Restriction map for
pFN21AB9469
(Circular) (Six-base) MAPSORT of: pFN21AB9469.seq Check: 5659 from: 1 to: 7111
pFN21AB9469 7111 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4921 278
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4454 1937
Size: 2517 4594
Acc65I G'GTAC_C
Cuts at: 4432 4432
Size: 7111
AclI AA'CG_TT
Cuts at: 1563 5238 5611 1563
Size: 3675 373 3063
Fragments arranged by size:
3675 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2978 5174 6558 6826 1632
Size: 1346 2196 1384 268 1917
Fragments arranged by size:
2196 1917 1384 1346 268
AfeI AGC'GCT
Cuts at: 2149 3718 3785 2149
Size: 1569 67 5475
Fragments arranged by size:
5475 1569 67
AflII C'TTAA_G
Cuts at: 820 1017 2750 820
Size: 197 1733 5181
Fragments arranged by size:
5181 1733 197
AflIII A'CryG_T
Cuts at: 1203 6010 6987 1203
Size: 4807 977 1327
Fragments arranged by size:
4807 1327 977
AgeI A'CCGG_T
Cuts at: 5942 5942
Size: 7111
AhdI GACnn_n'nnGTC
Cuts at: 3524 5840 3524
Size: 2316 4795
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7111
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7111
AlwNI CAG_nnn'CTG
Cuts at: 2034 2089 2194 3262 4091 4854 6426 2034
Size: 55 105 1068 829 763 1572 2719
Fragments arranged by size:
2719 1572 1068 829 763 105 55
ApaI G_GGCC'C
Cuts at: 2943 3594 4098 2943
Size: 651 504 5956
Fragments arranged by size:
5956 651 504
ApaLI G'TGCA_C
Cuts at: 5168 6324 5168
Size: 1156 5955
ApoI r'AATT_y
Cuts at: 1493 2105 2180 2270 2356 2402 3353 3644 4420
Size: 612 75 90 86 46 951 291 776
Cuts at: 4420 4646 4798 6907 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 951 776 612 291 226 152
90 86 75 46
AseI AT'TA_AT
Cuts at: 160 1032 5665 160
Size: 872 4633 1606
Fragments arranged by size:
4633 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
AvaI C'yCGr_G
Cuts at: 1870 1943 2730 3741 4436 1870
Size: 73 787 1011 695 4545
Fragments arranged by size:
4545 1011 787 695 73
AvrII C'CTAG_G
Cuts at: 3448 3448
Size: 7111
BamHI G'GATC_C
Cuts at: 4441 4808 4441
Size: 367 6744
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4432 5887 618
Size: 325 217 627 2645 1455 1842
Fragments arranged by size:
2645 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2655 2943 3594 3610 3996 4098 4430 1391
Size: 1264 288 651 16 386 102 332 4072
Fragments arranged by size:
4072 1264 651 386 332 288 102 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2451 2984 928
Size: 971 552 533 5055
Fragments arranged by size:
5055 971 552 533
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5302 5336 1903
Size: 34 3365 34 3678
Fragments arranged by size:
3678 3365 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5003 6213 5003
Size: 1210 5901
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7111
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5722 136
Size: 107 122 71 631 605 4050 1525
Fragments arranged by size:
4050 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3690 7106 3690
Size: 3416 3695
BlpI GC'TnA_GC
Cuts at: 2931 4528 2931
Size: 1597 5514
Bme1580I G_kGCm'C
Cuts at: 1790 2943 3594 4098 5172 6328 1790
Size: 1153 651 504 1074 1156 2573
Fragments arranged by size:
2573 1156 1153 1074 651 504
BmgBI CAC'GTC
Cuts at: 1318 4012 1318
Size: 2694 4417
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3331 4220 4842
Size: 416 53 83 399 24 1914 889 622
Cuts at: 4842 5800 442
Size: 958 1753
Fragments arranged by size:
1914 1753 958 889 622 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3430 4149 3430
Size: 719 6392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2111 2707 4179 4419 5771 1239
Size: 803 69 596 1472 240 1352 2579
Fragments arranged by size:
2579 1472 1352 803 596 240 69
Bpu10I CC'TnA_GC
Cuts at: 3609 3609
Size: 7111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2054 4368 5236 6101 6399 2054
Size: 2314 868 865 298 2766
Fragments arranged by size:
2766 2314 868 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 3191 3418 4120 5774 882
Size: 617 1380 312 227 702 1654 2219
Fragments arranged by size:
2219 1654 1380 702 617 312 227
BsaAI yAC'GTr
Cuts at: 493 1204 4902 493
Size: 711 3698 2702
Fragments arranged by size:
3698 2702 711
BsaBI GATnn'nnATC
Cuts at: 3638 7105 3638
Size: 3467 3644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2906 4918 5300 5935
Size: 53 83 186 936 1373 2012 382 635
Cuts at: 5935 275
Size: 1451
Fragments arranged by size:
2012 1451 1373 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2361 5544 5942 6216 6363 6774 2361
Size: 3183 398 274 147 411 2698
Fragments arranged by size:
3183 2698 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2670 2700 1178
Size: 30 165 30 1267 30 5589
Fragments arranged by size:
5589 1267 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3943 1186
Size: 2757 4354
BseYI C'CCAG_C
Cuts at: 6314 6810 6314
Size: 496 6615
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3705 1163
Size: 573 1969 4569
Fragments arranged by size:
4569 1969 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4561 5322 6350 1540
Size: 275 186 2560 761 1028 2301
Fragments arranged by size:
2560 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2655 3543 3610 3681 5172 5257 6328 2655
Size: 888 67 71 1491 85 1071 3438
Fragments arranged by size:
3438 1491 1071 888 85 71 67
BsmI GAATG_Cn'
Cuts at: 3274 4622 4715 3274
Size: 1348 93 5670
Fragments arranged by size:
5670 1348 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2655 2943 3543 3594 3610 3681 3996
Size: 399 865 288 600 51 16 71 315
Cuts at: 3996 4098 4430 5172 5257 6328 1391
Size: 102 332 742 85 1071 2174
Fragments arranged by size:
2174 1071 865 742 600 399 332 315
288 102 85 71 51 16
BspEI T'CCGG_A
Cuts at: 2361 6774 2361
Size: 4413 2698
BspHI T'CATG_A
Cuts at: 3384 5000 6730 3384
Size: 1616 1730 3765
Fragments arranged by size:
3765 1730 1616
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4466 844
Size: 3622 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2392 4998 1394
Size: 429 569 2606 3507
Fragments arranged by size:
3507 2606 569 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4309 5606 5780 65
Size: 1183 3061 1297 174 1396
Fragments arranged by size:
3061 1396 1297 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5755 5942 1700
Size: 110 3945 187 2869
Fragments arranged by size:
3945 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7111
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7111
BssSI C'ACGA_G
Cuts at: 5171 6183 5171
Size: 1012 6099
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7111
BstBI TT'CG_AA
Cuts at: 5915 5915
Size: 7111
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7111
BstYI r'GATC_y
Cuts at: 1972 3690 4240 4441 4808 5193 5210 6662 6748
Size: 1718 550 201 367 385 17 1452 86
Cuts at: 6748 6760 7106 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1718 1452 550 385 367 346 201
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3362 3708 3362
Size: 346 6765
BtgI C'CryG_G
Cuts at: 513 1065 2004 3090 513
Size: 552 939 1086 4534
Fragments arranged by size:
4534 1086 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2236 487
Size: 683 56 1010 5362
Fragments arranged by size:
5362 1010 683 56
BtsI GCAGTG_nn'
Cuts at: 4627 5421 5441 4627
Size: 794 20 6297
Fragments arranged by size:
6297 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7076
DraI TTT'AAA
Cuts at: 4415 4415
Size: 7111
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2197 6118 809
Size: 507 881 3921 1802
Fragments arranged by size:
3921 1802 881 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3624 4558 5447
Size: 54 1425 50 275 18 1794 934 889
Cuts at: 5447 7076 8
Size: 1629 43
Fragments arranged by size:
1794 1629 1425 934 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4558 1537
Size: 275 2746 4090
Fragments arranged by size:
4090 2746 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2730 5041 1353
Size: 1377 2311 3423
Fragments arranged by size:
3423 2311 1377
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3726 4208 5698 6072 6218 387
Size: 1064 138 2137 482 1490 374 146 1280
Fragments arranged by size:
2137 1490 1280 1064 482 374 146 138
EcoICRI GAG'CTC
Cuts at: 2653 3608 2653
Size: 955 6156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2111 2707 2978 4179 4419 5174
Size: 393 410 69 596 271 1201 240 755
Cuts at: 5174 5771 6558 6826 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1201 787 755 597 596 410 393
271 268 240 69
EcoO109I rG'GnC_Cy
Cuts at: 2939 2940 3052 4233 2939
Size: 1 112 1181 5817
Fragments arranged by size:
5817 1181 112 1
EcoRI G'AATT_C
Cuts at: 4420 4798 4420
Size: 378 6733
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3572 4409 3572
Size: 837 6274
FspI TGC'GCA
Cuts at: 5617 5617
Size: 7111
HaeII r_GCGC'y
Cuts at: 1678 2151 3720 3787 6258 1678
Size: 473 1569 67 2471 2531
Fragments arranged by size:
2531 2471 1569 473 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3510 3542 3592 3624 5752
Size: 32 135 32 1938 32 50 32 2128
Cuts at: 5752 5784 5826 5858 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2128 1938 135 50 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4455 4701 669
Size: 988 281 2517 246 3079
Fragments arranged by size:
3079 2517 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7111
HpaI GTT'AAC
Cuts at: 4701 4701
Size: 7111
KpnI G_GTAC'C
Cuts at: 4436 4436
Size: 7111
MfeI C'AATT_G
Cuts at: 4710 4710
Size: 7111
MluI A'CGCG_T
Cuts at: 6987 6987
Size: 7111
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2713 3594 3871 3969 6225 6409 1305
Size: 1408 881 277 98 2256 184 2007
Fragments arranged by size:
2256 2007 1408 881 277 184 98
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5289
Fragments arranged by size:
5289 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3315 3599 3659 5069 5428 5587 518
Size: 648 2149 284 60 1410 359 159 2042
Fragments arranged by size:
2149 2042 1410 648 359 284 159 60
MspA1I CmG'CkG
Cuts at: 1606 2020 4527 5204 6352 6597 1606
Size: 414 2507 677 1148 245 2120
Fragments arranged by size:
2507 2120 1148 677 414 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3090 513
Size: 552 939 1086 4534
Fragments arranged by size:
4534 1086 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7111
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7111
NotI GC'GGCC_GC
Cuts at: 4558 4558
Size: 7111
NspI r_CATG'y
Cuts at: 1132 1229 2784 4469 6014 1132
Size: 97 1555 1685 1545 2229
Fragments arranged by size:
2229 1685 1555 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7111
PciI A'CATG_T
Cuts at: 6010 6010
Size: 7111
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4091 1108
Size: 2983 4128
PfoI T'CCnGG_A
Cuts at: 2559 2791 4195 4337 4364 4396 2559
Size: 232 1404 142 27 32 5274
Fragments arranged by size:
5274 1404 232 142 32 27
PmeI GTTT'AAAC
Cuts at: 4415 4415
Size: 7111
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3028 3060 5180 5212 6693 6725 3028
Size: 32 2120 32 1481 32 3414
Fragments arranged by size:
3414 2120 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7111
PsiI TTA'TAA
Cuts at: 4681 4681
Size: 7111
PspOMI G'GGCC_C
Cuts at: 2939 3590 4094 2939
Size: 651 504 5956
Fragments arranged by size:
5956 651 504
PstI C_TGCA'G
Cuts at: 830 2199 3051 4038 4463 830
Size: 1369 852 987 425 3478
Fragments arranged by size:
3478 1369 987 852 425
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7111
SacI G_AGCT'C
Cuts at: 2655 3610 2655
Size: 955 6156
SalI G'TCGA_C
Cuts at: 1936 4453 1936
Size: 2517 4594
SbfI CC_TGCA'GG
Cuts at: 4463 4463
Size: 7111
ScaI AGT'ACT
Cuts at: 2963 5359 7071 2963
Size: 2396 1712 3003
Fragments arranged by size:
3003 2396 1712
SexAI A'CCwGG_T
Cuts at: 4343 4343
Size: 7111
SfcI C'TryA_G
Cuts at: 826 1045 2195 2498 3047 3391 4034 4459 5594
Size: 219 1150 303 549 344 643 425 1135
Cuts at: 5594 6275 6466 826
Size: 681 191 1471
Fragments arranged by size:
1471 1150 1135 681 643 549 425 344
303 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7111
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7111
SmaI CCC'GGG
Cuts at: 1872 2732 4438 1872
Size: 860 1706 4545
Fragments arranged by size:
4545 1706 860
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2069 2750 4383 5215 6116 6378
Size: 197 926 126 681 1633 832 901 262
Cuts at: 6378 820
Size: 1553
Fragments arranged by size:
1633 1553 926 901 832 681 262 197
126
SnaBI TAC'GTA
Cuts at: 493 4902 493
Size: 4409 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7111
SphI G_CATG'C
Cuts at: 1132 4469 1132
Size: 3337 3774
SspI AAT'ATT
Cuts at: 5 52 2590 3190 3247 5035 5
Size: 47 2538 600 57 1788 2081
Fragments arranged by size:
2538 2081 1788 600 57 47
StuI AGG'CCT
Cuts at: 1517 3970 1517
Size: 2453 4658
StyI C'CwwG_G
Cuts at: 513 1065 2004 2349 2385 2943 3090 3448 4550
Size: 552 939 345 36 558 147 358 1102
Cuts at: 4550 513
Size: 3074
Fragments arranged by size:
3074 1102 939 558 552 358 345 147
36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2845 5138 5155 5308 5493 5938 325
Size: 1255 1265 2293 17 153 185 445 1498
Fragments arranged by size:
2293 1498 1265 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2961 5357 7069
Size: 274 80 33 51 1443 984 2396 1712
Cuts at: 7069 96
Size: 138
Fragments arranged by size:
2396 1712 1443 984 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2649 2681 7071 7103 2649
Size: 32 4390 32 2657
Fragments arranged by size:
4390 2657 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2983 1316
Size: 1667 5444
XbaI T'CTAG_A
Cuts at: 4447 4447
Size: 7111
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2908 2908
Size: 7111
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7111
XmaI C'CCGG_G
Cuts at: 1870 2730 4436 1870
Size: 860 1706 4545
Fragments arranged by size:
4545 1706 860
XmnI GAAnn'nnTTC
Cuts at: 2161 5240 2161
Size: 3079 4032
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4919 276
Size: 53 83 186 936 3385 2468
Fragments arranged by size:
3385 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI BaeI BbeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI