Restriction map for
pFN21AB9439
(Circular) (Six-base) MAPSORT of: pFN21AB9439.seq Check: 2433 from: 1 to: 6094
pFN21AB9439 6094 bp
With 182 enzymes: SgfI *
August 16, 2010 14:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2380 2380
Size: 6094
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3904 278
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3437 1937
Size: 1500 4594
Acc65I G'GTAC_C
Cuts at: 3415 3415
Size: 6094
AclI AA'CG_TT
Cuts at: 1563 4221 4594 1563
Size: 2658 373 3063
Fragments arranged by size:
3063 2658 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2220 2647 4157 5541 5809 1632
Size: 588 427 1510 1384 268 1917
Fragments arranged by size:
1917 1510 1384 588 427 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5897
AflIII A'CryG_T
Cuts at: 1203 2715 4993 5970 1203
Size: 1512 2278 977 1327
Fragments arranged by size:
2278 1512 1327 977
AgeI A'CCGG_T
Cuts at: 4925 4925
Size: 6094
AhdI GACnn_n'nnGTC
Cuts at: 4823 4823
Size: 6094
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6094
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6094
AlwNI CAG_nnn'CTG
Cuts at: 2338 2383 3837 5409 2338
Size: 45 1454 1572 3023
Fragments arranged by size:
3023 1572 1454 45
ApaI G_GGCC'C
Cuts at: 3359 3359
Size: 6094
ApaLI G'TGCA_C
Cuts at: 4151 5307 4151
Size: 1156 4938
ApoI r'AATT_y
Cuts at: 1493 3403 3629 3781 5890 1493
Size: 1910 226 152 2109 1697
Fragments arranged by size:
2109 1910 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4648 160
Size: 872 3616 1606
Fragments arranged by size:
3616 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
AvaI C'yCGr_G
Cuts at: 1870 1943 2484 2537 3419 1870
Size: 73 541 53 882 4545
Fragments arranged by size:
4545 882 541 73 53
BamHI G'GATC_C
Cuts at: 3424 3791 3424
Size: 367 5727
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3000 3415 4870 618
Size: 325 217 627 1213 415 1455 1842
Fragments arranged by size:
1842 1455 1213 627 415 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 2467 2547 3359 3413 1391
Size: 638 438 80 812 54 4072
Fragments arranged by size:
4072 812 638 438 80 54
BbeI G_GCGC'C
Cuts at: 3004 3004
Size: 6094
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2208 928
Size: 971 309 4814
Fragments arranged by size:
4814 971 309
BbvCI CC'TCA_GC
Cuts at: 2244 2448 3169 2244
Size: 204 721 5169
Fragments arranged by size:
5169 721 204
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4285 4319 1903
Size: 34 2348 34 3678
Fragments arranged by size:
3678 2348 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3986 5196 3986
Size: 1210 4884
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6094
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2412 2838 3309
Size: 107 122 71 631 605 740 426 471
Cuts at: 3309 4705 136
Size: 1396 1525
Fragments arranged by size:
1525 1396 740 631 605 471 426 122
107 71
BglII A'GATC_T
Cuts at: 6089 6089
Size: 6094
BlpI GC'TnA_GC
Cuts at: 2804 3511 2804
Size: 707 5387
Bme1580I G_kGCm'C
Cuts at: 1790 3359 4155 5311 1790
Size: 1569 796 1156 2573
Fragments arranged by size:
2573 1569 1156 796
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2175 2202 2541
Size: 416 53 83 399 24 758 27 339
Cuts at: 2541 3825 4783 442
Size: 1284 958 1753
Fragments arranged by size:
1753 1284 958 758 416 399 339 83
53 27 24
BmtI G_CTAG'C
Cuts at: 1056 2303 1056
Size: 1247 4847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1999 2013 2075 2309 2315 2348 2576 2585
Size: 760 14 62 234 6 33 228 9
Cuts at: 2585 2759 3225 4754 1239
Size: 174 466 1529 2579
Fragments arranged by size:
2579 1529 760 466 234 228 174 62
33 14 9 6
Bpu10I CC'TnA_GC
Cuts at: 2244 2448 2702 3169 2244
Size: 204 254 467 5169
Fragments arranged by size:
5169 467 254 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2711 2764 3291 4219 5084 5382 2711
Size: 53 527 928 865 298 3423
Fragments arranged by size:
3423 928 865 527 298 53
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4757 882
Size: 617 3258 2219
Fragments arranged by size:
3258 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3885 493
Size: 711 2681 2702
Fragments arranged by size:
2702 2681 711
BsaBI GATnn'nnATC
Cuts at: 6088 6088
Size: 6094
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3001 3901 4283 4918
Size: 53 83 186 936 1468 900 382 635
Cuts at: 4918 275
Size: 1451
Fragments arranged by size:
1468 1451 936 900 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4527 4925 5199 5346 5757 4527
Size: 398 274 147 411 4864
Fragments arranged by size:
4864 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2304 2334 1178
Size: 30 165 30 901 30 4938
Fragments arranged by size:
4938 901 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2312 2555 2573 2945 1186
Size: 1126 243 18 372 4335
Fragments arranged by size:
4335 1126 372 243 18
BseYI C'CCAG_C
Cuts at: 2224 2253 2303 2397 2478 2605 2789 2813 3050
Size: 29 50 94 81 127 184 24 237
Cuts at: 3050 3191 3299 3347 5297 5793 2224
Size: 141 108 48 1950 496 2525
Fragments arranged by size:
2525 1950 496 237 184 141 127 108
94 81 50 48 29 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2942 1163
Size: 573 1206 4315
Fragments arranged by size:
4315 1206 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3544 4305 5333 1540
Size: 275 186 841 702 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 702 275 186
BsiHKAI G_wGCw'C
Cuts at: 2094 2467 3149 4155 4240 5311 2094
Size: 373 682 1006 85 1071 2877
Fragments arranged by size:
2877 1071 1006 682 373 85
BsmI GAATG_Cn'
Cuts at: 3605 3698 3605
Size: 93 6001
BsmBI CGTCTCn'nnnn_
Cuts at: 2839 2839
Size: 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2094 2467 2547 3149 3359 3413
Size: 399 239 65 373 80 602 210 54
Cuts at: 3413 4155 4240 5311 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 602 399 373 239 210
85 80 65 54
BspEI T'CCGG_A
Cuts at: 5757 5757
Size: 6094
BspHI T'CATG_A
Cuts at: 3983 5713 3983
Size: 1730 4364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2380 3449 844
Size: 1536 1069 3489
Fragments arranged by size:
3489 1536 1069
BsrBI CCG'CTC
Cuts at: 1394 1823 3981 1394
Size: 429 2158 3507
Fragments arranged by size:
3507 2158 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4589 4763 65
Size: 1183 3341 174 1396
Fragments arranged by size:
3341 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2248 2997 3064 4738 4925 1700
Size: 110 438 749 67 1674 187 2869
Fragments arranged by size:
2869 1674 749 438 187 110 67
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6094
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6094
BssSI C'ACGA_G
Cuts at: 4154 5166 4154
Size: 1012 5082
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2341 794
Size: 1547 4547
BstBI TT'CG_AA
Cuts at: 4898 4898
Size: 6094
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6094
BstXI CCAn_nnnn'nTGG
Cuts at: 2303 2518 2554 2303
Size: 215 36 5843
Fragments arranged by size:
5843 215 36
BstYI r'GATC_y
Cuts at: 1972 2934 3424 3791 4176 4193 5645 5731 5743
Size: 962 490 367 385 17 1452 86 12
Cuts at: 5743 6089 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 962 490 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2637 513
Size: 552 939 633 3970
Fragments arranged by size:
3970 939 633 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5355
Fragments arranged by size:
5355 683 56
BtsI GCAGTG_nn'
Cuts at: 3037 3610 4404 4424 3037
Size: 573 794 20 4707
Fragments arranged by size:
4707 794 573 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6094
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6059
DraI TTT'AAA
Cuts at: 3398 3398
Size: 6094
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5101 809
Size: 507 3785 1802
Fragments arranged by size:
3785 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2634 2839 3066
Size: 54 1425 50 275 18 804 205 227
Cuts at: 3066 3276 3541 4430 6059 8
Size: 210 265 889 1629 43
Fragments arranged by size:
1629 1425 889 804 275 265 227 210
205 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 3541 1537
Size: 275 1027 702 4090
Fragments arranged by size:
4090 1027 702 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2472 2849 2891 4024 1353
Size: 1119 377 42 1133 3423
Fragments arranged by size:
3423 1133 1119 377 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4681 5055 5201 387
Size: 1064 138 3092 374 146 1280
Fragments arranged by size:
3092 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2465 2465
Size: 6094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1999 2013 2075 2220 2309 2315 2348
Size: 393 367 14 62 145 89 6 33
Cuts at: 2348 2576 2585 2647 2759 3225 4157 4754 5541
Size: 228 9 62 112 466 932 597 787
Cuts at: 5541 5809 1239
Size: 268 1524
Fragments arranged by size:
1524 932 787 597 466 393 367 268
228 145 112 89 62 62 33 14
9 6
EcoO109I rG'GnC_Cy
Cuts at: 2692 3111 3356 2692
Size: 419 245 5430
Fragments arranged by size:
5430 419 245
EcoRI G'AATT_C
Cuts at: 3403 3781 3403
Size: 378 5716
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2626 2626
Size: 6094
FspI TGC'GCA
Cuts at: 4600 4600
Size: 6094
HaeII r_GCGC'y
Cuts at: 1678 3004 5241 1678
Size: 1326 2237 2531
Fragments arranged by size:
2531 2237 1326
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2416 2448 2513
Size: 32 135 32 497 32 315 32 65
Cuts at: 2513 2545 2753 2785 4735 4767 4809 4841 1373
Size: 32 208 32 1950 32 42 32 2626
Fragments arranged by size:
2626 1950 497 315 208 135 65 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2286 3438 3684 669
Size: 988 281 348 1152 246 3079
Fragments arranged by size:
3079 1152 988 348 281 246
HindIII A'AGCT_T
Cuts at: 748 3129 748
Size: 2381 3713
HpaI GTT'AAC
Cuts at: 3684 3684
Size: 6094
KasI G'GCGC_C
Cuts at: 3000 3000
Size: 6094
KpnI G_GTAC'C
Cuts at: 3419 3419
Size: 6094
MfeI C'AATT_G
Cuts at: 3693 3693
Size: 6094
MluI A'CGCG_T
Cuts at: 5970 5970
Size: 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5208 5392 1305
Size: 3903 184 2007
Fragments arranged by size:
3903 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 10
Size: 54 1425 343 804 3468
Fragments arranged by size:
3468 1425 804 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4052 4411 4570 518
Size: 648 2886 359 159 2042
Fragments arranged by size:
2886 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2338 2803 2956 3173 3203 3510 4187 5335
Size: 732 465 153 217 30 307 677 1148
Cuts at: 5335 5580 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 732 677 465 307 245 217
153 30
NaeI GCC'GGC
Cuts at: 2250 3066 2250
Size: 816 5278
NarI GG'CG_CC
Cuts at: 3001 3001
Size: 6094
NcoI C'CATG_G
Cuts at: 513 1065 2004 2637 513
Size: 552 939 633 3970
Fragments arranged by size:
3970 939 633 552
NdeI CA'TA_TG
Cuts at: 387 3084 387
Size: 2697 3397
NgoMIV G'CCGG_C
Cuts at: 2248 3064 2248
Size: 816 5278
NheI G'CTAG_C
Cuts at: 1052 2299 1052
Size: 1247 4847
NotI GC'GGCC_GC
Cuts at: 3541 3541
Size: 6094
NspI r_CATG'y
Cuts at: 1132 1229 2719 3452 4997 1132
Size: 97 1490 733 1545 2229
Fragments arranged by size:
2229 1545 1490 733 97
PasI CC'CwG_GG
Cuts at: 1348 2458 3056 1348
Size: 1110 598 4386
Fragments arranged by size:
4386 1110 598
PciI A'CATG_T
Cuts at: 2715 4993 2715
Size: 2278 3816
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 1108
Size: 1230 4864
PfoI T'CCnGG_A
Cuts at: 2736 2736
Size: 6094
PmeI GTTT'AAAC
Cuts at: 3398 3398
Size: 6094
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4163 4195 5676 5708 4163
Size: 32 1481 32 4549
Fragments arranged by size:
4549 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3111 3111
Size: 6094
PshAI GACnn'nnGTC
Cuts at: 1613 2876 1613
Size: 1263 4831
PsiI TTA'TAA
Cuts at: 3664 3664
Size: 6094
PspOMI G'GGCC_C
Cuts at: 3355 3355
Size: 6094
PstI C_TGCA'G
Cuts at: 830 3032 3446 830
Size: 2202 414 3478
Fragments arranged by size:
3478 2202 414
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6094
PvuII CAG'CTG
Cuts at: 2338 2803 2956 3173 3203 2338
Size: 465 153 217 30 5229
Fragments arranged by size:
5229 465 217 153 30
SacI G_AGCT'C
Cuts at: 2467 2467
Size: 6094
SalI G'TCGA_C
Cuts at: 1936 3436 1936
Size: 1500 4594
SapI GCTCTTCn'nnn_
Cuts at: 2472 2472
Size: 6094
SbfI CC_TGCA'GG
Cuts at: 3446 3446
Size: 6094
ScaI AGT'ACT
Cuts at: 4342 6054 4342
Size: 1712 4382
SfcI C'TryA_G
Cuts at: 826 1045 3028 3073 3442 4577 5258 5449 826
Size: 219 1983 45 369 1135 681 191 1471
Fragments arranged by size:
1983 1471 1135 681 369 219 191 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 2838 2838
Size: 6094
SfoI GGC'GCC
Cuts at: 3002 3002
Size: 6094
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6094
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6094
SmaI CCC'GGG
Cuts at: 1872 2486 3421 1872
Size: 614 935 4545
Fragments arranged by size:
4545 935 614
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 2779 3306 4198 5099 5361
Size: 197 926 783 53 527 892 901 262
Cuts at: 5361 820
Size: 1553
Fragments arranged by size:
1553 926 901 892 783 527 262 197
53
SnaBI TAC'GTA
Cuts at: 493 3885 493
Size: 3392 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6094
SphI G_CATG'C
Cuts at: 1132 3452 1132
Size: 2320 3774
SspI AAT'ATT
Cuts at: 5 52 4018 5
Size: 47 3966 2081
Fragments arranged by size:
3966 2081 47
StuI AGG'CCT
Cuts at: 1517 2415 3339 1517
Size: 898 924 4272
Fragments arranged by size:
4272 924 898
StyI C'CwwG_G
Cuts at: 513 1065 2004 2637 3097 3533 513
Size: 552 939 633 460 436 3074
Fragments arranged by size:
3074 939 633 552 460 436
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4121 4138 4291 4476 4921 325
Size: 1255 2541 17 153 185 445 1498
Fragments arranged by size:
2541 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2183 4340 6052
Size: 274 80 33 51 1443 206 2157 1712
Cuts at: 6052 96
Size: 138
Fragments arranged by size:
2157 1712 1443 274 206 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6054 6086 6054
Size: 32 6062
Tth111I GACn'n_nGTC
Cuts at: 1316 2767 1316
Size: 1451 4643
XbaI T'CTAG_A
Cuts at: 3430 3430
Size: 6094
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6094
XmaI C'CCGG_G
Cuts at: 1870 2484 3419 1870
Size: 614 935 4545
Fragments arranged by size:
4545 935 614
XmnI GAAnn'nnTTC
Cuts at: 4223 4223
Size: 6094
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3902 276
Size: 53 83 186 936 2368 2468
Fragments arranged by size:
2468 2368 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SrfI SwaI XcmI