Restriction map for
pFN21AB9446
(Circular) (Six-base) MAPSORT of: pFN21AB9446.seq Check: 5053 from: 1 to: 5674
pFN21AB9446 5674 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3484 278
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3017 1937
Size: 1080 4594
Acc65I G'GTAC_C
Cuts at: 2693 2995 2693
Size: 302 5372
AclI AA'CG_TT
Cuts at: 1563 3801 4174 1563
Size: 2238 373 3063
Fragments arranged by size:
3063 2238 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2508 3737 5121 5389 1632
Size: 876 1229 1384 268 1917
Fragments arranged by size:
1917 1384 1229 876 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5477
AflIII A'CryG_T
Cuts at: 1203 4573 5550 1203
Size: 3370 977 1327
Fragments arranged by size:
3370 1327 977
AgeI A'CCGG_T
Cuts at: 4505 4505
Size: 5674
AhdI GACnn_n'nnGTC
Cuts at: 2301 4403 2301
Size: 2102 3572
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5674
AlwNI CAG_nnn'CTG
Cuts at: 3417 4989 3417
Size: 1572 4102
ApaI G_GGCC'C
Cuts at: 2419 2419
Size: 5674
ApaLI G'TGCA_C
Cuts at: 3731 4887 3731
Size: 1156 4518
ApoI r'AATT_y
Cuts at: 1493 2033 2711 2736 2905 2983 3209 3361 5470
Size: 540 678 25 169 78 226 152 2109
Cuts at: 5470 1493
Size: 1697
Fragments arranged by size:
2109 1697 678 540 226 169 152 78
25
AseI AT'TA_AT
Cuts at: 160 1032 4228 160
Size: 872 3196 1606
Fragments arranged by size:
3196 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
AvaI C'yCGr_G
Cuts at: 1870 1943 2418 2999 1870
Size: 73 475 581 4545
Fragments arranged by size:
4545 581 475 73
BamHI G'GATC_C
Cuts at: 3004 3371 3004
Size: 367 5307
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2693 2995 4450 618
Size: 325 217 627 221 685 302 1455 1842
Fragments arranged by size:
1842 1455 685 627 325 302 221 217
BanII G_rGCy'C
Cuts at: 1391 2419 2993 1391
Size: 1028 574 4072
Fragments arranged by size:
4072 1028 574
BbeI G_GCGC'C
Cuts at: 2012 2012
Size: 5674
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2300 928
Size: 971 401 4302
Fragments arranged by size:
4302 971 401
BbvCI CC'TCA_GC
Cuts at: 2394 2394
Size: 5674
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2040 2074 3865 3899 1903
Size: 34 103 34 1791 34 3678
Fragments arranged by size:
3678 1791 103 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3566 4776 3566
Size: 1210 4464
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5674
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4285 136
Size: 107 122 71 631 605 2613 1525
Fragments arranged by size:
2613 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5669 5669
Size: 5674
BlpI GC'TnA_GC
Cuts at: 3091 3091
Size: 5674
Bme1580I G_kGCm'C
Cuts at: 1790 2419 3735 4891 1790
Size: 629 1316 1156 2573
Fragments arranged by size:
2573 1316 1156 629
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5674
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2527 3405 4363
Size: 416 53 83 399 24 1110 878 958
Cuts at: 4363 442
Size: 1753
Fragments arranged by size:
1753 1110 958 878 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5674
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4334 1239
Size: 3095 2579
Bpu10I CC'TnA_GC
Cuts at: 2394 2394
Size: 5674
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2606 3799 4664 4962 2606
Size: 1193 865 298 3318
Fragments arranged by size:
3318 1193 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2381 2713 4337 882
Size: 617 882 332 1624 2219
Fragments arranged by size:
2219 1624 882 617 332
BsaAI yAC'GTr
Cuts at: 493 1204 2200 3465 493
Size: 711 996 1265 2702
Fragments arranged by size:
2702 1265 996 711
BsaBI GATnn'nnATC
Cuts at: 5668 5668
Size: 5674
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 3481 3863 4498
Size: 53 83 186 936 476 1472 382 635
Cuts at: 4498 275
Size: 1451
Fragments arranged by size:
1472 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2176 4107 4505 4779 4926 5337 2176
Size: 1931 398 274 147 411 2513
Fragments arranged by size:
2513 1931 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5449
Fragments arranged by size:
5449 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2391 2937 1186
Size: 1205 546 3923
Fragments arranged by size:
3923 1205 546
BseYI C'CCAG_C
Cuts at: 2697 4877 5373 2697
Size: 2180 496 2998
Fragments arranged by size:
2998 2180 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3124 3885 4913 1540
Size: 275 186 1123 761 1028 2301
Fragments arranged by size:
2301 1123 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3735 3820 4891 3735
Size: 85 1071 4518
Fragments arranged by size:
4518 1071 85
BsmI GAATG_Cn'
Cuts at: 3185 3278 3185
Size: 93 5581
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2419 2993 3735 3820 4891 1391
Size: 399 629 574 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 629 574 399 85
BspEI T'CCGG_A
Cuts at: 2176 5337 2176
Size: 3161 2513
BspHI T'CATG_A
Cuts at: 2454 3563 5293 2454
Size: 1109 1730 2835
Fragments arranged by size:
2835 1730 1109
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3029 844
Size: 2185 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3561 1394
Size: 429 1738 3507
Fragments arranged by size:
3507 1738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2826 4169 4343 65
Size: 1183 1578 1343 174 1396
Fragments arranged by size:
1578 1396 1343 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4318 4505 1700
Size: 110 2508 187 2869
Fragments arranged by size:
2869 2508 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5674
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5674
BssSI C'ACGA_G
Cuts at: 2613 3734 4746 2613
Size: 1121 1012 3541
Fragments arranged by size:
3541 1121 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5674
BstBI TT'CG_AA
Cuts at: 4478 4478
Size: 5674
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5674
BstXI CCAn_nnnn'nTGG
Cuts at: 2777 2777
Size: 5674
BstYI r'GATC_y
Cuts at: 1972 2425 3004 3371 3756 3773 5225 5311 5323
Size: 453 579 367 385 17 1452 86 12
Cuts at: 5323 5669 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 579 453 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2501 513
Size: 552 939 497 3686
Fragments arranged by size:
3686 939 552 497
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4935
Fragments arranged by size:
4935 683 56
BtsI GCAGTG_nn'
Cuts at: 3190 3984 4004 3190
Size: 794 20 4860
Fragments arranged by size:
4860 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5674
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5639
DraI TTT'AAA
Cuts at: 2978 2978
Size: 5674
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4681 809
Size: 507 3365 1802
Fragments arranged by size:
3365 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3121 4010 5639
Size: 54 1425 50 275 18 1291 889 1629
Cuts at: 5639 8
Size: 43
Fragments arranged by size:
1629 1425 1291 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3121 1537
Size: 275 1309 4090
Fragments arranged by size:
4090 1309 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2079 2484 2843 3604 1353
Size: 726 405 359 761 3423
Fragments arranged by size:
3423 761 726 405 359
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4261 4635 4781 387
Size: 1064 138 2672 374 146 1280
Fragments arranged by size:
2672 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2508 3737 4334 5121 5389 1239
Size: 393 876 1229 597 787 268 1524
Fragments arranged by size:
1524 1229 876 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2020 2415 2020
Size: 395 5279
EcoRI G'AATT_C
Cuts at: 2905 2983 3361 2905
Size: 78 378 5218
Fragments arranged by size:
5218 378 78
FspI TGC'GCA
Cuts at: 4180 4180
Size: 5674
HaeII r_GCGC'y
Cuts at: 1678 2012 2786 4821 1678
Size: 334 774 2035 2531
Fragments arranged by size:
2531 2035 774 334
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2287 2319 4315 4347 4389
Size: 32 135 32 715 32 1996 32 42
Cuts at: 4389 4421 1373
Size: 32 2626
Fragments arranged by size:
2626 1996 715 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3018 3264 669
Size: 988 281 1080 246 3079
Fragments arranged by size:
3079 1080 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5674
HpaI GTT'AAC
Cuts at: 3264 3264
Size: 5674
KasI G'GCGC_C
Cuts at: 2008 2008
Size: 5674
KpnI G_GTAC'C
Cuts at: 2697 2999 2697
Size: 302 5372
MfeI C'AATT_G
Cuts at: 3273 3273
Size: 5674
MluI A'CGCG_T
Cuts at: 5550 5550
Size: 5674
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2129 4788 4972 1305
Size: 824 2659 184 2007
Fragments arranged by size:
2659 2007 824 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3852
Fragments arranged by size:
3852 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 2754 3632 3991 4150 518
Size: 648 976 612 878 359 159 2042
Fragments arranged by size:
2042 976 878 648 612 359 159
MspA1I CmG'CkG
Cuts at: 1606 3090 3767 4915 5160 1606
Size: 1484 677 1148 245 2120
Fragments arranged by size:
2120 1484 1148 677 245
NarI GG'CG_CC
Cuts at: 2009 2009
Size: 5674
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4183
Fragments arranged by size:
4183 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5674
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5674
NotI GC'GGCC_GC
Cuts at: 3121 3121
Size: 5674
NspI r_CATG'y
Cuts at: 1132 1229 3032 4577 1132
Size: 97 1803 1545 2229
Fragments arranged by size:
2229 1803 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5674
PciI A'CATG_T
Cuts at: 4573 4573
Size: 5674
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5674
PfoI T'CCnGG_A
Cuts at: 2037 2037
Size: 5674
PmeI GTTT'AAAC
Cuts at: 2978 2978
Size: 5674
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3743 3775 5256 5288 3743
Size: 32 1481 32 4129
Fragments arranged by size:
4129 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2020 2020
Size: 5674
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5674
PsiI TTA'TAA
Cuts at: 3244 3244
Size: 5674
PspOMI G'GGCC_C
Cuts at: 2415 2415
Size: 5674
PstI C_TGCA'G
Cuts at: 830 3026 830
Size: 2196 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5674
SalI G'TCGA_C
Cuts at: 1936 3016 1936
Size: 1080 4594
SapI GCTCTTCn'nnn_
Cuts at: 2484 2843 2484
Size: 359 5315
SbfI CC_TGCA'GG
Cuts at: 3026 3026
Size: 5674
ScaI AGT'ACT
Cuts at: 2190 3922 5634 2190
Size: 1732 1712 2230
Fragments arranged by size:
2230 1732 1712
SfcI C'TryA_G
Cuts at: 826 1045 3022 4157 4838 5029 826
Size: 219 1977 1135 681 191 1471
Fragments arranged by size:
1977 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2010 2010
Size: 5674
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5674
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5674
SmaI CCC'GGG
Cuts at: 1872 2420 3001 1872
Size: 548 581 4545
Fragments arranged by size:
4545 581 548
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2585 3778 4679 4941 820
Size: 197 926 642 1193 901 262 1553
Fragments arranged by size:
1553 1193 926 901 642 262 197
SnaBI TAC'GTA
Cuts at: 493 3465 493
Size: 2972 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5674
SphI G_CATG'C
Cuts at: 1132 3032 1132
Size: 1900 3774
SspI AAT'ATT
Cuts at: 5 52 3598 5
Size: 47 3546 2081
Fragments arranged by size:
3546 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5674
StyI C'CwwG_G
Cuts at: 513 1065 2004 3113 513
Size: 552 939 1109 3074
Fragments arranged by size:
3074 1109 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2776 3701 3718 3871 4056 4501 325
Size: 1255 1196 925 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1196 925 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2188 3920 5632
Size: 274 80 33 51 1443 211 1732 1712
Cuts at: 5632 96
Size: 138
Fragments arranged by size:
1732 1712 1443 274 211 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5634 5666 5634
Size: 32 5642
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5674
XbaI T'CTAG_A
Cuts at: 3010 3010
Size: 5674
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5674
XmaI C'CCGG_G
Cuts at: 1870 2418 2999 1870
Size: 548 581 4545
Fragments arranged by size:
4545 581 548
XmnI GAAnn'nnTTC
Cuts at: 2129 2172 3803 2129
Size: 43 1631 4000
Fragments arranged by size:
4000 1631 43
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3482 276
Size: 53 83 186 936 1948 2468
Fragments arranged by size:
2468 1948 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SrfI SwaI XcmI