Restriction map for
pFN21AB9461
(Circular) (Six-base) MAPSORT of: pFN21AB9461.seq Check: 6367 from: 1 to: 6379
pFN21AB9461 6379 bp
With 182 enzymes: SgfI *
February 27, 2009 14:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2975 3186 2975
Size: 211 6168
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4189 278
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3722 1937
Size: 1785 4594
Acc65I G'GTAC_C
Cuts at: 3700 3700
Size: 6379
AclI AA'CG_TT
Cuts at: 1563 4506 4879 1563
Size: 2943 373 3063
Fragments arranged by size:
3063 2943 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2549 2642 4442 5826 6094 1632
Size: 917 93 1800 1384 268 1917
Fragments arranged by size:
1917 1800 1384 917 268 93
AflII C'TTAA_G
Cuts at: 820 1017 3185 820
Size: 197 2168 4014
Fragments arranged by size:
4014 2168 197
AflIII A'CryG_T
Cuts at: 1203 2274 5278 6255 1203
Size: 1071 3004 977 1327
Fragments arranged by size:
3004 1327 1071 977
AgeI A'CCGG_T
Cuts at: 5210 5210
Size: 6379
AhdI GACnn_n'nnGTC
Cuts at: 5108 5108
Size: 6379
AleI CACnn'nnGTG
Cuts at: 1166 3380 1166
Size: 2214 4165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6379
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3276 3308 3276
Size: 32 6347
AlwNI CAG_nnn'CTG
Cuts at: 2638 4122 5694 2638
Size: 1484 1572 3323
Fragments arranged by size:
3323 1572 1484
ApaLI G'TGCA_C
Cuts at: 3469 4436 5592 3469
Size: 967 1156 4256
Fragments arranged by size:
4256 1156 967
ApoI r'AATT_y
Cuts at: 1493 2370 2675 2705 2879 3239 3688 3914 4066
Size: 877 305 30 174 360 449 226 152
Cuts at: 4066 6175 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 877 449 360 305 226 174
152 30
AseI AT'TA_AT
Cuts at: 160 1032 4933 160
Size: 872 3901 1606
Fragments arranged by size:
3901 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
AvaI C'yCGr_G
Cuts at: 1870 1943 2924 3704 1870
Size: 73 981 780 4545
Fragments arranged by size:
4545 981 780 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3599 3632 3599
Size: 33 6346
BamHI G'GATC_C
Cuts at: 2169 3709 4076 2169
Size: 1540 367 4472
Fragments arranged by size:
4472 1540 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2699 3700 5155 618
Size: 325 217 627 912 1001 1455 1842
Fragments arranged by size:
1842 1455 1001 912 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2072 3698 1391
Size: 681 1626 4072
Fragments arranged by size:
4072 1626 681
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5408
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2681 2715 4570 4604 1903
Size: 34 744 34 1855 34 3678
Fragments arranged by size:
3678 1855 744 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2434 4271 5481 2434
Size: 1837 1210 3332
Fragments arranged by size:
3332 1837 1210
BclI T'GATC_A
Cuts at: 1548 2511 1548
Size: 963 5416
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4990 136
Size: 107 122 71 631 605 3318 1525
Fragments arranged by size:
3318 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3006 6374 3006
Size: 3368 3011
BlpI GC'TnA_GC
Cuts at: 3796 3796
Size: 6379
Bme1580I G_kGCm'C
Cuts at: 1790 3473 4440 5596 1790
Size: 1683 967 1156 2573
Fragments arranged by size:
2573 1683 1156 967
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6379
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4110 5068 442
Size: 416 53 83 399 24 2693 958 1753
Fragments arranged by size:
2693 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3429 1056
Size: 2373 4006
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2244 2558 2680 3516 5039 1239
Size: 1005 314 122 836 1523 2579
Fragments arranged by size:
2579 1523 1005 836 314 122
Bpu10I CC'TnA_GC
Cuts at: 2501 2501
Size: 6379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 4504 5369 5667 2708
Size: 1796 865 298 3420
Fragments arranged by size:
3420 1796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2354 2734 5042 882
Size: 617 855 380 2308 2219
Fragments arranged by size:
2308 2219 855 617 380
BsaAI yAC'GTr
Cuts at: 493 1204 4170 493
Size: 711 2966 2702
Fragments arranged by size:
2966 2702 711
BsaBI GATnn'nnATC
Cuts at: 6373 6373
Size: 6379
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4186 4568 5203 275
Size: 53 83 186 936 2653 382 635 1451
Fragments arranged by size:
2653 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3486 4812 5210 5484 5631 6042 3486
Size: 1326 398 274 147 411 3823
Fragments arranged by size:
3823 1326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6154
Fragments arranged by size:
6154 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2096 1186
Size: 907 3 5469
Fragments arranged by size:
5469 907 3
BseYI C'CCAG_C
Cuts at: 3346 3511 5582 6078 3346
Size: 165 2071 496 3647
Fragments arranged by size:
3647 2071 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3612 1163
Size: 573 1876 3930
Fragments arranged by size:
3930 1876 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3829 4590 5618 1540
Size: 275 186 1828 761 1028 2301
Fragments arranged by size:
2301 1828 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2072 3473 4440 4525 5596 2072
Size: 1401 967 85 1071 2855
Fragments arranged by size:
2855 1401 1071 967 85
BsmI GAATG_Cn'
Cuts at: 3890 3983 3890
Size: 93 6286
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2072 3473 3698 4440 4525 5596 1391
Size: 399 282 1401 225 742 85 1071 2174
Fragments arranged by size:
2174 1401 1071 742 399 282 225 85
BspEI T'CCGG_A
Cuts at: 6042 6042
Size: 6379
BspHI T'CATG_A
Cuts at: 4268 5998 4268
Size: 1730 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 3186 3264 3734 844
Size: 2131 211 78 470 3489
Fragments arranged by size:
3489 2131 470 211 78
BsrBI CCG'CTC
Cuts at: 1394 1823 4266 1394
Size: 429 2443 3507
Fragments arranged by size:
3507 2443 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2610 4874 5048 65
Size: 1183 1362 2264 174 1396
Fragments arranged by size:
2264 1396 1362 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3246 5023 5210 1700
Size: 110 1436 1777 187 2869
Fragments arranged by size:
2869 1777 1436 187 110
BsrGI T'GTAC_A
Cuts at: 96 2277 3576 96
Size: 2181 1299 2899
Fragments arranged by size:
2899 2181 1299
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6379
BssSI C'ACGA_G
Cuts at: 4439 5451 4439
Size: 1012 5367
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6379
BstBI TT'CG_AA
Cuts at: 5183 5183
Size: 6379
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6379
BstXI CCAn_nnnn'nTGG
Cuts at: 2710 2710
Size: 6379
BstYI r'GATC_y
Cuts at: 1972 2169 2348 3006 3709 4076 4461 4478 5930
Size: 197 179 658 703 367 385 17 1452
Cuts at: 5930 6016 6028 6374 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 703 658 385 367 346 197
179 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3081 513
Size: 552 939 1077 3811
Fragments arranged by size:
3811 1077 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5640
Fragments arranged by size:
5640 683 56
BtsI GCAGTG_nn'
Cuts at: 2433 3895 4689 4709 2433
Size: 1462 794 20 4103
Fragments arranged by size:
4103 1462 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2476 2511 3340 3375 529
Size: 35 1912 35 829 35 3533
Fragments arranged by size:
3533 1912 829 35 35 35
DraI TTT'AAA
Cuts at: 3683 3683
Size: 6379
DraIII CAC_nnn'GTG
Cuts at: 3468 3468
Size: 6379
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5386 809
Size: 507 4070 1802
Fragments arranged by size:
4070 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3826 4715
Size: 54 1425 50 275 18 177 1819 889
Cuts at: 4715 6344 8
Size: 1629 43
Fragments arranged by size:
1819 1629 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3826 1537
Size: 275 2014 4090
Fragments arranged by size:
4090 2014 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2070 3114 4309 1353
Size: 717 1044 1195 3423
Fragments arranged by size:
3423 1195 1044 717
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4966 5340 5486 387
Size: 1064 138 3377 374 146 1280
Fragments arranged by size:
3377 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2070 2070
Size: 6379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2244 2549 2558 2642 2680 3516 4442
Size: 393 612 305 9 84 38 836 926
Cuts at: 4442 5039 5826 6094 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 926 836 787 612 597 393 305
268 84 38 9
EcoRI G'AATT_C
Cuts at: 2370 2879 3688 4066 2370
Size: 509 809 378 4683
Fragments arranged by size:
4683 809 509 378
FspI TGC'GCA
Cuts at: 4885 4885
Size: 6379
HaeII r_GCGC'y
Cuts at: 1678 5526 1678
Size: 3848 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2428 2450 2460 2482 5020
Size: 32 135 32 856 22 10 22 2538
Cuts at: 5020 5052 5094 5126 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2538 856 135 42 32 32 32
32 22 22 10
HincII GTy'rAC
Cuts at: 669 1657 1938 2469 3723 3969 669
Size: 988 281 531 1254 246 3079
Fragments arranged by size:
3079 1254 988 531 281 246
HindIII A'AGCT_T
Cuts at: 748 3581 3624 748
Size: 2833 43 3503
Fragments arranged by size:
3503 2833 43
HpaI GTT'AAC
Cuts at: 3969 3969
Size: 6379
KpnI G_GTAC'C
Cuts at: 3704 3704
Size: 6379
MfeI C'AATT_G
Cuts at: 3978 3978
Size: 6379
MluI A'CGCG_T
Cuts at: 6255 6255
Size: 6379
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2284 3246 3624 5493 5677 1305
Size: 979 962 378 1869 184 2007
Fragments arranged by size:
2007 1869 979 962 378 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4380
Fragments arranged by size:
4380 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2062 3380 4337 4696 4855 518
Size: 648 896 1318 957 359 159 2042
Fragments arranged by size:
2042 1318 957 896 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3795 4472 5620 5865 1606
Size: 2189 677 1148 245 2120
Fragments arranged by size:
2189 2120 1148 677 245
NaeI GCC'GGC
Cuts at: 3248 3248
Size: 6379
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4888
Fragments arranged by size:
4888 939 552
NdeI CA'TA_TG
Cuts at: 387 2059 2683 3379 387
Size: 1672 624 696 3387
Fragments arranged by size:
3387 1672 696 624
NgoMIV G'CCGG_C
Cuts at: 3246 3246
Size: 6379
NheI G'CTAG_C
Cuts at: 1052 3425 1052
Size: 2373 4006
NotI GC'GGCC_GC
Cuts at: 3826 3826
Size: 6379
NspI r_CATG'y
Cuts at: 1132 1229 2278 3737 5282 1132
Size: 97 1049 1459 1545 2229
Fragments arranged by size:
2229 1545 1459 1049 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6379
PciI A'CATG_T
Cuts at: 2274 5278 2274
Size: 3004 3375
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2327 1108
Size: 1219 5160
PfoI T'CCnGG_A
Cuts at: 2550 2550
Size: 6379
PmeI GTTT'AAAC
Cuts at: 3683 3683
Size: 6379
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4448 4480 5961 5993 4448
Size: 32 1481 32 4834
Fragments arranged by size:
4834 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6379
PsiI TTA'TAA
Cuts at: 3949 3949
Size: 6379
PstI C_TGCA'G
Cuts at: 830 3261 3541 3731 830
Size: 2431 280 190 3478
Fragments arranged by size:
3478 2431 280 190
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6379
SacI G_AGCT'C
Cuts at: 2072 2072
Size: 6379
SalI G'TCGA_C
Cuts at: 1936 3721 1936
Size: 1785 4594
SbfI CC_TGCA'GG
Cuts at: 3731 3731
Size: 6379
ScaI AGT'ACT
Cuts at: 2987 4627 6339 2987
Size: 1640 1712 3027
Fragments arranged by size:
3027 1712 1640
SexAI A'CCwGG_T
Cuts at: 3528 3528
Size: 6379
SfcI C'TryA_G
Cuts at: 826 1045 2753 3257 3537 3727 4862 5543 5734
Size: 219 1708 504 280 190 1135 681 191
Cuts at: 5734 826
Size: 1471
Fragments arranged by size:
1708 1471 1135 681 504 280 219 191
190
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6379
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6379
SmaI CCC'GGG
Cuts at: 1872 3706 1872
Size: 1834 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 3185 4483 5384 5646 820
Size: 197 926 780 462 1298 901 262 1553
Fragments arranged by size:
1553 1298 926 901 780 462 262 197
SnaBI TAC'GTA
Cuts at: 493 4170 493
Size: 3677 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6379
SphI G_CATG'C
Cuts at: 1132 3737 1132
Size: 2605 3774
SspI AAT'ATT
Cuts at: 5 52 4303 5
Size: 47 4251 2081
Fragments arranged by size:
4251 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6379
StyI C'CwwG_G
Cuts at: 513 1065 2004 2909 3093 3818 513
Size: 552 939 905 184 725 3074
Fragments arranged by size:
3074 939 905 725 552 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3218 4406 4423 4576 4761 5206 325
Size: 1255 1638 1188 17 153 185 445 1498
Fragments arranged by size:
1638 1498 1255 1188 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2277 2985 3560
Size: 274 80 33 51 1443 300 708 575
Cuts at: 3560 3576 4625 6337 96
Size: 16 1049 1712 138
Fragments arranged by size:
1712 1443 1049 708 575 300 274 138
80 51 33 16
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 6339 6371 2762
Size: 32 3545 32 2770
Fragments arranged by size:
3545 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6379
XbaI T'CTAG_A
Cuts at: 3715 3715
Size: 6379
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6379
XmaI C'CCGG_G
Cuts at: 1870 3704 1870
Size: 1834 4545
XmnI GAAnn'nnTTC
Cuts at: 3206 4508 3206
Size: 1302 5077
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4187 276
Size: 53 83 186 936 2653 2468
Fragments arranged by size:
2653 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI