Restriction map for
pFN21AB9515
(Circular) (Six-base) MAPSORT of: pFN21AB9515.seq Check: 3997 from: 1 to: 6238
pFN21AB9515 6238 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2677 2677
Size: 6238
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4048 278
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2039 2464 2946 3581 1937
Size: 102 425 482 635 4594
Fragments arranged by size:
4594 635 482 425 102
Acc65I G'GTAC_C
Cuts at: 2865 3559 2865
Size: 694 5544
AclI AA'CG_TT
Cuts at: 1563 4365 4738 1563
Size: 2802 373 3063
Fragments arranged by size:
3063 2802 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2362 4301 5685 5953 1632
Size: 730 1939 1384 268 1917
Fragments arranged by size:
1939 1917 1384 730 268
AfeI AGC'GCT
Cuts at: 3343 3343
Size: 6238
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6041
AflIII A'CryG_T
Cuts at: 1203 2521 5137 6114 1203
Size: 1318 2616 977 1327
Fragments arranged by size:
2616 1327 1318 977
AgeI A'CCGG_T
Cuts at: 5069 5069
Size: 6238
AhdI GACnn_n'nnGTC
Cuts at: 4967 4967
Size: 6238
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6238
AlwNI CAG_nnn'CTG
Cuts at: 2261 2590 3981 5553 2261
Size: 329 1391 1572 2946
Fragments arranged by size:
2946 1572 1391 329
ApaLI G'TGCA_C
Cuts at: 4295 5451 4295
Size: 1156 5082
ApoI r'AATT_y
Cuts at: 1493 2233 2415 2804 3037 3547 3773 3925 6034
Size: 740 182 389 233 510 226 152 2109
Cuts at: 6034 1493
Size: 1697
Fragments arranged by size:
2109 1697 740 510 389 233 226 182
152
AseI AT'TA_AT
Cuts at: 160 1032 3411 4792 160
Size: 872 2379 1381 1606
Fragments arranged by size:
2379 1606 1381 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
AvaI C'yCGr_G
Cuts at: 1870 1943 3563 1870
Size: 73 1620 4545
Fragments arranged by size:
4545 1620 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2365 2398 2365
Size: 33 6205
BamHI G'GATC_C
Cuts at: 3568 3935 3568
Size: 367 5871
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2865 3559 5014 618
Size: 325 217 627 1078 694 1455 1842
Fragments arranged by size:
1842 1455 1078 694 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2016 2845 3557 1391
Size: 625 829 712 4072
Fragments arranged by size:
4072 829 712 625
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2738 928
Size: 971 839 4428
Fragments arranged by size:
4428 971 839
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4429 4463 1903
Size: 34 2492 34 3678
Fragments arranged by size:
3678 2492 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4130 5340 4130
Size: 1210 5028
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4849 136
Size: 107 122 71 631 605 3177 1525
Fragments arranged by size:
3177 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6233 6233
Size: 6238
BlpI GC'TnA_GC
Cuts at: 3655 3655
Size: 6238
Bme1580I G_kGCm'C
Cuts at: 1790 4299 5455 1790
Size: 2509 1156 2573
Fragments arranged by size:
2573 2509 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3969 4927 442
Size: 416 53 83 399 24 2552 958 1753
Fragments arranged by size:
2552 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2598 3339 4898 1239
Size: 1359 741 1559 2579
Fragments arranged by size:
2579 1559 1359 741
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4363 5228 5526 4363
Size: 865 298 5075
Fragments arranged by size:
5075 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4901 882
Size: 617 3402 2219
Fragments arranged by size:
3402 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4029 493
Size: 711 2825 2702
Fragments arranged by size:
2825 2702 711
BsaBI GATnn'nnATC
Cuts at: 6232 6232
Size: 6238
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3453 4045 4427 5062
Size: 53 83 186 936 1920 592 382 635
Cuts at: 5062 275
Size: 1451
Fragments arranged by size:
1920 1451 936 635 592 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4671 5069 5343 5490 5901 4671
Size: 398 274 147 411 5008
Fragments arranged by size:
5008 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3449 3479 1178
Size: 30 165 30 2046 30 3937
Fragments arranged by size:
3937 2046 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3006 1186
Size: 1820 4418
BseYI C'CCAG_C
Cuts at: 3302 5441 5937 3302
Size: 2139 496 3603
Fragments arranged by size:
3603 2139 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5665
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 3688 4449 5477 1540
Size: 275 186 45 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 3243 4299 4384 5455 3243
Size: 1056 85 1071 4026
Fragments arranged by size:
4026 1071 1056 85
BsmI GAATG_Cn'
Cuts at: 3749 3842 3749
Size: 93 6145
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 2845 3243 3557 4299 4384 5455
Size: 399 226 829 398 314 742 85 1071
Cuts at: 5455 1391
Size: 2174
Fragments arranged by size:
2174 1071 829 742 399 398 314 226
85
BspEI T'CCGG_A
Cuts at: 5901 5901
Size: 6238
BspHI T'CATG_A
Cuts at: 2455 4127 5857 2455
Size: 1672 1730 2836
Fragments arranged by size:
2836 1730 1672
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2677 3593 844
Size: 1833 916 3489
Fragments arranged by size:
3489 1833 916
BsrBI CCG'CTC
Cuts at: 1394 1823 4125 1394
Size: 429 2302 3507
Fragments arranged by size:
3507 2302 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3517 4733 4907 65
Size: 1183 2269 1216 174 1396
Fragments arranged by size:
2269 1396 1216 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2085 4882 5069 1700
Size: 110 275 2797 187 2869
Fragments arranged by size:
2869 2797 275 187 110
BsrGI T'GTAC_A
Cuts at: 96 2874 96
Size: 2778 3460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6238
BssSI C'ACGA_G
Cuts at: 2127 4298 5310 2127
Size: 2171 1012 3055
Fragments arranged by size:
3055 2171 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3170 3334 794
Size: 2376 164 3698
Fragments arranged by size:
3698 2376 164
BstBI TT'CG_AA
Cuts at: 5042 5042
Size: 6238
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6238
BstYI r'GATC_y
Cuts at: 1972 2321 3201 3568 3935 4320 4337 5789 5875
Size: 349 880 367 367 385 17 1452 86
Cuts at: 5875 5887 6233 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 880 385 367 367 349 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2947 2947
Size: 6238
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5686
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5499
Fragments arranged by size:
5499 683 56
BtsI GCAGTG_nn'
Cuts at: 3754 4548 4568 3754
Size: 794 20 5424
Fragments arranged by size:
5424 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6203
DraI TTT'AAA
Cuts at: 2491 3542 2491
Size: 1051 5187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5245 809
Size: 507 3929 1802
Fragments arranged by size:
3929 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3222 3685 4574
Size: 54 1425 50 275 18 1392 463 889
Cuts at: 4574 6203 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1392 889 463 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3685 1537
Size: 275 1873 4090
Fragments arranged by size:
4090 1873 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2169 2351 3243 4168 1353
Size: 816 182 892 925 3423
Fragments arranged by size:
3423 925 892 816 182
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4825 5199 5345 387
Size: 1064 138 3236 374 146 1280
Fragments arranged by size:
3236 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2362 2598 3339 4301 4898 5685 5953
Size: 393 730 236 741 962 597 787 268
Cuts at: 5953 1239
Size: 1524
Fragments arranged by size:
1524 962 787 741 730 597 393 268
236
EcoRI G'AATT_C
Cuts at: 2804 3547 3925 2804
Size: 743 378 5117
Fragments arranged by size:
5117 743 378
EcoRV GAT'ATC
Cuts at: 2137 2638 2692 2137
Size: 501 54 5683
Fragments arranged by size:
5683 501 54
FspI TGC'GCA
Cuts at: 4744 4744
Size: 6238
HaeII r_GCGC'y
Cuts at: 1678 3345 5385 1678
Size: 1667 2040 2531
Fragments arranged by size:
2531 2040 1667
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 3412 3444 4879
Size: 32 135 32 623 32 1185 32 1435
Cuts at: 4879 4911 4953 4985 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1435 1185 623 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2040 2365 3582 3828 669
Size: 988 281 102 325 1217 246 3079
Fragments arranged by size:
3079 1217 988 325 281 246 102
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6238
HpaI GTT'AAC
Cuts at: 3828 3828
Size: 6238
KpnI G_GTAC'C
Cuts at: 2869 3563 2869
Size: 694 5544
MfeI C'AATT_G
Cuts at: 2022 3837 2022
Size: 1815 4423
MluI A'CGCG_T
Cuts at: 2521 6114 2521
Size: 3593 2645
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5352 5536 1305
Size: 4047 184 2007
Fragments arranged by size:
4047 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3224 10
Size: 54 1425 343 1392 3024
Fragments arranged by size:
3024 1425 1392 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4196 4555 4714 518
Size: 648 3030 359 159 2042
Fragments arranged by size:
3030 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3170 3306 3654 4331 5479 5724 1606
Size: 1564 136 348 677 1148 245 2120
Fragments arranged by size:
2120 1564 1148 677 348 245 136
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5686
NdeI CA'TA_TG
Cuts at: 387 2674 387
Size: 2287 3951
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6238
NotI GC'GGCC_GC
Cuts at: 3685 3685
Size: 6238
NspI r_CATG'y
Cuts at: 1132 1229 2875 3298 3596 5141 1132
Size: 97 1646 423 298 1545 2229
Fragments arranged by size:
2229 1646 1545 423 298 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6238
PciI A'CATG_T
Cuts at: 5137 5137
Size: 6238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3074 1108
Size: 1966 4272
PmeI GTTT'AAAC
Cuts at: 3542 3542
Size: 6238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4307 4339 5820 5852 4307
Size: 32 1481 32 4693
Fragments arranged by size:
4693 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6238
PsiI TTA'TAA
Cuts at: 3808 3808
Size: 6238
PstI C_TGCA'G
Cuts at: 830 2965 3175 3590 830
Size: 2135 210 415 3478
Fragments arranged by size:
3478 2135 415 210
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6238
PvuII CAG'CTG
Cuts at: 3170 3170
Size: 6238
SalI G'TCGA_C
Cuts at: 1936 2038 3580 1936
Size: 102 1542 4594
Fragments arranged by size:
4594 1542 102
SbfI CC_TGCA'GG
Cuts at: 3590 3590
Size: 6238
ScaI AGT'ACT
Cuts at: 3312 4486 6198 3312
Size: 1174 1712 3352
Fragments arranged by size:
3352 1712 1174
SfcI C'TryA_G
Cuts at: 826 1045 2782 2961 3171 3586 4721 5402 5593
Size: 219 1737 179 210 415 1135 681 191
Cuts at: 5593 826
Size: 1471
Fragments arranged by size:
1737 1471 1135 681 415 219 210 191
179
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6238
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6238
SmaI CCC'GGG
Cuts at: 1872 3565 1872
Size: 1693 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4342 5243 5505 820
Size: 197 926 2399 901 262 1553
Fragments arranged by size:
2399 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4029 493
Size: 3536 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6238
SphI G_CATG'C
Cuts at: 1132 3298 3596 1132
Size: 2166 298 3774
Fragments arranged by size:
3774 2166 298
SspI AAT'ATT
Cuts at: 5 52 3532 4162 5
Size: 47 3480 630 2081
Fragments arranged by size:
3480 2081 630 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6238
StyI C'CwwG_G
Cuts at: 513 1065 2016 3393 3677 513
Size: 552 951 1377 284 3074
Fragments arranged by size:
3074 1377 951 552 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 2667 3420 4265 4282 4435 4620
Size: 1255 480 607 753 845 17 153 185
Cuts at: 4620 5065 325
Size: 445 1498
Fragments arranged by size:
1498 1255 845 753 607 480 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2874 3310 4484
Size: 274 80 33 51 1443 897 436 1174
Cuts at: 4484 6196 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1174 897 436 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6198 6230 6198
Size: 32 6206
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6238
XbaI T'CTAG_A
Cuts at: 3574 3574
Size: 6238
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6238
XmaI C'CCGG_G
Cuts at: 1870 3563 1870
Size: 1693 4545
XmnI GAAnn'nnTTC
Cuts at: 4367 4367
Size: 6238
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4046 276
Size: 53 83 186 936 2512 2468
Fragments arranged by size:
2512 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI
EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI