Restriction map for
pFN21AB9475
(Circular) (Six-base) MAPSORT of: pFN21AB9475.seq Check: 792 from: 1 to: 5734
pFN21AB9475 5734 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3544 278
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3077 1937
Size: 1140 4594
Acc65I G'GTAC_C
Cuts at: 3055 3055
Size: 5734
AclI AA'CG_TT
Cuts at: 1563 3861 4234 1563
Size: 2298 373 3063
Fragments arranged by size:
3063 2298 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2145 2679 2732 2769 2790 2806 3797
Size: 479 34 534 53 37 21 16 991
Cuts at: 3797 5181 5449 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 991 534 479 268 53 37
34 21 16
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5537
AflIII A'CryG_T
Cuts at: 1203 2885 4633 5610 1203
Size: 1682 1748 977 1327
Fragments arranged by size:
1748 1682 1327 977
AgeI A'CCGG_T
Cuts at: 4565 4565
Size: 5734
AhdI GACnn_n'nnGTC
Cuts at: 2800 4463 2800
Size: 1663 4071
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2885 1184
Size: 1701 4033
AlwNI CAG_nnn'CTG
Cuts at: 2080 2710 3477 5049 2080
Size: 630 767 1572 2765
Fragments arranged by size:
2765 1572 767 630
ApaLI G'TGCA_C
Cuts at: 3791 4947 3791
Size: 1156 4578
ApoI r'AATT_y
Cuts at: 1493 3029 3043 3269 3421 5530 1493
Size: 1536 14 226 152 2109 1697
Fragments arranged by size:
2109 1697 1536 226 152 14
AseI AT'TA_AT
Cuts at: 160 1032 4288 160
Size: 872 3256 1606
Fragments arranged by size:
3256 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
AvaI C'yCGr_G
Cuts at: 1870 1943 3059 1870
Size: 73 1116 4545
Fragments arranged by size:
4545 1116 73
BamHI G'GATC_C
Cuts at: 3064 3431 3064
Size: 367 5367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2827 3055 4510 618
Size: 325 217 627 1040 228 1455 1842
Fragments arranged by size:
1842 1455 1040 627 325 228 217
BanII G_rGCy'C
Cuts at: 1391 2087 3053 1391
Size: 696 966 4072
Fragments arranged by size:
4072 966 696
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 2088 2796 928
Size: 971 140 49 708 3866
Fragments arranged by size:
3866 971 708 140 49
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3925 3959 1903
Size: 34 50 34 1904 34 3678
Fragments arranged by size:
3678 1904 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2413 3626 4836 2413
Size: 1213 1210 3311
Fragments arranged by size:
3311 1213 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5734
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4345 136
Size: 107 122 71 631 605 2673 1525
Fragments arranged by size:
2673 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2020 5729 2020
Size: 3709 2025
BlpI GC'TnA_GC
Cuts at: 3151 3151
Size: 5734
Bme1580I G_kGCm'C
Cuts at: 1790 3795 4951 1790
Size: 2005 1156 2573
Fragments arranged by size:
2573 2005 1156
BmgBI CAC'GTC
Cuts at: 1318 2888 1318
Size: 1570 4164
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2046 3465 4423
Size: 416 53 83 399 24 629 1419 958
Cuts at: 4423 442
Size: 1753
Fragments arranged by size:
1753 1419 958 629 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5734
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2152
Size: 5734
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2268 2627 4394 1239
Size: 1029 359 1767 2579
Fragments arranged by size:
2579 1767 1029 359
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3859 4724 5022 3859
Size: 865 298 4571
Fragments arranged by size:
4571 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2228 4397 882
Size: 617 729 2169 2219
Fragments arranged by size:
2219 2169 729 617
BsaAI yAC'GTr
Cuts at: 493 1204 3525 493
Size: 711 2321 2702
Fragments arranged by size:
2702 2321 711
BsaBI GATnn'nnATC
Cuts at: 5728 5728
Size: 5734
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3541 3923 4558 275
Size: 53 83 186 936 2008 382 635 1451
Fragments arranged by size:
2008 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4167 4565 4839 4986 5397 4167
Size: 398 274 147 411 4504
Fragments arranged by size:
4504 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2681 2711 1178
Size: 30 165 30 1278 30 4201
Fragments arranged by size:
4201 1278 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5734
BseYI C'CCAG_C
Cuts at: 2238 4937 5433 2238
Size: 2699 496 2539
Fragments arranged by size:
2699 2539 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5161
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3184 3945 4973 1540
Size: 275 186 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2778 3795 3880 4951 2778
Size: 1017 85 1071 3561
Fragments arranged by size:
3561 1071 1017 85
BsmI GAATG_Cn'
Cuts at: 3245 3338 3245
Size: 93 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2778 3053 3795 3880 4951 1391
Size: 399 297 691 275 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 691 399 297 275 85
BspEI T'CCGG_A
Cuts at: 5397 5397
Size: 5734
BspHI T'CATG_A
Cuts at: 3623 5353 3623
Size: 1730 4004
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2152 3089 844
Size: 1308 937 3489
Fragments arranged by size:
3489 1308 937
BsrBI CCG'CTC
Cuts at: 1394 1823 3621 1394
Size: 429 1798 3507
Fragments arranged by size:
3507 1798 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2473 4229 4403 65
Size: 1183 1225 1756 174 1396
Fragments arranged by size:
1756 1396 1225 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4378 4565 1700
Size: 110 2568 187 2869
Fragments arranged by size:
2869 2568 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5734
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5734
BssSI C'ACGA_G
Cuts at: 3794 4806 3794
Size: 1012 4722
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5734
BstBI TT'CG_AA
Cuts at: 4538 4538
Size: 5734
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5734
BstYI r'GATC_y
Cuts at: 1972 2020 3064 3431 3816 3833 5285 5371 5383
Size: 48 1044 367 385 17 1452 86 12
Cuts at: 5383 5729 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1044 385 367 346 86 48
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4995
Fragments arranged by size:
4995 683 56
BtsI GCAGTG_nn'
Cuts at: 3250 4044 4064 3250
Size: 794 20 4920
Fragments arranged by size:
4920 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5734
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2425 2460 529
Size: 35 1861 35 3803
Fragments arranged by size:
3803 1861 35 35
DraI TTT'AAA
Cuts at: 2962 3038 2962
Size: 76 5658
DraIII CAC_nnn'GTG
Cuts at: 2156 2891 2156
Size: 735 4999
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4741 809
Size: 507 3425 1802
Fragments arranged by size:
3425 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2033 3181 4070
Size: 54 1425 50 275 18 203 1148 889
Cuts at: 4070 5699 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1148 889 275 203 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3181 1537
Size: 275 1369 4090
Fragments arranged by size:
4090 1369 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 2665 2841 3664 1353
Size: 768 544 176 823 3423
Fragments arranged by size:
3423 823 768 544 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4321 4695 4841 387
Size: 1064 138 2732 374 146 1280
Fragments arranged by size:
2732 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2145 2268 2627 2679 2732 2769
Size: 393 479 34 123 359 52 53 37
Cuts at: 2769 2790 2806 3797 4394 5181 5449 1239
Size: 21 16 991 597 787 268 1524
Fragments arranged by size:
1524 991 787 597 479 393 359 268
123 53 52 37 34 21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 2835 2835
Size: 5734
EcoRI G'AATT_C
Cuts at: 3043 3421 3043
Size: 378 5356
EcoRV GAT'ATC
Cuts at: 2299 2299
Size: 5734
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2200 2200
Size: 5734
FspI TGC'GCA
Cuts at: 4240 4240
Size: 5734
HaeII r_GCGC'y
Cuts at: 1678 4881 1678
Size: 3203 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2786 2818 4375 4407 4449
Size: 32 135 32 1214 32 1557 32 42
Cuts at: 4449 4481 1373
Size: 32 2626
Fragments arranged by size:
2626 1557 1214 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3078 3324 669
Size: 988 281 1140 246 3079
Fragments arranged by size:
3079 1140 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2182 748
Size: 1434 4300
HpaI GTT'AAC
Cuts at: 3324 3324
Size: 5734
KpnI G_GTAC'C
Cuts at: 3059 3059
Size: 5734
MfeI C'AATT_G
Cuts at: 3333 3333
Size: 5734
MluI A'CGCG_T
Cuts at: 5610 5610
Size: 5734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2972 4848 5032 1305
Size: 1667 1876 184 2007
Fragments arranged by size:
2007 1876 1667 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2035 10
Size: 54 1425 343 203 3709
Fragments arranged by size:
3709 1425 343 203 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3692 4051 4210 518
Size: 648 2526 359 159 2042
Fragments arranged by size:
2526 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2543 3150 3827 4975 5220 1606
Size: 937 607 677 1148 245 2120
Fragments arranged by size:
2120 1148 937 677 607 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5182
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5734
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5734
NotI GC'GGCC_GC
Cuts at: 3181 3181
Size: 5734
NspI r_CATG'y
Cuts at: 1132 1229 2456 2679 3092 4637 1132
Size: 97 1227 223 413 1545 2229
Fragments arranged by size:
2229 1545 1227 413 223 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5734
PciI A'CATG_T
Cuts at: 4633 4633
Size: 5734
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5734
PmeI GTTT'AAAC
Cuts at: 3038 3038
Size: 5734
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3803 3835 5316 5348 3803
Size: 32 1481 32 4189
Fragments arranged by size:
4189 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2637 1613
Size: 1024 4710
PsiI TTA'TAA
Cuts at: 3304 3304
Size: 5734
PstI C_TGCA'G
Cuts at: 830 2212 2861 3086 830
Size: 1382 649 225 3478
Fragments arranged by size:
3478 1382 649 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5734
PvuII CAG'CTG
Cuts at: 2543 2543
Size: 5734
SalI G'TCGA_C
Cuts at: 1936 3076 1936
Size: 1140 4594
SapI GCTCTTCn'nnn_
Cuts at: 2665 2665
Size: 5734
SbfI CC_TGCA'GG
Cuts at: 3086 3086
Size: 5734
ScaI AGT'ACT
Cuts at: 3982 5694 3982
Size: 1712 4022
SexAI A'CCwGG_T
Cuts at: 2397 2397
Size: 5734
SfcI C'TryA_G
Cuts at: 826 1045 2208 2857 3082 4217 4898 5089 826
Size: 219 1163 649 225 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 681 649 225 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5734
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5734
SmaI CCC'GGG
Cuts at: 1872 3061 1872
Size: 1189 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3838 4739 5001 820
Size: 197 926 1895 901 262 1553
Fragments arranged by size:
1895 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3525 493
Size: 3032 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5734
SphI G_CATG'C
Cuts at: 1132 3092 1132
Size: 1960 3774
SspI AAT'ATT
Cuts at: 5 52 3658 5
Size: 47 3606 2081
Fragments arranged by size:
3606 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5734
StyI C'CwwG_G
Cuts at: 513 1065 2101 3173 513
Size: 552 1036 1072 3074
Fragments arranged by size:
3074 1072 1036 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2449 3761 3778 3931 4116 4561 325
Size: 1255 869 1312 17 153 185 445 1498
Fragments arranged by size:
1498 1312 1255 869 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2460 3980 5692
Size: 274 80 33 51 1443 483 1520 1712
Cuts at: 5692 96
Size: 138
Fragments arranged by size:
1712 1520 1443 483 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2212 2244 2681 2713 5694 5726 2212
Size: 32 437 32 2981 32 2220
Fragments arranged by size:
2981 2220 437 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5734
XbaI T'CTAG_A
Cuts at: 2557 3070 2557
Size: 513 5221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5734
XmaI C'CCGG_G
Cuts at: 1870 3059 1870
Size: 1189 4545
XmnI GAAnn'nnTTC
Cuts at: 3863 3863
Size: 5734
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3542 276
Size: 53 83 186 936 2008 2468
Fragments arranged by size:
2468 2008 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
EcoICRI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI