Restriction map for
pFN21AB9499
(Circular) (Six-base) MAPSORT of: pFN21AB9499.seq Check: 388 from: 1 to: 8107
pFN21AB9499 8107 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4117 4117
Size: 8107
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4373 5917 278
Size: 53 83 186 936 2837 1544 2468
Fragments arranged by size:
2837 2468 1544 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3812 5015 5450 1937
Size: 1875 1203 435 4594
Fragments arranged by size:
4594 1875 1203 435
Acc65I G'GTAC_C
Cuts at: 5428 5428
Size: 8107
AclI AA'CG_TT
Cuts at: 1563 6234 6607 1563
Size: 4671 373 3063
Fragments arranged by size:
4671 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2550 3347 5161 6170 7554 7822 1632
Size: 590 328 797 1814 1009 1384 268 1917
Fragments arranged by size:
1917 1814 1384 1009 797 590 328 268
AfeI AGC'GCT
Cuts at: 5182 5182
Size: 8107
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7910
AflIII A'CryG_T
Cuts at: 1203 7006 7983 1203
Size: 5803 977 1327
Fragments arranged by size:
5803 1327 977
AgeI A'CCGG_T
Cuts at: 6938 6938
Size: 8107
AhdI GACnn_n'nnGTC
Cuts at: 6836 6836
Size: 8107
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8107
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3386 1184
Size: 2202 5905
AlwNI CAG_nnn'CTG
Cuts at: 2135 2528 2839 3610 4234 4397 5234 5353 5850
Size: 393 311 771 624 163 837 119 497
Cuts at: 5850 7422 2135
Size: 1572 2820
Fragments arranged by size:
2820 1572 837 771 624 497 393 311
163 119
ApaI G_GGCC'C
Cuts at: 4383 4493 4383
Size: 110 7997
ApaLI G'TGCA_C
Cuts at: 6164 7320 6164
Size: 1156 6951
ApoI r'AATT_y
Cuts at: 1493 2324 2756 3041 4079 5416 5642 5794 7903
Size: 831 432 285 1038 1337 226 152 2109
Cuts at: 7903 1493
Size: 1697
Fragments arranged by size:
2109 1697 1337 1038 831 432 285 226
152
AseI AT'TA_AT
Cuts at: 160 1032 3168 6661 160
Size: 872 2136 3493 1606
Fragments arranged by size:
3493 2136 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
AvaI C'yCGr_G
Cuts at: 1870 1943 2494 3201 3913 4762 5240 5432 1870
Size: 73 551 707 712 849 478 192 4545
Fragments arranged by size:
4545 849 712 707 551 478 192 73
BamHI G'GATC_C
Cuts at: 5437 5804 5437
Size: 367 7740
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2855 3744 5329 5428 6883
Size: 325 217 627 1068 889 1585 99 1455
Cuts at: 6883 618
Size: 1842
Fragments arranged by size:
1842 1585 1455 1068 889 627 325 217
99
BanII G_rGCy'C
Cuts at: 1391 2016 3927 4383 4493 5123 5426 1391
Size: 625 1911 456 110 630 303 4072
Fragments arranged by size:
4072 1911 630 625 456 303 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3999 4547 5264 928
Size: 971 2100 548 717 3771
Fragments arranged by size:
3771 2100 971 717 548
BbvCI CC'TCA_GC
Cuts at: 3129 3129
Size: 8107
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6298 6332 1903
Size: 34 4361 34 3678
Fragments arranged by size:
4361 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5999 7209 5999
Size: 1210 6897
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8107
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3685 6718 136
Size: 107 122 71 631 605 2013 3033 1525
Fragments arranged by size:
3033 2013 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2260 8102 2260
Size: 5842 2265
BlpI GC'TnA_GC
Cuts at: 3209 4347 5524 3209
Size: 1138 1177 5792
Fragments arranged by size:
5792 1177 1138
Bme1580I G_kGCm'C
Cuts at: 1790 3747 4383 4493 5334 6168 7324 1790
Size: 1957 636 110 841 834 1156 2573
Fragments arranged by size:
2573 1957 1156 841 834 636 110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4250 5838 6796
Size: 416 53 83 399 24 2833 1588 958
Cuts at: 6796 442
Size: 1753
Fragments arranged by size:
2833 1753 1588 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4194 1056
Size: 3138 4969
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 8107
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2265 2870 2894 2958 3107 3323 3617 3632
Size: 1026 605 24 64 149 216 294 15
Cuts at: 3632 3995 4469 4591 4896 5275 6767 1239
Size: 363 474 122 305 379 1492 2579
Fragments arranged by size:
2579 1492 1026 605 474 379 363 305
294 216 149 122 64 24 15
Bpu10I CC'TnA_GC
Cuts at: 2832 3111 3129 4652 2832
Size: 279 18 1523 6287
Fragments arranged by size:
6287 1523 279 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3266 3332 6232 7097 7395 3266
Size: 66 2900 865 298 3978
Fragments arranged by size:
3978 2900 865 298 66
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5212 6770 882
Size: 617 3713 1558 2219
Fragments arranged by size:
3713 2219 1558 617
BsaAI yAC'GTr
Cuts at: 493 1204 5898 493
Size: 711 4694 2702
Fragments arranged by size:
4694 2702 711
BsaBI GATnn'nnATC
Cuts at: 8101 8101
Size: 8107
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4370 5914 6296 6931
Size: 53 83 186 936 2837 1544 382 635
Cuts at: 6931 275
Size: 1451
Fragments arranged by size:
2837 1544 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3471 6540 6938 7212 7359 7770 3471
Size: 3069 398 274 147 411 3808
Fragments arranged by size:
3808 3069 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3557 3587 1178
Size: 30 165 30 2154 30 5698
Fragments arranged by size:
5698 2154 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2807 2810 3461 3951 4006 5053 5179
Size: 913 708 3 651 490 55 1047 126
Cuts at: 5179 1186
Size: 4114
Fragments arranged by size:
4114 1047 913 708 651 490 126 55
3
BseYI C'CCAG_C
Cuts at: 2090 4067 4630 7310 7806 2090
Size: 1977 563 2680 496 2391
Fragments arranged by size:
2680 2391 1977 563 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4077 4560 1163
Size: 573 2341 483 4710
Fragments arranged by size:
4710 2341 573 483
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5557 6318 7346 1540
Size: 275 186 3556 761 1028 2301
Fragments arranged by size:
3556 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3292 3927 5123 5172 6168 6253 7324 3292
Size: 635 1196 49 996 85 1071 4075
Fragments arranged by size:
4075 1196 1071 996 635 85 49
BsmI GAATG_Cn'
Cuts at: 3407 5618 5711 3407
Size: 2211 93 5803
Fragments arranged by size:
5803 2211 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 8107
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2016 3292 3747 3927 4383 4493 5123
Size: 399 226 1276 455 180 456 110 630
Cuts at: 5123 5172 5334 5426 6168 6253 7324 1391
Size: 49 162 92 742 85 1071 2174
Fragments arranged by size:
2174 1276 1071 742 630 456 455 399
226 180 162 110 92 85 49
BspEI T'CCGG_A
Cuts at: 7770 7770
Size: 8107
BspHI T'CATG_A
Cuts at: 5996 7726 5996
Size: 1730 6377
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4117 5462 844
Size: 3273 1345 3489
Fragments arranged by size:
3489 3273 1345
BsrBI CCG'CTC
Cuts at: 1394 1823 3225 5994 1394
Size: 429 1402 2769 3507
Fragments arranged by size:
3507 2769 1402 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3864 6602 6776 65
Size: 1183 2616 2738 174 1396
Fragments arranged by size:
2738 2616 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2122 2672 4430 5057 6751 6938 1700
Size: 110 312 550 1758 627 1694 187 2869
Fragments arranged by size:
2869 1758 1694 627 550 312 187 110
BsrGI T'GTAC_A
Cuts at: 96 4136 96
Size: 4040 4067
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8107
BssSI C'ACGA_G
Cuts at: 6167 7179 6167
Size: 1012 7095
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3428 5353 794
Size: 2634 1925 3548
Fragments arranged by size:
3548 2634 1925
BstBI TT'CG_AA
Cuts at: 6911 6911
Size: 8107
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8107
BstXI CCAn_nnnn'nTGG
Cuts at: 2926 3439 4042 5261 2926
Size: 513 603 1219 5772
Fragments arranged by size:
5772 1219 603 513
BstYI r'GATC_y
Cuts at: 1972 2260 2269 5437 5804 6189 6206 7658 7744
Size: 288 9 3168 367 385 17 1452 86
Cuts at: 7744 7756 8102 1972
Size: 12 346 1977
Fragments arranged by size:
3168 1977 1452 385 367 346 288 86
17 12 9
Bsu36I CC'TnA_GG
Cuts at: 4588 4588
Size: 8107
BtgI C'CryG_G
Cuts at: 513 1065 2925 3739 4364 4827 5254 5396 513
Size: 552 1860 814 625 463 427 142 3224
Fragments arranged by size:
3224 1860 814 625 552 463 427 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3383 487
Size: 683 56 2157 5211
Fragments arranged by size:
5211 2157 683 56
BtsI GCAGTG_nn'
Cuts at: 2919 4085 5623 6417 6437 2919
Size: 1166 1538 794 20 4589
Fragments arranged by size:
4589 1538 1166 794 20
ClaI AT'CG_AT
Cuts at: 1554 2382 2421 1554
Size: 828 39 7240
Fragments arranged by size:
7240 828 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5385 5420 529
Size: 35 4821 35 3216
Fragments arranged by size:
4821 3216 35 35
DraI TTT'AAA
Cuts at: 5411 5411
Size: 8107
DraIII CAC_nnn'GTG
Cuts at: 4512 4512
Size: 8107
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 7114 809
Size: 507 3659 2139 1802
Fragments arranged by size:
3659 2139 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2916 3384 3762
Size: 54 1425 50 275 18 1086 468 378
Cuts at: 3762 4548 5055 5263 5554 6443 8072 8
Size: 786 507 208 291 889 1629 43
Fragments arranged by size:
1629 1425 1086 889 786 507 468 378
291 275 208 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5554 1537
Size: 275 3742 4090
Fragments arranged by size:
4090 3742 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3061 3438 4648 4762 6037 1353
Size: 1708 377 1210 114 1275 3423
Fragments arranged by size:
3423 1708 1275 1210 377 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6694 7068 7214 387
Size: 1064 138 5105 374 146 1280
Fragments arranged by size:
5105 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3925 5121 3925
Size: 1196 6911
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2265 2550 2870 2894 2958 3107
Size: 393 590 43 285 320 24 64 149
Cuts at: 3107 3323 3347 3617 3632 3995 4469 4591 4896
Size: 216 24 270 15 363 474 122 305
Cuts at: 4896 5161 5275 6170 6767 7554 7822 1239
Size: 265 114 895 597 787 268 1524
Fragments arranged by size:
1524 895 787 597 590 474 393 363
320 305 285 270 268 265 216 149
122 114 64 43 24 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3916 4313 3916
Size: 397 7710
EcoO109I rG'GnC_Cy
Cuts at: 2311 2836 3527 3607 3909 4318 4380 4489 4944
Size: 525 691 80 302 409 62 109 455
Cuts at: 4944 5231 5244 5384 2311
Size: 287 13 140 5034
Fragments arranged by size:
5034 691 525 455 409 302 287 140
109 80 62 13
EcoRI G'AATT_C
Cuts at: 3041 4079 5416 5794 3041
Size: 1038 1337 378 5354
Fragments arranged by size:
5354 1337 1038 378
FspI TGC'GCA
Cuts at: 6613 6613
Size: 8107
HaeII r_GCGC'y
Cuts at: 1678 5184 5312 7254 1678
Size: 3506 128 1942 2531
Fragments arranged by size:
3506 2531 1942 128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2630 2662 3914 3946 3991
Size: 32 135 32 1058 32 1252 32 45
Cuts at: 3991 4023 6748 6780 6822 6854 1373
Size: 32 2725 32 42 32 2626
Fragments arranged by size:
2725 2626 1252 1058 135 45 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3970 4896 5451 5697 669
Size: 988 281 2032 926 555 246 3079
Fragments arranged by size:
3079 2032 988 926 555 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8107
HpaI GTT'AAC
Cuts at: 5697 5697
Size: 8107
KpnI G_GTAC'C
Cuts at: 5432 5432
Size: 8107
MfeI C'AATT_G
Cuts at: 5706 5706
Size: 8107
MluI A'CGCG_T
Cuts at: 7983 7983
Size: 8107
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2433 4102 7221 7405 1305
Size: 684 444 1669 3119 184 2007
Fragments arranged by size:
3119 2007 1669 684 444 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3386 3764 5265 10
Size: 54 1425 343 1554 378 1501 2852
Fragments arranged by size:
2852 1554 1501 1425 378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2924 3306 6065 6424 6583 518
Size: 648 1758 382 2759 359 159 2042
Fragments arranged by size:
2759 2042 1758 648 382 359 159
MspA1I CmG'CkG
Cuts at: 1606 2135 2825 4067 4547 5523 6200 7348 7593
Size: 529 690 1242 480 976 677 1148 245
Cuts at: 7593 1606
Size: 2120
Fragments arranged by size:
2120 1242 1148 976 690 677 529 480
245
NaeI GCC'GGC
Cuts at: 2124 2674 4432 5059 2124
Size: 550 1758 627 5172
Fragments arranged by size:
5172 1758 627 550
NcoI C'CATG_G
Cuts at: 513 1065 2925 3739 5254 513
Size: 552 1860 814 1515 3366
Fragments arranged by size:
3366 1860 1515 814 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8107
NgoMIV G'CCGG_C
Cuts at: 2122 2672 4430 5057 2122
Size: 550 1758 627 5172
Fragments arranged by size:
5172 1758 627 550
NheI G'CTAG_C
Cuts at: 1052 4190 1052
Size: 3138 4969
NotI GC'GGCC_GC
Cuts at: 5554 5554
Size: 8107
NspI r_CATG'y
Cuts at: 1132 1229 5465 7010 1132
Size: 97 4236 1545 2229
Fragments arranged by size:
4236 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 4948 1348
Size: 3600 4507
PciI A'CATG_T
Cuts at: 7006 7006
Size: 8107
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3610 3696 4954 1108
Size: 2502 86 1258 4261
Fragments arranged by size:
4261 2502 1258 86
PfoI T'CCnGG_A
Cuts at: 2075 2075
Size: 8107
PmeI GTTT'AAAC
Cuts at: 5411 5411
Size: 8107
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6176 6208 7689 7721 6176
Size: 32 1481 32 6562
Fragments arranged by size:
6562 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3527 3607 4944 5244 3527
Size: 80 1337 300 6390
Fragments arranged by size:
6390 1337 300 80
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8107
PsiI TTA'TAA
Cuts at: 5141 5677 5141
Size: 536 7571
PspOMI G'GGCC_C
Cuts at: 4379 4489 4379
Size: 110 7997
PspXI vC'TCGA_Gb
Cuts at: 3913 3913
Size: 8107
PstI C_TGCA'G
Cuts at: 830 2131 2508 3266 4114 4203 4533 4690 4996
Size: 1301 377 758 848 89 330 157 306
Cuts at: 4996 5459 830
Size: 463 3478
Fragments arranged by size:
3478 1301 848 758 463 377 330 306
157 89
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8107
PvuII CAG'CTG
Cuts at: 2135 4067 2135
Size: 1932 6175
SacI G_AGCT'C
Cuts at: 3927 5123 3927
Size: 1196 6911
SalI G'TCGA_C
Cuts at: 1936 5449 1936
Size: 3513 4594
SanDI GG'GwC_CC
Cuts at: 4944 4944
Size: 8107
SapI GCTCTTCn'nnn_
Cuts at: 4762 4762
Size: 8107
SbfI CC_TGCA'GG
Cuts at: 5459 5459
Size: 8107
ScaI AGT'ACT
Cuts at: 6355 8067 6355
Size: 1712 6395
SfcI C'TryA_G
Cuts at: 826 1045 2127 2504 3262 4110 4199 4529 4686
Size: 219 1082 377 758 848 89 330 157
Cuts at: 4686 4992 5455 6590 7271 7462 826
Size: 306 463 1135 681 191 1471
Fragments arranged by size:
1471 1135 1082 848 758 681 463 377
330 306 219 191 157 89
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8107
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8107
SmaI CCC'GGG
Cuts at: 1872 5242 5434 1872
Size: 3370 192 4545
Fragments arranged by size:
4545 3370 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3281 3347 3913 6211 7112 7374
Size: 197 926 1338 66 566 2298 901 262
Cuts at: 7374 820
Size: 1553
Fragments arranged by size:
2298 1553 1338 926 901 566 262 197
66
SnaBI TAC'GTA
Cuts at: 493 5898 493
Size: 5405 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8107
SphI G_CATG'C
Cuts at: 1132 5465 1132
Size: 4333 3774
SspI AAT'ATT
Cuts at: 5 52 2720 6031 5
Size: 47 2668 3311 2081
Fragments arranged by size:
3311 2668 2081 47
StuI AGG'CCT
Cuts at: 1517 2235 3072 3897 4596 1517
Size: 718 837 825 699 5028
Fragments arranged by size:
5028 837 825 718 699
StyI C'CwwG_G
Cuts at: 513 1065 2925 3019 3739 4035 5130 5211 5254
Size: 552 1860 94 720 296 1095 81 43
Cuts at: 5254 5260 5546 513
Size: 6 286 3074
Fragments arranged by size:
3074 1860 1095 720 552 296 286 94
81 43 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2379 3173 5351 6134 6151 6304 6489
Size: 1255 799 794 2178 783 17 153 185
Cuts at: 6489 6934 325
Size: 445 1498
Fragments arranged by size:
2178 1498 1255 799 794 783 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4136 4330 6353
Size: 274 80 33 51 1443 2159 194 2023
Cuts at: 6353 8065 96
Size: 1712 138
Fragments arranged by size:
2159 2023 1712 1443 274 194 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3557 3589 8067 8099 3557
Size: 32 4478 32 3565
Fragments arranged by size:
4478 3565 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5011 1316
Size: 3695 4412
XbaI T'CTAG_A
Cuts at: 5443 5443
Size: 8107
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3167 5258 3167
Size: 2091 6016
XhoI C'TCGA_G
Cuts at: 1943 3913 1943
Size: 1970 6137
XmaI C'CCGG_G
Cuts at: 1870 5240 5432 1870
Size: 3370 192 4545
Fragments arranged by size:
4545 3370 192
XmnI GAAnn'nnTTC
Cuts at: 3013 3152 3539 4451 6236 3013
Size: 139 387 912 1785 4884
Fragments arranged by size:
4884 1785 912 387 139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4371 5915 276
Size: 53 83 186 936 2837 1544 2468
Fragments arranged by size:
2837 2468 1544 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI NsiI
PacI PmlI PsrI RsrII SacII SexAI SfiI SfoI
SrfI SwaI