Restriction map for
pFN21AB9470
(Circular) (Six-base) MAPSORT of: pFN21AB9470.seq Check: 9250 from: 1 to: 6670
pFN21AB9470 6670 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4480 278
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4013 1937
Size: 2076 4594
Acc65I G'GTAC_C
Cuts at: 3991 3991
Size: 6670
AclI AA'CG_TT
Cuts at: 1563 4797 5170 1563
Size: 3234 373 3063
Fragments arranged by size:
3234 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2141 2156 2588 4733 6117 6385 1632
Size: 509 15 432 2145 1384 268 1917
Fragments arranged by size:
2145 1917 1384 509 432 268 15
AfeI AGC'GCT
Cuts at: 2174 2174
Size: 6670
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6473
AflIII A'CryG_T
Cuts at: 1203 5569 6546 1203
Size: 4366 977 1327
Fragments arranged by size:
4366 1327 977
AgeI A'CCGG_T
Cuts at: 2929 5501 2929
Size: 2572 4098
AhdI GACnn_n'nnGTC
Cuts at: 3559 5399 3559
Size: 1840 4830
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3518 1184
Size: 2334 4336
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2438 2470 3254 3286 2438
Size: 32 784 32 5822
Fragments arranged by size:
5822 784 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2797 2884 3148 3459 3679 4413 5985 2797
Size: 87 264 311 220 734 1572 3482
Fragments arranged by size:
3482 1572 734 311 264 220 87
ApaI G_GGCC'C
Cuts at: 2041 2114 2946 3520 3964 2041
Size: 73 832 574 444 4747
Fragments arranged by size:
4747 832 574 444 73
ApaLI G'TGCA_C
Cuts at: 2546 4727 5883 2546
Size: 2181 1156 3333
Fragments arranged by size:
3333 2181 1156
ApoI r'AATT_y
Cuts at: 1493 3979 4205 4357 6466 1493
Size: 2486 226 152 2109 1697
Fragments arranged by size:
2486 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5224 160
Size: 872 4192 1606
Fragments arranged by size:
4192 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
AvaI C'yCGr_G
Cuts at: 1870 1943 2288 2591 3322 3642 3995 1870
Size: 73 345 303 731 320 353 4545
Fragments arranged by size:
4545 731 353 345 320 303 73
AvrII C'CTAG_G
Cuts at: 3925 3925
Size: 6670
BamHI G'GATC_C
Cuts at: 4000 4367 4000
Size: 367 6303
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2389 2926 3226 3602 3953
Size: 325 217 627 602 537 300 376 351
Cuts at: 3953 3991 5446 618
Size: 38 1455 1842
Fragments arranged by size:
1842 1455 627 602 537 376 351 325
300 217 38
BanII G_rGCy'C
Cuts at: 1391 2015 2041 2081 2114 2439 2589 2598 2946
Size: 624 26 40 33 325 150 9 348
Cuts at: 2946 2990 3024 3329 3449 3520 3528 3621 3693
Size: 44 34 305 120 71 8 93 72
Cuts at: 3693 3964 3989 1391
Size: 271 25 4072
Fragments arranged by size:
4072 624 348 325 305 271 150 120
93 72 71 44 40 34 33 26
25 9 8
BbeI G_GCGC'C
Cuts at: 2393 2393
Size: 6670
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5699
BbvCI CC'TCA_GC
Cuts at: 2630 2630
Size: 6670
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2854 2888 3470 3504 4861
Size: 34 50 34 833 34 582 34 1357
Cuts at: 4861 4895 1903
Size: 34 3678
Fragments arranged by size:
3678 1357 833 582 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4562 5772 4562
Size: 1210 5460
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6670
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2036 3404 5281
Size: 107 122 71 631 605 364 1368 1877
Cuts at: 5281 136
Size: 1525
Fragments arranged by size:
1877 1525 1368 631 605 364 122 107
71
BglII A'GATC_T
Cuts at: 6665 6665
Size: 6670
BlpI GC'TnA_GC
Cuts at: 2009 4087 2009
Size: 2078 4592
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2099 2114 2550 2672 2946 3231 3363
Size: 251 58 15 436 122 274 285 132
Cuts at: 3363 3520 3964 4731 5887 1790
Size: 157 444 767 1156 2573
Fragments arranged by size:
2573 1156 767 444 436 285 274 251
157 132 122 58 15
BmgBI CAC'GTC
Cuts at: 1318 2545 1318
Size: 1227 5443
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2840 3147 3523
Size: 416 53 83 399 24 1423 307 376
Cuts at: 3523 4401 5359 442
Size: 878 958 1753
Fragments arranged by size:
1753 1423 958 878 416 399 376 307
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6670
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3329
Size: 6670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2696 3073 3242 3542 5330 1239
Size: 1457 377 169 300 1788 2579
Fragments arranged by size:
2579 1788 1457 377 300 169
Bpu10I CC'TnA_GC
Cuts at: 2630 3692 2630
Size: 1062 5608
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3299 4795 5660 5958 3299
Size: 1496 865 298 4011
Fragments arranged by size:
4011 1496 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3300 5333 882
Size: 617 1801 2033 2219
Fragments arranged by size:
2219 2033 1801 617
BsaAI yAC'GTr
Cuts at: 493 1204 4461 493
Size: 711 3257 2702
Fragments arranged by size:
3257 2702 711
BsaBI GATnn'nnATC
Cuts at: 6664 6664
Size: 6670
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2390 4477 4859 5494
Size: 53 83 186 936 857 2087 382 635
Cuts at: 5494 275
Size: 1451
Fragments arranged by size:
2087 1451 936 857 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2929 5103 5501 5775 5922 6333 2929
Size: 2174 398 274 147 411 3266
Fragments arranged by size:
3266 2174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2438 2468 3311 3341 1178
Size: 30 165 30 1035 30 843 30 4507
Fragments arranged by size:
4507 1035 843 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2161 2459 3008 3229 3745 3892 3923 1186
Size: 975 298 549 221 516 147 31 3933
Fragments arranged by size:
3933 975 549 516 298 221 147 31
BseYI C'CCAG_C
Cuts at: 2107 2640 3389 3539 3620 5873 6369 2107
Size: 533 749 150 81 2253 496 2408
Fragments arranged by size:
2408 2253 749 533 496 150 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3142 3161 3353 3445 3488 1163
Size: 573 1406 19 192 92 43 4345
Fragments arranged by size:
4345 1406 573 192 92 43 19
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3075 4120 4881 5909 1540
Size: 275 186 1074 1045 761 1028 2301
Fragments arranged by size:
2301 1074 1045 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 2589 3087 3693 4731 4816 5887 2550
Size: 39 498 606 1038 85 1071 3333
Fragments arranged by size:
3333 1071 1038 606 498 85 39
BsmI GAATG_Cn'
Cuts at: 4181 4274 4181
Size: 93 6577
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2041 2081 2099 2114 2439 2550
Size: 399 225 26 40 18 15 325 111
Cuts at: 2550 2589 2598 2672 2946 2990 3024 3087 3231
Size: 39 9 74 274 44 34 63 144
Cuts at: 3231 3329 3363 3449 3520 3528 3621 3693 3964
Size: 98 34 86 71 8 93 72 271
Cuts at: 3964 3989 4731 4816 5887 1391
Size: 25 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 325 274 271 225
144 111 98 93 86 85 74 72
71 63 44 40 39 34 34 26
25 18 15 9 8
BspEI T'CCGG_A
Cuts at: 6333 6333
Size: 6670
BspHI T'CATG_A
Cuts at: 2154 4559 6289 2154
Size: 2405 1730 2535
Fragments arranged by size:
2535 2405 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2499 2586 2730 4025 844
Size: 1655 87 144 1295 3489
Fragments arranged by size:
3489 1655 1295 144 87
BsrBI CCG'CTC
Cuts at: 1394 1823 2043 2368 3535 3660 4557 1394
Size: 429 220 325 1167 125 897 3507
Fragments arranged by size:
3507 1167 897 429 325 220 125
BsrDI GCAATG_nn'
Cuts at: 65 1248 2975 5165 5339 65
Size: 1183 1727 2190 174 1396
Fragments arranged by size:
2190 1727 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2929 3117 3309 5314 5501 1700
Size: 110 1119 188 192 2005 187 2869
Fragments arranged by size:
2869 2005 1119 192 188 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6670
BssHII G'CGCG_C
Cuts at: 1925 3653 1925
Size: 1728 4942
BssSI C'ACGA_G
Cuts at: 2363 4730 5742 2363
Size: 2367 1012 3291
Fragments arranged by size:
3291 2367 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3148 3468 3953 794
Size: 2354 320 485 3511
Fragments arranged by size:
3511 2354 485 320
BstBI TT'CG_AA
Cuts at: 5474 5474
Size: 6670
BstEII G'GTnAC_C
Cuts at: 1184 2998 1184
Size: 1814 4856
BstXI CCAn_nnnn'nTGG
Cuts at: 3565 3565
Size: 6670
BstYI r'GATC_y
Cuts at: 1972 2328 4000 4367 4752 4769 6221 6307 6319
Size: 356 1672 367 385 17 1452 86 12
Cuts at: 6319 6665 1972
Size: 346 1977
Fragments arranged by size:
1977 1672 1452 385 367 356 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2706 2706
Size: 6670
BtgI C'CryG_G
Cuts at: 513 1065 2598 3075 3102 513
Size: 552 1533 477 27 4081
Fragments arranged by size:
4081 1533 552 477 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5931
Fragments arranged by size:
5931 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 2982 4186 4980 5000 2414
Size: 568 1204 794 20 4084
Fragments arranged by size:
4084 1204 794 568 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6670
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6635
DraI TTT'AAA
Cuts at: 3974 3974
Size: 6670
DraIII CAC_nnn'GTG
Cuts at: 2554 2554
Size: 6670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5677 809
Size: 507 4361 1802
Fragments arranged by size:
4361 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2828 3072 3396
Size: 54 1425 50 275 18 998 244 324
Cuts at: 3396 4117 5006 6635 8
Size: 721 889 1629 43
Fragments arranged by size:
1629 1425 998 889 721 324 275 244
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3072 4117 1537
Size: 275 1260 1045 4090
Fragments arranged by size:
4090 1260 1045 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2924 3061 4600 1353
Size: 1571 137 1539 3423
Fragments arranged by size:
3423 1571 1539 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2101 5257 5631 5777 387
Size: 1064 138 512 3156 374 146 1280
Fragments arranged by size:
3156 1280 1064 512 374 146 138
EcoICRI GAG'CTC
Cuts at: 2587 3691 2587
Size: 1104 5566
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2141 2156 2588 2696 3073 3242 3542
Size: 393 509 15 432 108 377 169 300
Cuts at: 3542 4733 5330 6117 6385 1239
Size: 1191 597 787 268 1524
Fragments arranged by size:
1524 1191 787 597 509 432 393 377
300 268 169 108 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2611 2705 2611
Size: 94 6576
EcoO109I rG'GnC_Cy
Cuts at: 2037 2111 2293 2488 2842 2942 2962 3051 3183
Size: 74 182 195 354 100 20 89 132
Cuts at: 3183 3417 3517 3553 3960 3961 2037
Size: 234 100 36 407 1 4746
Fragments arranged by size:
4746 407 354 234 195 182 132 100
100 89 74 36 20 1
EcoRI G'AATT_C
Cuts at: 3979 4357 3979
Size: 378 6292
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2326 3184 2326
Size: 858 5812
FspI TGC'GCA
Cuts at: 2974 5176 2974
Size: 2202 4468
HaeII r_GCGC'y
Cuts at: 1678 2176 2344 2393 5817 1678
Size: 498 168 49 3424 2531
Fragments arranged by size:
3424 2531 498 168 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3311 3343 3545 3577 5311
Size: 32 135 32 1739 32 202 32 1734
Cuts at: 5311 5343 5385 5417 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1739 1734 202 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2776 4014 4260 669
Size: 988 281 838 1238 246 3079
Fragments arranged by size:
3079 1238 988 838 281 246
HindIII A'AGCT_T
Cuts at: 748 3931 748
Size: 3183 3487
HpaI GTT'AAC
Cuts at: 4260 4260
Size: 6670
KasI G'GCGC_C
Cuts at: 2389 2389
Size: 6670
KpnI G_GTAC'C
Cuts at: 3995 3995
Size: 6670
MfeI C'AATT_G
Cuts at: 4269 4269
Size: 6670
MluI A'CGCG_T
Cuts at: 6546 6546
Size: 6670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3137 5784 5968 1305
Size: 1832 2647 184 2007
Fragments arranged by size:
2647 2007 1832 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3398 10
Size: 54 1425 343 1566 3282
Fragments arranged by size:
3282 1566 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4628 4987 5146 518
Size: 648 3462 359 159 2042
Fragments arranged by size:
3462 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2634 2644 3459 3715 3889 4086 4763 5911
Size: 1028 10 815 256 174 197 677 1148
Cuts at: 5911 6156 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 1028 815 677 256 245 197
174 10
NarI GG'CG_CC
Cuts at: 2390 2390
Size: 6670
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6118
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6670
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6670
NotI GC'GGCC_GC
Cuts at: 4117 4117
Size: 6670
NspI r_CATG'y
Cuts at: 1132 1229 2464 4028 5573 1132
Size: 97 1235 1564 1545 2229
Fragments arranged by size:
2229 1564 1545 1235 97
PasI CC'CwG_GG
Cuts at: 1348 2081 2236 2297 2324 3128 1348
Size: 733 155 61 27 804 4890
Fragments arranged by size:
4890 804 733 155 61 27
PciI A'CATG_T
Cuts at: 5569 5569
Size: 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2797 3583 1108
Size: 1689 786 4195
Fragments arranged by size:
4195 1689 786
PfoI T'CCnGG_A
Cuts at: 2424 2934 2424
Size: 510 6160
PmeI GTTT'AAAC
Cuts at: 3974 3974
Size: 6670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2470 4739 4771 6252 6284 2438
Size: 32 2269 32 1481 32 2824
Fragments arranged by size:
2824 2269 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2488 2842 3051 3183 3553 2293
Size: 195 354 209 132 370 5410
Fragments arranged by size:
5410 370 354 209 195 132
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6670
PsiI TTA'TAA
Cuts at: 4240 4240
Size: 6670
PspOMI G'GGCC_C
Cuts at: 2037 2110 2942 3516 3960 2037
Size: 73 832 574 444 4747
Fragments arranged by size:
4747 832 574 444 73
PstI C_TGCA'G
Cuts at: 830 2496 4022 830
Size: 1666 1526 3478
Fragments arranged by size:
3478 1666 1526
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6670
PvuII CAG'CTG
Cuts at: 2634 2644 3459 3889 2634
Size: 10 815 430 5415
Fragments arranged by size:
5415 815 430 10
SacI G_AGCT'C
Cuts at: 2589 3693 2589
Size: 1104 5566
SalI G'TCGA_C
Cuts at: 1936 4012 1936
Size: 2076 4594
SapI GCTCTTCn'nnn_
Cuts at: 2924 2924
Size: 6670
SbfI CC_TGCA'GG
Cuts at: 2496 4022 2496
Size: 1526 5144
ScaI AGT'ACT
Cuts at: 4918 6630 4918
Size: 1712 4958
SexAI A'CCwGG_T
Cuts at: 2478 2478
Size: 6670
SfcI C'TryA_G
Cuts at: 826 1045 2492 4018 5153 5834 6025 826
Size: 219 1447 1526 1135 681 191 1471
Fragments arranged by size:
1526 1471 1447 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2036 2036
Size: 6670
SfoI GGC'GCC
Cuts at: 2391 2391
Size: 6670
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6670
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6670
SmaI CCC'GGG
Cuts at: 1872 3644 3997 1872
Size: 1772 353 4545
Fragments arranged by size:
4545 1772 353
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3314 4774 5675 5937 820
Size: 197 926 1371 1460 901 262 1553
Fragments arranged by size:
1553 1460 1371 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4461 493
Size: 3968 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6670
SphI G_CATG'C
Cuts at: 1132 2464 4028 1132
Size: 1332 1564 3774
Fragments arranged by size:
3774 1564 1332
SspI AAT'ATT
Cuts at: 5 52 4594 5
Size: 47 4542 2081
Fragments arranged by size:
4542 2081 47
StuI AGG'CCT
Cuts at: 1517 3668 1517
Size: 2151 4519
StyI C'CwwG_G
Cuts at: 513 1065 2652 3013 3925 4109 513
Size: 552 1587 361 912 184 3074
Fragments arranged by size:
3074 1587 912 552 361 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4697 4714 4867 5052 5497 325
Size: 1255 3117 17 153 185 445 1498
Fragments arranged by size:
3117 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4916 6628 96
Size: 274 80 33 51 1443 2939 1712 138
Fragments arranged by size:
2939 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6630 6662 6630
Size: 32 6638
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6670
XbaI T'CTAG_A
Cuts at: 4006 4006
Size: 6670
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6670
XmaI C'CCGG_G
Cuts at: 1870 3642 3995 1870
Size: 1772 353 4545
Fragments arranged by size:
4545 1772 353
XmnI GAAnn'nnTTC
Cuts at: 4799 4799
Size: 6670
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4478 276
Size: 53 83 186 936 2944 2468
Fragments arranged by size:
2944 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI RsrII SacII SanDI SrfI SwaI XcmI