Restriction map for
pFN21AB9500
(Circular) (Six-base) MAPSORT of: pFN21AB9500.seq Check: 7174 from: 1 to: 6730
pFN21AB9500 6730 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4540 278
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4073 1937
Size: 2136 4594
Acc65I G'GTAC_C
Cuts at: 4009 4051 4009
Size: 42 6688
AclI AA'CG_TT
Cuts at: 1563 4857 5230 1563
Size: 3294 373 3063
Fragments arranged by size:
3294 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3879 4793 6177 6445 1632
Size: 2247 914 1384 268 1917
Fragments arranged by size:
2247 1917 1384 914 268
AflII C'TTAA_G
Cuts at: 820 1017 2264 820
Size: 197 1247 5286
Fragments arranged by size:
5286 1247 197
AflIII A'CryG_T
Cuts at: 1203 5629 6606 1203
Size: 4426 977 1327
Fragments arranged by size:
4426 1327 977
AgeI A'CCGG_T
Cuts at: 5561 5561
Size: 6730
AhdI GACnn_n'nnGTC
Cuts at: 2965 5459 2965
Size: 2494 4236
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6730
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2889 2921 2889
Size: 32 6698
AlwNI CAG_nnn'CTG
Cuts at: 4473 6045 4473
Size: 1572 5158
ApaLI G'TGCA_C
Cuts at: 2163 4787 5943 2163
Size: 2624 1156 2950
Fragments arranged by size:
2950 2624 1156
ApoI r'AATT_y
Cuts at: 1493 2280 2928 3653 3664 3763 3937 4039 4265
Size: 787 648 725 11 99 174 102 226
Cuts at: 4265 4417 6526 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 787 725 648 226 174 152
102 99 11
AseI AT'TA_AT
Cuts at: 160 1032 2916 5284 160
Size: 872 1884 2368 1606
Fragments arranged by size:
2368 1884 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
AvaI C'yCGr_G
Cuts at: 1870 1943 4055 1870
Size: 73 2112 4545
Fragments arranged by size:
4545 2112 73
BamHI G'GATC_C
Cuts at: 4060 4427 4060
Size: 367 6363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 4009 4051 5506 618
Size: 325 217 627 348 1874 42 1455 1842
Fragments arranged by size:
1874 1842 1455 627 348 325 217 42
BanII G_rGCy'C
Cuts at: 1391 2712 4049 1391
Size: 1321 1337 4072
Fragments arranged by size:
4072 1337 1321
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2385 3725 3758 3890 928
Size: 971 486 1340 33 132 3768
Fragments arranged by size:
3768 1340 971 486 132 33
BbvCI CC'TCA_GC
Cuts at: 4025 4025
Size: 6730
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2666 2700 4921 4955 1903
Size: 34 729 34 2221 34 3678
Fragments arranged by size:
3678 2221 729 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4622 5832 4622
Size: 1210 5520
BclI T'GATC_A
Cuts at: 1548 3147 3702 1548
Size: 1599 555 4576
Fragments arranged by size:
4576 1599 555
BfrBI ATG'CAT
Cuts at: 2489 2977 2489
Size: 488 6242
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5341 136
Size: 107 122 71 631 605 3669 1525
Fragments arranged by size:
3669 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2566 3390 6725 2566
Size: 824 3335 2571
Fragments arranged by size:
3335 2571 824
BlpI GC'TnA_GC
Cuts at: 2688 4147 2688
Size: 1459 5271
Bme1580I G_kGCm'C
Cuts at: 1790 2138 2167 4791 5947 1790
Size: 348 29 2624 1156 2573
Fragments arranged by size:
2624 2573 1156 348 29
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6730
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3000 4461 5419
Size: 416 53 83 399 24 1583 1461 958
Cuts at: 5419 442
Size: 1753
Fragments arranged by size:
1753 1583 1461 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2145 5390 1239
Size: 906 3245 2579
Fragments arranged by size:
3245 2579 906
Bpu10I CC'TnA_GC
Cuts at: 3971 4025 3971
Size: 54 6676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2559 4855 5720 6018 2559
Size: 2296 865 298 3271
Fragments arranged by size:
3271 2296 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3436 3555 5393 882
Size: 617 1937 119 1838 2219
Fragments arranged by size:
2219 1937 1838 617 119
BsaAI yAC'GTr
Cuts at: 493 1204 4521 493
Size: 711 3317 2702
Fragments arranged by size:
3317 2702 711
BsaBI GATnn'nnATC
Cuts at: 6724 6724
Size: 6730
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4537 4919 5554 275
Size: 53 83 186 936 3004 382 635 1451
Fragments arranged by size:
3004 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2294 3759 5163 5561 5835 5982 6393 2294
Size: 1465 1404 398 274 147 411 2631
Fragments arranged by size:
2631 1465 1404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2748 2778 2889 2919 1178
Size: 30 165 30 1345 30 111 30 4989
Fragments arranged by size:
4989 1345 165 111 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2769 3401 1186
Size: 1583 632 4515
Fragments arranged by size:
4515 1583 632
BseYI C'CCAG_C
Cuts at: 5933 6429 5933
Size: 496 6234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6157
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4180 4941 5969 1540
Size: 275 186 2179 761 1028 2301
Fragments arranged by size:
2301 2179 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2167 4791 4876 5947 2167
Size: 2624 85 1071 2950
Fragments arranged by size:
2950 2624 1071 85
BsmI GAATG_Cn'
Cuts at: 2491 3608 4241 4334 2491
Size: 1117 633 93 4887
Fragments arranged by size:
4887 1117 633 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 2167 2712 4049 4791 4876 5947
Size: 399 348 29 545 1337 742 85 1071
Cuts at: 5947 1391
Size: 2174
Fragments arranged by size:
2174 1337 1071 742 545 399 348 85
29
BspEI T'CCGG_A
Cuts at: 3759 6393 3759
Size: 2634 4096
BspHI T'CATG_A
Cuts at: 3501 4619 6349 3501
Size: 1118 1730 3882
Fragments arranged by size:
3882 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4085 844
Size: 3241 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4617 1394
Size: 429 2794 3507
Fragments arranged by size:
3507 2794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3792 5225 5399 65
Size: 1183 2544 1433 174 1396
Fragments arranged by size:
2544 1433 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3575 5374 5561 1700
Size: 110 1765 1799 187 2869
Fragments arranged by size:
2869 1799 1765 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6730
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6730
BssSI C'ACGA_G
Cuts at: 2790 4790 5802 2790
Size: 2000 1012 3718
Fragments arranged by size:
3718 2000 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2698 794
Size: 1904 4826
BstBI TT'CG_AA
Cuts at: 5534 5534
Size: 6730
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6730
BstXI CCAn_nnnn'nTGG
Cuts at: 2024 3171 4020 2024
Size: 1147 849 4734
Fragments arranged by size:
4734 1147 849
BstYI r'GATC_y
Cuts at: 1972 2566 3160 3390 4060 4427 4812 4829 6281
Size: 594 594 230 670 367 385 17 1452
Cuts at: 6281 6367 6379 6725 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 670 594 594 385 367 346
230 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5991
Fragments arranged by size:
5991 683 56
BtsI GCAGTG_nn'
Cuts at: 2447 4246 5040 5060 2447
Size: 1799 794 20 4117
Fragments arranged by size:
4117 1799 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6730
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6695
DraI TTT'AAA
Cuts at: 4034 4034
Size: 6730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5737 809
Size: 507 4421 1802
Fragments arranged by size:
4421 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2743 3475 3831
Size: 54 1425 50 275 18 913 732 356
Cuts at: 3831 3910 4177 5066 6695 8
Size: 79 267 889 1629 43
Fragments arranged by size:
1629 1425 913 889 732 356 275 267
79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4177 1537
Size: 275 2365 4090
Fragments arranged by size:
4090 2365 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3329 3434 3855 4660 1353
Size: 1976 105 421 805 3423
Fragments arranged by size:
3423 1976 805 421 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5317 5691 5837 387
Size: 1064 138 3728 374 146 1280
Fragments arranged by size:
3728 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2145 3879 4793 5390 6177 6445 1239
Size: 393 513 1734 914 597 787 268 1524
Fragments arranged by size:
1734 1524 914 787 597 513 393 268
EcoRI G'AATT_C
Cuts at: 3763 3937 4039 4417 3763
Size: 174 102 378 6076
Fragments arranged by size:
6076 378 174 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3538 3833 3538
Size: 295 6435
FspI TGC'GCA
Cuts at: 5236 5236
Size: 6730
HaeII r_GCGC'y
Cuts at: 1678 5877 1678
Size: 4199 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2951 2983 2992
Size: 32 135 32 488 32 859 32 9
Cuts at: 2992 3024 3395 3427 3435 3467 5371 5403 5445
Size: 32 371 32 8 32 1904 32 42
Cuts at: 5445 5477 1373
Size: 32 2626
Fragments arranged by size:
2626 1904 859 488 371 135 42 32
32 32 32 32 32 32 32 32
9 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2116 3011 4074 4320 669
Size: 988 281 178 895 1063 246 3079
Fragments arranged by size:
3079 1063 988 895 281 246 178
HindIII A'AGCT_T
Cuts at: 748 3235 748
Size: 2487 4243
HpaI GTT'AAC
Cuts at: 4320 4320
Size: 6730
KpnI G_GTAC'C
Cuts at: 4013 4055 4013
Size: 42 6688
MfeI C'AATT_G
Cuts at: 3930 4329 3930
Size: 399 6331
MluI A'CGCG_T
Cuts at: 6606 6606
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2151 2431 2580 5844 6028 1305
Size: 846 280 149 3264 184 2007
Fragments arranged by size:
3264 2007 846 280 184 149
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2745 3477 3833 3912 10
Size: 54 1425 343 913 732 356 79 2828
Fragments arranged by size:
2828 1425 913 732 356 343 79 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2022 2141 4688 5047 5206 518
Size: 648 856 119 2547 359 159 2042
Fragments arranged by size:
2547 2042 856 648 359 159 119
MspA1I CmG'CkG
Cuts at: 1606 3629 4146 4823 5971 6216 1606
Size: 2023 517 677 1148 245 2120
Fragments arranged by size:
2120 2023 1148 677 517 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6178
NdeI CA'TA_TG
Cuts at: 387 2019 387
Size: 1632 5098
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6730
NotI GC'GGCC_GC
Cuts at: 4177 4177
Size: 6730
NsiI A_TGCA'T
Cuts at: 2491 2979 2491
Size: 488 6242
NspI r_CATG'y
Cuts at: 1132 1229 2033 4088 5633 1132
Size: 97 804 2055 1545 2229
Fragments arranged by size:
2229 2055 1545 804 97
PacI TTA_AT'TAA
Cuts at: 3981 3981
Size: 6730
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6730
PciI A'CATG_T
Cuts at: 5629 5629
Size: 6730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6730
PmeI GTTT'AAAC
Cuts at: 4034 4034
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2889 2921 4799 4831 6312 6344 2889
Size: 32 1878 32 1481 32 3275
Fragments arranged by size:
3275 1878 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2993 1613
Size: 1380 5350
PsiI TTA'TAA
Cuts at: 4300 4300
Size: 6730
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2757 2789 2757
Size: 32 6698
PstI C_TGCA'G
Cuts at: 830 2872 3535 4082 830
Size: 2042 663 547 3478
Fragments arranged by size:
3478 2042 663 547
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6730
PvuII CAG'CTG
Cuts at: 3629 3629
Size: 6730
SalI G'TCGA_C
Cuts at: 1936 4072 1936
Size: 2136 4594
SbfI CC_TGCA'GG
Cuts at: 4082 4082
Size: 6730
ScaI AGT'ACT
Cuts at: 4978 6690 4978
Size: 1712 5018
SfcI C'TryA_G
Cuts at: 826 1045 2811 2868 3181 3531 4078 5213 5894
Size: 219 1766 57 313 350 547 1135 681
Cuts at: 5894 6085 826
Size: 191 1471
Fragments arranged by size:
1766 1471 1135 681 547 350 313 219
191 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6730
SmaI CCC'GGG
Cuts at: 1872 4057 1872
Size: 2185 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2264 2574 4834 5735 5997 820
Size: 197 926 321 310 2260 901 262 1553
Fragments arranged by size:
2260 1553 926 901 321 310 262 197
SnaBI TAC'GTA
Cuts at: 493 4521 493
Size: 4028 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6730
SphI G_CATG'C
Cuts at: 1132 4088 1132
Size: 2956 3774
SspI AAT'ATT
Cuts at: 5 52 2367 4654 5
Size: 47 2315 2287 2081
Fragments arranged by size:
2315 2287 2081 47
StuI AGG'CCT
Cuts at: 1517 3179 1517
Size: 1662 5068
StyI C'CwwG_G
Cuts at: 513 1065 3033 4169 513
Size: 552 1968 1136 3074
Fragments arranged by size:
3074 1968 1136 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2795 4757 4774 4927 5112 5557 325
Size: 1255 1215 1962 17 153 185 445 1498
Fragments arranged by size:
1962 1498 1255 1215 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2515 4976 6688
Size: 274 80 33 51 1443 538 2461 1712
Cuts at: 6688 96
Size: 138
Fragments arranged by size:
2461 1712 1443 538 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6690 6722 6690
Size: 32 6698
Tth111I GACn'n_nGTC
Cuts at: 1316 2834 1316
Size: 1518 5212
XbaI T'CTAG_A
Cuts at: 2908 4066 2908
Size: 1158 5572
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6730
XmaI C'CCGG_G
Cuts at: 1870 4055 1870
Size: 2185 4545
XmnI GAAnn'nnTTC
Cuts at: 3816 4859 3816
Size: 1043 5687
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4538 276
Size: 53 83 186 936 3004 2468
Fragments arranged by size:
3004 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PacI PasI PciI PflMI PmeI PpiI PshAI
PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI