Restriction map for
pFN21AB9492
(Circular) (Six-base) MAPSORT of: pFN21AB9492.seq Check: 480 from: 1 to: 8314
pFN21AB9492 8314 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6124 278
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3466 5657 1937
Size: 1529 2191 4594
Fragments arranged by size:
4594 2191 1529
Acc65I G'GTAC_C
Cuts at: 5635 5635
Size: 8314
AclI AA'CG_TT
Cuts at: 1563 6441 6814 1563
Size: 4878 373 3063
Fragments arranged by size:
4878 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2413 3181 3477 5507 6377 7761 8029 1632
Size: 781 768 296 2030 870 1384 268 1917
Fragments arranged by size:
2030 1917 1384 870 781 768 296 268
AflII C'TTAA_G
Cuts at: 820 1017 2162 820
Size: 197 1145 6972
Fragments arranged by size:
6972 1145 197
AflIII A'CryG_T
Cuts at: 1203 7213 8190 1203
Size: 6010 977 1327
Fragments arranged by size:
6010 1327 977
AgeI A'CCGG_T
Cuts at: 7145 7145
Size: 8314
AhdI GACnn_n'nnGTC
Cuts at: 7043 7043
Size: 8314
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8314
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8314
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5208 5240 5208
Size: 32 8282
AlwNI CAG_nnn'CTG
Cuts at: 5485 5596 6057 7629 5485
Size: 111 461 1572 6170
Fragments arranged by size:
6170 1572 461 111
ApaLI G'TGCA_C
Cuts at: 4029 6371 7527 4029
Size: 2342 1156 4816
Fragments arranged by size:
4816 2342 1156
ApoI r'AATT_y
Cuts at: 1493 2263 2673 3425 3550 3665 3698 4725 5623
Size: 770 410 752 125 115 33 1027 898
Cuts at: 5623 5849 6001 8110 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1027 898 770 752 410 226
152 125 115 33
AseI AT'TA_AT
Cuts at: 160 1032 2852 6868 160
Size: 872 1820 4016 1606
Fragments arranged by size:
4016 1820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
AvaI C'yCGr_G
Cuts at: 1870 1943 5639 1870
Size: 73 3696 4545
Fragments arranged by size:
4545 3696 73
BamHI G'GATC_C
Cuts at: 3040 5644 6011 3040
Size: 2604 367 5343
Fragments arranged by size:
5343 2604 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5635 7090 618
Size: 325 217 627 3848 1455 1842
Fragments arranged by size:
3848 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2161 3589 5633 1391
Size: 770 1428 2044 4072
Fragments arranged by size:
4072 2044 1428 770
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2832 3278 3978 4536 4868 928
Size: 971 933 446 700 558 332 4374
Fragments arranged by size:
4374 971 933 700 558 446 332
BbvCI CC'TCA_GC
Cuts at: 4173 4173
Size: 8314
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4885 4919 6505 6539 1903
Size: 34 2948 34 1586 34 3678
Fragments arranged by size:
3678 2948 1586 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3819 6206 7416 3819
Size: 2387 1210 4717
Fragments arranged by size:
4717 2387 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8314
BfrBI ATG'CAT
Cuts at: 2880 3931 5088 2880
Size: 1051 1157 6106
Fragments arranged by size:
6106 1157 1051
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6925 136
Size: 107 122 71 631 605 5253 1525
Fragments arranged by size:
5253 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5011 8309 5011
Size: 3298 5016
BlpI GC'TnA_GC
Cuts at: 2496 5149 5731 2496
Size: 2653 582 5079
Fragments arranged by size:
5079 2653 582
Bme1580I G_kGCm'C
Cuts at: 1790 4033 4747 6375 7531 1790
Size: 2243 714 1628 1156 2573
Fragments arranged by size:
2573 2243 1628 1156 714
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8314
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2562 6045 7003
Size: 416 53 83 399 24 1145 3483 958
Cuts at: 7003 442
Size: 1753
Fragments arranged by size:
3483 1753 1145 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4133 1056
Size: 3077 5237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2075 2154 4874 6974 1239
Size: 836 79 2720 2100 2579
Fragments arranged by size:
2720 2579 2100 836 79
Bpu10I CC'TnA_GC
Cuts at: 4173 4173
Size: 8314
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3542 4212 5138 6439 7304 7602 3542
Size: 670 926 1301 865 298 4254
Fragments arranged by size:
4254 1301 926 865 670 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3785 6977 882
Size: 617 2286 3192 2219
Fragments arranged by size:
3192 2286 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6105 493
Size: 711 4901 2702
Fragments arranged by size:
4901 2702 711
BsaBI GATnn'nnATC
Cuts at: 4401 8308 4401
Size: 3907 4407
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6121 6503 7138 275
Size: 53 83 186 936 4588 382 635 1451
Fragments arranged by size:
4588 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4785 6747 7145 7419 7566 7977 4785
Size: 1962 398 274 147 411 5122
Fragments arranged by size:
5122 1962 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2388 2418 2574 2604 5208
Size: 30 165 30 985 30 156 30 2604
Cuts at: 5208 5238 1178
Size: 30 4254
Fragments arranged by size:
4254 2604 985 165 156 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2127 2130 5130 5133 5213 1186
Size: 941 3 3000 3 80 4287
Fragments arranged by size:
4287 3000 941 80 3 3
BseYI C'CCAG_C
Cuts at: 2059 2686 5346 7517 8013 2059
Size: 627 2660 2171 496 2360
Fragments arranged by size:
2660 2360 2171 627 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4713 4830 1163
Size: 573 2977 117 4647
Fragments arranged by size:
4647 2977 573 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5764 6525 7553 1540
Size: 275 186 3763 761 1028 2301
Fragments arranged by size:
3763 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4033 6375 6460 7531 4033
Size: 2342 85 1071 4816
Fragments arranged by size:
4816 2342 1071 85
BsmI GAATG_Cn'
Cuts at: 2232 2455 4936 5825 5918 2232
Size: 223 2481 889 93 4628
Fragments arranged by size:
4628 2481 889 223 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2161 3589 4033 4747 5633 6375 6460
Size: 399 371 1428 444 714 886 742 85
Cuts at: 6460 7531 1391
Size: 1071 2174
Fragments arranged by size:
2174 1428 1071 886 742 714 444 399
371 85
BspEI T'CCGG_A
Cuts at: 4785 7977 4785
Size: 3192 5122
BspHI T'CATG_A
Cuts at: 6203 7933 6203
Size: 1730 6584
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5301 5437 5669 844
Size: 4457 136 232 3489
Fragments arranged by size:
4457 3489 232 136
BsrBI CCG'CTC
Cuts at: 1394 1823 6201 1394
Size: 429 4378 3507
Fragments arranged by size:
4378 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3375 3385 4140 6809 6983 65
Size: 1183 2127 10 755 2669 174 1396
Fragments arranged by size:
2669 2127 1396 1183 755 174 10
BsrFI r'CCGG_y
Cuts at: 1700 1810 6958 7145 1700
Size: 110 5148 187 2869
Fragments arranged by size:
5148 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2304 3979 96
Size: 2208 1675 4431
Fragments arranged by size:
4431 2208 1675
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8314
BssSI C'ACGA_G
Cuts at: 6374 7386 6374
Size: 1012 7302
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3506 794
Size: 2712 5602
BstBI TT'CG_AA
Cuts at: 7118 7118
Size: 8314
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8314
BstXI CCAn_nnnn'nTGG
Cuts at: 2625 5384 2625
Size: 2759 5555
BstYI r'GATC_y
Cuts at: 1972 3011 3040 5011 5644 6011 6396 6413 7865
Size: 1039 29 1971 633 367 385 17 1452
Cuts at: 7865 7951 7963 8309 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1971 1452 1039 633 385 367 346
86 29 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3103 4614 513
Size: 552 939 1099 1511 4213
Fragments arranged by size:
4213 1511 1099 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7575
Fragments arranged by size:
7575 683 56
BtsI GCAGTG_nn'
Cuts at: 2362 2396 2981 5830 6624 6644 2362
Size: 34 585 2849 794 20 4032
Fragments arranged by size:
4032 2849 794 585 34 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8314
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8279
DraI TTT'AAA
Cuts at: 3220 5238 5618 3220
Size: 2018 380 5916
Fragments arranged by size:
5916 2018 380
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7321 809
Size: 507 6005 1802
Fragments arranged by size:
6005 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3912 5280 5761
Size: 54 1425 50 275 18 2082 1368 481
Cuts at: 5761 6650 8279 8
Size: 889 1629 43
Fragments arranged by size:
2082 1629 1425 1368 889 481 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5761 1537
Size: 275 3949 4090
Fragments arranged by size:
4090 3949 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3368 4055 4404 5186 6244 1353
Size: 2015 687 349 782 1058 3423
Fragments arranged by size:
3423 2015 1058 782 687 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5052 6901 7275 7421 387
Size: 1064 138 3463 1849 374 146 1280
Fragments arranged by size:
3463 1849 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2075 2154 2413 3181 3477 4874 5507
Size: 393 443 79 259 768 296 1397 633
Cuts at: 5507 6377 6974 7761 8029 1239
Size: 870 597 787 268 1524
Fragments arranged by size:
1524 1397 870 787 768 633 597 443
393 296 268 259 79
EcoNI CCTnn'n_nnAGG
Cuts at: 3219 3219
Size: 8314
EcoO109I rG'GnC_Cy
Cuts at: 5130 5509 5130
Size: 379 7935
EcoRI G'AATT_C
Cuts at: 2263 3698 4725 5623 6001 2263
Size: 1435 1027 898 378 4576
Fragments arranged by size:
4576 1435 1027 898 378
EcoRV GAT'ATC
Cuts at: 4216 4216
Size: 8314
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3265 3718 4896 3265
Size: 453 1178 6683
Fragments arranged by size:
6683 1178 453
FspI TGC'GCA
Cuts at: 6820 6820
Size: 8314
HaeII r_GCGC'y
Cuts at: 1678 4009 7461 1678
Size: 2331 3452 2531
Fragments arranged by size:
3452 2531 2331
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 3055 3087 3726
Size: 32 135 32 592 32 859 32 639
Cuts at: 3726 3758 3916 3948 6955 6987 7029 7061 1373
Size: 32 158 32 3007 32 42 32 2626
Fragments arranged by size:
3007 2626 859 639 592 158 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2104 5658 5904 669
Size: 988 281 166 3554 246 3079
Fragments arranged by size:
3554 3079 988 281 246 166
HindIII A'AGCT_T
Cuts at: 748 2609 748
Size: 1861 6453
HpaI GTT'AAC
Cuts at: 5904 5904
Size: 8314
KpnI G_GTAC'C
Cuts at: 5639 5639
Size: 8314
MfeI C'AATT_G
Cuts at: 3938 4946 5913 3938
Size: 1008 967 6339
Fragments arranged by size:
6339 1008 967
MluI A'CGCG_T
Cuts at: 8190 8190
Size: 8314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5200 7428 7612 1305
Size: 3895 2228 184 2007
Fragments arranged by size:
3895 2228 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3914 5282 10
Size: 54 1425 343 2082 1368 3042
Fragments arranged by size:
3042 2082 1425 1368 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2478 2623 2875 6272 6631 6790 518
Size: 648 1312 145 252 3397 359 159 2042
Fragments arranged by size:
3397 2042 1312 648 359 252 159 145
MspA1I CmG'CkG
Cuts at: 1606 2154 3503 4112 4705 5350 5730 6407 7555
Size: 548 1349 609 593 645 380 677 1148
Cuts at: 7555 7800 1606
Size: 245 2120
Fragments arranged by size:
2120 1349 1148 677 645 609 593 548
380 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3103 4614 513
Size: 552 939 1099 1511 4213
Fragments arranged by size:
4213 1511 1099 939 552
NdeI CA'TA_TG
Cuts at: 387 5002 387
Size: 4615 3699
NheI G'CTAG_C
Cuts at: 1052 4129 1052
Size: 3077 5237
NotI GC'GGCC_GC
Cuts at: 5761 5761
Size: 8314
NsiI A_TGCA'T
Cuts at: 2882 3933 5090 2882
Size: 1051 1157 6106
Fragments arranged by size:
6106 1157 1051
NspI r_CATG'y
Cuts at: 1132 1229 2686 3864 4144 4817 4900 5672 7217
Size: 97 1457 1178 280 673 83 772 1545
Cuts at: 7217 1132
Size: 2229
Fragments arranged by size:
2229 1545 1457 1178 772 673 280 97
83
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8314
PciI A'CATG_T
Cuts at: 7213 7213
Size: 8314
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8314
PfoI T'CCnGG_A
Cuts at: 3681 5376 3681
Size: 1695 6619
PmeI GTTT'AAAC
Cuts at: 5618 5618
Size: 8314
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5208 5240 6383 6415 7896 7928 5208
Size: 32 1143 32 1481 32 5594
Fragments arranged by size:
5594 1481 1143 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5130 5130
Size: 8314
PshAI GACnn'nnGTC
Cuts at: 1613 2865 1613
Size: 1252 7062
PsiI TTA'TAA
Cuts at: 5884 5884
Size: 8314
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5282 5314 5282
Size: 32 8282
PstI C_TGCA'G
Cuts at: 830 2153 4021 4117 4704 5666 830
Size: 1323 1868 96 587 962 3478
Fragments arranged by size:
3478 1868 1323 962 587 96
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8314
PvuII CAG'CTG
Cuts at: 3503 4112 4705 5350 3503
Size: 609 593 645 6467
Fragments arranged by size:
6467 645 609 593
SalI G'TCGA_C
Cuts at: 1936 5656 1936
Size: 3720 4594
SapI GCTCTTCn'nnn_
Cuts at: 3368 3368
Size: 8314
SbfI CC_TGCA'GG
Cuts at: 5666 5666
Size: 8314
ScaI AGT'ACT
Cuts at: 6562 8274 6562
Size: 1712 6602
SexAI A'CCwGG_T
Cuts at: 5403 5403
Size: 8314
SfcI C'TryA_G
Cuts at: 826 1045 2149 3467 4017 4113 4700 5542 5662
Size: 219 1104 1318 550 96 587 842 120
Cuts at: 5662 6797 7478 7669 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1318 1135 1104 842 681 587 550
219 191 120 96
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8314
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8314
SmaI CCC'GGG
Cuts at: 1872 5641 1872
Size: 3769 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2162 3557 4227 5117 6418 7319
Size: 197 926 219 1395 670 890 1301 901
Cuts at: 7319 7581 820
Size: 262 1553
Fragments arranged by size:
1553 1395 1301 926 901 890 670 262
219 197
SnaBI TAC'GTA
Cuts at: 493 6105 493
Size: 5612 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8314
SphI G_CATG'C
Cuts at: 1132 5672 1132
Size: 4540 3774
SspI AAT'ATT
Cuts at: 5 52 2226 2380 4514 6238 5
Size: 47 2174 154 2134 1724 2081
Fragments arranged by size:
2174 2134 2081 1724 154 47
StuI AGG'CCT
Cuts at: 1517 3225 1517
Size: 1708 6606
StyI C'CwwG_G
Cuts at: 513 1065 2004 2217 3103 3392 4614 4629 4639
Size: 552 939 213 886 289 1222 15 10
Cuts at: 4639 5512 5563 5753 513
Size: 873 51 190 3074
Fragments arranged by size:
3074 1222 939 886 873 552 289 213
190 51 15 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2678 3018 6341 6358 6511 6696 7141
Size: 1255 1098 340 3323 17 153 185 445
Cuts at: 7141 325
Size: 1498
Fragments arranged by size:
3323 1498 1255 1098 445 340 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2304 2395 2443
Size: 274 80 33 51 1443 327 91 48
Cuts at: 2443 3979 4428 6560 8272 96
Size: 1536 449 2132 1712 138
Fragments arranged by size:
2132 1712 1536 1443 449 327 274 138
91 80 51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4327 4359 4716 4748 8274 8306 4327
Size: 32 357 32 3526 32 4335
Fragments arranged by size:
4335 3526 357 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8314
XbaI T'CTAG_A
Cuts at: 2270 2914 5650 2270
Size: 644 2736 4934
Fragments arranged by size:
4934 2736 644
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8314
XmaI C'CCGG_G
Cuts at: 1870 5639 1870
Size: 3769 4545
XmnI GAAnn'nnTTC
Cuts at: 2280 3418 6443 2280
Size: 1138 3025 4151
Fragments arranged by size:
4151 3025 1138
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6122 276
Size: 53 83 186 936 4588 2468
Fragments arranged by size:
4588 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI