Restriction map for
pFN21AB9448
(Circular) (Six-base) MAPSORT of: pFN21AB9448.seq Check: 9125 from: 1 to: 6166
pFN21AB9448 6166 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3976 278
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3509 1937
Size: 1572 4594
Acc65I G'GTAC_C
Cuts at: 3487 3487
Size: 6166
AclI AA'CG_TT
Cuts at: 1563 4293 4666 1563
Size: 2730 373 3063
Fragments arranged by size:
3063 2730 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3474 4229 5613 5881 1632
Size: 1842 755 1384 268 1917
Fragments arranged by size:
1917 1842 1384 755 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5969
AflIII A'CryG_T
Cuts at: 1203 5065 6042 1203
Size: 3862 977 1327
Fragments arranged by size:
3862 1327 977
AgeI A'CCGG_T
Cuts at: 4997 4997
Size: 6166
AhdI GACnn_n'nnGTC
Cuts at: 4895 4895
Size: 6166
AleI CACnn'nnGTG
Cuts at: 1166 2408 1166
Size: 1242 4924
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6166
AlwNI CAG_nnn'CTG
Cuts at: 2260 2484 3452 3909 5481 2260
Size: 224 968 457 1572 2945
Fragments arranged by size:
2945 1572 968 457 224
ApaLI G'TGCA_C
Cuts at: 4223 5379 4223
Size: 1156 5010
ApoI r'AATT_y
Cuts at: 1493 2748 3475 3701 3853 5962 1493
Size: 1255 727 226 152 2109 1697
Fragments arranged by size:
2109 1697 1255 727 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4720 160
Size: 872 3688 1606
Fragments arranged by size:
3688 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
AvaI C'yCGr_G
Cuts at: 1870 1943 2364 2919 3491 1870
Size: 73 421 555 572 4545
Fragments arranged by size:
4545 572 555 421 73
BamHI G'GATC_C
Cuts at: 3496 3863 3496
Size: 367 5799
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3487 4942 618
Size: 325 217 627 1700 1455 1842
Fragments arranged by size:
1842 1700 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2543 2826 3212 3485 1391
Size: 1152 283 386 273 4072
Fragments arranged by size:
4072 1152 386 283 273
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5195
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4357 4391 1903
Size: 34 2420 34 3678
Fragments arranged by size:
3678 2420 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4058 5268 4058
Size: 1210 4956
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6166
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4777 136
Size: 107 122 71 631 605 3105 1525
Fragments arranged by size:
3105 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6161 6161
Size: 6166
BlpI GC'TnA_GC
Cuts at: 3338 3583 3338
Size: 245 5921
Bme1580I G_kGCm'C
Cuts at: 1790 4227 5383 1790
Size: 2437 1156 2573
Fragments arranged by size:
2573 2437 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6166
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2236 3348 3897
Size: 416 53 83 399 24 819 1112 549
Cuts at: 3897 4855 442
Size: 958 1753
Fragments arranged by size:
1753 1112 958 819 549 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2184 1056
Size: 1128 5038
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4826 1239
Size: 3587 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4291 5156 5454 4291
Size: 865 298 5003
Fragments arranged by size:
5003 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4829 882
Size: 617 3330 2219
Fragments arranged by size:
3330 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3957 493
Size: 711 2753 2702
Fragments arranged by size:
2753 2702 711
BsaBI GATnn'nnATC
Cuts at: 2727 6160 2727
Size: 3433 2733
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3973 4355 4990 275
Size: 53 83 186 936 2440 382 635 1451
Fragments arranged by size:
2440 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4599 4997 5271 5418 5829 4599
Size: 398 274 147 411 4936
Fragments arranged by size:
4936 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2507 2525 2537 2555 2999
Size: 30 165 30 1104 18 12 18 444
Cuts at: 2999 3029 1178
Size: 30 4315
Fragments arranged by size:
4315 1104 444 165 30 30 30 18
18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2159 2756 3193 1186
Size: 973 597 437 4159
Fragments arranged by size:
4159 973 597 437
BseYI C'CCAG_C
Cuts at: 2323 2527 5369 5865 2323
Size: 204 2842 496 2624
Fragments arranged by size:
2842 2624 496 204
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 1163
Size: 573 696 4897
Fragments arranged by size:
4897 696 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3616 4377 5405 1540
Size: 275 186 1615 761 1028 2301
Fragments arranged by size:
2301 1615 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2517 2826 3212 3222 4227 4312 5383 2517
Size: 309 386 10 1005 85 1071 3300
Fragments arranged by size:
3300 1071 1005 386 309 85 10
BsmI GAATG_Cn'
Cuts at: 3291 3677 3770 3291
Size: 386 93 5687
Fragments arranged by size:
5687 386 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2517 2543 2826 3212 3222 3485 4227
Size: 399 727 26 283 386 10 263 742
Cuts at: 4227 4312 5383 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 727 399 386 283 263
85 26 10
BspEI T'CCGG_A
Cuts at: 5829 5829
Size: 6166
BspHI T'CATG_A
Cuts at: 2818 4055 5785 2818
Size: 1237 1730 3199
Fragments arranged by size:
3199 1730 1237
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3521 844
Size: 2677 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2617 4053 1394
Size: 429 794 1436 3507
Fragments arranged by size:
3507 1436 794 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2365 4661 4835 65
Size: 1183 1117 2296 174 1396
Fragments arranged by size:
2296 1396 1183 1117 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4810 4997 1700
Size: 110 3000 187 2869
Fragments arranged by size:
3000 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6166
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6166
BssSI C'ACGA_G
Cuts at: 2168 4226 5238 2168
Size: 2058 1012 3096
Fragments arranged by size:
3096 2058 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6166
BstBI TT'CG_AA
Cuts at: 4970 4970
Size: 6166
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6166
BstXI CCAn_nnnn'nTGG
Cuts at: 2029 2105 2029
Size: 76 6090
BstYI r'GATC_y
Cuts at: 1972 3496 3863 4248 4265 5717 5803 5815 6161
Size: 1524 367 385 17 1452 86 12 346
Cuts at: 6161 1972
Size: 1977
Fragments arranged by size:
1977 1524 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4675
Fragments arranged by size:
4675 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5427
Fragments arranged by size:
5427 683 56
BtsI GCAGTG_nn'
Cuts at: 2456 2572 2630 3682 4476 4496 2456
Size: 116 58 1052 794 20 4126
Fragments arranged by size:
4126 1052 794 116 58 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6166
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6131
DraI TTT'AAA
Cuts at: 2379 3396 3470 2379
Size: 1017 74 5075
Fragments arranged by size:
5075 1017 74
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5173 809
Size: 507 3857 1802
Fragments arranged by size:
3857 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2688 3613 4502
Size: 54 1425 50 275 18 858 925 889
Cuts at: 4502 6131 8
Size: 1629 43
Fragments arranged by size:
1629 1425 925 889 858 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3613 1537
Size: 275 1801 4090
Fragments arranged by size:
4090 1801 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2136 2878 3053 3210 4096 1353
Size: 783 742 175 157 886 3423
Fragments arranged by size:
3423 886 783 742 175 157
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4753 5127 5273 387
Size: 1064 138 3164 374 146 1280
Fragments arranged by size:
3164 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2824 3210 2824
Size: 386 5780
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3474 4229 4826 5613 5881 1239
Size: 393 1842 755 597 787 268 1524
Fragments arranged by size:
1842 1524 787 755 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2020 2020
Size: 6166
EcoO109I rG'GnC_Cy
Cuts at: 2025 2025
Size: 6166
EcoRI G'AATT_C
Cuts at: 3475 3853 3475
Size: 378 5788
FspI TGC'GCA
Cuts at: 4672 4672
Size: 6166
HaeII r_GCGC'y
Cuts at: 1678 2576 5313 1678
Size: 898 2737 2531
Fragments arranged by size:
2737 2531 898
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2214 2246 2525 2557 2969
Size: 32 135 32 642 32 279 32 412
Cuts at: 2969 3001 3350 3382 4807 4839 4881 4913 1373
Size: 32 349 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 642 412 349 279 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3510 3756 669
Size: 988 281 1572 246 3079
Fragments arranged by size:
3079 1572 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6166
HpaI GTT'AAC
Cuts at: 3756 3756
Size: 6166
KpnI G_GTAC'C
Cuts at: 3491 3491
Size: 6166
MfeI C'AATT_G
Cuts at: 3765 3765
Size: 6166
MluI A'CGCG_T
Cuts at: 6042 6042
Size: 6166
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2122 5280 5464 1305
Size: 817 3158 184 2007
Fragments arranged by size:
3158 2007 817 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2690 10
Size: 54 1425 343 858 3486
Fragments arranged by size:
3486 1425 858 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2317 2408 4124 4483 4642 518
Size: 648 1151 91 1716 359 159 2042
Fragments arranged by size:
2042 1716 1151 648 359 159 91
MspA1I CmG'CkG
Cuts at: 1606 2044 3582 4259 5407 5652 1606
Size: 438 1538 677 1148 245 2120
Fragments arranged by size:
2120 1538 1148 677 438 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4675
Fragments arranged by size:
4675 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6166
NheI G'CTAG_C
Cuts at: 1052 2180 1052
Size: 1128 5038
NotI GC'GGCC_GC
Cuts at: 3613 3613
Size: 6166
NspI r_CATG'y
Cuts at: 1132 1229 3173 3524 5069 1132
Size: 97 1944 351 1545 2229
Fragments arranged by size:
2229 1944 1545 351 97
PasI CC'CwG_GG
Cuts at: 1348 2029 2240 1348
Size: 681 211 5274
Fragments arranged by size:
5274 681 211
PciI A'CATG_T
Cuts at: 5065 5065
Size: 6166
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2484 1108
Size: 1376 4790
PfoI T'CCnGG_A
Cuts at: 3027 3027
Size: 6166
PmeI GTTT'AAAC
Cuts at: 3470 3470
Size: 6166
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4235 4267 5748 5780 4235
Size: 32 1481 32 4621
Fragments arranged by size:
4621 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2025 2025
Size: 6166
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6166
PsiI TTA'TAA
Cuts at: 3736 3736
Size: 6166
PstI C_TGCA'G
Cuts at: 830 2211 2339 2463 3168 3193 3518 830
Size: 1381 128 124 705 25 325 3478
Fragments arranged by size:
3478 1381 705 325 128 124 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6166
PvuII CAG'CTG
Cuts at: 2044 2044
Size: 6166
SacI G_AGCT'C
Cuts at: 2826 3212 2826
Size: 386 5780
SalI G'TCGA_C
Cuts at: 1936 3508 1936
Size: 1572 4594
SapI GCTCTTCn'nnn_
Cuts at: 3210 3210
Size: 6166
SbfI CC_TGCA'GG
Cuts at: 3518 3518
Size: 6166
ScaI AGT'ACT
Cuts at: 4414 6126 4414
Size: 1712 4454
SfcI C'TryA_G
Cuts at: 826 1045 2207 2335 2459 3164 3189 3514 4649
Size: 219 1162 128 124 705 25 325 1135
Cuts at: 4649 5330 5521 826
Size: 681 191 1471
Fragments arranged by size:
1471 1162 1135 705 681 325 219 191
128 124 25
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6166
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6166
SmaI CCC'GGG
Cuts at: 1872 2366 3493 1872
Size: 494 1127 4545
Fragments arranged by size:
4545 1127 494
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2919 4270 5171 5433 820
Size: 197 926 976 1351 901 262 1553
Fragments arranged by size:
1553 1351 976 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3957 493
Size: 3464 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6166
SphI G_CATG'C
Cuts at: 1132 3173 3524 1132
Size: 2041 351 3774
Fragments arranged by size:
3774 2041 351
SspI AAT'ATT
Cuts at: 5 52 2055 4090 5
Size: 47 2003 2035 2081
Fragments arranged by size:
2081 2035 2003 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6166
StyI C'CwwG_G
Cuts at: 513 1065 2004 2901 3385 3605 513
Size: 552 939 897 484 220 3074
Fragments arranged by size:
3074 939 897 552 484 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4193 4210 4363 4548 4993 325
Size: 1255 2613 17 153 185 445 1498
Fragments arranged by size:
2613 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4412 6124 96
Size: 274 80 33 51 1443 2435 1712 138
Fragments arranged by size:
2435 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2507 2539 2746 2778 6126 6158 2507
Size: 32 207 32 3348 32 2515
Fragments arranged by size:
3348 2515 207 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 1316
Size: 912 5254
XbaI T'CTAG_A
Cuts at: 3502 3502
Size: 6166
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2683 2683
Size: 6166
XhoI C'TCGA_G
Cuts at: 1943 2919 1943
Size: 976 5190
XmaI C'CCGG_G
Cuts at: 1870 2364 3491 1870
Size: 494 1127 4545
Fragments arranged by size:
4545 1127 494
XmnI GAAnn'nnTTC
Cuts at: 4295 4295
Size: 6166
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3974 276
Size: 53 83 186 936 2440 2468
Fragments arranged by size:
2468 2440 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI