Restriction map for
pFN21AB9511
(Circular) (Six-base) MAPSORT of: pFN21AB9511.seq Check: 7708 from: 1 to: 5863
pFN21AB9511 5863 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3673 278
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2211 3206 1937
Size: 274 995 4594
Fragments arranged by size:
4594 995 274
Acc65I G'GTAC_C
Cuts at: 2515 3184 2515
Size: 669 5194
AclI AA'CG_TT
Cuts at: 1563 3990 4363 1563
Size: 2427 373 3063
Fragments arranged by size:
3063 2427 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2394 3105 3926 5310 5578 1632
Size: 762 711 821 1384 268 1917
Fragments arranged by size:
1917 1384 821 762 711 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5666
AflIII A'CryG_T
Cuts at: 1203 4762 5739 1203
Size: 3559 977 1327
Fragments arranged by size:
3559 1327 977
AgeI A'CCGG_T
Cuts at: 4694 4694
Size: 5863
AhdI GACnn_n'nnGTC
Cuts at: 4592 4592
Size: 5863
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5863
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3135 1184
Size: 1951 3912
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 5831
AlwNI CAG_nnn'CTG
Cuts at: 3606 5178 3606
Size: 1572 4291
ApaI G_GGCC'C
Cuts at: 2108 2108
Size: 5863
ApaLI G'TGCA_C
Cuts at: 3920 5076 3920
Size: 1156 4707
ApoI r'AATT_y
Cuts at: 1493 2590 3172 3398 3550 5659 1493
Size: 1097 582 226 152 2109 1697
Fragments arranged by size:
2109 1697 1097 582 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4417 160
Size: 872 3385 1606
Fragments arranged by size:
3385 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
AvaI C'yCGr_G
Cuts at: 1870 1943 3188 1870
Size: 73 1245 4545
Fragments arranged by size:
4545 1245 73
BamHI G'GATC_C
Cuts at: 3193 3560 3193
Size: 367 5496
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2515 3184 4639 618
Size: 325 217 627 728 669 1455 1842
Fragments arranged by size:
1842 1455 728 669 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2108 3182 1391
Size: 717 1074 4072
Fragments arranged by size:
4072 1074 717
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4892
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2014 2048 4054 4088 1903
Size: 34 77 34 2006 34 3678
Fragments arranged by size:
3678 2006 77 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2191 3755 4965 2191
Size: 1564 1210 3089
Fragments arranged by size:
3089 1564 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4474 136
Size: 107 122 71 631 605 337 2465 1525
Fragments arranged by size:
2465 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2377 2989 5858 2377
Size: 612 2869 2382
Fragments arranged by size:
2869 2382 612
BlpI GC'TnA_GC
Cuts at: 3280 3280
Size: 5863
Bme1580I G_kGCm'C
Cuts at: 1790 2108 3924 5080 1790
Size: 318 1816 1156 2573
Fragments arranged by size:
2573 1816 1156 318
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5863
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2905 3034 3594
Size: 416 53 83 399 24 1488 129 560
Cuts at: 3594 4552 442
Size: 958 1753
Fragments arranged by size:
1753 1488 958 560 416 399 129 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5863
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2648 2696 2884 4523 1239
Size: 1409 48 188 1639 2579
Fragments arranged by size:
2579 1639 1409 188 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3988 4853 5151 3988
Size: 865 298 4700
Fragments arranged by size:
4700 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2831 4526 882
Size: 617 1332 1695 2219
Fragments arranged by size:
2219 1695 1332 617
BsaAI yAC'GTr
Cuts at: 493 1204 3654 493
Size: 711 2450 2702
Fragments arranged by size:
2702 2450 711
BsaBI GATnn'nnATC
Cuts at: 2988 5857 2988
Size: 2869 2994
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3670 4052 4687 275
Size: 53 83 186 936 2137 382 635 1451
Fragments arranged by size:
2137 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4296 4694 4968 5115 5526 4296
Size: 398 274 147 411 4633
Fragments arranged by size:
4633 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5638
Fragments arranged by size:
5638 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2271 2436 1186
Size: 1085 165 4613
Fragments arranged by size:
4613 1085 165
BseYI C'CCAG_C
Cuts at: 5066 5562 5066
Size: 496 5367
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5290
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2038 3313 4074 5102 1540
Size: 275 186 37 1275 761 1028 2301
Fragments arranged by size:
2301 1275 1028 761 275 186 37
BsiHKAI G_wGCw'C
Cuts at: 3924 4009 5080 3924
Size: 85 1071 4707
Fragments arranged by size:
4707 1071 85
BsmI GAATG_Cn'
Cuts at: 2638 3374 3467 2638
Size: 736 93 5034
Fragments arranged by size:
5034 736 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2108 3182 3924 4009 5080 1391
Size: 399 318 1074 742 85 1071 2174
Fragments arranged by size:
2174 1074 1071 742 399 318 85
BspEI T'CCGG_A
Cuts at: 5526 5526
Size: 5863
BspHI T'CATG_A
Cuts at: 3752 5482 3752
Size: 1730 4133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3019 3218 844
Size: 2175 199 3489
Fragments arranged by size:
3489 2175 199
BsrBI CCG'CTC
Cuts at: 1394 1823 2085 3750 1394
Size: 429 262 1665 3507
Fragments arranged by size:
3507 1665 429 262
BsrDI GCAATG_nn'
Cuts at: 65 1248 2419 4358 4532 65
Size: 1183 1171 1939 174 1396
Fragments arranged by size:
1939 1396 1183 1171 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4507 4694 1700
Size: 110 2697 187 2869
Fragments arranged by size:
2869 2697 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5863
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5863
BssSI C'ACGA_G
Cuts at: 3923 4935 3923
Size: 1012 4851
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5863
BstBI TT'CG_AA
Cuts at: 2668 4667 2668
Size: 1999 3864
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5863
BstXI CCAn_nnnn'nTGG
Cuts at: 2862 2862
Size: 5863
BstYI r'GATC_y
Cuts at: 1972 2335 2377 2989 3193 3560 3945 3962 5414
Size: 363 42 612 204 367 385 17 1452
Cuts at: 5414 5500 5512 5858 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 612 385 367 363 346 204
86 42 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2160 513
Size: 552 1095 4216
Fragments arranged by size:
4216 1095 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5124
Fragments arranged by size:
5124 683 56
BtsI GCAGTG_nn'
Cuts at: 3379 4173 4193 3379
Size: 794 20 5049
Fragments arranged by size:
5049 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5828
DraI TTT'AAA
Cuts at: 3167 3167
Size: 5863
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4870 809
Size: 507 3554 1802
Fragments arranged by size:
3554 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2035 2659 2701
Size: 54 1425 50 275 18 205 624 42
Cuts at: 2701 3001 3046 3310 4199 5828 8
Size: 300 45 264 889 1629 43
Fragments arranged by size:
1629 1425 889 624 300 275 264 205
54 50 45 43 42 18
EagI C'GGCC_G
Cuts at: 1537 1812 2035 3310 1537
Size: 275 223 1275 4090
Fragments arranged by size:
4090 1275 275 223
EarI CTCTTCn'nnn_
Cuts at: 1353 2648 3793 1353
Size: 1295 1145 3423
Fragments arranged by size:
3423 1295 1145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4450 4824 4970 387
Size: 1064 138 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2394 2648 2696 2884 3105 3926 4523
Size: 393 762 254 48 188 221 821 597
Cuts at: 4523 5310 5578 1239
Size: 787 268 1524
Fragments arranged by size:
1524 821 787 762 597 393 268 254
221 188 48
EcoO109I rG'GnC_Cy
Cuts at: 2105 2437 3031 2105
Size: 332 594 4937
Fragments arranged by size:
4937 594 332
EcoRI G'AATT_C
Cuts at: 3172 3550 3172
Size: 378 5485
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2140 2140
Size: 5863
FspI TGC'GCA
Cuts at: 4369 4369
Size: 5863
HaeII r_GCGC'y
Cuts at: 1678 5010 1678
Size: 3332 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2656 2688 4504 4536 4578
Size: 32 135 32 1084 32 1816 32 42
Cuts at: 4578 4610 1373
Size: 32 2626
Fragments arranged by size:
2626 1816 1084 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3207 3453 669
Size: 988 281 1269 246 3079
Fragments arranged by size:
3079 1269 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5863
HpaI GTT'AAC
Cuts at: 3453 3453
Size: 5863
KpnI G_GTAC'C
Cuts at: 2519 3188 2519
Size: 669 5194
MfeI C'AATT_G
Cuts at: 3462 3462
Size: 5863
MluI A'CGCG_T
Cuts at: 5739 5739
Size: 5863
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2532 4977 5161 1305
Size: 1227 2445 184 2007
Fragments arranged by size:
2445 2007 1227 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2661 2703 3003 3048 10
Size: 54 1425 343 829 42 300 45 2825
Fragments arranged by size:
2825 1425 829 343 300 54 45 42
MslI CAynn'nnrTG
Cuts at: 518 1166 3063 3821 4180 4339 518
Size: 648 1897 758 359 159 2042
Fragments arranged by size:
2042 1897 758 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2070 3279 3956 5104 5349 1606
Size: 464 1209 677 1148 245 2120
Fragments arranged by size:
2120 1209 1148 677 464 245
NcoI C'CATG_G
Cuts at: 513 1065 2160 513
Size: 552 1095 4216
Fragments arranged by size:
4216 1095 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5863
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5863
NotI GC'GGCC_GC
Cuts at: 3310 3310
Size: 5863
NspI r_CATG'y
Cuts at: 1132 1229 2029 2587 3221 4766 1132
Size: 97 800 558 634 1545 2229
Fragments arranged by size:
2229 1545 800 634 558 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5863
PciI A'CATG_T
Cuts at: 4762 4762
Size: 5863
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5863
PmeI GTTT'AAAC
Cuts at: 3167 3167
Size: 5863
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3932 3964 5445 5477 3932
Size: 32 1481 32 4318
Fragments arranged by size:
4318 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2437 3031 2437
Size: 594 5269
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5863
PsiI TTA'TAA
Cuts at: 3433 3433
Size: 5863
PspOMI G'GGCC_C
Cuts at: 2104 2104
Size: 5863
PstI C_TGCA'G
Cuts at: 830 2389 3215 830
Size: 1559 826 3478
Fragments arranged by size:
3478 1559 826
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5863
SalI G'TCGA_C
Cuts at: 1936 3205 1936
Size: 1269 4594
SbfI CC_TGCA'GG
Cuts at: 3215 3215
Size: 5863
ScaI AGT'ACT
Cuts at: 4111 5823 4111
Size: 1712 4151
SexAI A'CCwGG_T
Cuts at: 2511 2511
Size: 5863
SfcI C'TryA_G
Cuts at: 826 1045 2385 2751 3211 4346 5027 5218 826
Size: 219 1340 366 460 1135 681 191 1471
Fragments arranged by size:
1471 1340 1135 681 460 366 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5863
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5863
SmaI CCC'GGG
Cuts at: 1872 3190 1872
Size: 1318 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3967 4868 5130 820
Size: 197 926 2024 901 262 1553
Fragments arranged by size:
2024 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3654 493
Size: 3161 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5863
SphI G_CATG'C
Cuts at: 1132 2029 2587 3221 1132
Size: 897 558 634 3774
Fragments arranged by size:
3774 897 634 558
SspI AAT'ATT
Cuts at: 5 52 3787 5
Size: 47 3735 2081
Fragments arranged by size:
3735 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5863
StyI C'CwwG_G
Cuts at: 513 1065 2138 2160 3302 513
Size: 552 1073 22 1142 3074
Fragments arranged by size:
3074 1142 1073 552 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2465 3890 3907 4060 4245 4690 325
Size: 1255 885 1425 17 153 185 445 1498
Fragments arranged by size:
1498 1425 1255 885 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4109 5821 96
Size: 274 80 33 51 1443 2132 1712 138
Fragments arranged by size:
2132 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5823 5855 5823
Size: 32 5831
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5863
XbaI T'CTAG_A
Cuts at: 3199 3199
Size: 5863
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2098 2098
Size: 5863
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5863
XmaI C'CCGG_G
Cuts at: 1870 3188 1870
Size: 1318 4545
XmnI GAAnn'nnTTC
Cuts at: 3992 3992
Size: 5863
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3671 276
Size: 53 83 186 936 2137 2468
Fragments arranged by size:
2468 2137 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI