138 3467 374 146 1280
Fragments arranged by size:
3467 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2536 3438 4532 5129 5916 6184
Size: 393 536 368 902 1094 597 787 268
Cuts at: 6184 1239
Size: 1524
Fragments arranged by size:
1524 1094 902 787 597 536 393 368
268
EcoO109I rG'GnC_Cy
Cuts at: 2697 3145 3220 3325 3346 3356 3482 3494 3627
Size: 448 75 105 21 10 126 12 133
Cuts at: 3627 3733 2697
Size: 106 5433
Fragments arranged by size:
5433 448 133 126 106 105 75 21
12 10
EcoRI G'AATT_C
Cuts at: 3778 4156 3778
Size: 378 6091
FspI TGC'GCA
Cuts at: 3008 4975 3008
Size: 1967 4502
FspAI rTGC'GCAy
Cuts at: 3008 3008
Size: 6469
HaeII r_GCGC'y
Cuts at: 1678 2087 2309 2491 2829 2909 2992 5616 1678
Size: 409 222 182 338 80 83 2624 2531
Fragments arranged by size:
2624 2531 409 338 222 182 83 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5110 5142 5184 5216 1373
Size: 32 135 32 3538 32 42 32 2626
Fragments arranged by size:
3538 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2374 3813 4059 669
Size: 988 281 436 1439 246 3079
Fragments arranged by size:
3079 1439 988 436 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6469
HpaI GTT'AAC
Cuts at: 4059 4059
Size: 6469
KasI G'GCGC_C
Cuts at: 2905 2905
Size: 6469
KpnI G_GTAC'C
Cuts at: 2709 2729 3794 2709
Size: 20 1065 5384
Fragments arranged by size:
5384 1065 20
MfeI C'AATT_G
Cuts at: 4068 4068
Size: 6469
MluI A'CGCG_T
Cuts at: 6345 6345
Size: 6469
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2073 2271 2877 3369 3578 3628 5583 5767
Size: 768 198 606 492 209 50 1955 184
Cuts at: 5767 1305
Size: 2007
Fragments arranged by size:
2007 1955 768 606 492 209 198 184
50
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2093 2312 2600 3032 3434
Size: 54 1425 343 261 219 288 432 402
Cuts at: 3434 10
Size: 3045
Fragments arranged by size:
3045 1425 432 402 343 288 261 219
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2186 2354 2429 2738 2858 2870 4427
Size: 648 1020 168 75 309 120 12 1557
Cuts at: 4427 4786 4945 518
Size: 359 159 2042
Fragments arranged by size:
2042 1557 1020 648 359 309 168 159
120 75 12
MspA1I CmG'CkG
Cuts at: 1606 2195 2227 2502 3760 3885 4562 5710 5955
Size: 589 32 275 1258 125 677 1148 245
Cuts at: 5955 1606
Size: 2120
Fragments arranged by size:
2120 1258 1148 677 589 275 245 125
32
NarI GG'CG_CC
Cuts at: 2906 2906
Size: 6469
NcoI C'CATG_G
Cuts at: 513 1065 2004 2943 513
Size: 552 939 939 4039
Fragments arranged by size:
4039 939 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6469
NotI GC'GGCC_GC
Cuts at: 3916 3916
Size: 6469
NspI r_CATG'y
Cuts at: 1132 1229 2353 2414 3184 3827 5372 1132
Size: 97 1124 61 770 643 1545 2229
Fragments arranged by size:
2229 1545 1124 770 643 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6469
PciI A'CATG_T
Cuts at: 5368 5368
Size: 6469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2100 2469 3451 1108
Size: 992 369 982 4126
Fragments arranged by size:
4126 992 982 369
PfoI T'CCnGG_A
Cuts at: 3507 3507
Size: 6469
PmeI GTTT'AAAC
Cuts at: 3773 3773
Size: 6469
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4538 4570 6051 6083 4538
Size: 32 1481 32 4924
Fragments arranged by size:
4924 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3145 3220 3325 3356 3482 3494 3627 3145
Size: 75 105 31 126 12 133 5987
Fragments arranged by size:
5987 133 126 105 75 31 12
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6469
PsiI TTA'TAA
Cuts at: 4039 4039
Size: 6469
PspOMI G'GGCC_C
Cuts at: 2625 3345 3733 2625
Size: 720 388 5361
Fragments arranged by size:
5361 720 388
PstI C_TGCA'G
Cuts at: 830 2808 3821 830
Size: 1978 1013 3478
Fragments arranged by size:
3478 1978 1013
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6469
PvuII CAG'CTG
Cuts at: 2195 2227 3760 2195
Size: 32 1533 4904
Fragments arranged by size:
4904 1533 32
SacII CC_GC'GG
Cuts at: 2503 2503
Size: 6469
SalI G'TCGA_C
Cuts at: 1936 3811 1936
Size: 1875 4594
SanDI GG'GwC_CC
Cuts at: 3145 3482 3627 3145
Size: 337 145 5987
Fragments arranged by size:
5987 337 145
SapI GCTCTTCn'nnn_
Cuts at: 3191 3191
Size: 6469
SbfI CC_TGCA'GG
Cuts at: 2808 3821 2808
Size: 1013 5456
ScaI AGT'ACT
Cuts at: 4717 6429 4717
Size: 1712 4757
SfcI C'TryA_G
Cuts at: 826 1045 2804 3817 4952 5633 5824 826
Size: 219 1759 1013 1135 681 191 1471
Fragments arranged by size:
1759 1471 1135 1013 681 219 191
SfoI GGC'GCC
Cuts at: 2907 2907
Size: 6469
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6469
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6469
SmaI CCC'GGG
Cuts at: 1872 3796 1872
Size: 1924 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2891 3197 4573 5474 5736 820
Size: 197 926 948 306 1376 901 262 1553
Fragments arranged by size:
1553 1376 948 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 4260 493
Size: 3767 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6469
SphI G_CATG'C
Cuts at: 1132 2414 3827 1132
Size: 1282 1413 3774
Fragments arranged by size:
3774 1413 1282
SspI AAT'ATT
Cuts at: 5 52 4393 5
Size: 47 4341 2081
Fragments arranged by size:
4341 2081 47
StuI AGG'CCT
Cuts at: 1517 2036 1517
Size: 519 5950
StyI C'CwwG_G
Cuts at: 513 1065 2004 2700 2943 3622 3908 513
Size: 552 939 696 243 679 286 3074
Fragments arranged by size:
3074 939 696 679 552 286 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4496 4513 4666 4851 5296 325
Size: 1255 2916 17 153 185 445 1498
Fragments arranged by size:
2916 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4715 6427 96
Size: 274 80 33 51 1443 2738 1712 138
Fragments arranged by size:
2738 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6429 6461 6429
Size: 32 6437
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6469
XbaI T'CTAG_A
Cuts at: 3805 3805
Size: 6469
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2215 2215
Size: 6469
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6469
XmaI C'CCGG_G
Cuts at: 1870 3794 1870
Size: 1924 4545
XmnI GAAnn'nnTTC
Cuts at: 4598 4598
Size: 6469
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4277 276
Size: 53 83 186 936 2743 2468
Fragments arranged by size:
2743 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI NaeI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SexAI SfiI
SrfI SwaI