Restriction map for
pFN21AB9491
(Circular) (Six-base) MAPSORT of: pFN21AB9491.seq Check: 6238 from: 1 to: 8125
pFN21AB9491 8125 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5935 278
Size: 53 83 186 936 4399 2468
Fragments arranged by size:
4399 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5468 1937
Size: 3531 4594
Acc65I G'GTAC_C
Cuts at: 5446 5446
Size: 8125
AclI AA'CG_TT
Cuts at: 1563 3115 6252 6625 1563
Size: 1552 3137 373 3063
Fragments arranged by size:
3137 3063 1552 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2966 3117 3716 3737 4397 6188 7572 7840
Size: 1334 151 599 21 660 1791 1384 268
Cuts at: 7840 1632
Size: 1917
Fragments arranged by size:
1917 1791 1384 1334 660 599 268 151
21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7928
AflIII A'CryG_T
Cuts at: 1203 4255 7024 8001 1203
Size: 3052 2769 977 1327
Fragments arranged by size:
3052 2769 1327 977
AgeI A'CCGG_T
Cuts at: 6956 6956
Size: 8125
AhdI GACnn_n'nnGTC
Cuts at: 6854 6854
Size: 8125
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8125
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2220 2445 1184
Size: 1036 225 6864
Fragments arranged by size:
6864 1036 225
AlwNI CAG_nnn'CTG
Cuts at: 4114 5868 7440 4114
Size: 1754 1572 4799
Fragments arranged by size:
4799 1754 1572
ApaI G_GGCC'C
Cuts at: 5331 5331
Size: 8125
ApaLI G'TGCA_C
Cuts at: 6182 7338 6182
Size: 1156 6969
ApoI r'AATT_y
Cuts at: 1493 2060 2241 2250 2387 2495 2873 4718 4734
Size: 567 181 9 137 108 378 1845 16
Cuts at: 4734 5434 5660 5812 7921 1493
Size: 700 226 152 2109 1697
Fragments arranged by size:
2109 1845 1697 700 567 378 226 181
152 137 108 16 9
AseI AT'TA_AT
Cuts at: 160 1032 5127 6679 160
Size: 872 4095 1552 1606
Fragments arranged by size:
4095 1606 1552 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8125
AvaI C'yCGr_G
Cuts at: 1870 1943 2014 5450 1870
Size: 73 71 3436 4545
Fragments arranged by size:
4545 3436 73 71
BamHI G'GATC_C
Cuts at: 5248 5455 5822 5248
Size: 207 367 7551
Fragments arranged by size:
7551 367 207
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5446 6901 618
Size: 325 217 627 3659 1455 1842
Fragments arranged by size:
3659 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4309 5331 5444 1391
Size: 2918 1022 113 4072
Fragments arranged by size:
4072 2918 1022 113
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2574 2951 3636 3722 4588 928
Size: 971 675 377 685 86 866 4465
Fragments arranged by size:
4465 971 866 685 675 377 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2128 2162 6316 6350 1903
Size: 34 191 34 4154 34 3678
Fragments arranged by size:
4154 3678 191 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6017 7227 6017
Size: 1210 6915
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8125
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6736 136
Size: 107 122 71 631 605 5064 1525
Fragments arranged by size:
5064 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3136 4078 4615 8120 3136
Size: 942 537 3505 3141
Fragments arranged by size:
3505 3141 942 537
BlpI GC'TnA_GC
Cuts at: 5139 5542 5139
Size: 403 7722
Bme1580I G_kGCm'C
Cuts at: 1790 2100 4702 5331 6186 7342 1790
Size: 310 2602 629 855 1156 2573
Fragments arranged by size:
2602 2573 1156 855 629 310
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4126 4159 5856
Size: 416 53 83 399 24 2709 33 1697
Cuts at: 5856 6814 442
Size: 958 1753
Fragments arranged by size:
2709 1753 1697 958 416 399 83 53
33 24
BmtI G_CTAG'C
Cuts at: 1056 3101 1056
Size: 2045 6080
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3264 5367 3264
Size: 2103 6022
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3350 4136 5250 5352 5364 6785 1239
Size: 2111 786 1114 102 12 1421 2579
Fragments arranged by size:
2579 2111 1421 1114 786 102 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2726 4874 5089 6250 7115 7413 2726
Size: 2148 215 1161 865 298 3438
Fragments arranged by size:
3438 2148 1161 865 298 215
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3785 5007 6788 882
Size: 617 2286 1222 1781 2219
Fragments arranged by size:
2286 2219 1781 1222 617
BsaAI yAC'GTr
Cuts at: 493 1204 5916 493
Size: 711 4712 2702
Fragments arranged by size:
4712 2702 711
BsaBI GATnn'nnATC
Cuts at: 3585 8119 3585
Size: 4534 3591
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5932 6314 6949 275
Size: 53 83 186 936 4399 382 635 1451
Fragments arranged by size:
4399 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4389 6558 6956 7230 7377 7788 4389
Size: 2169 398 274 147 411 4726
Fragments arranged by size:
4726 2169 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2672 2702 1178
Size: 30 165 30 1269 30 6601
Fragments arranged by size:
6601 1269 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2318 2723 3428 4229 1186
Size: 1132 405 705 801 5082
Fragments arranged by size:
5082 1132 801 705 405
BseYI C'CCAG_C
Cuts at: 2093 2151 2439 5388 7328 7824 2093
Size: 58 288 2949 1940 496 2394
Fragments arranged by size:
2949 2394 1940 496 288 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2451 1163
Size: 573 715 6837
Fragments arranged by size:
6837 715 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5575 6336 7364 1540
Size: 275 186 3574 761 1028 2301
Fragments arranged by size:
3574 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4309 4599 5206 6186 6271 7342 4309
Size: 290 607 980 85 1071 5092
Fragments arranged by size:
5092 1071 980 607 290 85
BsmI GAATG_Cn'
Cuts at: 3171 4333 5636 5729 3171
Size: 1162 1303 93 5567
Fragments arranged by size:
5567 1303 1162 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 4309 4599 4702 5206 5331 5444
Size: 399 310 2209 290 103 504 125 113
Cuts at: 5444 6186 6271 7342 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2209 2174 1071 742 504 399 310 290
125 113 103 85
BspEI T'CCGG_A
Cuts at: 4389 7788 4389
Size: 3399 4726
BspHI T'CATG_A
Cuts at: 2230 4684 4821 6014 7744 2230
Size: 2454 137 1193 1730 2611
Fragments arranged by size:
2611 2454 1730 1193 137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3402 5480 844
Size: 2558 2078 3489
Fragments arranged by size:
3489 2558 2078
BsrBI CCG'CTC
Cuts at: 1394 1823 6012 1394
Size: 429 4189 3507
Fragments arranged by size:
4189 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4011 5233 6620 6794 65
Size: 1183 2763 1222 1387 174 1396
Fragments arranged by size:
2763 1396 1387 1222 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 6769 6956 1700
Size: 110 264 4695 187 2869
Fragments arranged by size:
4695 2869 264 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8125
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8125
BssSI C'ACGA_G
Cuts at: 6185 7197 6185
Size: 1012 7113
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4156 794
Size: 3362 4763
BstBI TT'CG_AA
Cuts at: 6929 6929
Size: 8125
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8125
BstXI CCAn_nnnn'nTGG
Cuts at: 4714 4714
Size: 8125
BstYI r'GATC_y
Cuts at: 1972 3136 4078 4615 5248 5383 5455 5822 6207
Size: 1164 942 537 633 135 72 367 385
Cuts at: 6207 6224 7676 7762 7774 8120 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1164 942 633 537 385 367
346 135 86 72 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6634
Fragments arranged by size:
6634 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7386
Fragments arranged by size:
7386 683 56
BtsI GCAGTG_nn'
Cuts at: 5641 6435 6455 5641
Size: 794 20 7311
Fragments arranged by size:
7311 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8090
DraI TTT'AAA
Cuts at: 2178 4791 5429 2178
Size: 2613 638 4874
Fragments arranged by size:
4874 2613 638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7132 809
Size: 507 5816 1802
Fragments arranged by size:
5816 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2055 2076 3661
Size: 54 1425 50 275 18 225 21 1585
Cuts at: 3661 5572 6461 8090 8
Size: 1911 889 1629 43
Fragments arranged by size:
1911 1629 1585 1425 889 275 225 54
50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 5572 1537
Size: 275 3760 4090
Fragments arranged by size:
4090 3760 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2475 3252 3297 3405 3570 3873 3960 3981
Size: 1122 777 45 108 165 303 87 21
Cuts at: 3981 4275 4425 6055 1353
Size: 294 150 1630 3423
Fragments arranged by size:
3423 1630 1122 777 303 294 165 150
108 87 45 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6712 7086 7232 387
Size: 1064 138 5123 374 146 1280
Fragments arranged by size:
5123 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4307 4307
Size: 8125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2966 3117 3350 3716 3737 4136 4397
Size: 393 1334 151 233 366 21 399 261
Cuts at: 4397 5250 5352 5364 6188 6785 7572 7840 1239
Size: 853 102 12 824 597 787 268 1524
Fragments arranged by size:
1524 1334 853 824 787 597 399 393
366 268 261 233 151 102 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3575 3575
Size: 8125
EcoO109I rG'GnC_Cy
Cuts at: 5327 5327
Size: 8125
EcoRI G'AATT_C
Cuts at: 5434 5812 5434
Size: 378 7747
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2879 5018 2879
Size: 2139 5986
FspI TGC'GCA
Cuts at: 6631 6631
Size: 8125
HaeII r_GCGC'y
Cuts at: 1678 5395 7272 1678
Size: 3717 1877 2531
Fragments arranged by size:
3717 2531 1877
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2186 2218 2672 2704 3200
Size: 32 135 32 614 32 454 32 496
Cuts at: 3200 3230 3232 3262 3919 3951 3983 4015 4334
Size: 30 2 30 657 32 32 32 319
Cuts at: 4334 4366 5004 5036 6766 6798 6840 6872 1373
Size: 32 638 32 1730 32 42 32 2626
Fragments arranged by size:
2626 1730 657 638 614 496 454 319
135 42 32 32 32 32 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 669 1657 1938 5469 5715 669
Size: 988 281 3531 246 3079
Fragments arranged by size:
3531 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2394 2978 3129 5361 748
Size: 1646 584 151 2232 3512
Fragments arranged by size:
3512 2232 1646 584 151
HpaI GTT'AAC
Cuts at: 5715 5715
Size: 8125
KpnI G_GTAC'C
Cuts at: 5450 5450
Size: 8125
MfeI C'AATT_G
Cuts at: 3483 3858 4500 5724 3483
Size: 375 642 1224 5884
Fragments arranged by size:
5884 1224 642 375
MluI A'CGCG_T
Cuts at: 8001 8001
Size: 8125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2473 4794 7239 7423 1305
Size: 1168 2321 2445 184 2007
Fragments arranged by size:
2445 2321 2007 1168 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3663 10
Size: 54 1425 343 1831 4472
Fragments arranged by size:
4472 1831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2363 3501 4488 4683 6083 6442 6601
Size: 648 1197 1138 987 195 1400 359 159
Cuts at: 6601 518
Size: 2042
Fragments arranged by size:
2042 1400 1197 1138 987 648 359 195
159
MspA1I CmG'CkG
Cuts at: 1606 2089 3104 4186 4496 4541 5059 5541 6218
Size: 483 1015 1082 310 45 518 482 677
Cuts at: 6218 7366 7611 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 1082 1015 677 518 483 482
310 245 45
NaeI GCC'GGC
Cuts at: 2076 2076
Size: 8125
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 6634
Fragments arranged by size:
6634 939 552
NdeI CA'TA_TG
Cuts at: 387 3477 4601 387
Size: 3090 1124 3911
Fragments arranged by size:
3911 3090 1124
NgoMIV G'CCGG_C
Cuts at: 2074 2074
Size: 8125
NheI G'CTAG_C
Cuts at: 1052 3097 1052
Size: 2045 6080
NotI GC'GGCC_GC
Cuts at: 5572 5572
Size: 8125
NspI r_CATG'y
Cuts at: 1132 1229 2422 2447 4259 4493 5483 7028 1132
Size: 97 1193 25 1812 234 990 1545 2229
Fragments arranged by size:
2229 1812 1545 1193 990 234 97 25
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8125
PciI A'CATG_T
Cuts at: 4255 7024 4255
Size: 2769 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3295 3676 1108
Size: 2187 381 5557
Fragments arranged by size:
5557 2187 381
PmeI GTTT'AAAC
Cuts at: 5429 5429
Size: 8125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6194 6226 7707 7739 6194
Size: 32 1481 32 6580
Fragments arranged by size:
6580 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8125
PsiI TTA'TAA
Cuts at: 2400 5695 2400
Size: 3295 4830
PspOMI G'GGCC_C
Cuts at: 5327 5327
Size: 8125
PstI C_TGCA'G
Cuts at: 830 3712 4342 5477 830
Size: 2882 630 1135 3478
Fragments arranged by size:
3478 2882 1135 630
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8125
PvuII CAG'CTG
Cuts at: 2089 3104 4496 4541 5059 2089
Size: 1015 1392 45 518 5155
Fragments arranged by size:
5155 1392 1015 518 45
SacI G_AGCT'C
Cuts at: 4309 4309
Size: 8125
SalI G'TCGA_C
Cuts at: 1936 5467 1936
Size: 3531 4594
SapI GCTCTTCn'nnn_
Cuts at: 3297 3297
Size: 8125
SbfI CC_TGCA'GG
Cuts at: 5477 5477
Size: 8125
ScaI AGT'ACT
Cuts at: 3624 6373 8085 3624
Size: 2749 1712 3664
Fragments arranged by size:
3664 2749 1712
SfcI C'TryA_G
Cuts at: 826 1045 2110 2834 2907 3708 4338 5473 6608
Size: 219 1065 724 73 801 630 1135 1135
Cuts at: 6608 7289 7480 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1135 1065 801 724 681 630
219 191 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8125
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8125
SmaI CCC'GGG
Cuts at: 1872 2016 5452 1872
Size: 144 3436 4545
Fragments arranged by size:
4545 3436 144
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2705 4889 5104 6229 7130 7392
Size: 197 926 762 2184 215 1125 901 262
Cuts at: 7392 820
Size: 1553
Fragments arranged by size:
2184 1553 1125 926 901 762 262 215
197
SnaBI TAC'GTA
Cuts at: 493 5916 493
Size: 5423 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8125
SphI G_CATG'C
Cuts at: 1132 5483 1132
Size: 4351 3774
SspI AAT'ATT
Cuts at: 5 52 2277 3371 6049 5
Size: 47 2225 1094 2678 2081
Fragments arranged by size:
2678 2225 2081 1094 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8125
StyI C'CwwG_G
Cuts at: 513 1065 2004 2160 3045 3795 5564 513
Size: 552 939 156 885 750 1769 3074
Fragments arranged by size:
3074 1769 939 885 750 552 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6152 6169 6322 6507 6952 325
Size: 1255 4572 17 153 185 445 1498
Fragments arranged by size:
4572 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2533 3622 6371
Size: 274 80 33 51 1443 556 1089 2749
Cuts at: 6371 8083 96
Size: 1712 138
Fragments arranged by size:
2749 1712 1443 1089 556 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8085 8117 8085
Size: 32 8093
Tth111I GACn'n_nGTC
Cuts at: 1316 2464 1316
Size: 1148 6977
XbaI T'CTAG_A
Cuts at: 2468 3322 5461 2468
Size: 854 2139 5132
Fragments arranged by size:
5132 2139 854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2086
Size: 8125
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8125
XmaI C'CCGG_G
Cuts at: 1870 2014 5450 1870
Size: 144 3436 4545
Fragments arranged by size:
4545 3436 144
XmnI GAAnn'nnTTC
Cuts at: 2241 3750 6254 2241
Size: 1509 2504 4112
Fragments arranged by size:
4112 2504 1509
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5933 276
Size: 53 83 186 936 4399 2468
Fragments arranged by size:
4399 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI