Restriction map for
pFN21AB9440
(Circular) (Six-base) MAPSORT of: pFN21AB9440.seq Check: 9764 from: 1 to: 6685
pFN21AB9440 6685 bp
With 182 enzymes: SgfI *
May 17, 2016 16:10 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4495 278
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4028 1937
Size: 2091 4594
Acc65I G'GTAC_C
Cuts at: 4006 4006
Size: 6685
AclI AA'CG_TT
Cuts at: 1563 4812 5185 1563
Size: 3249 373 3063
Fragments arranged by size:
3249 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3155 3713 3983 4748 6132 6400 1632
Size: 1523 558 270 765 1384 268 1917
Fragments arranged by size:
1917 1523 1384 765 558 270 268
AfeI AGC'GCT
Cuts at: 2369 2917 2369
Size: 548 6137
AflII C'TTAA_G
Cuts at: 820 1017 3667 820
Size: 197 2650 3838
Fragments arranged by size:
3838 2650 197
AflIII A'CryG_T
Cuts at: 1203 5584 6561 1203
Size: 4381 977 1327
Fragments arranged by size:
4381 1327 977
AgeI A'CCGG_T
Cuts at: 5516 5516
Size: 6685
AhdI GACnn_n'nnGTC
Cuts at: 5414 5414
Size: 6685
AleI CACnn'nnGTG
Cuts at: 1166 2798 1166
Size: 1632 5053
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2342 1184
Size: 1158 5527
AlwNI CAG_nnn'CTG
Cuts at: 3812 4428 6000 3812
Size: 616 1572 4497
Fragments arranged by size:
4497 1572 616
ApaLI G'TGCA_C
Cuts at: 2279 2893 3329 4742 5898 2279
Size: 614 436 1413 1156 3066
Fragments arranged by size:
3066 1413 1156 614 436
ApoI r'AATT_y
Cuts at: 1493 2272 2690 3639 3994 4220 4372 6481 1493
Size: 779 418 949 355 226 152 2109 1697
Fragments arranged by size:
2109 1697 949 779 418 355 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5239 160
Size: 872 4207 1606
Fragments arranged by size:
4207 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
AvaI C'yCGr_G
Cuts at: 1870 1943 2084 2874 4010 1870
Size: 73 141 790 1136 4545
Fragments arranged by size:
4545 1136 790 141 73
BamHI G'GATC_C
Cuts at: 4015 4382 4015
Size: 367 6318
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2097 2925 4006 5461 618
Size: 325 217 627 310 828 1081 1455 1842
Fragments arranged by size:
1842 1455 1081 828 627 325 310 217
BanII G_rGCy'C
Cuts at: 1391 2881 4004 1391
Size: 1490 1123 4072
Fragments arranged by size:
4072 1490 1123
BbeI G_GCGC'C
Cuts at: 2101 2101
Size: 6685
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3215 928
Size: 971 1316 4398
Fragments arranged by size:
4398 1316 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4876 4910 1903
Size: 34 2939 34 3678
Fragments arranged by size:
3678 2939 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4577 5787 4577
Size: 1210 5475
BclI T'GATC_A
Cuts at: 1548 3322 1548
Size: 1774 4911
BfrBI ATG'CAT
Cuts at: 3830 3830
Size: 6685
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2539 5296 136
Size: 107 122 71 631 605 867 2757 1525
Fragments arranged by size:
2757 1525 867 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6680 6680
Size: 6685
BlpI GC'TnA_GC
Cuts at: 4102 4102
Size: 6685
Bme1580I G_kGCm'C
Cuts at: 1790 2283 2897 3333 4746 5902 1790
Size: 493 614 436 1413 1156 2573
Fragments arranged by size:
2573 1413 1156 614 493 436
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6685
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3375 4416 5374
Size: 416 53 83 399 24 1958 1041 958
Cuts at: 5374 442
Size: 1753
Fragments arranged by size:
1958 1753 1041 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6685
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3275 5345 1239
Size: 2036 2070 2579
Fragments arranged by size:
2579 2070 2036
Bpu10I CC'TnA_GC
Cuts at: 2295 3108 2295
Size: 813 5872
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4810 5675 5973 4810
Size: 865 298 5522
Fragments arranged by size:
5522 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3047 5348 882
Size: 617 1548 2301 2219
Fragments arranged by size:
2301 2219 1548 617
BsaAI yAC'GTr
Cuts at: 493 1204 2469 2800 4476 493
Size: 711 1265 331 1676 2702
Fragments arranged by size:
2702 1676 1265 711 331
BsaBI GATnn'nnATC
Cuts at: 6679 6679
Size: 6685
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2098 4492 4874 5509
Size: 53 83 186 936 565 2394 382 635
Cuts at: 5509 275
Size: 1451
Fragments arranged by size:
2394 1451 936 635 565 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3063 5118 5516 5790 5937 6348 3063
Size: 2055 398 274 147 411 3400
Fragments arranged by size:
3400 2055 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3664 3694 1178
Size: 30 165 30 2261 30 4169
Fragments arranged by size:
4169 2261 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3623 1186
Size: 2437 4248
BseYI C'CCAG_C
Cuts at: 2577 2982 5888 6384 2577
Size: 405 2906 496 2878
Fragments arranged by size:
2906 2878 496 405
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3495 1163
Size: 573 1759 4353
Fragments arranged by size:
4353 1759 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4135 4896 5924 1540
Size: 275 186 2134 761 1028 2301
Fragments arranged by size:
2301 2134 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2283 2897 3333 4746 4831 5902 2283
Size: 614 436 1413 85 1071 3066
Fragments arranged by size:
3066 1413 1071 614 436 85
BsmI GAATG_Cn'
Cuts at: 4196 4289 4196
Size: 93 6592
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 2881 2897 3333 4004 4746 4831
Size: 399 493 598 16 436 671 742 85
Cuts at: 4831 5902 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 671 598 493 436 399
85 16
BspEI T'CCGG_A
Cuts at: 6348 6348
Size: 6685
BspHI T'CATG_A
Cuts at: 3282 3741 4574 6304 3282
Size: 459 833 1730 3663
Fragments arranged by size:
3663 1730 833 459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 3384 3394 4040 844
Size: 1181 1359 10 646 3489
Fragments arranged by size:
3489 1359 1181 646 10
BsrBI CCG'CTC
Cuts at: 1394 1823 2029 4572 1394
Size: 429 206 2543 3507
Fragments arranged by size:
3507 2543 429 206
BsrDI GCAATG_nn'
Cuts at: 65 1248 2898 3211 3592 5180 5354 65
Size: 1183 1650 313 381 1588 174 1396
Fragments arranged by size:
1650 1588 1396 1183 381 313 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5329 5516 1700
Size: 110 3519 187 2869
Fragments arranged by size:
3519 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6685
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6685
BssSI C'ACGA_G
Cuts at: 4745 5757 4745
Size: 1012 5673
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6685
BstBI TT'CG_AA
Cuts at: 5489 5489
Size: 6685
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6685
BstYI r'GATC_y
Cuts at: 1972 2305 4015 4382 4767 4784 6236 6322 6334
Size: 333 1710 367 385 17 1452 86 12
Cuts at: 6334 6680 1972
Size: 346 1977
Fragments arranged by size:
1977 1710 1452 385 367 346 333 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2835 3423 513
Size: 552 1770 588 3775
Fragments arranged by size:
3775 1770 588 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2224 487
Size: 683 56 998 4948
Fragments arranged by size:
4948 998 683 56
BtsI GCAGTG_nn'
Cuts at: 3012 3223 3968 4201 4995 5015 3012
Size: 211 745 233 794 20 4682
Fragments arranged by size:
4682 794 745 233 211 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6685
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2949 2984 3408 3443 529
Size: 35 2385 35 424 35 3771
Fragments arranged by size:
3771 2385 424 35 35 35
DraI TTT'AAA
Cuts at: 2269 3989 2269
Size: 1720 4965
DraIII CAC_nnn'GTG
Cuts at: 3604 3604
Size: 6685
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5692 809
Size: 507 4376 1802
Fragments arranged by size:
4376 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3009 3416 3868
Size: 54 1425 50 275 18 1179 407 452
Cuts at: 3868 4132 5021 6650 8
Size: 264 889 1629 43
Fragments arranged by size:
1629 1425 1179 889 452 407 275 264
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4132 1537
Size: 275 2320 4090
Fragments arranged by size:
4090 2320 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4615 1353
Size: 3262 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2026 5272 5646 5792 387
Size: 1064 138 437 3246 374 146 1280
Fragments arranged by size:
3246 1280 1064 437 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3155 3275 3713 3983 4748 5345 6132
Size: 393 1523 120 438 270 765 597 787
Cuts at: 6132 6400 1239
Size: 268 1524
Fragments arranged by size:
1524 1523 787 765 597 438 393 270
268 120
EcoNI CCTnn'n_nnAGG
Cuts at: 2658 2658
Size: 6685
EcoO109I rG'GnC_Cy
Cuts at: 2643 2850 3091 2643
Size: 207 241 6237
Fragments arranged by size:
6237 241 207
EcoRI G'AATT_C
Cuts at: 3994 4372 3994
Size: 378 6307
EcoRV GAT'ATC
Cuts at: 3433 3433
Size: 6685
FspI TGC'GCA
Cuts at: 2021 5191 2021
Size: 3170 3515
HaeII r_GCGC'y
Cuts at: 1678 2101 2371 2919 5832 1678
Size: 423 270 548 2913 2531
Fragments arranged by size:
2913 2531 548 423 270
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2542 2574 3664 3696 3736
Size: 32 135 32 970 32 1090 32 40
Cuts at: 3736 3768 5326 5358 5400 5432 1373
Size: 32 1558 32 42 32 2626
Fragments arranged by size:
2626 1558 1090 970 135 42 40 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3568 4029 4275 669
Size: 988 281 1630 461 246 3079
Fragments arranged by size:
3079 1630 988 461 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6685
HpaI GTT'AAC
Cuts at: 4275 4275
Size: 6685
KasI G'GCGC_C
Cuts at: 2097 2097
Size: 6685
KpnI G_GTAC'C
Cuts at: 4010 4010
Size: 6685
MfeI C'AATT_G
Cuts at: 4284 4284
Size: 6685
MluI A'CGCG_T
Cuts at: 6561 6561
Size: 6685
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3161 5799 5983 1305
Size: 1856 2638 184 2007
Fragments arranged by size:
2638 2007 1856 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3011 3418 10
Size: 54 1425 343 1179 407 3277
Fragments arranged by size:
3277 1425 1179 407 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2213 2762 2798 3216 3281 4643 5002
Size: 648 1047 549 36 418 65 1362 359
Cuts at: 5002 5161 518
Size: 159 2042
Fragments arranged by size:
2042 1362 1047 648 549 418 359 159
65 36
MspA1I CmG'CkG
Cuts at: 1606 2809 3128 4101 4778 5926 6171 1606
Size: 1203 319 973 677 1148 245 2120
Fragments arranged by size:
2120 1203 1148 973 677 319 245
NarI GG'CG_CC
Cuts at: 2098 2098
Size: 6685
NcoI C'CATG_G
Cuts at: 513 1065 2835 3423 513
Size: 552 1770 588 3775
Fragments arranged by size:
3775 1770 588 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6685
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6685
NotI GC'GGCC_GC
Cuts at: 4132 4132
Size: 6685
NsiI A_TGCA'T
Cuts at: 3832 3832
Size: 6685
NspI r_CATG'y
Cuts at: 1132 1229 2323 2615 2761 3180 4043 5588 1132
Size: 97 1094 292 146 419 863 1545 2229
Fragments arranged by size:
2229 1545 1094 863 419 292 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6685
PciI A'CATG_T
Cuts at: 5584 5584
Size: 6685
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2526 3034 3604 1108
Size: 1418 508 570 4189
Fragments arranged by size:
4189 1418 570 508
PfoI T'CCnGG_A
Cuts at: 2070 3149 2070
Size: 1079 5606
PmeI GTTT'AAAC
Cuts at: 3989 3989
Size: 6685
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4754 4786 6267 6299 4754
Size: 32 1481 32 5140
Fragments arranged by size:
5140 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2643 2850 3091 2643
Size: 207 241 6237
Fragments arranged by size:
6237 241 207
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6685
PsiI TTA'TAA
Cuts at: 4255 4255
Size: 6685
PstI C_TGCA'G
Cuts at: 830 3975 4037 830
Size: 3145 62 3478
Fragments arranged by size:
3478 3145 62
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6685
SalI G'TCGA_C
Cuts at: 1936 4027 1936
Size: 2091 4594
SanDI GG'GwC_CC
Cuts at: 2643 2643
Size: 6685
SbfI CC_TGCA'GG
Cuts at: 4037 4037
Size: 6685
ScaI AGT'ACT
Cuts at: 4933 6645 4933
Size: 1712 4973
SfcI C'TryA_G
Cuts at: 826 1045 3971 4033 5168 5849 6040 826
Size: 219 2926 62 1135 681 191 1471
Fragments arranged by size:
2926 1471 1135 681 219 191 62
SfoI GGC'GCC
Cuts at: 2099 2099
Size: 6685
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6685
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6685
SmaI CCC'GGG
Cuts at: 1872 4012 1872
Size: 2140 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3667 4789 5690 5952 820
Size: 197 926 1724 1122 901 262 1553
Fragments arranged by size:
1724 1553 1122 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4476 493
Size: 3983 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6685
SphI G_CATG'C
Cuts at: 1132 4043 1132
Size: 2911 3774
SspI AAT'ATT
Cuts at: 5 52 3232 4609 5
Size: 47 3180 1377 2081
Fragments arranged by size:
3180 2081 1377 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6685
StyI C'CwwG_G
Cuts at: 513 1065 2835 3423 4124 513
Size: 552 1770 588 701 3074
Fragments arranged by size:
3074 1770 701 588 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4712 4729 4882 5067 5512 325
Size: 1255 3132 17 153 185 445 1498
Fragments arranged by size:
3132 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4931 6643 96
Size: 274 80 33 51 1443 2954 1712 138
Fragments arranged by size:
2954 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3175 3207 3330 3362 6645 6677 3175
Size: 32 123 32 3283 32 3183
Fragments arranged by size:
3283 3183 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6685
XbaI T'CTAG_A
Cuts at: 4021 4021
Size: 6685
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6685
XmaI C'CCGG_G
Cuts at: 1870 4010 1870
Size: 2140 4545
XmnI GAAnn'nnTTC
Cuts at: 2551 3140 3522 4814 2551
Size: 589 382 1292 4422
Fragments arranged by size:
4422 1292 589 382
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4493 276
Size: 53 83 186 936 2959 2468
Fragments arranged by size:
2959 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI
FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SapI SexAI SfiI
SrfI SwaI XcmI