Restriction map for
pFN21AB9494
(Circular) (Six-base) MAPSORT of: pFN21AB9494.seq Check: 8686 from: 1 to: 7225
pFN21AB9494 7225 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2651 2829 4108 2651
Size: 178 1279 5768
Fragments arranged by size:
5768 1279 178
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5035 278
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4568 1937
Size: 2631 4594
Acc65I G'GTAC_C
Cuts at: 4546 4546
Size: 7225
AclI AA'CG_TT
Cuts at: 1563 5352 5725 1563
Size: 3789 373 3063
Fragments arranged by size:
3789 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3030 5288 6672 6940 1632
Size: 1398 2258 1384 268 1917
Fragments arranged by size:
2258 1917 1398 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7028
AflIII A'CryG_T
Cuts at: 1203 2607 6124 7101 1203
Size: 1404 3517 977 1327
Fragments arranged by size:
3517 1404 1327 977
AgeI A'CCGG_T
Cuts at: 6056 6056
Size: 7225
AhdI GACnn_n'nnGTC
Cuts at: 5954 5954
Size: 7225
AleI CACnn'nnGTG
Cuts at: 1166 2222 1166
Size: 1056 6169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7225
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2808 2840 2808
Size: 32 7193
AlwNI CAG_nnn'CTG
Cuts at: 3457 4294 4333 4360 4450 4453 4968 6540 3457
Size: 837 39 27 90 3 515 1572 4142
Fragments arranged by size:
4142 1572 837 515 90 39 27 3
ApaI G_GGCC'C
Cuts at: 2145 3517 2145
Size: 1372 5853
ApaLI G'TGCA_C
Cuts at: 4394 5282 6438 4394
Size: 888 1156 5181
Fragments arranged by size:
5181 1156 888
ApoI r'AATT_y
Cuts at: 1493 2780 3998 4534 4760 4912 7021 1493
Size: 1287 1218 536 226 152 2109 1697
Fragments arranged by size:
2109 1697 1287 1218 536 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5779 160
Size: 872 4747 1606
Fragments arranged by size:
4747 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 3017 3087 4211 4550 1870
Size: 73 137 937 70 1124 339 4545
Fragments arranged by size:
4545 1124 937 339 137 73 70
BamHI G'GATC_C
Cuts at: 4555 4922 4555
Size: 367 6858
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2169 2198 3043 3113 4029
Size: 325 217 627 382 29 845 70 916
Cuts at: 4029 4144 4514 4546 6001 618
Size: 115 370 32 1455 1842
Fragments arranged by size:
1842 1455 916 845 627 382 370 325
217 115 70 32 29
BanII G_rGCy'C
Cuts at: 1391 2145 2640 2834 3517 4544 1391
Size: 754 495 194 683 1027 4072
Fragments arranged by size:
4072 1027 754 683 495 194
BbeI G_GCGC'C
Cuts at: 2173 4518 2173
Size: 2345 4880
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2276 928
Size: 971 377 5877
Fragments arranged by size:
5877 971 377
BbvCI CC'TCA_GC
Cuts at: 3121 3121
Size: 7225
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5416 5450 1903
Size: 34 3479 34 3678
Fragments arranged by size:
3678 3479 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5117 6327 5117
Size: 1210 6015
BclI T'GATC_A
Cuts at: 1548 3837 1548
Size: 2289 4936
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2540 4049 4153
Size: 107 122 71 631 605 868 1509 104
Cuts at: 4153 5836 136
Size: 1683 1525
Fragments arranged by size:
1683 1525 1509 868 631 605 122 107
104 71
BglII A'GATC_T
Cuts at: 7220 7220
Size: 7225
BlpI GC'TnA_GC
Cuts at: 3776 4439 4642 3776
Size: 663 203 6359
Fragments arranged by size:
6359 663 203
Bme1580I G_kGCm'C
Cuts at: 1790 2145 2203 3018 3048 3221 3517 4032 4149
Size: 355 58 815 30 173 296 515 117
Cuts at: 4149 4398 5286 6442 1790
Size: 249 888 1156 2573
Fragments arranged by size:
2573 1156 888 815 515 355 296 249
173 117 58 30
BmgBI CAC'GTC
Cuts at: 1318 2796 2950 3337 1318
Size: 1478 154 387 5206
Fragments arranged by size:
5206 1478 387 154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2346 2424 3153
Size: 416 53 83 399 24 929 78 729
Cuts at: 3153 3470 4956 5914 442
Size: 317 1486 958 1753
Fragments arranged by size:
1753 1486 958 929 729 416 399 317
83 78 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7225
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3880 3880
Size: 7225
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2609 3479 3710 4001 4054 4316 4355 5885
Size: 1370 870 231 291 53 262 39 1530
Cuts at: 5885 1239
Size: 2579
Fragments arranged by size:
2579 1530 1370 870 291 262 231 53
39
Bpu10I CC'TnA_GC
Cuts at: 3121 3121
Size: 7225
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3770 5350 6215 6513 3770
Size: 1580 865 298 4482
Fragments arranged by size:
4482 1580 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4417 5888 882
Size: 617 2918 1471 2219
Fragments arranged by size:
2918 2219 1471 617
BsaAI yAC'GTr
Cuts at: 493 1204 3382 3535 5016 493
Size: 711 2178 153 1481 2702
Fragments arranged by size:
2702 2178 1481 711 153
BsaBI GATnn'nnATC
Cuts at: 7219 7219
Size: 7225
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2170 3198 4515 5032
Size: 53 83 186 936 637 1028 1317 517
Cuts at: 5032 5414 6049 275
Size: 382 635 1451
Fragments arranged by size:
1451 1317 1028 936 637 635 517 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4092 5658 6056 6330 6477 6888 4092
Size: 1566 398 274 147 411 4429
Fragments arranged by size:
4429 1566 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2808 2838 3683 3713 1178
Size: 30 165 30 1405 30 845 30 4690
Fragments arranged by size:
4690 1405 845 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 3053 3077 3377 3856 1186
Size: 1480 387 24 300 479 4555
Fragments arranged by size:
4555 1480 479 387 300 24
BseYI C'CCAG_C
Cuts at: 2646 2981 3138 3349 4148 4510 6428 6924 2646
Size: 335 157 211 799 362 1918 496 2947
Fragments arranged by size:
2947 1918 799 496 362 335 211 157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2341 2921 3275 3392 3515 4334 4367
Size: 573 605 580 354 117 123 819 33
Cuts at: 4367 1163
Size: 4021
Fragments arranged by size:
4021 819 605 580 573 354 123 117
33
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2373 2913 3521 4436 4675 5436
Size: 275 186 372 540 608 915 239 761
Cuts at: 5436 6464 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 915 761 608 540 372 275
239 186
BsiHKAI G_wGCw'C
Cuts at: 2640 3576 3795 4398 5286 5371 6442 2640
Size: 936 219 603 888 85 1071 3423
Fragments arranged by size:
3423 1071 936 888 603 219 85
BsmI GAATG_Cn'
Cuts at: 4736 4829 4736
Size: 93 7132
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2203 2640 2834 3018 3048 3221
Size: 399 355 58 437 194 184 30 173
Cuts at: 3221 3517 3576 3795 4032 4149 4398 4544 5286
Size: 296 59 219 237 117 249 146 742
Cuts at: 5286 5371 6442 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 437 399 355 296 249
237 219 194 184 173 146 117 85
59 58 30
BspEI T'CCGG_A
Cuts at: 6888 6888
Size: 7225
BspHI T'CATG_A
Cuts at: 3840 5114 6844 3840
Size: 1274 1730 4221
Fragments arranged by size:
4221 1730 1274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2651 2829 3738 4108 4580 844
Size: 1807 178 909 370 472 3489
Fragments arranged by size:
3489 1807 909 472 370 178
BsrBI CCG'CTC
Cuts at: 1394 1823 3067 3989 4068 5112 1394
Size: 429 1244 922 79 1044 3507
Fragments arranged by size:
3507 1244 1044 922 429 79
BsrDI GCAATG_nn'
Cuts at: 65 1248 5720 5894 65
Size: 1183 4472 174 1396
Fragments arranged by size:
4472 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2347 4266 5869 6056 1700
Size: 110 537 1919 1603 187 2869
Fragments arranged by size:
2869 1919 1603 537 187 110
BsrGI T'GTAC_A
Cuts at: 96 2550 3204 3252 3654 96
Size: 2454 654 48 402 3667
Fragments arranged by size:
3667 2454 654 402 48
BssHII G'CGCG_C
Cuts at: 1925 2061 1925
Size: 136 7089
BssSI C'ACGA_G
Cuts at: 3293 5285 6297 3293
Size: 1992 1012 4221
Fragments arranged by size:
4221 1992 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3739 4321 4381 4453 794
Size: 2945 582 60 72 3566
Fragments arranged by size:
3566 2945 582 72 60
BstBI TT'CG_AA
Cuts at: 2148 6029 2148
Size: 3881 3344
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7225
BstYI r'GATC_y
Cuts at: 1972 2568 2673 4329 4555 4922 5307 5324 6776
Size: 596 105 1656 226 367 385 17 1452
Cuts at: 6776 6862 6874 7220 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1656 1452 596 385 367 346 226
105 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2536 2544 2700 2868 3611 3723
Size: 552 939 532 8 156 168 743 112
Cuts at: 3723 4034 513
Size: 311 3704
Fragments arranged by size:
3704 939 743 552 532 311 168 156
112 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2144 487
Size: 683 56 918 5568
Fragments arranged by size:
5568 918 683 56
BtsI GCAGTG_nn'
Cuts at: 2938 3793 4375 4505 4741 5535 5555 2938
Size: 855 582 130 236 794 20 4608
Fragments arranged by size:
4608 855 794 582 236 130 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7225
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7190
DraI TTT'AAA
Cuts at: 4529 4529
Size: 7225
DraIII CAC_nnn'GTG
Cuts at: 2221 2221
Size: 7225
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6232 809
Size: 507 4916 1802
Fragments arranged by size:
4916 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2370 2482 2910
Size: 54 1425 50 275 18 540 112 428
Cuts at: 2910 3518 3537 4037 4059 4672 5561 7190 8
Size: 608 19 500 22 613 889 1629 43
Fragments arranged by size:
1629 1425 889 613 608 540 500 428
275 112 54 50 43 22 19 18
EagI C'GGCC_G
Cuts at: 1537 1812 2370 2910 3518 4672 1537
Size: 275 558 540 608 1154 4090
Fragments arranged by size:
4090 1154 608 558 540 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2774 3176 5155 1353
Size: 1421 402 1979 3423
Fragments arranged by size:
3423 1979 1421 402
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3910 5812 6186 6332 387
Size: 1064 138 2321 1902 374 146 1280
Fragments arranged by size:
2321 1902 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2638 2638
Size: 7225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 3030 3479 3710 4001 4054 4316
Size: 393 977 421 449 231 291 53 262
Cuts at: 4316 4355 5288 5885 6672 6940 1239
Size: 39 933 597 787 268 1524
Fragments arranged by size:
1524 977 933 787 597 449 421 393
291 268 262 231 53 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2320 2320
Size: 7225
EcoO109I rG'GnC_Cy
Cuts at: 2142 2325 2340 3490 3513 2142
Size: 183 15 1150 23 5854
Fragments arranged by size:
5854 1150 183 23 15
EcoRI G'AATT_C
Cuts at: 2780 3998 4534 4912 2780
Size: 1218 536 378 5093
Fragments arranged by size:
5093 1218 536 378
FspI TGC'GCA
Cuts at: 4504 5731 4504
Size: 1227 5998
HaeII r_GCGC'y
Cuts at: 1678 2173 2857 3862 4518 6372 1678
Size: 495 684 1005 656 1854 2531
Fragments arranged by size:
2531 1854 1005 684 656 495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5866 5898 5940 5972 1373
Size: 32 135 32 4294 32 42 32 2626
Fragments arranged by size:
4294 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4201 4569 4815 669
Size: 988 281 2263 368 246 3079
Fragments arranged by size:
3079 2263 988 368 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7225
HpaI GTT'AAC
Cuts at: 4815 4815
Size: 7225
KasI G'GCGC_C
Cuts at: 2169 4514 2169
Size: 2345 4880
KpnI G_GTAC'C
Cuts at: 4550 4550
Size: 7225
MfeI C'AATT_G
Cuts at: 2616 4824 2616
Size: 2208 5017
MluI A'CGCG_T
Cuts at: 7101 7101
Size: 7225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3012 6339 6523 1305
Size: 1707 3327 184 2007
Fragments arranged by size:
3327 2007 1707 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2484 3539 10
Size: 54 1425 343 652 1055 3696
Fragments arranged by size:
3696 1425 1055 652 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2892 2943 3650 4391 5183 5542
Size: 648 1056 670 51 707 741 792 359
Cuts at: 5542 5701 518
Size: 159 2042
Fragments arranged by size:
2042 1056 792 741 707 670 648 359
159 51
MspA1I CmG'CkG
Cuts at: 1606 2851 3243 3408 3697 3775 4343 4450 4641
Size: 1245 392 165 289 78 568 107 191
Cuts at: 4641 5318 6466 6711 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1245 1148 677 568 392 289 245
191 165 107 78
NarI GG'CG_CC
Cuts at: 2170 4515 2170
Size: 2345 4880
NcoI C'CATG_G
Cuts at: 513 1065 2004 2536 2868 513
Size: 552 939 532 332 4870
Fragments arranged by size:
4870 939 552 532 332
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7225
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7225
NotI GC'GGCC_GC
Cuts at: 4672 4672
Size: 7225
NspI r_CATG'y
Cuts at: 1132 1229 2395 2611 4207 4583 6128 1132
Size: 97 1166 216 1596 376 1545 2229
Fragments arranged by size:
2229 1596 1545 1166 376 216 97
PasI CC'CwG_GG
Cuts at: 1348 3483 1348
Size: 2135 5090
PciI A'CATG_T
Cuts at: 2607 6124 2607
Size: 3517 3708
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3457 3937 1108
Size: 2349 480 4396
Fragments arranged by size:
4396 2349 480
PfoI T'CCnGG_A
Cuts at: 2625 3543 3960 4332 2625
Size: 918 417 372 5518
Fragments arranged by size:
5518 918 417 372
PmeI GTTT'AAAC
Cuts at: 4529 4529
Size: 7225
PmlI CAC'GTG
Cuts at: 3535 3535
Size: 7225
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2808 2840 5294 5326 6807 6839 2808
Size: 32 2454 32 1481 32 3194
Fragments arranged by size:
3194 2454 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2340 2340
Size: 7225
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7225
PsiI TTA'TAA
Cuts at: 4795 4795
Size: 7225
PspOMI G'GGCC_C
Cuts at: 2141 3513 2141
Size: 1372 5853
PstI C_TGCA'G
Cuts at: 830 3735 3774 4275 4329 4577 830
Size: 2905 39 501 54 248 3478
Fragments arranged by size:
3478 2905 501 248 54 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7225
PvuII CAG'CTG
Cuts at: 3697 3775 4343 4450 3697
Size: 78 568 107 6472
Fragments arranged by size:
6472 568 107 78
SacI G_AGCT'C
Cuts at: 2640 2640
Size: 7225
SalI G'TCGA_C
Cuts at: 1936 4567 1936
Size: 2631 4594
SanDI GG'GwC_CC
Cuts at: 2340 2340
Size: 7225
SapI GCTCTTCn'nnn_
Cuts at: 3176 3176
Size: 7225
SbfI CC_TGCA'GG
Cuts at: 4577 4577
Size: 7225
ScaI AGT'ACT
Cuts at: 5473 7185 5473
Size: 1712 5513
SexAI A'CCwGG_T
Cuts at: 3831 3831
Size: 7225
SfcI C'TryA_G
Cuts at: 826 1045 3731 3770 4271 4325 4573 5708 6389
Size: 219 2686 39 501 54 248 1135 681
Cuts at: 6389 6580 826
Size: 191 1471
Fragments arranged by size:
2686 1471 1135 681 501 248 219 191
54 39
SfoI GGC'GCC
Cuts at: 2171 4516 2171
Size: 2345 4880
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7225
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7225
SmaI CCC'GGG
Cuts at: 1872 4552 1872
Size: 2680 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3785 5329 6230 6492 820
Size: 197 926 1842 1544 901 262 1553
Fragments arranged by size:
1842 1553 1544 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5016 493
Size: 4523 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7225
SphI G_CATG'C
Cuts at: 1132 4583 1132
Size: 3451 3774
SspI AAT'ATT
Cuts at: 5 52 5149 5
Size: 47 5097 2081
Fragments arranged by size:
5097 2081 47
StuI AGG'CCT
Cuts at: 1517 3719 4160 1517
Size: 2202 441 4582
Fragments arranged by size:
4582 2202 441
StyI C'CwwG_G
Cuts at: 513 1065 2004 2070 2536 2868 3924 4407 4664
Size: 552 939 66 466 332 1056 483 257
Cuts at: 4664 513
Size: 3074
Fragments arranged by size:
3074 1056 939 552 483 466 332 257
66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2186 2646 3152 3341 4047 5252 5269
Size: 1255 606 460 506 189 706 1205 17
Cuts at: 5269 5422 5607 6052 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1205 706 606 506 460 445
189 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2550 3204 3252
Size: 274 80 33 51 1443 573 654 48
Cuts at: 3252 3654 3708 5471 7183 96
Size: 402 54 1763 1712 138
Fragments arranged by size:
1763 1712 1443 654 573 402 274 138
80 54 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2780 2812 3657 3683 3689 3715 7185 7217 2780
Size: 32 845 26 6 26 3470 32 2788
Fragments arranged by size:
3470 2788 845 32 32 26 26 6
Tth111I GACn'n_nGTC
Cuts at: 1316 2114 1316
Size: 798 6427
XbaI T'CTAG_A
Cuts at: 4561 4561
Size: 7225
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4138 4138
Size: 7225
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7225
XmaI C'CCGG_G
Cuts at: 1870 4550 1870
Size: 2680 4545
XmnI GAAnn'nnTTC
Cuts at: 3851 5354 3851
Size: 1503 5722
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5033 276
Size: 53 83 186 936 3499 2468
Fragments arranged by size:
3499 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI
BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacII SfiI
SrfI SwaI