Restriction map for
pFN21AB9497
(Circular) (Six-base) MAPSORT of: pFN21AB9497.seq Check: 1468 from: 1 to: 8230
pFN21AB9497 8230 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3409 3668 3409
Size: 259 7971
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2580 6040 278
Size: 53 83 186 936 1044 3460 2468
Fragments arranged by size:
3460 2468 1044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3728 5573 1937
Size: 1791 1845 4594
Fragments arranged by size:
4594 1845 1791
Acc65I G'GTAC_C
Cuts at: 2413 5551 2413
Size: 3138 5092
AclI AA'CG_TT
Cuts at: 1563 6357 6730 1563
Size: 4794 373 3063
Fragments arranged by size:
4794 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2710 3068 3602 4193 4580 4667 6293 7677
Size: 1078 358 534 591 387 87 1626 1384
Cuts at: 7677 7945 1632
Size: 268 1917
Fragments arranged by size:
1917 1626 1384 1078 591 534 387 358
268 87
AfeI AGC'GCT
Cuts at: 2504 4941 5462 2504
Size: 2437 521 5272
Fragments arranged by size:
5272 2437 521
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8033
AflIII A'CryG_T
Cuts at: 1203 7129 8106 1203
Size: 5926 977 1327
Fragments arranged by size:
5926 1327 977
AgeI A'CCGG_T
Cuts at: 2045 2659 7061 2045
Size: 614 4402 3214
Fragments arranged by size:
4402 3214 614
AhdI GACnn_n'nnGTC
Cuts at: 3158 6959 3158
Size: 3801 4429
AleI CACnn'nnGTG
Cuts at: 1166 3692 4385 4907 5222 1166
Size: 2526 693 522 315 4174
Fragments arranged by size:
4174 2526 693 522 315
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3059 3994 4088 5021 1184
Size: 1875 935 94 933 4393
Fragments arranged by size:
4393 1875 935 933 94
AlwNI CAG_nnn'CTG
Cuts at: 2683 4018 4037 4108 4204 4879 5973 7545 2683
Size: 1335 19 71 96 675 1094 1572 3368
Fragments arranged by size:
3368 1572 1335 1094 675 96 71 19
ApaI G_GGCC'C
Cuts at: 3078 3639 4473 4645 3078
Size: 561 834 172 6663
Fragments arranged by size:
6663 834 561 172
ApaLI G'TGCA_C
Cuts at: 3427 6287 7443 3427
Size: 2860 1156 4214
Fragments arranged by size:
4214 2860 1156
ApoI r'AATT_y
Cuts at: 1493 5539 5765 5917 8026 1493
Size: 4046 226 152 2109 1697
Fragments arranged by size:
4046 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6784 160
Size: 872 5752 1606
Fragments arranged by size:
5752 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8230
AvaI C'yCGr_G
Cuts at: 1870 1943 3138 3935 3986 4272 4355 4711 5555
Size: 73 1195 797 51 286 83 356 844
Cuts at: 5555 1870
Size: 4545
Fragments arranged by size:
4545 1195 844 797 356 286 83 73
51
BamHI G'GATC_C
Cuts at: 2181 4402 5560 5927 2181
Size: 2221 1158 367 4484
Fragments arranged by size:
4484 2221 1158 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2413 2423 3305 3343 3394
Size: 325 217 627 626 10 882 38 51
Cuts at: 3394 3536 3574 3847 4452 4625 5194 5551 7006
Size: 142 38 273 605 173 569 357 1455
Cuts at: 7006 618
Size: 1842
Fragments arranged by size:
1842 1455 882 627 626 605 569 357
325 273 217 173 142 51 38 38
10
BanII G_rGCy'C
Cuts at: 1391 3020 3078 3218 3373 3639 3819 3843 3978
Size: 1629 58 140 155 266 180 24 135
Cuts at: 3978 4473 4645 5258 5549 1391
Size: 495 172 613 291 4072
Fragments arranged by size:
4072 1629 613 495 291 266 180 172
155 140 135 58 24
BbeI G_GCGC'C
Cuts at: 4629 5198 4629
Size: 569 7661
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3056 4571 4780 5131 928
Size: 971 1157 1515 209 351 4027
Fragments arranged by size:
4027 1515 1157 971 351 209
BbvCI CC'TCA_GC
Cuts at: 2087 2600 3671 4463 5312 2087
Size: 513 1071 792 849 5005
Fragments arranged by size:
5005 1071 849 792 513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2008 2042 2455 2489 6421 6455 1903
Size: 34 71 34 413 34 3932 34 3678
Fragments arranged by size:
3932 3678 413 71 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2428 6122 7332 2428
Size: 3694 1210 3326
Fragments arranged by size:
3694 3326 1210
BclI T'GATC_A
Cuts at: 1548 2649 4308 1548
Size: 1101 1659 5470
Fragments arranged by size:
5470 1659 1101
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2990 3067 3634
Size: 107 122 71 631 605 1318 77 567
Cuts at: 3634 4550 4649 6841 136
Size: 916 99 2192 1525
Fragments arranged by size:
2192 1525 1318 916 631 605 567 122
107 99 77 71
BglII A'GATC_T
Cuts at: 4132 8225 4132
Size: 4093 4137
BlpI GC'TnA_GC
Cuts at: 2114 4979 4994 5647 2114
Size: 2865 15 653 4697
Fragments arranged by size:
4697 2865 653 15
Bme1580I G_kGCm'C
Cuts at: 1790 2931 3078 3431 3539 3579 3639 3999 4086
Size: 1141 147 353 108 40 60 360 87
Cuts at: 4086 4473 4535 4645 4740 4989 5229 6291 7447
Size: 387 62 110 95 249 240 1062 1156
Cuts at: 7447 1790
Size: 2573
Fragments arranged by size:
2573 1156 1141 1062 387 360 353 249
240 147 110 108 95 87 62 60
40
BmgBI CAC'GTC
Cuts at: 1318 2158 3160 3562 1318
Size: 840 1002 402 5986
Fragments arranged by size:
5986 1002 840 402
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4813 5961 6919
Size: 416 53 83 399 24 3396 1148 958
Cuts at: 6919 442
Size: 1753
Fragments arranged by size:
3396 1753 1148 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8230
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2375 2591 2899 3665 3764 4112 5411 6890
Size: 1136 216 308 766 99 348 1299 1479
Cuts at: 6890 1239
Size: 2579
Fragments arranged by size:
2579 1479 1299 1136 766 348 308 216
99
Bpu10I CC'TnA_GC
Cuts at: 2087 2600 3671 4332 4463 4557 5252 5312 2087
Size: 513 1071 661 131 94 695 60 5005
Fragments arranged by size:
5005 1071 695 661 513 131 94 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6355 7220 7518 6355
Size: 865 298 7067
Fragments arranged by size:
7067 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2513 3793 4702 6893 882
Size: 617 1014 1280 909 2191 2219
Fragments arranged by size:
2219 2191 1280 1014 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 6021 493
Size: 711 4817 2702
Fragments arranged by size:
4817 2702 711
BsaBI GATnn'nnATC
Cuts at: 3362 8224 3362
Size: 4862 3368
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2577 4626 5195 6037
Size: 53 83 186 936 1044 2049 569 842
Cuts at: 6037 6419 7054 275
Size: 382 635 1451
Fragments arranged by size:
2049 1451 1044 936 842 635 569 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2045 2659 6663 7061 7335 7482 7893 2045
Size: 614 4004 398 274 147 411 2382
Fragments arranged by size:
4004 2382 614 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8005
Fragments arranged by size:
8005 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2083 2474 3290 3851 1186
Size: 897 391 816 561 5565
Fragments arranged by size:
5565 897 816 561 391
BseYI C'CCAG_C
Cuts at: 2177 2442 2523 2676 3070 3735 3819 4085 4101
Size: 265 81 153 394 665 84 266 16
Cuts at: 4101 5064 5350 7433 7929 2177
Size: 963 286 2083 496 2478
Fragments arranged by size:
2478 2083 963 665 496 394 286 266
265 153 84 81 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2345 3105 3175 5054 1163
Size: 573 609 760 70 1879 4339
Fragments arranged by size:
4339 1879 760 609 573 70
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5210 5680 6441 7469 1540
Size: 275 186 3209 470 761 1028 2301
Fragments arranged by size:
3209 2301 1028 761 470 275 186
BsiHKAI G_wGCw'C
Cuts at: 3020 3218 3431 3652 4920 4995 5258 6291 6376
Size: 198 213 221 1268 75 263 1033 85
Cuts at: 6376 7447 3020
Size: 1071 3803
Fragments arranged by size:
3803 1268 1071 1033 263 221 213 198
85 75
BsmI GAATG_Cn'
Cuts at: 3036 3593 5741 5834 3036
Size: 557 2148 93 5432
Fragments arranged by size:
5432 2148 557 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4614 4614
Size: 8230
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2931 3020 3078 3218 3373 3431 3539
Size: 399 1141 89 58 140 155 58 108
Cuts at: 3539 3579 3639 3652 3819 3843 3978 3999 4086
Size: 40 60 13 167 24 135 21 87
Cuts at: 4086 4473 4535 4645 4740 4920 4989 4995 5229
Size: 387 62 110 95 180 69 6 234
Cuts at: 5229 5258 5549 6291 6376 7447 1391
Size: 29 291 742 85 1071 2174
Fragments arranged by size:
2174 1141 1071 742 399 387 291 234
180 167 155 140 135 110 108 95
89 87 85 69 62 60 58 58
40 29 24 21 13 6
BspEI T'CCGG_A
Cuts at: 7893 7893
Size: 8230
BspHI T'CATG_A
Cuts at: 4158 4920 6119 7849 4158
Size: 762 1199 1730 4539
Fragments arranged by size:
4539 1730 1199 762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3409 3668 4257 4878 5121 5585 844
Size: 2565 259 589 621 243 464 3489
Fragments arranged by size:
3489 2565 621 589 464 259 243
BsrBI CCG'CTC
Cuts at: 1394 1823 2565 2586 4336 5212 6117 1394
Size: 429 742 21 1750 876 905 3507
Fragments arranged by size:
3507 1750 905 876 742 429 21
BsrDI GCAATG_nn'
Cuts at: 65 1248 3837 6725 6899 65
Size: 1183 2589 2888 174 1396
Fragments arranged by size:
2888 2589 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2045 2659 2883 2993 3096 3675 3754
Size: 110 235 614 224 110 103 579 79
Cuts at: 3754 5027 5205 5330 5516 6874 7061 1700
Size: 1273 178 125 186 1358 187 2869
Fragments arranged by size:
2869 1358 1273 614 579 235 224 187
186 178 125 110 110 103 79
BsrGI T'GTAC_A
Cuts at: 96 2814 4806 96
Size: 2718 1992 3520
Fragments arranged by size:
3520 2718 1992
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8230
BssSI C'ACGA_G
Cuts at: 2027 6290 7302 2027
Size: 4263 1012 2955
Fragments arranged by size:
4263 2955 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3471 794
Size: 2677 5553
BstBI TT'CG_AA
Cuts at: 7034 7034
Size: 8230
BstEII G'GTnAC_C
Cuts at: 1184 3172 3382 4865 5023 5359 1184
Size: 1988 210 1483 158 336 4055
Fragments arranged by size:
4055 1988 1483 336 210 158
BstXI CCAn_nnnn'nTGG
Cuts at: 2960 4456 4522 4891 4909 2960
Size: 1496 66 369 18 6281
Fragments arranged by size:
6281 1496 369 66 18
BstYI r'GATC_y
Cuts at: 1972 2080 2181 4132 4402 5560 5927 6312 6329
Size: 108 101 1951 270 1158 367 385 17
Cuts at: 6329 7781 7867 7879 8225 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1951 1452 1158 385 367 346 270
108 101 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5525 5525
Size: 8230
BtgI C'CryG_G
Cuts at: 513 1065 2135 2557 2593 3387 3693 3705 4428
Size: 552 1070 422 36 794 306 12 723
Cuts at: 4428 4740 5069 5133 513
Size: 312 329 64 3610
Fragments arranged by size:
3610 1070 794 723 552 422 329 312
306 64 36 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2716 487
Size: 683 56 1490 6001
Fragments arranged by size:
6001 1490 683 56
BtsI GCAGTG_nn'
Cuts at: 3056 3174 3482 3798 4070 4920 5746 6540 6560
Size: 118 308 316 272 850 826 794 20
Cuts at: 6560 3056
Size: 4726
Fragments arranged by size:
4726 850 826 794 316 308 272 118
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8230
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4618 4653 529
Size: 35 4054 35 4106
Fragments arranged by size:
4106 4054 35 35
DraI TTT'AAA
Cuts at: 5534 5534
Size: 8230
DraIII CAC_nnn'GTG
Cuts at: 2161 2161
Size: 8230
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7237 809
Size: 507 5921 1802
Fragments arranged by size:
5921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2386 2560 2885
Size: 54 1425 50 275 18 556 174 325
Cuts at: 2885 2991 3654 3917 4059 4719 4881 4961 5097
Size: 106 663 263 142 660 162 80 136
Cuts at: 5097 5207 5487 5501 5677 6566 8195 8
Size: 110 280 14 176 889 1629 43
Fragments arranged by size:
1629 1425 889 663 660 556 325 280
275 263 176 174 162 142 136 110
106 80 54 50 43 18 14
EagI C'GGCC_G
Cuts at: 1537 1812 5207 5677 1537
Size: 275 3395 470 4090
Fragments arranged by size:
4090 3395 470 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2201 2315 2495 2895 3116 3744 3886 6160
Size: 848 114 180 400 221 628 142 2274
Cuts at: 6160 1353
Size: 3423
Fragments arranged by size:
3423 2274 848 628 400 221 180 142
114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5192 6817 7191 7337 387
Size: 1064 138 3603 1625 374 146 1280
Fragments arranged by size:
3603 1625 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3018 3216 5256 3018
Size: 198 2040 5992
Fragments arranged by size:
5992 2040 198
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2591 2710 2899 3068 3602 3665
Size: 393 743 216 119 189 169 534 63
Cuts at: 3665 3764 4112 4193 4580 4667 5411 6293 6890
Size: 99 348 81 387 87 744 882 597
Cuts at: 6890 7677 7945 1239
Size: 787 268 1524
Fragments arranged by size:
1524 882 787 744 743 597 534 393
387 348 268 216 189 169 119 99
87 81 63
EcoNI CCTnn'n_nnAGG
Cuts at: 2492 3545 4526 5294 2492
Size: 1053 981 768 5428
Fragments arranged by size:
5428 1053 981 768
EcoO109I rG'GnC_Cy
Cuts at: 2055 3074 3075 3120 3205 3635 3636 3739 3777
Size: 1019 1 45 85 430 1 103 38
Cuts at: 3777 4212 4519 4641 4642 5379 2055
Size: 435 307 122 1 737 4906
Fragments arranged by size:
4906 1019 737 435 430 307 122 103
85 45 38 1 1 1
EcoRI G'AATT_C
Cuts at: 5539 5917 5539
Size: 378 7852
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5479 5479
Size: 8230
FspI TGC'GCA
Cuts at: 3488 6736 3488
Size: 3248 4982
HaeII r_GCGC'y
Cuts at: 1678 2506 4629 4762 4943 4976 5198 5249 5312
Size: 828 2123 133 181 33 222 51 63
Cuts at: 5312 5464 7377 1678
Size: 152 1913 2531
Fragments arranged by size:
2531 2123 1913 828 222 181 152 133
63 51 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3144 3176 4430 4462 6871
Size: 32 135 32 1572 32 1254 32 2409
Cuts at: 6871 6903 6945 6977 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2409 1572 1254 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5574 5820 669
Size: 988 281 3636 246 3079
Fragments arranged by size:
3636 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4592 748
Size: 3844 4386
HpaI GTT'AAC
Cuts at: 5820 5820
Size: 8230
KasI G'GCGC_C
Cuts at: 4625 5194 4625
Size: 569 7661
KpnI G_GTAC'C
Cuts at: 2417 5555 2417
Size: 3138 5092
MfeI C'AATT_G
Cuts at: 5829 5829
Size: 8230
MluI A'CGCG_T
Cuts at: 8106 8106
Size: 8230
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2183 7344 7528 1305
Size: 878 5161 184 2007
Fragments arranged by size:
5161 2007 878 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3656 3919 4061 4721 4883
Size: 54 1425 343 1824 263 142 660 162
Cuts at: 4883 10
Size: 3357
Fragments arranged by size:
3357 1824 1425 660 343 263 162 142
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2870 3041 3422 3692 3902 4385 4907
Size: 648 1704 171 381 270 210 483 522
Cuts at: 4907 5222 6188 6547 6706 518
Size: 315 966 359 159 2042
Fragments arranged by size:
2042 1704 966 648 522 483 381 359
315 270 210 171 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2680 3514 3823 4034 4259 4467 5305
Size: 512 562 834 309 211 225 208 838
Cuts at: 5305 5473 5509 5646 6323 7471 7716 1606
Size: 168 36 137 677 1148 245 2120
Fragments arranged by size:
2120 1148 838 834 677 562 512 309
245 225 211 208 168 137 36
NaeI GCC'GGC
Cuts at: 2885 2995 3677 3756 5207 5518 2885
Size: 110 682 79 1451 311 5597
Fragments arranged by size:
5597 1451 682 311 110 79
NarI GG'CG_CC
Cuts at: 4626 5195 4626
Size: 569 7661
NcoI C'CATG_G
Cuts at: 513 1065 2135 2557 2593 3705 4740 5069 513
Size: 552 1070 422 36 1112 1035 329 3674
Fragments arranged by size:
3674 1112 1070 1035 552 422 329 36
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8230
NgoMIV G'CCGG_C
Cuts at: 2883 2993 3675 3754 5205 5516 2883
Size: 110 682 79 1451 311 5597
Fragments arranged by size:
5597 1451 682 311 110 79
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8230
NotI GC'GGCC_GC
Cuts at: 5677 5677
Size: 8230
NspI r_CATG'y
Cuts at: 1132 1229 4615 5588 7133 1132
Size: 97 3386 973 1545 2229
Fragments arranged by size:
3386 2229 1545 973 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8230
PciI A'CATG_T
Cuts at: 7129 7129
Size: 8230
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2683 3787 1108
Size: 1575 1104 5551
Fragments arranged by size:
5551 1575 1104
PfoI T'CCnGG_A
Cuts at: 2550 2607 3681 3741 2550
Size: 57 1074 60 7039
Fragments arranged by size:
7039 1074 60 57
PmeI GTTT'AAAC
Cuts at: 5534 5534
Size: 8230
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6299 6331 7812 7844 6299
Size: 32 1481 32 6685
Fragments arranged by size:
6685 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2055 3120 3739 3777 4212 4519 2055
Size: 1065 619 38 435 307 5766
Fragments arranged by size:
5766 1065 619 435 307 38
PshAI GACnn'nnGTC
Cuts at: 1613 3782 1613
Size: 2169 6061
PsiI TTA'TAA
Cuts at: 5800 5800
Size: 8230
PspOMI G'GGCC_C
Cuts at: 3074 3635 4469 4641 3074
Size: 561 834 172 6663
Fragments arranged by size:
6663 834 561 172
PspXI vC'TCGA_Gb
Cuts at: 4711 4711
Size: 8230
PstI C_TGCA'G
Cuts at: 830 3513 3763 3828 3894 4023 4254 4875 5013
Size: 2683 250 65 66 129 231 621 138
Cuts at: 5013 5179 5298 5582 830
Size: 166 119 284 3478
Fragments arranged by size:
3478 2683 621 284 250 231 166 138
129 119 66 65
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8230
PvuII CAG'CTG
Cuts at: 2680 3514 3823 4034 5473 2680
Size: 834 309 211 1439 5437
Fragments arranged by size:
5437 1439 834 309 211
SacI G_AGCT'C
Cuts at: 3020 3218 5258 3020
Size: 198 2040 5992
Fragments arranged by size:
5992 2040 198
SalI G'TCGA_C
Cuts at: 1936 5572 1936
Size: 3636 4594
SanDI GG'GwC_CC
Cuts at: 2055 3120 3777 2055
Size: 1065 657 6508
Fragments arranged by size:
6508 1065 657
SapI GCTCTTCn'nnn_
Cuts at: 2201 2315 3744 2201
Size: 114 1429 6687
Fragments arranged by size:
6687 1429 114
SbfI CC_TGCA'GG
Cuts at: 5298 5582 5298
Size: 284 7946
ScaI AGT'ACT
Cuts at: 4601 6478 8190 4601
Size: 1877 1712 4641
Fragments arranged by size:
4641 1877 1712
SexAI A'CCwGG_T
Cuts at: 2538 3378 4890 2538
Size: 840 1512 5878
Fragments arranged by size:
5878 1512 840
SfcI C'TryA_G
Cuts at: 826 1045 3313 3509 3759 3824 3890 4019 4250
Size: 219 2268 196 250 65 66 129 231
Cuts at: 4250 4586 4871 5009 5175 5294 5578 6713 7394
Size: 336 285 138 166 119 284 1135 681
Cuts at: 7394 7585 826
Size: 191 1471
Fragments arranged by size:
2268 1471 1135 681 336 285 284 250
231 219 196 191 166 138 129 119
66 65
SfoI GGC'GCC
Cuts at: 4627 5196 4627
Size: 569 7661
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8230
SgrAI Cr'CCGG_yG
Cuts at: 1700 3096 1700
Size: 1396 6834
SmaI CCC'GGG
Cuts at: 1872 3140 4274 5557 1872
Size: 1268 1134 1283 4545
Fragments arranged by size:
4545 1283 1268 1134
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4355 4711 6334 7235 7497 820
Size: 197 926 2412 356 1623 901 262 1553
Fragments arranged by size:
2412 1623 1553 926 901 356 262 197
SnaBI TAC'GTA
Cuts at: 493 6021 493
Size: 5528 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8230
SphI G_CATG'C
Cuts at: 1132 5588 1132
Size: 4456 3774
SrfI GCCC'GGGC
Cuts at: 3140 3140
Size: 8230
SspI AAT'ATT
Cuts at: 5 52 6154 5
Size: 47 6102 2081
Fragments arranged by size:
6102 2081 47
StuI AGG'CCT
Cuts at: 1517 2723 3941 1517
Size: 1206 1218 5806
Fragments arranged by size:
5806 1218 1206
StyI C'CwwG_G
Cuts at: 513 1065 2135 2557 2593 3705 4515 4740 5069
Size: 552 1070 422 36 1112 810 225 329
Cuts at: 5069 5187 5669 513
Size: 118 482 3074
Fragments arranged by size:
3074 1112 1070 810 552 482 422 329
225 118 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2896 3495 4340 4853 6257 6274 6427
Size: 1255 1316 599 845 513 1404 17 153
Cuts at: 6427 6612 7057 325
Size: 185 445 1498
Fragments arranged by size:
1498 1404 1316 1255 845 599 513 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 4599 4794
Size: 274 80 33 51 1443 837 1785 195
Cuts at: 4794 4806 6476 8188 96
Size: 12 1670 1712 138
Fragments arranged by size:
1785 1712 1670 1443 837 274 195 138
80 51 33 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8190 8222 8190
Size: 32 8198
Tth111I GACn'n_nGTC
Cuts at: 1316 2576 2852 4892 1316
Size: 1260 276 2040 4654
Fragments arranged by size:
4654 2040 1260 276
XbaI T'CTAG_A
Cuts at: 5566 5566
Size: 8230
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2381 2402 2424 2381
Size: 21 22 8187
Fragments arranged by size:
8187 22 21
XhoI C'TCGA_G
Cuts at: 1943 4355 4711 1943
Size: 2412 356 5462
Fragments arranged by size:
5462 2412 356
XmaI C'CCGG_G
Cuts at: 1870 3138 4272 5555 1870
Size: 1268 1134 1283 4545
Fragments arranged by size:
4545 1283 1268 1134
XmnI GAAnn'nnTTC
Cuts at: 4496 5462 6359 4496
Size: 966 897 6367
Fragments arranged by size:
6367 966 897
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2578 6038 276
Size: 53 83 186 936 1044 3460 2468
Fragments arranged by size:
3460 2468 1044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI
RsrII SacII SfiI SwaI