Restriction map for
pFN21AB9476
(Circular) (Six-base) MAPSORT of: pFN21AB9476.seq Check: 2730 from: 1 to: 5752
pFN21AB9476 5752 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2443 2443
Size: 5752
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 2403 3073 2403
Size: 670 5082
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2315 3815 5199 5467 1632
Size: 431 252 1500 1384 268 1917
Fragments arranged by size:
1917 1500 1384 431 268 252
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 2666 1166
Size: 1500 4252
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AlwNI CAG_nnn'CTG
Cuts at: 2527 3495 5067 2527
Size: 968 1572 3212
Fragments arranged by size:
3212 1572 968
ApaI G_GGCC'C
Cuts at: 2990 2990
Size: 5752
ApaLI G'TGCA_C
Cuts at: 2648 2939 3809 4965 2648
Size: 291 870 1156 3435
Fragments arranged by size:
3435 1156 870 291
ApoI r'AATT_y
Cuts at: 1493 2048 3061 3287 3439 5548 1493
Size: 555 1013 226 152 2109 1697
Fragments arranged by size:
2109 1697 1013 555 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2685 2694 3028 3077 1870
Size: 73 742 9 334 49 4545
Fragments arranged by size:
4545 742 334 73 49 9
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2123 2403 2431 2689 3073
Size: 325 217 627 336 280 28 258 384
Cuts at: 3073 4528 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 627 384 336 325 280 258
217 28
BanII G_rGCy'C
Cuts at: 1391 2367 2466 2661 2990 3071 1391
Size: 976 99 195 329 81 4072
Fragments arranged by size:
4072 976 329 195 99 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2498 928
Size: 971 599 4182
Fragments arranged by size:
4182 971 599
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2318 2567 2994
Size: 107 122 71 631 605 646 249 427
Cuts at: 2994 4363 136
Size: 1369 1525
Fragments arranged by size:
1525 1369 646 631 605 427 249 122
107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2572 2652 2694 2943 2990 3813 4969 1790
Size: 782 80 42 249 47 823 1156 2573
Fragments arranged by size:
2573 1156 823 782 249 80 47 42
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2450 2558 2806 2891 4412 1239
Size: 1211 108 248 85 1521 2579
Fragments arranged by size:
2579 1521 1211 248 108 85
Bpu10I CC'TnA_GC
Cuts at: 2238 2238
Size: 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2567 2774 3877 4742 5040 2567
Size: 207 1103 865 298 3279
Fragments arranged by size:
3279 1103 865 298 207
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2190 3559 3941 4576
Size: 53 83 186 936 657 1369 382 635
Cuts at: 4576 275
Size: 1451
Fragments arranged by size:
1451 1369 936 657 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2831 1186
Size: 1645 4107
BseYI C'CCAG_C
Cuts at: 2119 2148 2193 2399 2562 2673 4955 5451 2119
Size: 29 45 206 163 111 2282 496 2420
Fragments arranged by size:
2420 2282 496 206 163 111 45 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2815 1163
Size: 573 1079 4100
Fragments arranged by size:
4100 1079 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2367 2466 2652 2943 3813 3898 4969 2367
Size: 99 186 291 870 85 1071 3150
Fragments arranged by size:
3150 1071 870 291 186 99 85
BsmI GAATG_Cn'
Cuts at: 2239 2817 3263 3356 2239
Size: 578 446 93 4635
Fragments arranged by size:
4635 578 446 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2367 2466 2572 2652 2661 2694 2943
Size: 399 577 99 106 80 9 33 249
Cuts at: 2943 2990 3071 3813 3898 4969 1391
Size: 47 81 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 577 399 249 106 99
85 81 80 47 33 9
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 2443 3107 844
Size: 1373 226 664 3489
Fragments arranged by size:
3489 1373 664 226
BsrBI CCG'CTC
Cuts at: 1394 1823 2629 3639 1394
Size: 429 806 1010 3507
Fragments arranged by size:
3507 1010 806 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4247 4421 65
Size: 1183 2999 174 1396
Fragments arranged by size:
2999 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4396 4583 1700
Size: 110 2586 187 2869
Fragments arranged by size:
2869 2586 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 2114 3812 4824 2114
Size: 1698 1012 3042
Fragments arranged by size:
3042 1698 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2116 794
Size: 1322 4430
BstBI TT'CG_AA
Cuts at: 2247 4556 2247
Size: 2309 3443
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2644 2668 2644
Size: 24 5728
BstYI r'GATC_y
Cuts at: 1972 2472 3082 3449 3834 3851 5303 5389 5401
Size: 500 610 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 610 500 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2556 2556
Size: 5752
BtgI C'CryG_G
Cuts at: 513 1065 2004 2031 2661 2850 3022 513
Size: 552 939 27 630 189 172 3243
Fragments arranged by size:
3243 939 630 552 189 172 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2913 487
Size: 683 56 1687 3326
Fragments arranged by size:
3326 1687 683 56
BtsI GCAGTG_nn'
Cuts at: 2180 3268 4062 4082 2180
Size: 1088 794 20 3850
Fragments arranged by size:
3850 1088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2310 2347 3199
Size: 54 1425 50 275 18 480 37 852
Cuts at: 3199 4088 5717 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 852 480 275 54 50
43 37 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2031 2261 2372 3682 1353
Size: 678 230 111 1310 3423
Fragments arranged by size:
3423 1310 678 230 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2365 2464 2365
Size: 99 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2315 2450 2558 2806 2891 3815
Size: 393 431 252 135 108 248 85 924
Cuts at: 3815 4412 5199 5467 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 924 787 597 431 393 268 252
248 135 108 85
EcoO109I rG'GnC_Cy
Cuts at: 2533 2986 2533
Size: 453 5299
EcoRI G'AATT_C
Cuts at: 2048 3061 3439 2048
Size: 1013 378 4361
Fragments arranged by size:
4361 1013 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2068 2986 2068
Size: 918 4834
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 3016 4899 1678
Size: 1338 1883 2531
Fragments arranged by size:
2531 1883 1338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 4393 4425 4467
Size: 32 135 32 803 32 1986 32 42
Cuts at: 4467 4499 1373
Size: 32 2626
Fragments arranged by size:
2626 1986 803 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2599 3096 3342 669
Size: 988 281 661 497 246 3079
Fragments arranged by size:
3079 988 661 497 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 2407 3077 2407
Size: 670 5082
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2349 10
Size: 54 1425 343 517 3413
Fragments arranged by size:
3413 1425 517 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2609 2666 2736 3710 4069 4228 518
Size: 648 1443 57 70 974 359 159 2042
Fragments arranged by size:
2042 1443 974 648 359 159 70 57
MspA1I CmG'CkG
Cuts at: 1606 2148 2443 2566 2677 2826 3024 3168 3845
Size: 542 295 123 111 149 198 144 677
Cuts at: 3845 4993 5238 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 542 295 245 198 149
144 123 111
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4261
Fragments arranged by size:
4261 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2990 1348
Size: 1642 4110
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspOMI G'GGCC_C
Cuts at: 2986 2986
Size: 5752
PstI C_TGCA'G
Cuts at: 830 2532 2553 3104 830
Size: 1702 21 551 3478
Fragments arranged by size:
3478 1702 551 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2677 2826 2677
Size: 149 5603
SacI G_AGCT'C
Cuts at: 2367 2466 2367
Size: 99 5653
SacII CC_GC'GG
Cuts at: 3025 3025
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SapI GCTCTTCn'nnn_
Cuts at: 2031 2261 2372 2031
Size: 230 111 5411
Fragments arranged by size:
5411 230 111
SbfI CC_TGCA'GG
Cuts at: 2532 3104 2532
Size: 572 5180
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2528 2549 2760 3100 4235 4916 5107
Size: 219 1483 21 211 340 1135 681 191
Cuts at: 5107 826
Size: 1471
Fragments arranged by size:
1483 1471 1135 681 340 219 211 191
21
SfiI GGCCn_nnn'nGGCC
Cuts at: 2318 2318
Size: 5752
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2687 3030 3079 1872
Size: 815 343 49 4545
Fragments arranged by size:
4545 815 343 49
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2582 2753 3856 4757 5019 820
Size: 197 926 639 171 1103 901 262 1553
Fragments arranged by size:
1553 1103 926 901 639 262 197 171
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2969 1517
Size: 1452 4300
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 2982 3191 513
Size: 552 939 54 924 209 3074
Fragments arranged by size:
3074 939 924 552 209 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2111 2750 3779 3796 3949 4134 4579
Size: 1255 531 639 1029 17 153 185 445
Cuts at: 4579 325
Size: 1498
Fragments arranged by size:
1498 1255 1029 639 531 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2405 2437 5712 5744 2405
Size: 32 3275 32 2413
Fragments arranged by size:
3275 2413 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2145 2145
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2685 3028 3077 1870
Size: 815 343 49 4545
Fragments arranged by size:
4545 815 343 49
XmnI GAAnn'nnTTC
Cuts at: 2048 3881 2048
Size: 1833 3919
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SanDI SexAI
SfoI SrfI SwaI