Restriction map for
pFN21AB9449
(Circular) (Six-base) MAPSORT of: pFN21AB9449.seq Check: 2784 from: 1 to: 6100
pFN21AB9449 6100 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3910 278
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3443 1937
Size: 1506 4594
Acc65I G'GTAC_C
Cuts at: 3421 3421
Size: 6100
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3296 4163 5547 5815 1632
Size: 1664 867 1384 268 1917
Fragments arranged by size:
1917 1664 1384 867 268
AflII C'TTAA_G
Cuts at: 820 1017 2707 820
Size: 197 1690 4213
Fragments arranged by size:
4213 1690 197
AflIII A'CryG_T
Cuts at: 1203 2955 4999 5976 1203
Size: 1752 2044 977 1327
Fragments arranged by size:
2044 1752 1327 977
AgeI A'CCGG_T
Cuts at: 3362 4931 3362
Size: 1569 4531
AhdI GACnn_n'nnGTC
Cuts at: 4829 4829
Size: 6100
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2691 1184
Size: 1507 4593
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2962 2994 2962
Size: 32 6068
AlwNI CAG_nnn'CTG
Cuts at: 2990 3843 5415 2990
Size: 853 1572 3675
Fragments arranged by size:
3675 1572 853
ApaI G_GGCC'C
Cuts at: 2802 2802
Size: 6100
ApaLI G'TGCA_C
Cuts at: 4157 5313 4157
Size: 1156 4944
ApoI r'AATT_y
Cuts at: 1493 3409 3635 3787 5896 1493
Size: 1916 226 152 2109 1697
Fragments arranged by size:
2109 1916 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2273 2333 2966 3165 3310 3383
Size: 73 123 207 60 633 199 145 73
Cuts at: 3383 3425 1870
Size: 42 4545
Fragments arranged by size:
4545 633 207 199 145 123 73 73
60 42
BamHI G'GATC_C
Cuts at: 2496 3430 3797 2496
Size: 934 367 4799
Fragments arranged by size:
4799 934 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2070 3346 3421 4876 618
Size: 325 217 627 283 1276 75 1455 1842
Fragments arranged by size:
1842 1455 1276 627 325 283 217 75
BanII G_rGCy'C
Cuts at: 1391 2802 2945 3164 3224 3419 1391
Size: 1411 143 219 60 195 4072
Fragments arranged by size:
4072 1411 219 195 143 60
BbeI G_GCGC'C
Cuts at: 2074 3350 2074
Size: 1276 4824
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3011 3045 4291 4325 1903
Size: 34 50 34 990 34 1246 34 3678
Fragments arranged by size:
3678 1246 990 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2701 4711
Size: 107 122 71 631 605 356 673 2010
Cuts at: 4711 136
Size: 1525
Fragments arranged by size:
2010 1525 673 631 605 356 122 107
71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 3517 3517
Size: 6100
Bme1580I G_kGCm'C
Cuts at: 1790 2706 2802 3082 4161 5317 1790
Size: 916 96 280 1079 1156 2573
Fragments arranged by size:
2573 1156 1079 916 280 96
BmgBI CAC'GTC
Cuts at: 1318 2146 2956 3201 1318
Size: 828 810 245 4217
Fragments arranged by size:
4217 828 810 245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3831 4789 442
Size: 416 53 83 399 24 2414 958 1753
Fragments arranged by size:
2414 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2843 3165 4760 1239
Size: 1604 322 1595 2579
Fragments arranged by size:
2579 1604 1595 322
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4225 5090 5388 4225
Size: 865 298 4937
Fragments arranged by size:
4937 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4763 882
Size: 617 3264 2219
Fragments arranged by size:
3264 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3891 493
Size: 711 2687 2702
Fragments arranged by size:
2702 2687 711
BsaBI GATnn'nnATC
Cuts at: 2610 6094 2610
Size: 3484 2616
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2071 3347 3907 4289
Size: 53 83 186 936 538 1276 560 382
Cuts at: 4289 4924 275
Size: 635 1451
Fragments arranged by size:
1451 1276 936 635 560 538 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2635 2642 3323 3362 4533 4931 5205 5352 5763
Size: 7 681 39 1171 398 274 147 411
Cuts at: 5763 2635
Size: 2972
Fragments arranged by size:
2972 1171 681 411 398 274 147 39
7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2869 2899 3179 3209 1178
Size: 30 165 30 1466 30 280 30 4069
Fragments arranged by size:
4069 1466 280 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2249 3076 3342 1186
Size: 1063 827 266 3944
Fragments arranged by size:
3944 1063 827 266
BseYI C'CCAG_C
Cuts at: 2074 2260 2831 2983 5303 5799 2074
Size: 186 571 152 2320 496 2375
Fragments arranged by size:
2375 2320 571 496 186 152
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3170 3308 1163
Size: 573 1434 138 3955
Fragments arranged by size:
3955 1434 573 138
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 2052 3550 4311 5339 1540
Size: 275 186 47 4 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186 47 4
BsiHKAI G_wGCw'C
Cuts at: 2394 2549 3302 4161 4246 5317 2394
Size: 155 753 859 85 1071 3177
Fragments arranged by size:
3177 1071 859 753 155 85
BsmI GAATG_Cn'
Cuts at: 3331 3611 3704 3331
Size: 280 93 5727
Fragments arranged by size:
5727 280 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2394 2549 2706 2802 2945 3082 3164
Size: 399 604 155 157 96 143 137 82
Cuts at: 3164 3224 3302 3419 4161 4246 5317 1391
Size: 60 78 117 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 604 399 157 155 143
137 117 96 85 82 78 60
BspEI T'CCGG_A
Cuts at: 2635 2642 5763 2635
Size: 7 3121 2972
Fragments arranged by size:
3121 2972 7
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 3455 844
Size: 1340 1271 3489
Fragments arranged by size:
3489 1340 1271
BsrBI CCG'CTC
Cuts at: 1394 1823 2239 2524 3987 1394
Size: 429 416 285 1463 3507
Fragments arranged by size:
3507 1463 429 416 285
BsrDI GCAATG_nn'
Cuts at: 65 1248 4595 4769 65
Size: 1183 3347 174 1396
Fragments arranged by size:
3347 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2047 3362 4744 4931 1700
Size: 110 237 1315 1382 187 2869
Fragments arranged by size:
2869 1382 1315 237 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6100
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 2854 1184
Size: 1670 4430
BstXI CCAn_nnnn'nTGG
Cuts at: 3025 3025
Size: 6100
BstYI r'GATC_y
Cuts at: 1972 2418 2496 2605 3430 3797 4182 4199 5651
Size: 446 78 109 825 367 385 17 1452
Cuts at: 5651 5737 5749 6095 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 825 446 385 367 346 109
86 78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2042 3259 513
Size: 552 977 1217 3354
Fragments arranged by size:
3354 1217 977 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 2132 2690 3082 3616 4410 4430 2132
Size: 558 392 534 794 20 3802
Fragments arranged by size:
3802 794 558 534 392 20
ClaI AT'CG_AT
Cuts at: 1554 2922 1554
Size: 1368 4732
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6065
DraI TTT'AAA
Cuts at: 3404 3404
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2045 2049 2307
Size: 54 1425 50 275 18 215 4 258
Cuts at: 2307 3547 4436 6065 8
Size: 1240 889 1629 43
Fragments arranged by size:
1629 1425 1240 889 275 258 215 54
50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2045 2049 3547 1537
Size: 275 233 4 1498 4090
Fragments arranged by size:
4090 1498 275 233 4
EarI CTCTTCn'nnn_
Cuts at: 1353 4030 1353
Size: 2677 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2440 2831 4687 5061 5207 387
Size: 1064 138 851 391 1856 374 146 1280
Fragments arranged by size:
1856 1280 1064 851 391 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2843 3165 3296 4163 4760 5547 5815
Size: 393 1211 322 131 867 597 787 268
Cuts at: 5815 1239
Size: 1524
Fragments arranged by size:
1524 1211 867 787 597 393 322 268
131
EcoNI CCTnn'n_nnAGG
Cuts at: 2102 2441 2102
Size: 339 5761
EcoO109I rG'GnC_Cy
Cuts at: 2798 2798
Size: 6100
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3085 3085
Size: 6100
FseI GG_CCGG'CC
Cuts at: 2051 2051
Size: 6100
FspI TGC'GCA
Cuts at: 2318 4606 2318
Size: 2288 3812
HaeII r_GCGC'y
Cuts at: 1678 2074 2911 3350 5247 1678
Size: 396 837 439 1897 2531
Fragments arranged by size:
2531 1897 837 439 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2577 2609 4741 4773 4815
Size: 32 135 32 1005 32 2132 32 42
Cuts at: 4815 4847 1373
Size: 32 2626
Fragments arranged by size:
2626 2132 1005 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3690 669
Size: 988 281 1506 246 3079
Fragments arranged by size:
3079 1506 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KasI G'GCGC_C
Cuts at: 2070 3346 2070
Size: 1276 4824
KpnI G_GTAC'C
Cuts at: 3425 3425
Size: 6100
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 5214 5398 1305
Size: 1116 2793 184 2007
Fragments arranged by size:
2793 2007 1116 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2309 10
Size: 54 1425 343 477 3801
Fragments arranged by size:
3801 1425 477 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4058 4417 4576 518
Size: 648 2892 359 159 2042
Fragments arranged by size:
2892 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2044 2990 3009 3130 3516 4193 5341 5586
Size: 438 946 19 121 386 677 1148 245
Cuts at: 5586 1606
Size: 2120
Fragments arranged by size:
2120 1148 946 677 438 386 245 121
19
NaeI GCC'GGC
Cuts at: 2049 2049
Size: 6100
NarI GG'CG_CC
Cuts at: 2071 3347 2071
Size: 1276 4824
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5548
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NgoMIV G'CCGG_C
Cuts at: 2047 2047
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 3458 5003 1132
Size: 97 2229 1545 2229
Fragments arranged by size:
2229 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2860 1348
Size: 1512 4588
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2990 3012 1108
Size: 1882 22 4196
Fragments arranged by size:
4196 1882 22
PfoI T'CCnGG_A
Cuts at: 2715 2715
Size: 6100
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4169 4201 5682 5714 4169
Size: 32 1481 32 4555
Fragments arranged by size:
4555 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspOMI G'GGCC_C
Cuts at: 2798 2798
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2181 3077 3452 830
Size: 1351 896 375 3478
Fragments arranged by size:
3478 1351 896 375
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2990 2990
Size: 6100
SacII CC_GC'GG
Cuts at: 2045 2045
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SbfI CC_TGCA'GG
Cuts at: 2181 3452 2181
Size: 1271 4829
ScaI AGT'ACT
Cuts at: 2198 2512 4348 6060 2198
Size: 314 1836 1712 2238
Fragments arranged by size:
2238 1836 1712 314
SexAI A'CCwGG_T
Cuts at: 2165 2469 2850 2165
Size: 304 381 5415
Fragments arranged by size:
5415 381 304
SfcI C'TryA_G
Cuts at: 826 1045 2177 3073 3448 4583 5264 5455 826
Size: 219 1132 896 375 1135 681 191 1471
Fragments arranged by size:
1471 1135 1132 896 681 375 219 191
SfoI GGC'GCC
Cuts at: 2072 3348 2072
Size: 1276 4824
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 2068 2968 3167 3385 3427 1872
Size: 196 900 199 218 42 4545
Fragments arranged by size:
4545 900 218 199 196 42
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2707 4204 5105 5367 820
Size: 197 926 764 1497 901 262 1553
Fragments arranged by size:
1553 1497 926 901 764 262 197
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6100
SphI G_CATG'C
Cuts at: 1132 3458 1132
Size: 2326 3774
SrfI GCCC'GGGC
Cuts at: 2068 2068
Size: 6100
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6100
StyI C'CwwG_G
Cuts at: 513 1065 3539 513
Size: 552 2474 3074
Fragments arranged by size:
3074 2474 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2117 3134 4127 4144 4297 4482 4927
Size: 1255 537 1017 993 17 153 185 445
Cuts at: 4927 325
Size: 1498
Fragments arranged by size:
1498 1255 1017 993 537 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2082 2196 2510
Size: 274 80 33 51 1443 105 114 314
Cuts at: 2510 4346 6058 96
Size: 1836 1712 138
Fragments arranged by size:
1836 1712 1443 314 274 138 114 105
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2869 2901 3179 3211 6060 6092 2869
Size: 32 278 32 2849 32 2877
Fragments arranged by size:
2877 2849 278 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2471 1316
Size: 1155 4945
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 2809
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6100
XmaI C'CCGG_G
Cuts at: 1870 2066 2966 3165 3383 3425 1870
Size: 196 900 199 218 42 4545
Fragments arranged by size:
4545 900 218 199 196 42
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3908 276
Size: 53 83 186 936 2374 2468
Fragments arranged by size:
2468 2374 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI
RsrII SacI SanDI SapI SfiI SwaI