Restriction map for
pFN21AB9512
(Circular) (Six-base) MAPSORT of: pFN21AB9512.seq Check: 8724 from: 1 to: 6214
pFN21AB9512 6214 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2098 2516 2825 2999 2098
Size: 418 309 174 5313
Fragments arranged by size:
5313 418 309 174
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4024 278
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3557 1937
Size: 1620 4594
Acc65I G'GTAC_C
Cuts at: 2924 3535 2924
Size: 611 5603
AclI AA'CG_TT
Cuts at: 1563 4341 4714 1563
Size: 2778 373 3063
Fragments arranged by size:
3063 2778 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3062 4277 5661 5929 1632
Size: 1430 1215 1384 268 1917
Fragments arranged by size:
1917 1430 1384 1215 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6017
AflIII A'CryG_T
Cuts at: 1203 2877 5113 6090 1203
Size: 1674 2236 977 1327
Fragments arranged by size:
2236 1674 1327 977
AgeI A'CCGG_T
Cuts at: 5045 5045
Size: 6214
AhdI GACnn_n'nnGTC
Cuts at: 4943 4943
Size: 6214
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6214
AlwNI CAG_nnn'CTG
Cuts at: 2420 3957 5529 2420
Size: 1537 1572 3105
Fragments arranged by size:
3105 1572 1537
ApaI G_GGCC'C
Cuts at: 2199 2468 2199
Size: 269 5945
ApaLI G'TGCA_C
Cuts at: 3139 4271 5427 3139
Size: 1132 1156 3926
Fragments arranged by size:
3926 1156 1132
ApoI r'AATT_y
Cuts at: 1493 3523 3749 3901 6010 1493
Size: 2030 226 152 2109 1697
Fragments arranged by size:
2109 2030 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2237 4768 160
Size: 872 1205 2531 1606
Fragments arranged by size:
2531 1606 1205 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2229 2268 3239 3505 3539 1870
Size: 73 93 193 39 971 266 34 4545
Fragments arranged by size:
4545 971 266 193 93 73 39 34
BamHI G'GATC_C
Cuts at: 3544 3911 3544
Size: 367 5847
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2354 2924 3535 4990 618
Size: 325 217 627 567 570 611 1455 1842
Fragments arranged by size:
1842 1455 627 611 570 567 325 217
BanII G_rGCy'C
Cuts at: 1391 2013 2199 2390 2468 2574 3074 3212 3240
Size: 622 186 191 78 106 500 138 28
Cuts at: 3240 3342 3533 1391
Size: 102 191 4072
Fragments arranged by size:
4072 622 500 191 191 186 138 106
102 78 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5243
BbvCI CC'TCA_GC
Cuts at: 2487 2487
Size: 6214
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3230 3264 4405 4439 1903
Size: 34 1293 34 1141 34 3678
Fragments arranged by size:
3678 1293 1141 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4106 5316 4106
Size: 1210 5004
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6214
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2021 4825 136
Size: 107 122 71 631 605 349 2804 1525
Fragments arranged by size:
2804 1525 631 605 349 122 107 71
BglII A'GATC_T
Cuts at: 6209 6209
Size: 6214
BlpI GC'TnA_GC
Cuts at: 3631 3631
Size: 6214
Bme1580I G_kGCm'C
Cuts at: 1790 2199 2468 3143 4275 5431 1790
Size: 409 269 675 1132 1156 2573
Fragments arranged by size:
2573 1156 1132 675 409 269
BmgBI CAC'GTC
Cuts at: 1318 2698 1318
Size: 1380 4834
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3945 4903 442
Size: 416 53 83 399 24 2528 958 1753
Fragments arranged by size:
2528 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 6214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2166 2430 4874 1239
Size: 927 264 2444 2579
Fragments arranged by size:
2579 2444 927 264
Bpu10I CC'TnA_GC
Cuts at: 2487 3468 2487
Size: 981 5233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2658 4339 5204 5502 2658
Size: 1681 865 298 3370
Fragments arranged by size:
3370 1681 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2738 3052 4877 882
Size: 617 1239 314 1825 2219
Fragments arranged by size:
2219 1825 1239 617 314
BsaAI yAC'GTr
Cuts at: 493 1204 4005 493
Size: 711 2801 2702
Fragments arranged by size:
2801 2702 711
BsaBI GATnn'nnATC
Cuts at: 6208 6208
Size: 6214
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4021 4403 5038 275
Size: 53 83 186 936 2488 382 635 1451
Fragments arranged by size:
2488 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2376 3438 4647 5045 5319 5466 5877 2376
Size: 1062 1209 398 274 147 411 2713
Fragments arranged by size:
2713 1209 1062 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2390 2420 2598 2628 1178
Size: 30 165 30 987 30 178 30 4764
Fragments arranged by size:
4764 987 178 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2111 2114 2229 1186
Size: 925 3 115 5171
Fragments arranged by size:
5171 925 115 3
BseYI C'CCAG_C
Cuts at: 2341 5417 5913 2341
Size: 3076 496 2642
Fragments arranged by size:
3076 2642 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2147 2152 1163
Size: 573 411 5 5225
Fragments arranged by size:
5225 573 411 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2032 3664 4425 5453 1540
Size: 275 186 31 1632 761 1028 2301
Fragments arranged by size:
2301 1632 1028 761 275 186 31
BsiHKAI G_wGCw'C
Cuts at: 2574 3143 4275 4360 5431 2574
Size: 569 1132 85 1071 3357
Fragments arranged by size:
3357 1132 1071 569 85
BsmI GAATG_Cn'
Cuts at: 3253 3366 3725 3818 3253
Size: 113 359 93 5649
Fragments arranged by size:
5649 359 113 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2199 2390 2468 2574 3074 3143
Size: 399 223 186 191 78 106 500 69
Cuts at: 3143 3212 3240 3342 3533 4275 4360 5431 1391
Size: 69 28 102 191 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 399 223 191 191
186 106 102 85 78 69 69 28
BspEI T'CCGG_A
Cuts at: 2376 5877 2376
Size: 3501 2713
BspHI T'CATG_A
Cuts at: 4103 5833 4103
Size: 1730 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2098 2516 2825 2999 3309 3569 844
Size: 1254 418 309 174 310 260 3489
Fragments arranged by size:
3489 1254 418 310 309 260 174
BsrBI CCG'CTC
Cuts at: 1394 1823 4101 1394
Size: 429 2278 3507
Fragments arranged by size:
3507 2278 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4709 4883 65
Size: 1183 3461 174 1396
Fragments arranged by size:
3461 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2015 2351 3454 4858 5045 1700
Size: 110 205 336 1103 1404 187 2869
Fragments arranged by size:
2869 1404 1103 336 205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6214
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6214
BssSI C'ACGA_G
Cuts at: 4274 5286 4274
Size: 1012 5202
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6214
BstBI TT'CG_AA
Cuts at: 5018 5018
Size: 6214
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6214
BstYI r'GATC_y
Cuts at: 1972 3098 3544 3911 4296 4313 5765 5851 5863
Size: 1126 446 367 385 17 1452 86 12
Cuts at: 5863 6209 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1126 446 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2454 2518 513
Size: 552 939 450 64 4209
Fragments arranged by size:
4209 939 552 450 64
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5475
Fragments arranged by size:
5475 683 56
BtsI GCAGTG_nn'
Cuts at: 2164 3730 4524 4544 2164
Size: 1566 794 20 3834
Fragments arranged by size:
3834 1566 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6214
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2748 2783 529
Size: 35 2184 35 3960
Fragments arranged by size:
3960 2184 35 35
DraI TTT'AAA
Cuts at: 3518 3518
Size: 6214
DraIII CAC_nnn'GTG
Cuts at: 2497 2497
Size: 6214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3044 5221 809
Size: 507 1728 2177 1802
Fragments arranged by size:
2177 1802 1728 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2029 2154 3661
Size: 54 1425 50 275 18 199 125 1507
Cuts at: 3661 4550 6179 8
Size: 889 1629 43
Fragments arranged by size:
1629 1507 1425 889 275 199 125 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2029 3661 1537
Size: 275 217 1632 4090
Fragments arranged by size:
4090 1632 275 217
EarI CTCTTCn'nnn_
Cuts at: 1353 2724 4144 1353
Size: 1371 1420 3423
Fragments arranged by size:
3423 1420 1371
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4801 5175 5321 387
Size: 1064 138 3212 374 146 1280
Fragments arranged by size:
3212 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2572 2572
Size: 6214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2166 2430 3062 4277 4874 5661 5929
Size: 393 534 264 632 1215 597 787 268
Cuts at: 5929 1239
Size: 1524
Fragments arranged by size:
1524 1215 787 632 597 534 393 268
264
EcoNI CCTnn'n_nnAGG
Cuts at: 3305 3305
Size: 6214
EcoO109I rG'GnC_Cy
Cuts at: 2195 2465 2713 3502 2195
Size: 270 248 789 4907
Fragments arranged by size:
4907 789 270 248
EcoRI G'AATT_C
Cuts at: 3523 3901 3523
Size: 378 5836
EcoRV GAT'ATC
Cuts at: 3280 3280
Size: 6214
FspI TGC'GCA
Cuts at: 4720 4720
Size: 6214
HaeII r_GCGC'y
Cuts at: 1678 5361 1678
Size: 3683 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2390 2422 2600 2632 2654
Size: 32 135 32 818 32 178 32 22
Cuts at: 2654 2686 4855 4887 4929 4961 1373
Size: 32 2169 32 42 32 2626
Fragments arranged by size:
2626 2169 818 178 135 42 32 32
32 32 32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3558 3804 669
Size: 988 281 1620 246 3079
Fragments arranged by size:
3079 1620 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6214
HpaI GTT'AAC
Cuts at: 3804 3804
Size: 6214
KpnI G_GTAC'C
Cuts at: 2928 3539 2928
Size: 611 5603
MfeI C'AATT_G
Cuts at: 3813 3813
Size: 6214
MluI A'CGCG_T
Cuts at: 6090 6090
Size: 6214
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2844 3372 5328 5512 1305
Size: 1539 528 1956 184 2007
Fragments arranged by size:
2007 1956 1539 528 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4392
Fragments arranged by size:
4392 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2973 4172 4531 4690 518
Size: 648 1807 1199 359 159 2042
Fragments arranged by size:
2042 1807 1199 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2144 2854 3020 3086 3630 4307 5455
Size: 438 100 710 166 66 544 677 1148
Cuts at: 5455 5700 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 710 677 544 438 245 166
100 66
NaeI GCC'GGC
Cuts at: 2017 2353 3456 2017
Size: 336 1103 4775
Fragments arranged by size:
4775 1103 336
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4723
Fragments arranged by size:
4723 939 552
NdeI CA'TA_TG
Cuts at: 387 3286 387
Size: 2899 3315
NgoMIV G'CCGG_C
Cuts at: 2015 2351 3454 2015
Size: 336 1103 4775
Fragments arranged by size:
4775 1103 336
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6214
NotI GC'GGCC_GC
Cuts at: 3661 3661
Size: 6214
NspI r_CATG'y
Cuts at: 1132 1229 3572 5117 1132
Size: 97 2343 1545 2229
Fragments arranged by size:
2343 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3205 1348
Size: 1857 4357
PciI A'CATG_T
Cuts at: 5113 5113
Size: 6214
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2205 1108
Size: 1097 5117
PfoI T'CCnGG_A
Cuts at: 2882 2882
Size: 6214
PmeI GTTT'AAAC
Cuts at: 3518 3518
Size: 6214
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4283 4315 5796 5828 4283
Size: 32 1481 32 4669
Fragments arranged by size:
4669 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2713 3502 2713
Size: 789 5425
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6214
PsiI TTA'TAA
Cuts at: 3784 3784
Size: 6214
PspOMI G'GGCC_C
Cuts at: 2195 2464 2195
Size: 269 5945
PstI C_TGCA'G
Cuts at: 830 2109 3566 830
Size: 1279 1457 3478
Fragments arranged by size:
3478 1457 1279
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6214
PvuII CAG'CTG
Cuts at: 2144 2854 2144
Size: 710 5504
SacI G_AGCT'C
Cuts at: 2574 2574
Size: 6214
SalI G'TCGA_C
Cuts at: 1936 3556 1936
Size: 1620 4594
SanDI GG'GwC_CC
Cuts at: 2713 2713
Size: 6214
SbfI CC_TGCA'GG
Cuts at: 3566 3566
Size: 6214
ScaI AGT'ACT
Cuts at: 4462 6174 4462
Size: 1712 4502
SexAI A'CCwGG_T
Cuts at: 2802 2858 2802
Size: 56 6158
SfcI C'TryA_G
Cuts at: 826 1045 2105 2620 2849 3562 4697 5378 5569
Size: 219 1060 515 229 713 1135 681 191
Cuts at: 5569 826
Size: 1471
Fragments arranged by size:
1471 1135 1060 713 681 515 229 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6214
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6214
SmaI CCC'GGG
Cuts at: 1872 2231 2270 3541 1872
Size: 359 39 1271 4545
Fragments arranged by size:
4545 1271 359 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2673 4318 5219 5481 820
Size: 197 926 730 1645 901 262 1553
Fragments arranged by size:
1645 1553 926 901 730 262 197
SnaBI TAC'GTA
Cuts at: 493 4005 493
Size: 3512 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6214
SphI G_CATG'C
Cuts at: 1132 3572 1132
Size: 2440 3774
SspI AAT'ATT
Cuts at: 5 52 4138 5
Size: 47 4086 2081
Fragments arranged by size:
4086 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6214
StyI C'CwwG_G
Cuts at: 513 1065 2004 2640 2766 3306 3653 513
Size: 552 939 636 126 540 347 3074
Fragments arranged by size:
3074 939 636 552 540 347 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2545 2776 3119 4241 4258 4411 4596
Size: 1255 965 231 343 1122 17 153 185
Cuts at: 4596 5041 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1122 965 445 343 231 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4460 6172 96
Size: 274 80 33 51 1443 2483 1712 138
Fragments arranged by size:
2483 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2598 2630 6174 6206 2598
Size: 32 3544 32 2606
Fragments arranged by size:
3544 2606 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3425 1316
Size: 2109 4105
XbaI T'CTAG_A
Cuts at: 3550 3550
Size: 6214
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6214
XmaI C'CCGG_G
Cuts at: 1870 2229 2268 3539 1870
Size: 359 39 1271 4545
Fragments arranged by size:
4545 1271 359 39
XmnI GAAnn'nnTTC
Cuts at: 2552 4343 2552
Size: 1791 4423
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4022 276
Size: 53 83 186 936 2488 2468
Fragments arranged by size:
2488 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I FalI FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SapI SfiI SfoI SrfI SwaI XcmI