Restriction map for
pFN21AB9443
(Circular) (Six-base) MAPSORT of: pFN21AB9443.seq Check: 2998 from: 1 to: 5626
pFN21AB9443 5626 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3436 278
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2969 1937
Size: 1032 4594
Acc65I G'GTAC_C
Cuts at: 2947 2947
Size: 5626
AclI AA'CG_TT
Cuts at: 1563 3753 4126 1563
Size: 2190 373 3063
Fragments arranged by size:
3063 2190 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2282 2522 2891 3689 5073 5341 1632
Size: 515 135 240 369 798 1384 268 1917
Fragments arranged by size:
1917 1384 798 515 369 268 240 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5429
AflIII A'CryG_T
Cuts at: 1203 4525 5502 1203
Size: 3322 977 1327
Fragments arranged by size:
3322 1327 977
AgeI A'CCGG_T
Cuts at: 4457 4457
Size: 5626
AhdI GACnn_n'nnGTC
Cuts at: 4355 4355
Size: 5626
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5626
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2847 1184
Size: 1663 3963
AlwNI CAG_nnn'CTG
Cuts at: 3369 4941 3369
Size: 1572 4054
ApaLI G'TGCA_C
Cuts at: 3683 4839 3683
Size: 1156 4470
ApoI r'AATT_y
Cuts at: 1493 2935 3161 3313 5422 1493
Size: 1442 226 152 2109 1697
Fragments arranged by size:
2109 1697 1442 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4180 160
Size: 872 3148 1606
Fragments arranged by size:
3148 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
AvaI C'yCGr_G
Cuts at: 1870 1943 2347 2951 1870
Size: 73 404 604 4545
Fragments arranged by size:
4545 604 404 73
AvrII C'CTAG_G
Cuts at: 2911 2911
Size: 5626
BamHI G'GATC_C
Cuts at: 2956 3323 2956
Size: 367 5259
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2947 4402 618
Size: 325 217 627 1160 1455 1842
Fragments arranged by size:
1842 1455 1160 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2369 2657 2670 2813 2945 1391
Size: 978 288 13 143 132 4072
Fragments arranged by size:
4072 978 288 143 132 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2602 2851 928
Size: 971 703 249 3703
Fragments arranged by size:
3703 971 703 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3817 3851 1903
Size: 34 1880 34 3678
Fragments arranged by size:
3678 1880 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3518 4728 3518
Size: 1210 4416
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5626
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2180 4237 136
Size: 107 122 71 631 605 508 2057 1525
Fragments arranged by size:
2057 1525 631 605 508 122 107 71
BglII A'GATC_T
Cuts at: 2724 5621 2724
Size: 2897 2729
BlpI GC'TnA_GC
Cuts at: 3043 3043
Size: 5626
Bme1580I G_kGCm'C
Cuts at: 1790 2012 3687 4843 1790
Size: 222 1675 1156 2573
Fragments arranged by size:
2573 1675 1156 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3357 4315 442
Size: 416 53 83 399 24 1940 958 1753
Fragments arranged by size:
1940 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5626
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 5626
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 4286 1239
Size: 1415 1632 2579
Fragments arranged by size:
2579 1632 1415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2723 3751 4616 4914 2723
Size: 1028 865 298 3435
Fragments arranged by size:
3435 1028 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4289 882
Size: 617 2790 2219
Fragments arranged by size:
2790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3417 493
Size: 711 2213 2702
Fragments arranged by size:
2702 2213 711
BsaBI GATnn'nnATC
Cuts at: 5620 5620
Size: 5626
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2417 2441 3433 3815
Size: 53 83 186 936 884 24 992 382
Cuts at: 3815 4450 275
Size: 635 1451
Fragments arranged by size:
1451 992 936 884 635 382 186 83
53 24
BsaWI w'CCGG_w
Cuts at: 2037 4059 4457 4731 4878 5289 2037
Size: 2022 398 274 147 411 2374
Fragments arranged by size:
2374 2022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2711 2741 1178
Size: 30 165 30 1308 30 4063
Fragments arranged by size:
4063 1308 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2359 2362 2632 2897 1186
Size: 1173 3 270 265 3915
Fragments arranged by size:
3915 1173 270 265 3
BseYI C'CCAG_C
Cuts at: 2030 2301 2316 4829 5325 2030
Size: 271 15 2513 496 2331
Fragments arranged by size:
2513 2331 496 271 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 2310 1163
Size: 573 492 82 4479
Fragments arranged by size:
4479 573 492 82
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2091 3076 3837 4865 1540
Size: 275 186 90 985 761 1028 2301
Fragments arranged by size:
2301 1028 985 761 275 186 90
BsiHKAI G_wGCw'C
Cuts at: 2532 2670 2813 3687 3772 4843 2532
Size: 138 143 874 85 1071 3315
Fragments arranged by size:
3315 1071 874 143 138 85
BsmI GAATG_Cn'
Cuts at: 3137 3230 3137
Size: 93 5533
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2369 2532 2657 2670 2813 2945
Size: 399 222 357 163 125 13 143 132
Cuts at: 2945 3687 3772 4843 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 357 222 163 143
132 125 85 13
BspEI T'CCGG_A
Cuts at: 5289 5289
Size: 5626
BspHI T'CATG_A
Cuts at: 2805 3515 5245 2805
Size: 710 1730 3186
Fragments arranged by size:
3186 1730 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2981 844
Size: 2137 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2553 2737 2883 3513 1394
Size: 429 730 184 146 630 3507
Fragments arranged by size:
3507 730 630 429 184 146
BsrDI GCAATG_nn'
Cuts at: 65 1248 4121 4295 65
Size: 1183 2873 174 1396
Fragments arranged by size:
2873 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2311 2487 2596 4270 4457 1700
Size: 110 501 176 109 1674 187 2869
Fragments arranged by size:
2869 1674 501 187 176 110 109
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5626
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5626
BssSI C'ACGA_G
Cuts at: 2663 3686 4698 2663
Size: 1023 1012 3591
Fragments arranged by size:
3591 1023 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5626
BstBI TT'CG_AA
Cuts at: 4430 4430
Size: 5626
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5626
BstYI r'GATC_y
Cuts at: 1972 2724 2956 3323 3708 3725 5177 5263 5275
Size: 752 232 367 385 17 1452 86 12
Cuts at: 5275 5621 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 752 385 367 346 232 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2073 2517 513
Size: 552 939 69 444 3622
Fragments arranged by size:
3622 939 552 444 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4887
Fragments arranged by size:
4887 683 56
BtsI GCAGTG_nn'
Cuts at: 3142 3936 3956 3142
Size: 794 20 4812
Fragments arranged by size:
4812 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5626
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2035 2070 529
Size: 35 1471 35 4085
Fragments arranged by size:
4085 1471 35 35
DraI TTT'AAA
Cuts at: 2930 2930
Size: 5626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4633 809
Size: 507 3317 1802
Fragments arranged by size:
3317 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2067 2088 2172
Size: 54 1425 50 275 18 237 21 84
Cuts at: 2172 2181 2334 2598 2884 3073 3962 5591 8
Size: 9 153 264 286 189 889 1629 43
Fragments arranged by size:
1629 1425 889 286 275 264 237 189
153 84 54 50 43 21 18 9
EagI C'GGCC_G
Cuts at: 1537 1812 2088 3073 1537
Size: 275 276 985 4090
Fragments arranged by size:
4090 985 276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2156 3556 1353
Size: 803 1400 3423
Fragments arranged by size:
3423 1400 803
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4213 4587 4733 387
Size: 1064 138 2624 374 146 1280
Fragments arranged by size:
2624 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2668 2811 2668
Size: 143 5483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2147 2282 2522 2654 2891 3689 4286
Size: 393 515 135 240 132 237 798 597
Cuts at: 4286 5073 5341 1239
Size: 787 268 1524
Fragments arranged by size:
1524 798 787 597 515 393 268 240
237 135 132
EcoRI G'AATT_C
Cuts at: 2935 3313 2935
Size: 378 5248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 5626
FspI TGC'GCA
Cuts at: 2540 4132 2540
Size: 1592 4034
HaeII r_GCGC'y
Cuts at: 1678 2134 4773 1678
Size: 456 2639 2531
Fragments arranged by size:
2639 2531 456
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 4267 4299 4341
Size: 32 135 32 1052 32 1611 32 42
Cuts at: 4341 4373 1373
Size: 32 2626
Fragments arranged by size:
2626 1611 1052 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2970 3216 669
Size: 988 281 1032 246 3079
Fragments arranged by size:
3079 1032 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5626
HpaI GTT'AAC
Cuts at: 3216 3216
Size: 5626
KpnI G_GTAC'C
Cuts at: 2951 2951
Size: 5626
MfeI C'AATT_G
Cuts at: 3225 3225
Size: 5626
MluI A'CGCG_T
Cuts at: 5502 5502
Size: 5626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2889 4740 4924 1305
Size: 1584 1851 184 2007
Fragments arranged by size:
2007 1851 1584 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3804
Fragments arranged by size:
3804 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2147 3584 3943 4102 518
Size: 648 981 1437 359 159 2042
Fragments arranged by size:
2042 1437 981 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3042 3719 4867 5112 1606
Size: 1436 677 1148 245 2120
Fragments arranged by size:
2120 1436 1148 677 245
NaeI GCC'GGC
Cuts at: 2598 2598
Size: 5626
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4135
Fragments arranged by size:
4135 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5626
NgoMIV G'CCGG_C
Cuts at: 2596 2596
Size: 5626
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5626
NotI GC'GGCC_GC
Cuts at: 3073 3073
Size: 5626
NspI r_CATG'y
Cuts at: 1132 1229 2623 2691 2984 4529 1132
Size: 97 1394 68 293 1545 2229
Fragments arranged by size:
2229 1545 1394 293 97 68
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5626
PciI A'CATG_T
Cuts at: 4525 4525
Size: 5626
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5626
PmeI GTTT'AAAC
Cuts at: 2930 2930
Size: 5626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3695 3727 5208 5240 3695
Size: 32 1481 32 4081
Fragments arranged by size:
4081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5626
PsiI TTA'TAA
Cuts at: 3196 3196
Size: 5626
PstI C_TGCA'G
Cuts at: 830 2568 2630 2826 2978 830
Size: 1738 62 196 152 3478
Fragments arranged by size:
3478 1738 196 152 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5626
SacI G_AGCT'C
Cuts at: 2670 2813 2670
Size: 143 5483
SalI G'TCGA_C
Cuts at: 1936 2968 1936
Size: 1032 4594
SbfI CC_TGCA'GG
Cuts at: 2978 2978
Size: 5626
ScaI AGT'ACT
Cuts at: 3874 5586 3874
Size: 1712 3914
SfcI C'TryA_G
Cuts at: 826 1045 2564 2626 2822 2974 4109 4790 4981
Size: 219 1519 62 196 152 1135 681 191
Cuts at: 4981 826
Size: 1471
Fragments arranged by size:
1519 1471 1135 681 219 196 191 152
62
SfiI GGCCn_nnn'nGGCC
Cuts at: 2180 2180
Size: 5626
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5626
SgrAI Cr'CCGG_yG
Cuts at: 1700 2487 1700
Size: 787 4839
SmaI CCC'GGG
Cuts at: 1872 2953 1872
Size: 1081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2738 3730 4631 4893 820
Size: 197 926 795 992 901 262 1553
Fragments arranged by size:
1553 992 926 901 795 262 197
SnaBI TAC'GTA
Cuts at: 493 3417 493
Size: 2924 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5626
SphI G_CATG'C
Cuts at: 1132 2984 1132
Size: 1852 3774
SspI AAT'ATT
Cuts at: 5 52 3550 5
Size: 47 3498 2081
Fragments arranged by size:
3498 2081 47
StuI AGG'CCT
Cuts at: 1517 2771 2916 1517
Size: 1254 145 4227
Fragments arranged by size:
4227 1254 145
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2156 2911 3065 513
Size: 552 939 108 44 755 154 3074
Fragments arranged by size:
3074 939 755 552 154 108 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2459 3653 3670 3823 4008 4453 325
Size: 1255 879 1194 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1194 879 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3872 5584 96
Size: 274 80 33 51 1443 1895 1712 138
Fragments arranged by size:
1895 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5586 5618 5586
Size: 32 5594
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5626
XbaI T'CTAG_A
Cuts at: 2962 2962
Size: 5626
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5626
XmaI C'CCGG_G
Cuts at: 1870 2951 1870
Size: 1081 4545
XmnI GAAnn'nnTTC
Cuts at: 3755 3755
Size: 5626
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3434 276
Size: 53 83 186 936 1900 2468
Fragments arranged by size:
2468 1900 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI
SwaI XcmI