Restriction map for
pFN21AB9518
(Circular) (Six-base) MAPSORT of: pFN21AB9518.seq Check: 7470 from: 1 to: 6496
pFN21AB9518 6496 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4306 278
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3839 1937
Size: 1902 4594
Acc65I G'GTAC_C
Cuts at: 3817 3817
Size: 6496
AclI AA'CG_TT
Cuts at: 1563 4623 4996 1563
Size: 3060 373 3063
Fragments arranged by size:
3063 3060 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2145 2255 2882 4559 5943 6211 1632
Size: 473 40 110 627 1677 1384 268 1917
Fragments arranged by size:
1917 1677 1384 627 473 268 110 40
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6299
AflIII A'CryG_T
Cuts at: 1203 5395 6372 1203
Size: 4192 977 1327
Fragments arranged by size:
4192 1327 977
AgeI A'CCGG_T
Cuts at: 5327 5327
Size: 6496
AhdI GACnn_n'nnGTC
Cuts at: 5225 5225
Size: 6496
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6496
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6496
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3054 3086 3054
Size: 32 6464
AlwNI CAG_nnn'CTG
Cuts at: 4239 5811 4239
Size: 1572 4924
ApaLI G'TGCA_C
Cuts at: 4553 5709 4553
Size: 1156 5340
ApoI r'AATT_y
Cuts at: 1493 2981 3101 3286 3397 3557 3805 4031 4183
Size: 1488 120 185 111 160 248 226 152
Cuts at: 4183 6292 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1488 248 226 185 160 152
120 111
AseI AT'TA_AT
Cuts at: 160 1032 5050 160
Size: 872 4018 1606
Fragments arranged by size:
4018 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
AvaI C'yCGr_G
Cuts at: 1870 1943 2639 3821 1870
Size: 73 696 1182 4545
Fragments arranged by size:
4545 1182 696 73
BamHI G'GATC_C
Cuts at: 3826 4193 3826
Size: 367 6129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3817 5272 618
Size: 325 217 627 2030 1455 1842
Fragments arranged by size:
2030 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3204 3756 3815 1391
Size: 1813 552 59 4072
Fragments arranged by size:
4072 1813 552 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2093 2604 3200 3571 928
Size: 971 194 511 596 371 3853
Fragments arranged by size:
3853 971 596 511 371 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4687 4721 1903
Size: 34 2750 34 3678
Fragments arranged by size:
3678 2750 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4388 5598 4388
Size: 1210 5286
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6496
BfrBI ATG'CAT
Cuts at: 3010 3010
Size: 6496
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2032 5107 136
Size: 107 122 71 631 605 360 3075 1525
Fragments arranged by size:
3075 1525 631 605 360 122 107 71
BglII A'GATC_T
Cuts at: 2998 6491 2998
Size: 3493 3003
BlpI GC'TnA_GC
Cuts at: 3913 3913
Size: 6496
Bme1580I G_kGCm'C
Cuts at: 1790 2297 4557 5713 1790
Size: 507 2260 1156 2573
Fragments arranged by size:
2573 2260 1156 507
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6496
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2696 3207 3384
Size: 416 53 83 399 24 1279 511 177
Cuts at: 3384 4227 5185 442
Size: 843 958 1753
Fragments arranged by size:
1753 1279 958 843 511 416 399 177
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6496
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2832 5156 1239
Size: 1593 2324 2579
Fragments arranged by size:
2579 2324 1593
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2715 3696 4621 5486 5784 2715
Size: 981 925 865 298 3427
Fragments arranged by size:
3427 981 925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2309 5159 882
Size: 617 810 2850 2219
Fragments arranged by size:
2850 2219 810 617
BsaAI yAC'GTr
Cuts at: 493 1204 4287 493
Size: 711 3083 2702
Fragments arranged by size:
3083 2702 711
BsaBI GATnn'nnATC
Cuts at: 6490 6490
Size: 6496
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4303 4685 5320 275
Size: 53 83 186 936 2770 382 635 1451
Fragments arranged by size:
2770 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4929 5327 5601 5748 6159 4929
Size: 398 274 147 411 5266
Fragments arranged by size:
5266 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3662 3692 1178
Size: 30 165 30 2259 30 3982
Fragments arranged by size:
3982 2259 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3450 1186
Size: 2264 4232
BseYI C'CCAG_C
Cuts at: 2144 2790 3573 5699 6195 2144
Size: 646 783 2126 496 2445
Fragments arranged by size:
2445 2126 783 646 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5923
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3946 4707 5735 1540
Size: 275 186 1945 761 1028 2301
Fragments arranged by size:
2301 1945 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2818 3756 4557 4642 5713 2818
Size: 938 801 85 1071 3601
Fragments arranged by size:
3601 1071 938 801 85
BsmI GAATG_Cn'
Cuts at: 3557 4007 4100 3557
Size: 450 93 5953
Fragments arranged by size:
5953 450 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2297 2818 3204 3756 3815 4557 4642
Size: 399 507 521 386 552 59 742 85
Cuts at: 4642 5713 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 552 521 507 399 386
85 59
BspEI T'CCGG_A
Cuts at: 6159 6159
Size: 6496
BspHI T'CATG_A
Cuts at: 3582 4385 6115 3582
Size: 803 1730 3963
Fragments arranged by size:
3963 1730 803
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3851 844
Size: 3007 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2338 4383 1394
Size: 429 515 2045 3507
Fragments arranged by size:
3507 2045 515 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4991 5165 65
Size: 1183 3743 174 1396
Fragments arranged by size:
3743 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 5140 5327 1700
Size: 110 249 3081 187 2869
Fragments arranged by size:
3081 2869 249 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6496
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6496
BssSI C'ACGA_G
Cuts at: 4556 5568 4556
Size: 1012 5484
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2462 794
Size: 1668 4828
BstBI TT'CG_AA
Cuts at: 5300 5300
Size: 6496
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6496
BstYI r'GATC_y
Cuts at: 1972 2019 2998 3826 4193 4578 4595 6047 6133
Size: 47 979 828 367 385 17 1452 86
Cuts at: 6133 6145 6491 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 979 828 385 367 346 86
47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2027 2829 3630 513
Size: 552 939 23 802 801 3379
Fragments arranged by size:
3379 939 802 801 552 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5757
Fragments arranged by size:
5757 683 56
BtsI GCAGTG_nn'
Cuts at: 3155 3624 4012 4806 4826 3155
Size: 469 388 794 20 4825
Fragments arranged by size:
4825 794 469 388 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6496
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2550 2585 529
Size: 35 1986 35 4440
Fragments arranged by size:
4440 1986 35 35
DraI TTT'AAA
Cuts at: 3800 3800
Size: 6496
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5503 809
Size: 507 4187 1802
Fragments arranged by size:
4187 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2053 3943 4832
Size: 54 1425 50 275 18 223 1890 889
Cuts at: 4832 6461 8
Size: 1629 43
Fragments arranged by size:
1890 1629 1425 889 275 223 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3943 1537
Size: 275 2131 4090
Fragments arranged by size:
4090 2131 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4426 1353
Size: 3073 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 5457 5603 387
Size: 1064 138 3494 374 146 1280
Fragments arranged by size:
3494 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3754 3754
Size: 6496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2145 2255 2832 2882 4559 5156
Size: 393 473 40 110 577 50 1677 597
Cuts at: 5156 5943 6211 1239
Size: 787 268 1524
Fragments arranged by size:
1677 1524 787 597 577 473 393 268
110 50 40
EcoRI G'AATT_C
Cuts at: 3397 3805 4183 3397
Size: 408 378 5710
Fragments arranged by size:
5710 408 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2670 3464 2670
Size: 794 5702
FspI TGC'GCA
Cuts at: 5002 5002
Size: 6496
HaeII r_GCGC'y
Cuts at: 1678 2694 5643 1678
Size: 1016 2949 2531
Fragments arranged by size:
2949 2531 1016
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5137 5169 5211 5243 1373
Size: 32 135 32 3565 32 42 32 2626
Fragments arranged by size:
3565 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3840 4086 669
Size: 988 281 1902 246 3079
Fragments arranged by size:
3079 1902 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6496
HpaI GTT'AAC
Cuts at: 4086 4086
Size: 6496
KpnI G_GTAC'C
Cuts at: 3821 3821
Size: 6496
MfeI C'AATT_G
Cuts at: 4095 4095
Size: 6496
MluI A'CGCG_T
Cuts at: 6372 6372
Size: 6496
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3100 3457 5610 5794 1305
Size: 1795 357 2153 184 2007
Fragments arranged by size:
2153 2007 1795 357 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4674
Fragments arranged by size:
4674 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3629 3635 4454 4813 4972 518
Size: 648 2463 6 819 359 159 2042
Fragments arranged by size:
2463 2042 819 648 359 159 6
MspA1I CmG'CkG
Cuts at: 1606 2029 3573 3912 4589 5737 5982 1606
Size: 423 1544 339 677 1148 245 2120
Fragments arranged by size:
2120 1544 1148 677 423 339 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2829 3630 513
Size: 552 939 825 801 3379
Fragments arranged by size:
3379 939 825 801 552
NdeI CA'TA_TG
Cuts at: 387 2414 387
Size: 2027 4469
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6496
NotI GC'GGCC_GC
Cuts at: 3943 3943
Size: 6496
NsiI A_TGCA'T
Cuts at: 3012 3012
Size: 6496
NspI r_CATG'y
Cuts at: 1132 1229 2152 3854 5399 1132
Size: 97 923 1702 1545 2229
Fragments arranged by size:
2229 1702 1545 923 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6496
PciI A'CATG_T
Cuts at: 5395 5395
Size: 6496
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2062 3313 1108
Size: 954 1251 4291
Fragments arranged by size:
4291 1251 954
PmeI GTTT'AAAC
Cuts at: 3800 3800
Size: 6496
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4565 4597 6078 6110 4565
Size: 32 1481 32 4951
Fragments arranged by size:
4951 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6496
PsiI TTA'TAA
Cuts at: 3170 4066 3170
Size: 896 5600
PstI C_TGCA'G
Cuts at: 830 3150 3706 3848 830
Size: 2320 556 142 3478
Fragments arranged by size:
3478 2320 556 142
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6496
PvuII CAG'CTG
Cuts at: 3573 3573
Size: 6496
SacI G_AGCT'C
Cuts at: 3756 3756
Size: 6496
SacII CC_GC'GG
Cuts at: 2030 2030
Size: 6496
SalI G'TCGA_C
Cuts at: 1936 3838 1936
Size: 1902 4594
SbfI CC_TGCA'GG
Cuts at: 3848 3848
Size: 6496
ScaI AGT'ACT
Cuts at: 4744 6456 4744
Size: 1712 4784
SexAI A'CCwGG_T
Cuts at: 3352 3352
Size: 6496
SfcI C'TryA_G
Cuts at: 826 1045 3146 3702 3844 4979 5660 5851 826
Size: 219 2101 556 142 1135 681 191 1471
Fragments arranged by size:
2101 1471 1135 681 556 219 191 142
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6496
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6496
SmaI CCC'GGG
Cuts at: 1872 2641 3823 1872
Size: 769 1182 4545
Fragments arranged by size:
4545 1182 769
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2730 3711 4600 5501 5763 820
Size: 197 926 787 981 889 901 262 1553
Fragments arranged by size:
1553 981 926 901 889 787 262 197
SnaBI TAC'GTA
Cuts at: 493 4287 493
Size: 3794 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6496
SphI G_CATG'C
Cuts at: 1132 2152 3854 1132
Size: 1020 1702 3774
Fragments arranged by size:
3774 1702 1020
SspI AAT'ATT
Cuts at: 5 52 2845 4420 5
Size: 47 2793 1575 2081
Fragments arranged by size:
2793 2081 1575 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6496
StyI C'CwwG_G
Cuts at: 513 1065 2004 2829 3630 3666 3935 513
Size: 552 939 825 801 36 269 3074
Fragments arranged by size:
3074 939 825 801 552 269 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2244 4523 4540 4693 4878 5323 325
Size: 1255 664 2279 17 153 185 445 1498
Fragments arranged by size:
2279 1498 1255 664 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2381 3260 3698
Size: 274 80 33 51 1443 404 879 438
Cuts at: 3698 4742 6454 96
Size: 1044 1712 138
Fragments arranged by size:
1712 1443 1044 879 438 404 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6456 6488 6456
Size: 32 6464
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6496
XbaI T'CTAG_A
Cuts at: 3832 3832
Size: 6496
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3314 3314
Size: 6496
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6496
XmaI C'CCGG_G
Cuts at: 1870 2639 3821 1870
Size: 769 1182 4545
Fragments arranged by size:
4545 1182 769
XmnI GAAnn'nnTTC
Cuts at: 2523 4625 2523
Size: 2102 4394
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4304 276
Size: 53 83 186 936 2770 2468
Fragments arranged by size:
2770 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SanDI SapI SfiI SfoI SrfI SwaI