Restriction map for
pFN21AB9488
(Circular) (Six-base) MAPSORT of: pFN21AB9488.seq Check: 7435 from: 1 to: 8104
pFN21AB9488 8104 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5914 278
Size: 53 83 186 936 4378 2468
Fragments arranged by size:
4378 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2200 5371 5447 1937
Size: 263 3171 76 4594
Fragments arranged by size:
4594 3171 263 76
Acc65I G'GTAC_C
Cuts at: 5425 5425
Size: 8104
AclI AA'CG_TT
Cuts at: 1563 6231 6604 1563
Size: 4668 373 3063
Fragments arranged by size:
4668 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2451 2686 2756 3287 3524 3857 4044 4050
Size: 819 235 70 531 237 333 187 6
Cuts at: 4050 6167 7551 7819 1632
Size: 2117 1384 268 1917
Fragments arranged by size:
2117 1917 1384 819 531 333 268 237
235 187 70 6
AflII C'TTAA_G
Cuts at: 820 1017 4756 820
Size: 197 3739 4168
Fragments arranged by size:
4168 3739 197
AflIII A'CryG_T
Cuts at: 1203 7003 7980 1203
Size: 5800 977 1327
Fragments arranged by size:
5800 1327 977
AgeI A'CCGG_T
Cuts at: 6935 6935
Size: 8104
AhdI GACnn_n'nnGTC
Cuts at: 3595 6833 3595
Size: 3238 4866
AleI CACnn'nnGTG
Cuts at: 1166 2363 1166
Size: 1197 6907
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2503 1184
Size: 1319 6785
AlwNI CAG_nnn'CTG
Cuts at: 3694 5272 5847 7419 3694
Size: 1578 575 1572 4379
Fragments arranged by size:
4379 1578 1572 575
ApaI G_GGCC'C
Cuts at: 4777 4777
Size: 8104
ApaLI G'TGCA_C
Cuts at: 6161 7317 6161
Size: 1156 6948
ApoI r'AATT_y
Cuts at: 1493 2639 3249 3708 4682 5145 5413 5639 5791
Size: 1146 610 459 974 463 268 226 152
Cuts at: 5791 7900 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1146 974 610 463 459 268
226 152
AseI AT'TA_AT
Cuts at: 160 1032 6658 160
Size: 872 5626 1606
Fragments arranged by size:
5626 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8104
AvaI C'yCGr_G
Cuts at: 1870 1943 4835 5429 1870
Size: 73 2892 594 4545
Fragments arranged by size:
4545 2892 594 73
BamHI G'GATC_C
Cuts at: 5434 5801 5434
Size: 367 7737
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2281 5425 6880 618
Size: 325 217 627 494 3144 1455 1842
Fragments arranged by size:
3144 1842 1455 627 494 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 2782 4777 4800 5160 5423 1391
Size: 621 770 1995 23 360 263 4072
Fragments arranged by size:
4072 1995 770 621 360 263 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4421 4889 928
Size: 971 2522 468 4143
Fragments arranged by size:
4143 2522 971 468
BbvCI CC'TCA_GC
Cuts at: 4972 4972
Size: 8104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6295 6329 1903
Size: 34 4358 34 3678
Fragments arranged by size:
4358 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2199 3141 5216 5255 5996 7206 2199
Size: 942 2075 39 741 1210 3097
Fragments arranged by size:
3097 2075 1210 942 741 39
BclI T'GATC_A
Cuts at: 1548 4242 1548
Size: 2694 5410
BfrBI ATG'CAT
Cuts at: 2495 2954 2495
Size: 459 7645
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4157 6715 136
Size: 107 122 71 631 605 2485 2558 1525
Fragments arranged by size:
2558 2485 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2835 5059 8099 2835
Size: 2224 3040 2840
Fragments arranged by size:
3040 2840 2224
BlpI GC'TnA_GC
Cuts at: 5521 5521
Size: 8104
Bme1580I G_kGCm'C
Cuts at: 1790 3350 4777 6165 7321 1790
Size: 1560 1427 1388 1156 2573
Fragments arranged by size:
2573 1560 1427 1388 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8104
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2326 4219 5154
Size: 416 53 83 399 24 909 1893 935
Cuts at: 5154 5835 6793 442
Size: 681 958 1753
Fragments arranged by size:
1893 1753 958 935 909 681 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4784 4890 6764 1239
Size: 3545 106 1874 2579
Fragments arranged by size:
3545 2579 1874 106
Bpu10I CC'TnA_GC
Cuts at: 4185 4972 5327 4185
Size: 787 355 6962
Fragments arranged by size:
6962 787 355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3948 4827 6229 7094 7392 3948
Size: 879 1402 865 298 4660
Fragments arranged by size:
4660 1402 879 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5189 6767 882
Size: 617 3690 1578 2219
Fragments arranged by size:
3690 2219 1578 617
BsaAI yAC'GTr
Cuts at: 493 1204 4423 5023 5204 5895 493
Size: 711 3219 600 181 691 2702
Fragments arranged by size:
3219 2702 711 691 600 181
BsaBI GATnn'nnATC
Cuts at: 3831 8098 3831
Size: 4267 3837
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2118 5911 6293 6928
Size: 53 83 186 936 585 3793 382 635
Cuts at: 6928 275
Size: 1451
Fragments arranged by size:
3793 1451 936 635 585 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6537 6935 7209 7356 7767 6537
Size: 398 274 147 411 6874
Fragments arranged by size:
6874 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2442 2472 5193 5223 1178
Size: 30 165 30 1039 30 2721 30 4059
Fragments arranged by size:
4059 2721 1039 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4134 4137 4140 1186
Size: 2948 3 3 5150
Fragments arranged by size:
5150 2948 3 3
BseYI C'CCAG_C
Cuts at: 7307 7803 7307
Size: 496 7608
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2949 3788 4278 1163
Size: 573 1213 839 490 4989
Fragments arranged by size:
4989 1213 839 573 490
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5554 6315 7343 1540
Size: 275 186 3553 761 1028 2301
Fragments arranged by size:
3553 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2172 3512 4707 4800 6165 6250 7321 2172
Size: 1340 1195 93 1365 85 1071 2955
Fragments arranged by size:
2955 1365 1340 1195 1071 93 85
BsmI GAATG_Cn'
Cuts at: 4892 5059 5615 5708 4892
Size: 167 556 93 7288
Fragments arranged by size:
7288 556 167 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4079 4079
Size: 8104
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2172 2782 3350 3512 4707 4777
Size: 399 222 160 610 568 162 1195 70
Cuts at: 4777 4800 5160 5423 6165 6250 7321 1391
Size: 23 360 263 742 85 1071 2174
Fragments arranged by size:
2174 1195 1071 742 610 568 399 360
263 222 162 160 85 70 23
BspEI T'CCGG_A
Cuts at: 7767 7767
Size: 8104
BspHI T'CATG_A
Cuts at: 5993 7723 5993
Size: 1730 6374
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5459 844
Size: 4615 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3082 5991 1394
Size: 429 1259 2909 3507
Fragments arranged by size:
3507 2909 1259 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2460 2497 3322 4087 4983 6599 6773
Size: 1183 1212 37 825 765 896 1616 174
Cuts at: 6773 65
Size: 1396
Fragments arranged by size:
1616 1396 1212 1183 896 825 765 174
37
BsrFI r'CCGG_y
Cuts at: 1700 1810 2046 6748 6935 1700
Size: 110 236 4702 187 2869
Fragments arranged by size:
4702 2869 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 2764 4270 96
Size: 2668 1506 3930
Fragments arranged by size:
3930 2668 1506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8104
BssSI C'ACGA_G
Cuts at: 6164 7176 6164
Size: 1012 7092
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3071 5230 794
Size: 2277 2159 3668
Fragments arranged by size:
3668 2277 2159
BstBI TT'CG_AA
Cuts at: 6908 6908
Size: 8104
BstEII G'GTnAC_C
Cuts at: 1184 2177 1184
Size: 993 7111
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2981 2365
Size: 616 7488
BstYI r'GATC_y
Cuts at: 1972 2241 2835 4066 5059 5434 5801 6186 6203
Size: 269 594 1231 993 375 367 385 17
Cuts at: 6203 7655 7741 7753 8099 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1231 993 594 385 375 367
346 269 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7365
Fragments arranged by size:
7365 683 56
BtsI GCAGTG_nn'
Cuts at: 2418 3776 5620 6414 6434 2418
Size: 1358 1844 794 20 4088
Fragments arranged by size:
4088 1844 1358 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8069
DraI TTT'AAA
Cuts at: 4107 5408 4107
Size: 1301 6803
DraIII CAC_nnn'GTG
Cuts at: 2019 2548 2019
Size: 529 7575
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4333 7111 809
Size: 507 3017 2778 1802
Fragments arranged by size:
3017 2778 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5551 6440 8069
Size: 54 1425 50 275 18 3721 889 1629
Cuts at: 8069 8
Size: 43
Fragments arranged by size:
3721 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5551 1537
Size: 275 3739 4090
Fragments arranged by size:
4090 3739 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2614 3312 4434 6034 1353
Size: 1261 698 1122 1600 3423
Fragments arranged by size:
3423 1600 1261 1122 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6691 7065 7211 387
Size: 1064 138 5102 374 146 1280
Fragments arranged by size:
5102 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4798 4798
Size: 8104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2686 2756 3287 3524 3857 4044
Size: 393 819 235 70 531 237 333 187
Cuts at: 4044 4050 4784 4890 6167 6764 7551 7819 1239
Size: 6 734 106 1277 597 787 268 1524
Fragments arranged by size:
1524 1277 819 787 734 597 531 393
333 268 237 235 187 106 70 6
EcoO109I rG'GnC_Cy
Cuts at: 4774 4968 4774
Size: 194 7910
EcoRI G'AATT_C
Cuts at: 3249 5413 5791 3249
Size: 2164 378 5562
Fragments arranged by size:
5562 2164 378
EcoRV GAT'ATC
Cuts at: 3667 3667
Size: 8104
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5143 5143
Size: 8104
FspI TGC'GCA
Cuts at: 6610 6610
Size: 8104
HaeII r_GCGC'y
Cuts at: 1678 7251 1678
Size: 5573 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 2442 2474 3581
Size: 32 135 32 556 32 282 32 1107
Cuts at: 3581 3613 5051 5083 5190 5222 5372 5404 6745
Size: 32 1438 32 107 32 150 32 1341
Cuts at: 6745 6777 6819 6851 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1438 1341 1107 556 282 150 135
107 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 5372 5448 5694 669
Size: 988 281 1080 2354 76 246 3079
Fragments arranged by size:
3079 2354 1080 988 281 246 76
HindIII A'AGCT_T
Cuts at: 748 3117 3417 748
Size: 2369 300 5435
Fragments arranged by size:
5435 2369 300
HpaI GTT'AAC
Cuts at: 3018 5694 3018
Size: 2676 5428
KpnI G_GTAC'C
Cuts at: 5429 5429
Size: 8104
MfeI C'AATT_G
Cuts at: 2877 4647 5703 2877
Size: 1770 1056 5278
Fragments arranged by size:
5278 1770 1056
MluI A'CGCG_T
Cuts at: 7980 7980
Size: 8104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4468 7218 7402 1305
Size: 3163 2750 184 2007
Fragments arranged by size:
3163 2750 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6282
Fragments arranged by size:
6282 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2221 2363 3807 4632 5052 6062 6421
Size: 648 1055 142 1444 825 420 1010 359
Cuts at: 6421 6580 518
Size: 159 2042
Fragments arranged by size:
2042 1444 1055 1010 825 648 420 359
159 142
MspA1I CmG'CkG
Cuts at: 1606 3265 4376 4513 5272 5323 5520 6197 7345
Size: 1659 1111 137 759 51 197 677 1148
Cuts at: 7345 7590 1606
Size: 245 2120
Fragments arranged by size:
2120 1659 1148 1111 759 677 245 197
137 51
NaeI GCC'GGC
Cuts at: 2048 2048
Size: 8104
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7552
NdeI CA'TA_TG
Cuts at: 387 2218 387
Size: 1831 6273
NgoMIV G'CCGG_C
Cuts at: 2046 2046
Size: 8104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8104
NotI GC'GGCC_GC
Cuts at: 5551 5551
Size: 8104
NsiI A_TGCA'T
Cuts at: 2497 2956 2497
Size: 459 7645
NspI r_CATG'y
Cuts at: 1132 1229 2499 2591 5462 7007 1132
Size: 97 1270 92 2871 1545 2229
Fragments arranged by size:
2871 2229 1545 1270 97 92
PasI CC'CwG_GG
Cuts at: 1348 4770 1348
Size: 3422 4682
PciI A'CATG_T
Cuts at: 7003 7003
Size: 8104
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2019 2548 4913 1108
Size: 911 529 2365 4299
Fragments arranged by size:
4299 2365 911 529
PmeI GTTT'AAAC
Cuts at: 5408 5408
Size: 8104
PmlI CAC'GTG
Cuts at: 4423 5023 4423
Size: 600 7504
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6173 6205 7686 7718 6173
Size: 32 1481 32 6559
Fragments arranged by size:
6559 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4968 4968
Size: 8104
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8104
PsiI TTA'TAA
Cuts at: 2091 2561 2825 4223 5674 2091
Size: 470 264 1398 1451 4521
Fragments arranged by size:
4521 1451 1398 470 264
PspOMI G'GGCC_C
Cuts at: 4773 4773
Size: 8104
PspXI vC'TCGA_Gb
Cuts at: 4835 4835
Size: 8104
PstI C_TGCA'G
Cuts at: 830 2482 5404 5456 830
Size: 1652 2922 52 3478
Fragments arranged by size:
3478 2922 1652 52
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8104
PvuII CAG'CTG
Cuts at: 3265 4513 5272 5323 3265
Size: 1248 759 51 6046
Fragments arranged by size:
6046 1248 759 51
SacI G_AGCT'C
Cuts at: 4800 4800
Size: 8104
SalI G'TCGA_C
Cuts at: 1936 5370 5446 1936
Size: 3434 76 4594
Fragments arranged by size:
4594 3434 76
SbfI CC_TGCA'GG
Cuts at: 5456 5456
Size: 8104
ScaI AGT'ACT
Cuts at: 6352 8064 6352
Size: 1712 6392
SfcI C'TryA_G
Cuts at: 826 1045 2478 2839 3495 5111 5400 5452 6587
Size: 219 1433 361 656 1616 289 52 1135
Cuts at: 6587 7268 7459 826
Size: 681 191 1471
Fragments arranged by size:
1616 1471 1433 1135 681 656 361 289
219 191 52
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8104
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8104
SmaI CCC'GGG
Cuts at: 1872 5431 1872
Size: 3559 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3963 4756 4835 4842 6208 7109
Size: 197 926 2020 793 79 7 1366 901
Cuts at: 7109 7371 820
Size: 262 1553
Fragments arranged by size:
2020 1553 1366 926 901 793 262 197
79 7
SnaBI TAC'GTA
Cuts at: 493 5204 5895 493
Size: 4711 691 2702
Fragments arranged by size:
4711 2702 691
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8104
SphI G_CATG'C
Cuts at: 1132 5462 1132
Size: 4330 3774
SspI AAT'ATT
Cuts at: 5 52 2794 3392 3730 4921 6028 5
Size: 47 2742 598 338 1191 1107 2081
Fragments arranged by size:
2742 2081 1191 1107 598 338 47
StuI AGG'CCT
Cuts at: 1517 2978 1517
Size: 1461 6643
StyI C'CwwG_G
Cuts at: 513 1065 3562 5543 513
Size: 552 2497 1981 3074
Fragments arranged by size:
3074 2497 1981 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3720 4155 5138 6131 6148 6301 6486
Size: 1255 2140 435 983 993 17 153 185
Cuts at: 6486 6931 325
Size: 445 1498
Fragments arranged by size:
2140 1498 1255 993 983 445 435 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2524 2764 3745
Size: 274 80 33 51 1443 547 240 981
Cuts at: 3745 4270 6350 8062 96
Size: 525 2080 1712 138
Fragments arranged by size:
2080 1712 1443 981 547 525 274 240
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4083 4115 4465 4497 8064 8096 4083
Size: 32 350 32 3567 32 4091
Fragments arranged by size:
4091 3567 350 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8104
XbaI T'CTAG_A
Cuts at: 5440 5440
Size: 8104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4822 4822
Size: 8104
XhoI C'TCGA_G
Cuts at: 1943 4835 1943
Size: 2892 5212
XmaI C'CCGG_G
Cuts at: 1870 5429 1870
Size: 3559 4545
XmnI GAAnn'nnTTC
Cuts at: 6233 6233
Size: 8104
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5912 276
Size: 53 83 186 936 4378 2468
Fragments arranged by size:
4378 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BplI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI
NruI PacI PfoI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI