Restriction map for
pFN21AB9519
(Circular) (Six-base) MAPSORT of: pFN21AB9519.seq Check: 3097 from: 1 to: 5824
pFN21AB9519 5824 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3634 278
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2613 3167 1937
Size: 676 554 4594
Fragments arranged by size:
4594 676 554
Acc65I G'GTAC_C
Cuts at: 3145 3145
Size: 5824
AclI AA'CG_TT
Cuts at: 1563 3951 4324 1563
Size: 2388 373 3063
Fragments arranged by size:
3063 2388 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2260 3887 5271 5539 1632
Size: 628 1627 1384 268 1917
Fragments arranged by size:
1917 1627 1384 628 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5627
AflIII A'CryG_T
Cuts at: 1203 4723 5700 1203
Size: 3520 977 1327
Fragments arranged by size:
3520 1327 977
AgeI A'CCGG_T
Cuts at: 4655 4655
Size: 5824
AhdI GACnn_n'nnGTC
Cuts at: 4553 4553
Size: 5824
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2305 1184
Size: 1121 4703
AlwNI CAG_nnn'CTG
Cuts at: 3567 5139 3567
Size: 1572 4252
ApaI G_GGCC'C
Cuts at: 2763 2763
Size: 5824
ApaLI G'TGCA_C
Cuts at: 2280 3881 5037 2280
Size: 1601 1156 3067
Fragments arranged by size:
3067 1601 1156
ApoI r'AATT_y
Cuts at: 1493 3133 3359 3511 5620 1493
Size: 1640 226 152 2109 1697
Fragments arranged by size:
2109 1697 1640 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4378 160
Size: 872 3346 1606
Fragments arranged by size:
3346 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
AvaI C'yCGr_G
Cuts at: 1870 1943 2708 3149 1870
Size: 73 765 441 4545
Fragments arranged by size:
4545 765 441 73
BamHI G'GATC_C
Cuts at: 3154 3521 3154
Size: 367 5457
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2492 2671 3089 3145 4600
Size: 325 217 627 705 179 418 56 1455
Cuts at: 4600 618
Size: 1842
Fragments arranged by size:
1842 1455 705 627 418 325 217 179
56
BanII G_rGCy'C
Cuts at: 1391 2763 3143 1391
Size: 1372 380 4072
Fragments arranged by size:
4072 1372 380
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2863 2897 4015 4049 1903
Size: 34 926 34 1118 34 3678
Fragments arranged by size:
3678 1118 926 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2795 3716 4926 2795
Size: 921 1210 3693
Fragments arranged by size:
3693 1210 921
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5824
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2309 4435 136
Size: 107 122 71 631 605 637 2126 1525
Fragments arranged by size:
2126 1525 637 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5819 5819
Size: 5824
BlpI GC'TnA_GC
Cuts at: 3241 3241
Size: 5824
Bme1580I G_kGCm'C
Cuts at: 1790 2161 2284 2495 2763 2958 3083 3094 3885
Size: 371 123 211 268 195 125 11 791
Cuts at: 3885 5041 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 791 371 268 211 195 125
123 11
BmgBI CAC'GTC
Cuts at: 1318 2488 1318
Size: 1170 4654
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2831 3555 4513
Size: 416 53 83 399 24 1414 724 958
Cuts at: 4513 442
Size: 1753
Fragments arranged by size:
1753 1414 958 724 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5824
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2061 2061
Size: 5824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4484 1239
Size: 3245 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2132 3029 3949 4814 5112 2132
Size: 897 920 865 298 2844
Fragments arranged by size:
2844 920 897 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4487 882
Size: 617 2988 2219
Fragments arranged by size:
2988 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3615 493
Size: 711 2411 2702
Fragments arranged by size:
2702 2411 711
BsaBI GATnn'nnATC
Cuts at: 5818 5818
Size: 5824
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3631 4013 4648 275
Size: 53 83 186 936 2098 382 635 1451
Fragments arranged by size:
2098 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2018 4257 4655 4929 5076 5487 2018
Size: 2239 398 274 147 411 2355
Fragments arranged by size:
2355 2239 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2537 1178
Size: 30 165 30 1104 30 4465
Fragments arranged by size:
4465 1104 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2045 2528 2618 2885 1186
Size: 859 483 90 267 4125
Fragments arranged by size:
4125 859 483 267 90
BseYI C'CCAG_C
Cuts at: 2089 2563 2779 2854 3075 5027 5523 2089
Size: 474 216 75 221 1952 496 2390
Fragments arranged by size:
2390 1952 496 474 221 216 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2684 1163
Size: 573 411 537 4303
Fragments arranged by size:
4303 573 537 411
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3274 4035 5063 1540
Size: 275 186 1273 761 1028 2301
Fragments arranged by size:
2301 1273 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2148 2284 2319 2592 3885 3970 5041 2148
Size: 136 35 273 1293 85 1071 2931
Fragments arranged by size:
2931 1293 1071 273 136 85 35
BsmI GAATG_Cn'
Cuts at: 3335 3428 3335
Size: 93 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2161 2284 2319 2495 2592 2763
Size: 399 358 13 123 35 176 97 171
Cuts at: 2763 2958 3083 3094 3143 3885 3970 5041 1391
Size: 195 125 11 49 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 358 195 176 171
125 123 97 85 49 35 13 11
BspEI T'CCGG_A
Cuts at: 5487 5487
Size: 5824
BspHI T'CATG_A
Cuts at: 3713 5443 3713
Size: 1730 4094
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3179 844
Size: 2335 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2040 3711 1394
Size: 429 217 1671 3507
Fragments arranged by size:
3507 1671 429 217
BsrDI GCAATG_nn'
Cuts at: 65 1248 4319 4493 65
Size: 1183 3071 174 1396
Fragments arranged by size:
3071 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2717 2744 2811 4468 4655 1700
Size: 110 907 27 67 1657 187 2869
Fragments arranged by size:
2869 1657 907 187 110 67 27
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5824
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5824
BssSI C'ACGA_G
Cuts at: 3884 4896 3884
Size: 1012 4812
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2671 794
Size: 1877 3947
BstBI TT'CG_AA
Cuts at: 4628 4628
Size: 5824
BstEII G'GTnAC_C
Cuts at: 1184 2901 1184
Size: 1717 4107
BstYI r'GATC_y
Cuts at: 1972 3154 3521 3906 3923 5375 5461 5473 5819
Size: 1182 367 385 17 1452 86 12 346
Cuts at: 5819 1972
Size: 1977
Fragments arranged by size:
1977 1452 1182 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2028 3056 2028
Size: 1028 4796
BtgI C'CryG_G
Cuts at: 513 1065 2004 2257 2463 2701 2755 513
Size: 552 939 253 206 238 54 3582
Fragments arranged by size:
3582 939 552 253 238 206 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2362 2522 487
Size: 683 56 1136 160 3789
Fragments arranged by size:
3789 1136 683 160 56
BtsI GCAGTG_nn'
Cuts at: 2879 2973 3340 4134 4154 2879
Size: 94 367 794 20 4549
Fragments arranged by size:
4549 794 367 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5824
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5789
DraI TTT'AAA
Cuts at: 3128 3128
Size: 5824
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4831 809
Size: 507 3515 1802
Fragments arranged by size:
3515 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2202 2310
Size: 54 1425 50 275 18 177 195 108
Cuts at: 2310 2373 2460 2719 2752 2970 3271 4160 5789
Size: 63 87 259 33 218 301 889 1629
Cuts at: 5789 8
Size: 43
Fragments arranged by size:
1629 1425 889 301 275 259 218 195
177 108 87 63 54 50 43 33
18
EagI C'GGCC_G
Cuts at: 1537 1812 3271 1537
Size: 275 1459 4090
Fragments arranged by size:
4090 1459 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2269 2636 3754 1353
Size: 916 367 1118 3423
Fragments arranged by size:
3423 1118 916 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4411 4785 4931 387
Size: 1064 138 2822 374 146 1280
Fragments arranged by size:
2822 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2260 3887 4484 5271 5539 1239
Size: 393 628 1627 597 787 268 1524
Fragments arranged by size:
1627 1524 787 628 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2165 2165
Size: 5824
EcoO109I rG'GnC_Cy
Cuts at: 3040 3040
Size: 5824
EcoRI G'AATT_C
Cuts at: 3133 3511 3133
Size: 378 5446
FspI TGC'GCA
Cuts at: 4330 4330
Size: 5824
HaeII r_GCGC'y
Cuts at: 1678 2046 4971 1678
Size: 368 2925 2531
Fragments arranged by size:
2925 2531 368
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4465 4497 4539 4571 1373
Size: 32 135 32 2893 32 42 32 2626
Fragments arranged by size:
2893 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3010 3168 3414 669
Size: 988 281 1072 158 246 3079
Fragments arranged by size:
3079 1072 988 281 246 158
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5824
HpaI GTT'AAC
Cuts at: 3414 3414
Size: 5824
KpnI G_GTAC'C
Cuts at: 3149 3149
Size: 5824
MfeI C'AATT_G
Cuts at: 3015 3423 3015
Size: 408 5416
MluI A'CGCG_T
Cuts at: 5700 5700
Size: 5824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 5122 1305
Size: 3633 184 2007
Fragments arranged by size:
3633 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2375 2462 2972 10
Size: 54 1425 343 177 366 87 510 2862
Fragments arranged by size:
2862 1425 510 366 343 177 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2438 2576 3782 4141 4300 518
Size: 648 1272 138 1206 359 159 2042
Fragments arranged by size:
2042 1272 1206 648 359 159 138
MspA1I CmG'CkG
Cuts at: 1606 2289 2854 3240 3917 5065 5310 1606
Size: 683 565 386 677 1148 245 2120
Fragments arranged by size:
2120 1148 683 677 565 386 245
NaeI GCC'GGC
Cuts at: 2746 2813 2746
Size: 67 5757
NcoI C'CATG_G
Cuts at: 513 1065 2004 2463 2701 513
Size: 552 939 459 238 3636
Fragments arranged by size:
3636 939 552 459 238
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5824
NgoMIV G'CCGG_C
Cuts at: 2744 2811 2744
Size: 67 5757
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5824
NotI GC'GGCC_GC
Cuts at: 3271 3271
Size: 5824
NspI r_CATG'y
Cuts at: 1132 1229 2424 2804 2866 3182 4727 1132
Size: 97 1195 380 62 316 1545 2229
Fragments arranged by size:
2229 1545 1195 380 316 97 62
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5824
PciI A'CATG_T
Cuts at: 4723 4723
Size: 5824
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5824
PmeI GTTT'AAAC
Cuts at: 3128 3128
Size: 5824
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3893 3925 5406 5438 3893
Size: 32 1481 32 4279
Fragments arranged by size:
4279 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3040 3040
Size: 5824
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5824
PsiI TTA'TAA
Cuts at: 3394 3394
Size: 5824
PspOMI G'GGCC_C
Cuts at: 2759 2759
Size: 5824
PstI C_TGCA'G
Cuts at: 830 3176 830
Size: 2346 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5824
PvuII CAG'CTG
Cuts at: 2289 2854 2289
Size: 565 5259
SalI G'TCGA_C
Cuts at: 1936 3166 1936
Size: 1230 4594
SapI GCTCTTCn'nnn_
Cuts at: 2269 2269
Size: 5824
SbfI CC_TGCA'GG
Cuts at: 3176 3176
Size: 5824
ScaI AGT'ACT
Cuts at: 4072 5784 4072
Size: 1712 4112
SfcI C'TryA_G
Cuts at: 826 1045 3172 4307 4988 5179 826
Size: 219 2127 1135 681 191 1471
Fragments arranged by size:
2127 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2309 2309
Size: 5824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5824
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5824
SmaI CCC'GGG
Cuts at: 1872 2710 3151 1872
Size: 838 441 4545
Fragments arranged by size:
4545 838 441
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 3044 3928 4829 5091 820
Size: 197 926 204 897 884 901 262 1553
Fragments arranged by size:
1553 926 901 897 884 262 204 197
SnaBI TAC'GTA
Cuts at: 493 3615 493
Size: 3122 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5824
SphI G_CATG'C
Cuts at: 1132 2804 3182 1132
Size: 1672 378 3774
Fragments arranged by size:
3774 1672 378
SspI AAT'ATT
Cuts at: 5 52 3748 5
Size: 47 3696 2081
Fragments arranged by size:
3696 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5824
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 2701 3263 513
Size: 552 939 459 238 562 3074
Fragments arranged by size:
3074 939 562 552 459 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3851 3868 4021 4206 4651 325
Size: 1255 2271 17 153 185 445 1498
Fragments arranged by size:
2271 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4070 5782 96
Size: 274 80 33 51 1443 2093 1712 138
Fragments arranged by size:
2093 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2263 2295 2507 2539 5784 5816 2263
Size: 32 212 32 3245 32 2271
Fragments arranged by size:
3245 2271 212 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2452 2834 1316
Size: 1136 382 4306
Fragments arranged by size:
4306 1136 382
XbaI T'CTAG_A
Cuts at: 3160 3160
Size: 5824
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 2560
Size: 5824
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5824
XmaI C'CCGG_G
Cuts at: 1870 2708 3149 1870
Size: 838 441 4545
Fragments arranged by size:
4545 838 441
XmnI GAAnn'nnTTC
Cuts at: 3953 3953
Size: 5824
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3632 276
Size: 53 83 186 936 2098 2468
Fragments arranged by size:
2468 2098 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PfoI PmlI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfoI SrfI SwaI