Restriction map for
pFN21AB9510
(Circular) (Six-base) MAPSORT of: pFN21AB9510.seq Check: 201 from: 1 to: 5821
pFN21AB9510 5821 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3631 278
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3164 1937
Size: 1227 4594
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3948 4321 1563
Size: 2385 373 3063
Fragments arranged by size:
3063 2385 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 3884 5268 5536 1632
Size: 483 1769 1384 268 1917
Fragments arranged by size:
1917 1769 1384 483 268
AflII C'TTAA_G
Cuts at: 820 1017 2810 820
Size: 197 1793 3831
Fragments arranged by size:
3831 1793 197
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 2113 1166
Size: 947 4874
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5821
AlwNI CAG_nnn'CTG
Cuts at: 2408 2837 3564 5136 2408
Size: 429 727 1572 3093
Fragments arranged by size:
3093 1572 727 429
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 3146 1870
Size: 73 1203 4545
Fragments arranged by size:
4545 1203 73
AvrII C'CTAG_G
Cuts at: 2138 2138
Size: 5821
BamHI G'GATC_C
Cuts at: 3151 3518 3151
Size: 367 5454
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3044 3142 4597 618
Size: 325 217 627 1257 98 1455 1842
Fragments arranged by size:
1842 1455 1257 627 325 217 98
BanII G_rGCy'C
Cuts at: 1391 3140 1391
Size: 1749 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4432 136
Size: 107 122 71 631 605 2760 1525
Fragments arranged by size:
2760 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 2072 2346 3238 2072
Size: 274 892 4655
Fragments arranged by size:
4655 892 274
Bme1580I G_kGCm'C
Cuts at: 1790 3882 5038 1790
Size: 2092 1156 2573
Fragments arranged by size:
2573 2092 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2670 3088 4481 1239
Size: 1431 418 1393 2579
Fragments arranged by size:
2579 1431 1393 418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2115 2764 3612 493
Size: 711 911 649 848 2702
Fragments arranged by size:
2702 911 848 711 649
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3628 4010 4645 275
Size: 53 83 186 936 2095 382 635 1451
Fragments arranged by size:
2095 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4254 4652 4926 5073 5484 4254
Size: 398 274 147 411 4591
Fragments arranged by size:
4591 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5596
Fragments arranged by size:
5596 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2240 5024 5520 2240
Size: 2784 496 2541
Fragments arranged by size:
2784 2541 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5248
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3882 3967 5038 3882
Size: 85 1071 4665
Fragments arranged by size:
4665 1071 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3140 3882 3967 5038 1391
Size: 399 1350 742 85 1071 2174
Fragments arranged by size:
2174 1350 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5484 5484
Size: 5821
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2407 3176 844
Size: 1563 769 3489
Fragments arranged by size:
3489 1563 769
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2787 4316 4490 65
Size: 1183 1539 1529 174 1396
Fragments arranged by size:
1539 1529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4465 4652 1700
Size: 110 2655 187 2869
Fragments arranged by size:
2869 2655 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5821
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2975 794
Size: 2181 3640
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstXI CCAn_nnnn'nTGG
Cuts at: 2184 2184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2199 3151 3518 3903 3920 5372 5458 5470
Size: 227 952 367 385 17 1452 86 12
Cuts at: 5470 5816 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 952 385 367 346 227 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2457 2457
Size: 5821
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5269
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 2382 3337 4131 4151 2382
Size: 955 794 20 4052
Fragments arranged by size:
4052 955 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2704 2739 529
Size: 35 2140 35 3611
Fragments arranged by size:
3611 2140 35 35
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4828 809
Size: 507 3512 1802
Fragments arranged by size:
3512 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3268 4157 5786
Size: 54 1425 50 275 18 1438 889 1629
Cuts at: 5786 8
Size: 43
Fragments arranged by size:
1629 1438 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2556 3751 1353
Size: 1203 1195 3423
Fragments arranged by size:
3423 1203 1195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4408 4782 4928 387
Size: 1064 138 2819 374 146 1280
Fragments arranged by size:
2819 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2670 3088 3884 4481 5268 5536
Size: 393 483 555 418 796 597 787 268
Cuts at: 5536 1239
Size: 1524
Fragments arranged by size:
1524 796 787 597 555 483 418 393
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 5821
EcoO109I rG'GnC_Cy
Cuts at: 2362 2362
Size: 5821
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 4968 1678
Size: 3290 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4462 4494 4536 4568 1373
Size: 32 135 32 2890 32 42 32 2626
Fragments arranged by size:
2890 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2484 3165 3411 669
Size: 988 281 72 474 681 246 3079
Fragments arranged by size:
3079 988 681 474 281 246 72
HindIII A'AGCT_T
Cuts at: 748 3094 748
Size: 2346 3475
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4935 5119 1305
Size: 3630 184 2007
Fragments arranged by size:
3630 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3999
Fragments arranged by size:
3999 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2113 2766 2868 3779 4138 4297 518
Size: 648 947 653 102 911 359 159 2042
Fragments arranged by size:
2042 947 911 653 648 359 159 102
MspA1I CmG'CkG
Cuts at: 1606 2015 2350 2408 3237 3914 5062 5307 1606
Size: 409 335 58 829 677 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 409 335 245 58
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5269
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5821
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PmlI CAC'GTG
Cuts at: 2115 2115
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2362 2362
Size: 5821
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2979 3011 2979
Size: 32 5789
PstI C_TGCA'G
Cuts at: 830 2091 2404 3028 3173 830
Size: 1261 313 624 145 3478
Fragments arranged by size:
3478 1261 624 313 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2015 2350 2408 2015
Size: 335 58 5428
Fragments arranged by size:
5428 335 58
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 3022 4069 5781 3022
Size: 1047 1712 3062
Fragments arranged by size:
3062 1712 1047
SexAI A'CCwGG_T
Cuts at: 2049 2049
Size: 5821
SfcI C'TryA_G
Cuts at: 826 1045 2087 2400 3024 3169 4304 4985 5176
Size: 219 1042 313 624 145 1135 681 191
Cuts at: 5176 826
Size: 1471
Fragments arranged by size:
1471 1135 1042 681 624 313 219 191
145
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2810 3925 4826 5088 820
Size: 197 926 867 1115 901 262 1553
Fragments arranged by size:
1553 1115 926 901 867 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2138 2487 3260 513
Size: 552 1073 349 773 3074
Fragments arranged by size:
3074 1073 773 552 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2125 2254 3848 3865 4018 4203 4648
Size: 1255 545 129 1594 17 153 185 445
Cuts at: 4648 325
Size: 1498
Fragments arranged by size:
1594 1498 1255 545 445 185 153 129
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2367 3020 4067
Size: 274 80 33 51 1443 390 653 1047
Cuts at: 4067 5779 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1047 653 390 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5781 5813 5781
Size: 32 5789
Tth111I GACn'n_nGTC
Cuts at: 1316 2939 1316
Size: 1623 4198
XbaI T'CTAG_A
Cuts at: 2497 3157 2497
Size: 660 5161
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2275 2275
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 3950 3950
Size: 5821
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3629 276
Size: 53 83 186 936 2095 2468
Fragments arranged by size:
2468 2095 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspOMI PspXI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI