Restriction map for
pFN21AB9462
(Circular) (Six-base) MAPSORT of: pFN21AB9462.seq Check: 2836 from: 1 to: 7441
pFN21AB9462 7441 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2336 3507 3965 2336
Size: 1171 458 5812
Fragments arranged by size:
5812 1171 458
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5251 278
Size: 53 83 186 936 3715 2468
Fragments arranged by size:
3715 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4530 4784 1937
Size: 2593 254 4594
Fragments arranged by size:
4594 2593 254
Acc65I G'GTAC_C
Cuts at: 3645 4762 3645
Size: 1117 6324
AclI AA'CG_TT
Cuts at: 1563 5568 5941 1563
Size: 4005 373 3063
Fragments arranged by size:
4005 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2457 4099 5504 6888 7156 1632
Size: 825 1642 1405 1384 268 1917
Fragments arranged by size:
1917 1642 1405 1384 825 268
AfeI AGC'GCT
Cuts at: 4061 4695 4061
Size: 634 6807
AflII C'TTAA_G
Cuts at: 820 1017 2213 3344 820
Size: 197 1196 1131 4917
Fragments arranged by size:
4917 1196 1131 197
AflIII A'CryG_T
Cuts at: 1203 2449 2610 3680 6340 7317 1203
Size: 1246 161 1070 2660 977 1327
Fragments arranged by size:
2660 1327 1246 1070 977 161
AgeI A'CCGG_T
Cuts at: 6272 6272
Size: 7441
AhdI GACnn_n'nnGTC
Cuts at: 6170 6170
Size: 7441
AleI CACnn'nnGTG
Cuts at: 1166 3830 1166
Size: 2664 4777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3356 1184
Size: 2172 5269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 7409
AlwNI CAG_nnn'CTG
Cuts at: 2362 3547 4138 5184 6756 2362
Size: 1185 591 1046 1572 3047
Fragments arranged by size:
3047 1572 1185 1046 591
ApaI G_GGCC'C
Cuts at: 2041 2041
Size: 7441
ApaLI G'TGCA_C
Cuts at: 3166 4303 5498 6654 3166
Size: 1137 1195 1156 3953
Fragments arranged by size:
3953 1195 1156 1137
ApoI r'AATT_y
Cuts at: 1493 2125 4750 4976 5128 7237 1493
Size: 632 2625 226 152 2109 1697
Fragments arranged by size:
2625 2109 1697 632 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5995 160
Size: 872 4963 1606
Fragments arranged by size:
4963 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
AvaI C'yCGr_G
Cuts at: 1870 1943 2351 3865 4337 4766 1870
Size: 73 408 1514 472 429 4545
Fragments arranged by size:
4545 1514 472 429 408 73
BamHI G'GATC_C
Cuts at: 4771 5138 4771
Size: 367 7074
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2309 2599 3332 3645 3953
Size: 325 217 627 522 290 733 313 308
Cuts at: 3953 4424 4762 6217 618
Size: 471 338 1455 1842
Fragments arranged by size:
1842 1455 733 627 522 471 338 325
313 308 290 217
BanII G_rGCy'C
Cuts at: 1391 2041 2189 2483 2784 2936 2951 3306 3758
Size: 650 148 294 301 152 15 355 452
Cuts at: 3758 3945 4671 4760 1391
Size: 187 726 89 4072
Fragments arranged by size:
4072 726 650 452 355 301 294 187
152 148 89 15
BbeI G_GCGC'C
Cuts at: 2313 2313
Size: 7441
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3602 928
Size: 971 1703 4767
Fragments arranged by size:
4767 1703 971
BbvCI CC'TCA_GC
Cuts at: 2063 2988 2063
Size: 925 6516
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5632 5666 1903
Size: 34 50 34 3611 34 3678
Fragments arranged by size:
3678 3611 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5333 6543 5333
Size: 1210 6231
BclI T'GATC_A
Cuts at: 1548 3822 1548
Size: 2274 5167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2073 4275 6052
Size: 107 122 71 631 605 401 2202 1777
Cuts at: 6052 136
Size: 1525
Fragments arranged by size:
2202 1777 1525 631 605 401 122 107
71
BglII A'GATC_T
Cuts at: 7436 7436
Size: 7441
BlpI GC'TnA_GC
Cuts at: 3125 3939 4858 3125
Size: 814 919 5708
Fragments arranged by size:
5708 919 814
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2604 2650 3170 4075 4307 4338 4566
Size: 251 563 46 520 905 232 31 228
Cuts at: 4566 5502 6658 1790
Size: 936 1156 2573
Fragments arranged by size:
2573 1156 936 905 563 520 251 232
228 46 31
BmgBI CAC'GTC
Cuts at: 1318 3157 3681 1318
Size: 1839 524 5078
Fragments arranged by size:
5078 1839 524
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2165 2752 5172
Size: 416 53 83 399 24 748 587 2420
Cuts at: 5172 6130 442
Size: 958 1753
Fragments arranged by size:
2420 1753 958 748 587 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7441
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3772 3772
Size: 7441
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2261 2317 2843 2906 2996 3034 6101 1239
Size: 1022 56 526 63 90 38 3067 2579
Fragments arranged by size:
3067 2579 1022 526 90 63 56 38
Bpu10I CC'TnA_GC
Cuts at: 2063 2988 2063
Size: 925 6516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2636 3125 3767 4220 4703 5566 6431 6729 2636
Size: 489 642 453 483 863 865 298 3348
Fragments arranged by size:
3348 865 863 642 489 483 453 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2491 4334 4480 6104 882
Size: 617 992 1843 146 1624 2219
Fragments arranged by size:
2219 1843 1624 992 617 146
BsaAI yAC'GTr
Cuts at: 493 1204 2613 3886 4302 5232 493
Size: 711 1409 1273 416 930 2702
Fragments arranged by size:
2702 1409 1273 930 711 416
BsaBI GATnn'nnATC
Cuts at: 3995 7435 3995
Size: 3440 4001
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2310 5248 5630 6265
Size: 53 83 186 936 777 2938 382 635
Cuts at: 6265 275
Size: 1451
Fragments arranged by size:
2938 1451 936 777 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5874 6272 6546 6693 7104 5874
Size: 398 274 147 411 6211
Fragments arranged by size:
6211 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2573 2603 1178
Size: 30 165 30 912 30 228 30 6016
Fragments arranged by size:
6016 912 228 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2051 2993 1186
Size: 865 942 5634
Fragments arranged by size:
5634 942 865
BseYI C'CCAG_C
Cuts at: 2305 2943 3336 4138 4273 4690 6644 7140 2305
Size: 638 393 802 135 417 1954 496 2606
Fragments arranged by size:
2606 1954 802 638 496 417 393 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 2954 3242 3248 3485 1163
Size: 573 657 561 288 6 237 5119
Fragments arranged by size:
5119 657 573 561 288 237 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 3295 4440 4891 5652 6680
Size: 275 186 1039 255 1145 451 761 1028
Cuts at: 6680 1540
Size: 2301
Fragments arranged by size:
2301 1145 1039 1028 761 451 275 255
186
BsiHKAI G_wGCw'C
Cuts at: 2483 3170 4236 4307 4671 5502 5587 6658 2483
Size: 687 1066 71 364 831 85 1071 3266
Fragments arranged by size:
3266 1071 1066 831 687 364 85 71
BsiWI C'GTAC_G
Cuts at: 3025 3025
Size: 7441
BsmI GAATG_Cn'
Cuts at: 2316 4515 4952 5045 2316
Size: 2199 437 93 4712
Fragments arranged by size:
4712 2199 437 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2189 2483 2604 2650 2784 2936
Size: 399 251 148 294 121 46 134 152
Cuts at: 2936 2951 3170 3306 3758 3945 4075 4236 4307
Size: 15 219 136 452 187 130 161 71
Cuts at: 4307 4338 4566 4671 4760 5502 5587 6658 1391
Size: 31 228 105 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 452 399 294 251 228
219 187 161 152 148 136 134 130
121 105 89 85 71 46 31 15
BspEI T'CCGG_A
Cuts at: 7104 7104
Size: 7441
BspHI T'CATG_A
Cuts at: 3930 5330 7060 3930
Size: 1400 1730 4311
Fragments arranged by size:
4311 1730 1400
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2295 2336 3507 3965 4796 844
Size: 1451 41 1171 458 831 3489
Fragments arranged by size:
3489 1451 1171 831 458 41
BsrBI CCG'CTC
Cuts at: 1394 1823 2703 3517 3537 3555 3589 5328 1394
Size: 429 880 814 20 18 34 1739 3507
Fragments arranged by size:
3507 1739 880 814 429 34 20 18
BsrDI GCAATG_nn'
Cuts at: 65 1248 5936 6110 65
Size: 1183 4688 174 1396
Fragments arranged by size:
4688 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 3072 3723 6085 6272 1700
Size: 110 425 837 651 2362 187 2869
Fragments arranged by size:
2869 2362 837 651 425 187 110
BsrGI T'GTAC_A
Cuts at: 96 4377 4449 96
Size: 4281 72 3088
Fragments arranged by size:
4281 3088 72
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7441
BssSI C'ACGA_G
Cuts at: 5501 6513 5501
Size: 1012 6429
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3271 3547 3615 3720 4561 794
Size: 2477 276 68 105 841 3674
Fragments arranged by size:
3674 2477 841 276 105 68
BstBI TT'CG_AA
Cuts at: 2831 6245 2831
Size: 3414 4027
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7441
BstXI CCAn_nnnn'nTGG
Cuts at: 2497 2497
Size: 7441
BstYI r'GATC_y
Cuts at: 1972 4771 5138 5523 5540 6992 7078 7090 7436
Size: 2799 367 385 17 1452 86 12 346
Cuts at: 7436 1972
Size: 1977
Fragments arranged by size:
2799 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2628 2774 3249 3789 513
Size: 552 1563 146 475 540 4165
Fragments arranged by size:
4165 1563 552 540 475 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2737 3361 3784 487
Size: 683 56 1511 624 423 4144
Fragments arranged by size:
4144 1511 683 624 423 56
BtsI GCAGTG_nn'
Cuts at: 2422 3115 3625 4957 5751 5771 2422
Size: 693 510 1332 794 20 4092
Fragments arranged by size:
4092 1332 794 693 510 20
ClaI AT'CG_AT
Cuts at: 1554 3234 1554
Size: 1680 5761
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2185 2220 529
Size: 35 1621 35 5750
Fragments arranged by size:
5750 1621 35 35
DraI TTT'AAA
Cuts at: 4745 4745
Size: 7441
DraIII CAC_nnn'GTG
Cuts at: 4180 4180
Size: 7441
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6448 809
Size: 507 5132 1802
Fragments arranged by size:
5132 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2179 2499 3292
Size: 54 1425 50 275 18 349 320 793
Cuts at: 3292 3527 3786 4104 4437 4602 4698 4888 5777
Size: 235 259 318 333 165 96 190 889
Cuts at: 5777 7406 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 793 349 333 320 318
275 259 235 190 165 96 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 4437 4888 1537
Size: 275 1480 1145 451 4090
Fragments arranged by size:
4090 1480 1145 451 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2009 2522 3785 4547 5371 1353
Size: 656 513 1263 762 824 3423
Fragments arranged by size:
3423 1263 824 762 656 513
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2801 6028 6402 6548 387
Size: 1064 138 1212 3227 374 146 1280
Fragments arranged by size:
3227 1280 1212 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2481 4669 2481
Size: 2188 5253
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2261 2317 2457 2843 2906 2996 3034
Size: 393 629 56 140 386 63 90 38
Cuts at: 3034 4099 5504 6101 6888 7156 1239
Size: 1065 1405 597 787 268 1524
Fragments arranged by size:
1524 1405 1065 787 629 597 393 386
268 140 90 63 56 38
EcoNI CCTnn'n_nnAGG
Cuts at: 2332 2978 3143 3639 2332
Size: 646 165 496 6134
Fragments arranged by size:
6134 646 496 165
EcoO109I rG'GnC_Cy
Cuts at: 4687 4687
Size: 7441
EcoRI G'AATT_C
Cuts at: 2125 4750 5128 2125
Size: 2625 378 4438
Fragments arranged by size:
4438 2625 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2038 4654 2038
Size: 2616 4825
FspI TGC'GCA
Cuts at: 3452 5947 3452
Size: 2495 4946
HaeII r_GCGC'y
Cuts at: 1678 2313 2381 2555 3427 4063 4494 4697 6588
Size: 635 68 174 872 636 431 203 1891
Cuts at: 6588 1678
Size: 2531
Fragments arranged by size:
2531 1891 872 636 635 431 203 174
68
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2573 2605 4646
Size: 32 135 32 743 32 226 32 2041
Cuts at: 4646 4678 6082 6114 6156 6188 1373
Size: 32 1404 32 42 32 2626
Fragments arranged by size:
2626 2041 1404 743 226 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3364 4785 5031 669
Size: 988 281 1426 1421 246 3079
Fragments arranged by size:
3079 1426 1421 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3743 748
Size: 2995 4446
HpaI GTT'AAC
Cuts at: 5031 5031
Size: 7441
KasI G'GCGC_C
Cuts at: 2309 2309
Size: 7441
KpnI G_GTAC'C
Cuts at: 3649 4766 3649
Size: 1117 6324
MfeI C'AATT_G
Cuts at: 5040 5040
Size: 7441
MluI A'CGCG_T
Cuts at: 7317 7317
Size: 7441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4638 6555 6739 1305
Size: 3333 1917 184 2007
Fragments arranged by size:
3333 2007 1917 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2501 4106 4700 10
Size: 54 1425 343 669 1605 594 2751
Fragments arranged by size:
2751 1605 1425 669 594 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 3830 4148 5399 5758 5917 518
Size: 648 1329 1335 318 1251 359 159 2042
Fragments arranged by size:
2042 1335 1329 1251 648 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 2409 2943 3340 3448 3547 4102 4135 4201
Size: 803 534 397 108 99 555 33 66
Cuts at: 4201 4857 5534 6682 6927 1606
Size: 656 677 1148 245 2120
Fragments arranged by size:
2120 1148 803 677 656 555 534 397
245 108 99 66 33
NaeI GCC'GGC
Cuts at: 3725 3725
Size: 7441
NarI GG'CG_CC
Cuts at: 2310 2310
Size: 7441
NcoI C'CATG_G
Cuts at: 513 1065 2628 3249 513
Size: 552 1563 621 4705
Fragments arranged by size:
4705 1563 621 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7441
NgoMIV G'CCGG_C
Cuts at: 3723 3723
Size: 7441
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7441
NotI GC'GGCC_GC
Cuts at: 4888 4888
Size: 7441
NspI r_CATG'y
Cuts at: 1132 1229 3613 3697 4288 4522 4799 6344 1132
Size: 97 2384 84 591 234 277 1545 2229
Fragments arranged by size:
2384 2229 1545 591 277 234 97 84
PasI CC'CwG_GG
Cuts at: 1348 2095 3762 4504 1348
Size: 747 1667 742 4285
Fragments arranged by size:
4285 1667 747 742
PciI A'CATG_T
Cuts at: 6340 6340
Size: 7441
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2239 2629 4180 1108
Size: 1131 390 1551 4369
Fragments arranged by size:
4369 1551 1131 390
PfoI T'CCnGG_A
Cuts at: 2820 3485 4191 2820
Size: 665 706 6070
Fragments arranged by size:
6070 706 665
PmeI GTTT'AAAC
Cuts at: 4745 4745
Size: 7441
PmlI CAC'GTG
Cuts at: 4302 4302
Size: 7441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5510 5542 7023 7055 5510
Size: 32 1481 32 5896
Fragments arranged by size:
5896 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3285 4715 1613
Size: 1672 1430 4339
Fragments arranged by size:
4339 1672 1430
PsiI TTA'TAA
Cuts at: 5011 5011
Size: 7441
PspOMI G'GGCC_C
Cuts at: 2037 2037
Size: 7441
PspXI vC'TCGA_Gb
Cuts at: 2351 2351
Size: 7441
PstI C_TGCA'G
Cuts at: 830 3566 4134 4793 830
Size: 2736 568 659 3478
Fragments arranged by size:
3478 2736 659 568
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7441
PvuII CAG'CTG
Cuts at: 2943 3340 3448 3547 4102 4135 2943
Size: 397 108 99 555 33 6249
Fragments arranged by size:
6249 555 397 108 99 33
SacI G_AGCT'C
Cuts at: 2483 4671 2483
Size: 2188 5253
SalI G'TCGA_C
Cuts at: 1936 4783 1936
Size: 2847 4594
SbfI CC_TGCA'GG
Cuts at: 4793 4793
Size: 7441
ScaI AGT'ACT
Cuts at: 5689 7401 5689
Size: 1712 5729
SexAI A'CCwGG_T
Cuts at: 3654 3654
Size: 7441
SfcI C'TryA_G
Cuts at: 826 1045 3324 3562 4130 4279 4789 5924 6605
Size: 219 2279 238 568 149 510 1135 681
Cuts at: 6605 6796 826
Size: 191 1471
Fragments arranged by size:
2279 1471 1135 681 568 510 238 219
191 149
SfoI GGC'GCC
Cuts at: 2311 2311
Size: 7441
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7441
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7441
SmaI CCC'GGG
Cuts at: 1872 4768 1872
Size: 2896 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2213 2351 2651 3140 3344 3746
Size: 197 926 270 138 300 489 204 402
Cuts at: 3746 4235 4682 5545 6446 6708 820
Size: 489 447 863 901 262 1553
Fragments arranged by size:
1553 926 901 863 489 489 447 402
300 270 262 204 197 138
SnaBI TAC'GTA
Cuts at: 493 5232 493
Size: 4739 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7441
SphI G_CATG'C
Cuts at: 1132 3613 4288 4799 1132
Size: 2481 675 511 3774
Fragments arranged by size:
3774 2481 675 511
SspI AAT'ATT
Cuts at: 5 52 5365 5
Size: 47 5313 2081
Fragments arranged by size:
5313 2081 47
StuI AGG'CCT
Cuts at: 1517 2163 2714 3863 1517
Size: 646 551 1149 5095
Fragments arranged by size:
5095 1149 646 551
StyI C'CwwG_G
Cuts at: 513 1065 2628 2784 3249 3949 4880 513
Size: 552 1563 156 465 700 931 3074
Fragments arranged by size:
3074 1563 931 700 552 465 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2357 2443 2447 2473 3350 4054 5468
Size: 1255 777 86 4 26 877 704 1414
Cuts at: 5468 5485 5638 5823 6268 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1414 1255 877 777 704 445 185
153 86 26 17 4
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3348 3468 4377
Size: 274 80 33 51 1443 1371 120 909
Cuts at: 4377 4449 5687 7399 96
Size: 72 1238 1712 138
Fragments arranged by size:
1712 1443 1371 1238 909 274 138 120
80 72 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 7401 7433 2111
Size: 32 5258 32 2119
Fragments arranged by size:
5258 2119 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2639 1316
Size: 1323 6118
XbaI T'CTAG_A
Cuts at: 4777 4777
Size: 7441
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3058 3814 3058
Size: 756 6685
XhoI C'TCGA_G
Cuts at: 1943 2351 1943
Size: 408 7033
XmaI C'CCGG_G
Cuts at: 1870 4766 1870
Size: 2896 4545
XmnI GAAnn'nnTTC
Cuts at: 5570 5570
Size: 7441
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5249 276
Size: 53 83 186 936 3715 2468
Fragments arranged by size:
3715 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsmBI BstZ17I Bsu36I EcoRV
FseI FspAI NruI NsiI PacI PpuMI PsrI RsrII
SacII SanDI SapI SfiI SrfI SwaI