Restriction map for
pFN21AB9466
(Circular) (Six-base) MAPSORT of: pFN21AB9466.seq Check: 7730 from: 1 to: 7036
pFN21AB9466 7036 bp
With 182 enzymes: SgfI *
May 6, 2010 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4846 278
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2399 2555 4379 1937
Size: 462 156 1824 4594
Fragments arranged by size:
4594 1824 462 156
Acc65I G'GTAC_C
Cuts at: 4357 4357
Size: 7036
AclI AA'CG_TT
Cuts at: 1563 3575 5163 5536 1563
Size: 2012 1588 373 3063
Fragments arranged by size:
3063 2012 1588 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2481 3273 3434 3648 3956 4008 4064 5099
Size: 849 792 161 214 308 52 56 1035
Cuts at: 5099 6483 6751 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1035 849 792 308 268 214
161 56 52
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6839
AflIII A'CryG_T
Cuts at: 1203 3696 5935 6912 1203
Size: 2493 2239 977 1327
Fragments arranged by size:
2493 2239 1327 977
AgeI A'CCGG_T
Cuts at: 5867 5867
Size: 7036
AhdI GACnn_n'nnGTC
Cuts at: 5765 5765
Size: 7036
AleI CACnn'nnGTG
Cuts at: 1166 2441 1166
Size: 1275 5761
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3735 1184
Size: 2551 4485
AlwNI CAG_nnn'CTG
Cuts at: 4779 6351 4779
Size: 1572 5464
ApaLI G'TGCA_C
Cuts at: 5093 6249 5093
Size: 1156 5880
ApoI r'AATT_y
Cuts at: 1493 3405 3501 4247 4345 4571 4723 6832 1493
Size: 1912 96 746 98 226 152 2109 1697
Fragments arranged by size:
2109 1912 1697 746 226 152 98 96
AseI AT'TA_AT
Cuts at: 160 1032 3891 5590 160
Size: 872 2859 1699 1606
Fragments arranged by size:
2859 1699 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
AvaI C'yCGr_G
Cuts at: 1870 1943 2344 4253 4361 1870
Size: 73 401 1909 108 4545
Fragments arranged by size:
4545 1909 401 108 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2981 3014 2981
Size: 33 7003
BamHI G'GATC_C
Cuts at: 4366 4733 4366
Size: 367 6669
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2528 3102 4357 5812 618
Size: 325 217 627 741 574 1255 1455 1842
Fragments arranged by size:
1842 1455 1255 741 627 574 325 217
BanII G_rGCy'C
Cuts at: 1391 4355 1391
Size: 2964 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2127 2188 2703 3440 928
Size: 971 228 61 515 737 4524
Fragments arranged by size:
4524 971 737 515 228 61
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5227 5261 1903
Size: 34 3290 34 3678
Fragments arranged by size:
3678 3290 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4928 6138 4928
Size: 1210 5826
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7036
BfrBI ATG'CAT
Cuts at: 4180 4180
Size: 7036
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2526 5647 136
Size: 107 122 71 631 605 854 3121 1525
Fragments arranged by size:
3121 1525 854 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2233 2788 7031 2233
Size: 555 4243 2238
Fragments arranged by size:
4243 2238 555
BlpI GC'TnA_GC
Cuts at: 4453 4453
Size: 7036
Bme1580I G_kGCm'C
Cuts at: 1790 2900 5097 6253 1790
Size: 1110 2197 1156 2573
Fragments arranged by size:
2573 2197 1156 1110
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7036
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2802 2903 4767
Size: 416 53 83 399 24 1385 101 1864
Cuts at: 4767 5725 442
Size: 958 1753
Fragments arranged by size:
1864 1753 1385 958 416 399 101 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7036
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3924 3924
Size: 7036
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2946 3006 3135 4111 5696 1239
Size: 1707 60 129 976 1585 2579
Fragments arranged by size:
2579 1707 1585 976 129 60
Bpu10I CC'TnA_GC
Cuts at: 2064 2064
Size: 7036
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5161 6026 6324 5161
Size: 865 298 5873
Fragments arranged by size:
5873 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3193 5699 882
Size: 617 1694 2506 2219
Fragments arranged by size:
2506 2219 1694 617
BsaAI yAC'GTr
Cuts at: 493 1204 4827 493
Size: 711 3623 2702
Fragments arranged by size:
3623 2702 711
BsaBI GATnn'nnATC
Cuts at: 7030 7030
Size: 7036
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4843 5225 5860 275
Size: 53 83 186 936 3310 382 635 1451
Fragments arranged by size:
3310 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2755 5469 5867 6141 6288 6699 2755
Size: 2714 398 274 147 411 3092
Fragments arranged by size:
3092 2714 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2103 2133 2778 2808 1178
Size: 30 165 30 700 30 645 30 5406
Fragments arranged by size:
5406 700 645 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2121 2124 2673 3207 3220 1186
Size: 935 3 549 534 13 5002
Fragments arranged by size:
5002 935 549 534 13 3
BseYI C'CCAG_C
Cuts at: 2048 3191 3424 6239 6735 2048
Size: 1143 233 2815 496 2349
Fragments arranged by size:
2815 2349 1143 496 233
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2848 1163
Size: 573 1112 5351
Fragments arranged by size:
5351 1112 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4486 5247 6275 1540
Size: 275 186 2485 761 1028 2301
Fragments arranged by size:
2485 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3278 5097 5182 6253 3278
Size: 1819 85 1071 4061
Fragments arranged by size:
4061 1819 1071 85
BsmI GAATG_Cn'
Cuts at: 4547 4640 4547
Size: 93 6943
BsmBI CGTCTCn'nnnn_
Cuts at: 2618 2618
Size: 7036
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2900 3278 4355 5097 5182 6253 1391
Size: 399 1110 378 1077 742 85 1071 2174
Fragments arranged by size:
2174 1110 1077 1071 742 399 378 85
BspEI T'CCGG_A
Cuts at: 6699 6699
Size: 7036
BspHI T'CATG_A
Cuts at: 3681 4925 6655 3681
Size: 1244 1730 4062
Fragments arranged by size:
4062 1730 1244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3870 4391 844
Size: 3026 521 3489
Fragments arranged by size:
3489 3026 521
BsrBI CCG'CTC
Cuts at: 1394 1823 4923 1394
Size: 429 3100 3507
Fragments arranged by size:
3507 3100 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2224 2379 2548 5531 5705 65
Size: 1183 976 155 169 2983 174 1396
Fragments arranged by size:
2983 1396 1183 976 174 169 155
BsrFI r'CCGG_y
Cuts at: 1700 1810 5680 5867 1700
Size: 110 3870 187 2869
Fragments arranged by size:
3870 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7036
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7036
BssSI C'ACGA_G
Cuts at: 5096 6108 5096
Size: 1012 6024
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2438 794
Size: 1644 5392
BstBI TT'CG_AA
Cuts at: 3409 5840 3409
Size: 2431 4605
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7036
BstXI CCAn_nnnn'nTGG
Cuts at: 3113 3113
Size: 7036
BstYI r'GATC_y
Cuts at: 1972 2233 2788 3110 4366 4733 5118 5135 6587
Size: 261 555 322 1256 367 385 17 1452
Cuts at: 6587 6673 6685 7031 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1256 555 385 367 346 322
261 86 17 12
BstZ17I GTA'TAC
Cuts at: 2556 2556
Size: 7036
Bsu36I CC'TnA_GG
Cuts at: 4032 4032
Size: 7036
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5545
Fragments arranged by size:
5545 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6297
Fragments arranged by size:
6297 683 56
BtsI GCAGTG_nn'
Cuts at: 2059 2436 2819 3354 3732 4552 5346 5366 2059
Size: 377 383 535 378 820 794 20 3729
Fragments arranged by size:
3729 820 794 535 383 378 377 20
ClaI AT'CG_AT
Cuts at: 1554 3399 1554
Size: 1845 5191
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7001
DraI TTT'AAA
Cuts at: 4340 4340
Size: 7036
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6043 809
Size: 507 4727 1802
Fragments arranged by size:
4727 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4483 5372 7001
Size: 54 1425 50 275 18 2653 889 1629
Cuts at: 7001 8
Size: 43
Fragments arranged by size:
2653 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4483 1537
Size: 275 2671 4090
Fragments arranged by size:
4090 2671 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3981 4027 4040 4966 1353
Size: 2628 46 13 926 3423
Fragments arranged by size:
3423 2628 926 46 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5623 5997 6143 387
Size: 1064 138 4034 374 146 1280
Fragments arranged by size:
4034 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2481 2946 3006 3135 3273 3434 3648
Size: 393 849 465 60 129 138 161 214
Cuts at: 3648 3956 4008 4064 4111 5099 5696 6483 6751
Size: 308 52 56 47 988 597 787 268
Cuts at: 6751 1239
Size: 1524
Fragments arranged by size:
1524 988 849 787 597 465 393 308
268 214 161 138 129 60 56 52
47
EcoO109I rG'GnC_Cy
Cuts at: 2610 2610
Size: 7036
EcoRI G'AATT_C
Cuts at: 3501 4247 4345 4723 3501
Size: 746 98 378 5814
Fragments arranged by size:
5814 746 378 98
FspI TGC'GCA
Cuts at: 2223 5542 2223
Size: 3319 3717
HaeII r_GCGC'y
Cuts at: 1678 6183 1678
Size: 4505 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3197 3229 5677 5709 5751
Size: 32 135 32 1625 32 2448 32 42
Cuts at: 5751 5783 1373
Size: 32 2626
Fragments arranged by size:
2626 2448 1625 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2400 4380 4626 669
Size: 988 281 462 1980 246 3079
Fragments arranged by size:
3079 1980 988 462 281 246
HindIII A'AGCT_T
Cuts at: 748 2363 2917 3285 748
Size: 1615 554 368 4499
Fragments arranged by size:
4499 1615 554 368
HpaI GTT'AAC
Cuts at: 4626 4626
Size: 7036
KpnI G_GTAC'C
Cuts at: 4361 4361
Size: 7036
MfeI C'AATT_G
Cuts at: 4635 4635
Size: 7036
MluI A'CGCG_T
Cuts at: 6912 6912
Size: 7036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3591 6150 6334 1305
Size: 2286 2559 184 2007
Fragments arranged by size:
2559 2286 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5214
Fragments arranged by size:
5214 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2441 2487 3771 4994 5353 5512 518
Size: 648 1275 46 1284 1223 359 159 2042
Fragments arranged by size:
2042 1284 1275 1223 648 359 159 46
MspA1I CmG'CkG
Cuts at: 1606 4144 4452 5129 6277 6522 1606
Size: 2538 308 677 1148 245 2120
Fragments arranged by size:
2538 2120 1148 677 308 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5545
Fragments arranged by size:
5545 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7036
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7036
NotI GC'GGCC_GC
Cuts at: 4483 4483
Size: 7036
NsiI A_TGCA'T
Cuts at: 4182 4182
Size: 7036
NspI r_CATG'y
Cuts at: 1132 1229 3432 3700 3922 3943 4394 5939 1132
Size: 97 2203 268 222 21 451 1545 2229
Fragments arranged by size:
2229 2203 1545 451 268 222 97 21
PasI CC'CwG_GG
Cuts at: 1348 2870 1348
Size: 1522 5514
PciI A'CATG_T
Cuts at: 3696 5935 3696
Size: 2239 4797
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7036
PfoI T'CCnGG_A
Cuts at: 2923 3800 2923
Size: 877 6159
PmeI GTTT'AAAC
Cuts at: 4340 4340
Size: 7036
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5105 5137 6618 6650 5105
Size: 32 1481 32 5491
Fragments arranged by size:
5491 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2610 2610
Size: 7036
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7036
PsiI TTA'TAA
Cuts at: 4606 4606
Size: 7036
PstI C_TGCA'G
Cuts at: 830 3795 4388 830
Size: 2965 593 3478
Fragments arranged by size:
3478 2965 593
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7036
SalI G'TCGA_C
Cuts at: 1936 2398 4378 1936
Size: 462 1980 4594
Fragments arranged by size:
4594 1980 462
SanDI GG'GwC_CC
Cuts at: 2610 2610
Size: 7036
SapI GCTCTTCn'nnn_
Cuts at: 4040 4040
Size: 7036
SbfI CC_TGCA'GG
Cuts at: 4388 4388
Size: 7036
ScaI AGT'ACT
Cuts at: 3797 5284 6996 3797
Size: 1487 1712 3837
Fragments arranged by size:
3837 1712 1487
SexAI A'CCwGG_T
Cuts at: 3748 3748
Size: 7036
SfcI C'TryA_G
Cuts at: 826 1045 3516 3791 4384 5519 6200 6391 826
Size: 219 2471 275 593 1135 681 191 1471
Fragments arranged by size:
2471 1471 1135 681 593 275 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7036
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7036
SmaI CCC'GGG
Cuts at: 1872 4363 1872
Size: 2491 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5140 6041 6303 820
Size: 197 926 3197 901 262 1553
Fragments arranged by size:
3197 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4827 493
Size: 4334 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7036
SphI G_CATG'C
Cuts at: 1132 3432 4394 1132
Size: 2300 962 3774
Fragments arranged by size:
3774 2300 962
SspI AAT'ATT
Cuts at: 5 52 4295 4960 5
Size: 47 4243 665 2081
Fragments arranged by size:
4243 2081 665 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7036
StyI C'CwwG_G
Cuts at: 513 1065 2004 2639 3106 4475 513
Size: 552 939 635 467 1369 3074
Fragments arranged by size:
3074 1369 939 635 552 467
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5063 5080 5233 5418 5863 325
Size: 1255 3483 17 153 185 445 1498
Fragments arranged by size:
3483 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3795 5282 6994
Size: 274 80 33 51 1443 1818 1487 1712
Cuts at: 6994 96
Size: 138
Fragments arranged by size:
1818 1712 1487 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2103 2135 6996 7028 2103
Size: 32 4861 32 2111
Fragments arranged by size:
4861 2111 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7036
XbaI T'CTAG_A
Cuts at: 4372 4372
Size: 7036
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3265 3669 3265
Size: 404 6632
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7036
XmaI C'CCGG_G
Cuts at: 1870 4361 1870
Size: 2491 4545
XmnI GAAnn'nnTTC
Cuts at: 5165 5165
Size: 7036
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4844 276
Size: 53 83 186 936 3310 2468
Fragments arranged by size:
3310 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI
SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BsiWI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI
SrfI SwaI