Restriction map for
pFN21AB9464
(Circular) (Six-base) MAPSORT of: pFN21AB9464.seq Check: 6296 from: 1 to: 8275
pFN21AB9464 8275 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3265 6085 278
Size: 53 83 186 936 1729 2820 2468
Fragments arranged by size:
2820 2468 1729 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3906 5618 1937
Size: 1969 1712 4594
Fragments arranged by size:
4594 1969 1712
Acc65I G'GTAC_C
Cuts at: 5596 5596
Size: 8275
AclI AA'CG_TT
Cuts at: 1563 6402 6775 1563
Size: 4839 373 3063
Fragments arranged by size:
4839 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 3329 3525 3692 4683 5174 6338 7722
Size: 737 960 196 167 991 491 1164 1384
Cuts at: 7722 7990 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1164 991 960 737 491 268
196 167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8078
AflIII A'CryG_T
Cuts at: 1203 3084 4550 7174 8151 1203
Size: 1881 1466 2624 977 1327
Fragments arranged by size:
2624 1881 1466 1327 977
AgeI A'CCGG_T
Cuts at: 7106 7106
Size: 8275
AhdI GACnn_n'nnGTC
Cuts at: 4202 4463 4705 7004 4202
Size: 261 242 2299 5473
Fragments arranged by size:
5473 2299 261 242
AleI CACnn'nnGTG
Cuts at: 1166 4319 1166
Size: 3153 5122
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5238 1184
Size: 4054 4221
AlwNI CAG_nnn'CTG
Cuts at: 3670 3871 4304 5390 5512 6018 7590 3670
Size: 201 433 1086 122 506 1572 4355
Fragments arranged by size:
4355 1572 1086 506 433 201 122
ApaI G_GGCC'C
Cuts at: 2557 2824 2919 2557
Size: 267 95 7913
Fragments arranged by size:
7913 267 95
ApaLI G'TGCA_C
Cuts at: 4554 6332 7488 4554
Size: 1778 1156 5341
Fragments arranged by size:
5341 1778 1156
ApoI r'AATT_y
Cuts at: 1493 4585 5584 5810 5962 8071 1493
Size: 3092 999 226 152 2109 1697
Fragments arranged by size:
3092 2109 1697 999 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6829 160
Size: 872 5797 1606
Fragments arranged by size:
5797 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8275
AvaI C'yCGr_G
Cuts at: 1870 1943 2215 2640 2700 2841 2894 3478 5600
Size: 73 272 425 60 141 53 584 2122
Cuts at: 5600 1870
Size: 4545
Fragments arranged by size:
4545 2122 584 425 272 141 73 60
53
AvrII C'CTAG_G
Cuts at: 2900 2900
Size: 8275
BamHI G'GATC_C
Cuts at: 5605 5972 5605
Size: 367 7908
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2273 4793 5596 7051 618
Size: 325 217 627 486 2520 803 1455 1842
Fragments arranged by size:
2520 1842 1455 803 627 486 325 217
BanII G_rGCy'C
Cuts at: 1391 2557 2824 2888 2919 4918 5304 5594 1391
Size: 1166 267 64 31 1999 386 290 4072
Fragments arranged by size:
4072 1999 1166 386 290 267 64 31
BbeI G_GCGC'C
Cuts at: 4797 4797
Size: 8275
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3886 4122 4704 4779 928
Size: 971 1987 236 582 75 4424
Fragments arranged by size:
4424 1987 971 582 236 75
BbvCI CC'TCA_GC
Cuts at: 2576 2576
Size: 8275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3577 3611 6466 6500 1903
Size: 34 50 34 1556 34 2855 34 3678
Fragments arranged by size:
3678 2855 1556 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5452 6167 7377 5452
Size: 715 1210 6350
Fragments arranged by size:
6350 1210 715
BclI T'GATC_A
Cuts at: 1548 4525 4687 1548
Size: 2977 162 5136
Fragments arranged by size:
5136 2977 162
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2905 6886 136
Size: 107 122 71 631 605 1233 3981 1525
Fragments arranged by size:
3981 1525 1233 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2982 8270 2982
Size: 5288 2987
BlpI GC'TnA_GC
Cuts at: 5692 5692
Size: 8275
Bme1580I G_kGCm'C
Cuts at: 1790 2557 2824 2919 4558 6336 7492 1790
Size: 767 267 95 1639 1778 1156 2573
Fragments arranged by size:
2573 1778 1639 1156 767 267 95
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8275
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4411 6006 6964
Size: 416 53 83 399 24 2994 1595 958
Cuts at: 6964 442
Size: 1753
Fragments arranged by size:
2994 1753 1595 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4938 1056
Size: 3882 4393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2627 2687 3234 4081 4338 4438 5288 6935
Size: 1388 60 547 847 257 100 850 1647
Cuts at: 6935 1239
Size: 2579
Fragments arranged by size:
2579 1647 1388 850 847 547 257 100
60
Bpu10I CC'TnA_GC
Cuts at: 2576 5424 2576
Size: 2848 5427
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3041 3280 5163 6400 7265 7563 3041
Size: 239 1883 1237 865 298 3753
Fragments arranged by size:
3753 1883 1237 865 298 239
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2013 2637 2969 3413 3654 4189 4663
Size: 617 514 624 332 444 241 535 474
Cuts at: 4663 6938 882
Size: 2275 2219
Fragments arranged by size:
2275 2219 624 617 535 514 474 444
332 241
BsaAI yAC'GTr
Cuts at: 493 1204 6066 493
Size: 711 4862 2702
Fragments arranged by size:
4862 2702 711
BsaBI GATnn'nnATC
Cuts at: 8269 8269
Size: 8275
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2539 3262 4794 6082
Size: 53 83 186 936 1006 723 1532 1288
Cuts at: 6082 6464 7099 275
Size: 382 635 1451
Fragments arranged by size:
1532 1451 1288 1006 936 723 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2150 2457 2586 3573 4326 6708 7106 7380 7527
Size: 307 129 987 753 2382 398 274 147
Cuts at: 7527 7938 2150
Size: 411 2487
Fragments arranged by size:
2487 2382 987 753 411 398 307 274
147 129
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3358 3388 3549 3579 4054
Size: 30 165 30 1955 30 161 30 475
Cuts at: 4054 4084 4436 4466 4635 4665 5483 5513 1178
Size: 30 352 30 169 30 818 30 3940
Fragments arranged by size:
3940 1955 818 475 352 169 165 161
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2031 2491 3269 3450 4844 4849 4895 4939
Size: 845 460 778 181 1394 5 46 44
Cuts at: 4939 5103 5166 5204 5225 5504 5507 5510 1186
Size: 164 63 38 21 279 3 3 3951
Fragments arranged by size:
3951 1394 845 778 460 279 181 164
63 46 44 38 21 5 3 3
BseYI C'CCAG_C
Cuts at: 2514 2888 3454 3928 4040 4153 4898 4969 5400
Size: 374 566 474 112 113 745 71 431
Cuts at: 5400 7478 7974 2514
Size: 2078 496 2815
Fragments arranged by size:
2815 2078 745 566 496 474 431 374
113 112 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4892 1163
Size: 573 3156 4546
Fragments arranged by size:
4546 3156 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2731 2912 5725 6486 7514 1540
Size: 275 186 730 181 2813 761 1028 2301
Fragments arranged by size:
2813 2301 1028 761 730 275 186 181
BsiHKAI G_wGCw'C
Cuts at: 2214 2871 4558 4918 5304 6336 6421 7492 2214
Size: 657 1687 360 386 1032 85 1071 2997
Fragments arranged by size:
2997 1687 1071 1032 657 386 360 85
BsmI GAATG_Cn'
Cuts at: 3615 4071 4973 5298 5323 5786 5879 3615
Size: 456 902 325 25 463 93 6011
Fragments arranged by size:
6011 902 463 456 325 93 25
BsmBI CGTCTCn'nnnn_
Cuts at: 3253 3253
Size: 8275
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2214 2557 2824 2871 2888 2919 4558
Size: 399 424 343 267 47 17 31 1639
Cuts at: 4558 4918 5304 5594 6336 6421 7492 1391
Size: 360 386 290 742 85 1071 2174
Fragments arranged by size:
2174 1639 1071 742 424 399 386 360
343 290 267 85 47 31 17
BspEI T'CCGG_A
Cuts at: 2150 2457 7938 2150
Size: 307 5481 2487
Fragments arranged by size:
5481 2487 307
BspHI T'CATG_A
Cuts at: 6164 7894 6164
Size: 1730 6545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5630 844
Size: 4786 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2551 2569 2611 2671 2791 2838 4857
Size: 429 728 18 42 60 120 47 2019
Cuts at: 4857 6162 1394
Size: 1305 3507
Fragments arranged by size:
3507 2019 1305 728 429 120 60 47
42 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 3595 4239 6770 6944 65
Size: 1183 2347 644 2531 174 1396
Fragments arranged by size:
2531 2347 1396 1183 644 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2481 4867 6919 7106 1700
Size: 110 198 473 2386 2052 187 2869
Fragments arranged by size:
2869 2386 2052 473 198 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8275
BssHII G'CGCG_C
Cuts at: 1925 2633 1925
Size: 708 7567
BssSI C'ACGA_G
Cuts at: 4877 4911 5407 6335 7347 4877
Size: 34 496 928 1012 5805
Fragments arranged by size:
5805 1012 928 496 34
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5318 794
Size: 4524 3751
BstBI TT'CG_AA
Cuts at: 7079 7079
Size: 8275
BstEII G'GTnAC_C
Cuts at: 1184 3754 1184
Size: 2570 5705
BstXI CCAn_nnnn'nTGG
Cuts at: 2690 4082 4321 2690
Size: 1392 239 6644
Fragments arranged by size:
6644 1392 239
BstYI r'GATC_y
Cuts at: 1972 2982 4301 5605 5972 6357 6374 7826 7912
Size: 1010 1319 1304 367 385 17 1452 86
Cuts at: 7912 7924 8270 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1319 1304 1010 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4100 4100
Size: 8275
BtgI C'CryG_G
Cuts at: 513 1065 2023 2280 2494 2593 2653 2725 4722
Size: 552 958 257 214 99 60 72 1997
Cuts at: 4722 513
Size: 4066
Fragments arranged by size:
4066 1997 958 552 257 214 99 72
60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7536
Fragments arranged by size:
7536 683 56
BtsI GCAGTG_nn'
Cuts at: 2151 5212 5485 5791 6585 6605 2151
Size: 3061 273 306 794 20 3821
Fragments arranged by size:
3821 3061 794 306 273 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8240
DraI TTT'AAA
Cuts at: 5579 5579
Size: 8275
DraIII CAC_nnn'GTG
Cuts at: 4419 4419
Size: 8275
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2258 7282 809
Size: 507 942 5024 1802
Fragments arranged by size:
5024 1802 942 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2376 2596 2656
Size: 54 1425 50 275 18 546 220 60
Cuts at: 2656 2728 2909 4072 5542 5722 6611 8240 8
Size: 72 181 1163 1470 180 889 1629 43
Fragments arranged by size:
1629 1470 1425 1163 889 546 275 220
181 180 72 60 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2728 2909 5722 1537
Size: 275 916 181 2813 4090
Fragments arranged by size:
4090 2813 916 275 181
EarI CTCTTCn'nnn_
Cuts at: 1353 2073 2949 6205 1353
Size: 720 876 3256 3423
Fragments arranged by size:
3423 3256 876 720
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3650 6862 7236 7382 387
Size: 1064 138 2061 3212 374 146 1280
Fragments arranged by size:
3212 2061 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4916 5302 4916
Size: 386 7889
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2369 2627 2687 3234 3329 3525 3692
Size: 393 737 258 60 547 95 196 167
Cuts at: 3692 4081 4338 4438 4683 5174 5288 6338 6935
Size: 389 257 100 245 491 114 1050 597
Cuts at: 6935 7722 7990 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1050 787 737 597 547 491 393
389 268 258 257 245 196 167 114
100 95 60
EcoNI CCTnn'n_nnAGG
Cuts at: 2114 2405 2114
Size: 291 7984
EcoO109I rG'GnC_Cy
Cuts at: 2358 2572 2614 2674 2820 2846 2915 2926 3627
Size: 214 42 60 146 26 69 11 701
Cuts at: 3627 3646 2358
Size: 19 6987
Fragments arranged by size:
6987 701 214 146 69 60 42 26
19 11
EcoRI G'AATT_C
Cuts at: 5584 5962 5584
Size: 378 7897
EcoRV GAT'ATC
Cuts at: 5353 5353
Size: 8275
FspI TGC'GCA
Cuts at: 6781 6781
Size: 8275
HaeII r_GCGC'y
Cuts at: 1678 4797 7422 1678
Size: 3119 2625 2531
Fragments arranged by size:
3119 2625 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2822 2854 3071 3103 3185
Size: 32 135 32 1250 32 217 32 82
Cuts at: 3185 3217 3250 3282 3358 3390 3876 3908 4093
Size: 32 33 32 76 32 486 32 185
Cuts at: 4093 4125 4188 4220 4286 4318 4449 4481 4672
Size: 32 63 32 66 32 131 32 191
Cuts at: 4672 4691 4704 4723 6916 6948 6990 7022 1373
Size: 19 13 19 2193 32 42 32 2626
Fragments arranged by size:
2626 2193 1250 486 217 191 185 135
131 82 76 66 63 42 33 32
32 32 32 32 32 32 32 32
32 32 32 32 32 19 19 13
HincII GTy'rAC
Cuts at: 669 1657 1938 5619 5865 669
Size: 988 281 3681 246 3079
Fragments arranged by size:
3681 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4538 748
Size: 3790 4485
HpaI GTT'AAC
Cuts at: 5865 5865
Size: 8275
KasI G'GCGC_C
Cuts at: 4793 4793
Size: 8275
KpnI G_GTAC'C
Cuts at: 5600 5600
Size: 8275
MfeI C'AATT_G
Cuts at: 2193 4428 5874 2193
Size: 2235 1446 4594
Fragments arranged by size:
4594 2235 1446
MluI A'CGCG_T
Cuts at: 8151 8151
Size: 8275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3945 4517 4524 7389 7573 1305
Size: 2640 572 7 2865 184 2007
Fragments arranged by size:
2865 2640 2007 572 184 7
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4074 5544 10
Size: 54 1425 343 2242 1470 2741
Fragments arranged by size:
2741 2242 1470 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2279 4319 6233 6592 6751 518
Size: 648 843 270 2040 1914 359 159 2042
Fragments arranged by size:
2042 2040 1914 843 648 359 270 159
MspA1I CmG'CkG
Cuts at: 1606 2025 2514 2727 4127 4837 5512 5691 6368
Size: 419 489 213 1400 710 675 179 677
Cuts at: 6368 7516 7761 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1400 1148 710 677 675 489 419
245 213 179
NaeI GCC'GGC
Cuts at: 2483 2483
Size: 8275
NarI GG'CG_CC
Cuts at: 4794 4794
Size: 8275
NcoI C'CATG_G
Cuts at: 513 1065 2280 2494 4722 513
Size: 552 1215 214 2228 4066
Fragments arranged by size:
4066 2228 1215 552 214
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8275
NgoMIV G'CCGG_C
Cuts at: 2481 2481
Size: 8275
NheI G'CTAG_C
Cuts at: 1052 4934 1052
Size: 3882 4393
NotI GC'GGCC_GC
Cuts at: 5722 5722
Size: 8275
NspI r_CATG'y
Cuts at: 1132 1229 3088 4370 4708 5633 7178 1132
Size: 97 1859 1282 338 925 1545 2229
Fragments arranged by size:
2229 1859 1545 1282 925 338 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8275
PciI A'CATG_T
Cuts at: 3084 7174 3084
Size: 4090 4185
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5390 1108
Size: 4282 3993
PfoI T'CCnGG_A
Cuts at: 3353 5304 3353
Size: 1951 6324
PmeI GTTT'AAAC
Cuts at: 5579 5579
Size: 8275
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6344 6376 7857 7889 6344
Size: 32 1481 32 6730
Fragments arranged by size:
6730 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2572 2846 2926 3627 2572
Size: 274 80 701 7220
Fragments arranged by size:
7220 701 274 80
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8275
PsiI TTA'TAA
Cuts at: 3830 5845 3830
Size: 2015 6260
PspOMI G'GGCC_C
Cuts at: 2553 2820 2915 2553
Size: 267 95 7913
Fragments arranged by size:
7913 267 95
PstI C_TGCA'G
Cuts at: 830 5627 830
Size: 4797 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8275
PvuII CAG'CTG
Cuts at: 2514 4127 5512 2514
Size: 1613 1385 5277
Fragments arranged by size:
5277 1613 1385
SacI G_AGCT'C
Cuts at: 4918 5304 4918
Size: 386 7889
SacII CC_GC'GG
Cuts at: 2026 2728 2026
Size: 702 7573
SalI G'TCGA_C
Cuts at: 1936 5617 1936
Size: 3681 4594
SanDI GG'GwC_CC
Cuts at: 2846 2846
Size: 8275
SapI GCTCTTCn'nnn_
Cuts at: 2073 2073
Size: 8275
SbfI CC_TGCA'GG
Cuts at: 5627 5627
Size: 8275
ScaI AGT'ACT
Cuts at: 6523 8235 6523
Size: 1712 6563
SfcI C'TryA_G
Cuts at: 826 1045 2467 2797 3134 5623 6758 7439 7630
Size: 219 1422 330 337 2489 1135 681 191
Cuts at: 7630 826
Size: 1471
Fragments arranged by size:
2489 1471 1422 1135 681 337 330 219
191
SfoI GGC'GCC
Cuts at: 4795 4795
Size: 8275
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8275
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8275
SmaI CCC'GGG
Cuts at: 1872 2217 2702 5602 1872
Size: 345 485 2900 4545
Fragments arranged by size:
4545 2900 485 345
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3056 3295 5178 6379 7280 7542
Size: 197 926 1113 239 1883 1201 901 262
Cuts at: 7542 820
Size: 1553
Fragments arranged by size:
1883 1553 1201 1113 926 901 262 239
197
SnaBI TAC'GTA
Cuts at: 493 6066 493
Size: 5573 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8275
SphI G_CATG'C
Cuts at: 1132 4370 5633 1132
Size: 3238 1263 3774
Fragments arranged by size:
3774 3238 1263
SspI AAT'ATT
Cuts at: 5 52 5256 6199 5
Size: 47 5204 943 2081
Fragments arranged by size:
5204 2081 943 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8275
StyI C'CwwG_G
Cuts at: 513 1065 2280 2494 2900 4722 4992 5714 513
Size: 552 1215 214 406 1822 270 722 3074
Fragments arranged by size:
3074 1822 1215 722 552 406 270 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3350 3385 4430 6302 6319 6472 6657
Size: 1255 1770 35 1045 1872 17 153 185
Cuts at: 6657 7102 325
Size: 445 1498
Fragments arranged by size:
1872 1770 1498 1255 1045 445 185 153
35 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2097 2808 6521
Size: 274 80 33 51 1443 120 711 3713
Cuts at: 6521 8233 96
Size: 1712 138
Fragments arranged by size:
3713 1712 1443 711 274 138 120 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2130 2162 3549 3581 4054 4086 5396 5428 8235
Size: 32 1387 32 473 32 1310 32 2807
Cuts at: 8235 8267 2130
Size: 32 2138
Fragments arranged by size:
2807 2138 1387 1310 473 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8275
XbaI T'CTAG_A
Cuts at: 5611 5611
Size: 8275
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2177 2398 5236 2177
Size: 221 2838 5216
Fragments arranged by size:
5216 2838 221
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8275
XmaI C'CCGG_G
Cuts at: 1870 2215 2700 5600 1870
Size: 345 485 2900 4545
Fragments arranged by size:
4545 2900 485 345
XmnI GAAnn'nnTTC
Cuts at: 6404 6404
Size: 8275
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3263 6083 276
Size: 53 83 186 936 1729 2820 2468
Fragments arranged by size:
2820 2468 1729 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BplI BsiWI
BstZ17I FalI FseI FspAI NruI NsiI PacI PmlI
PspXI PsrI RsrII SexAI SfiI SrfI SwaI