Restriction map for
pFN21AB9433
(Circular) (Six-base) MAPSORT of: pFN21AB9433.seq Check: 8808 from: 1 to: 5521
pFN21AB9433 5521 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3331 278
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2864 1937
Size: 927 4594
Acc65I G'GTAC_C
Cuts at: 2842 2842
Size: 5521
AclI AA'CG_TT
Cuts at: 1563 3648 4021 1563
Size: 2085 373 3063
Fragments arranged by size:
3063 2085 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2447 2629 3584 4968 5236 1632
Size: 815 182 955 1384 268 1917
Fragments arranged by size:
1917 1384 955 815 268 182
AfeI AGC'GCT
Cuts at: 2231 2231
Size: 5521
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5324
AflIII A'CryG_T
Cuts at: 1203 2524 4420 5397 1203
Size: 1321 1896 977 1327
Fragments arranged by size:
1896 1327 1321 977
AgeI A'CCGG_T
Cuts at: 4352 4352
Size: 5521
AhdI GACnn_n'nnGTC
Cuts at: 2062 2382 4250 2062
Size: 320 1868 3333
Fragments arranged by size:
3333 1868 320
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5521
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5521
AlwNI CAG_nnn'CTG
Cuts at: 2494 2536 2707 3264 4836 2494
Size: 42 171 557 1572 3179
Fragments arranged by size:
3179 1572 557 171 42
ApaLI G'TGCA_C
Cuts at: 3578 4734 3578
Size: 1156 4365
ApoI r'AATT_y
Cuts at: 1493 2830 3056 3208 5317 1493
Size: 1337 226 152 2109 1697
Fragments arranged by size:
2109 1697 1337 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4075 160
Size: 872 3043 1606
Fragments arranged by size:
3043 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
AvaI C'yCGr_G
Cuts at: 1870 1943 2032 2123 2846 1870
Size: 73 89 91 723 4545
Fragments arranged by size:
4545 723 91 89 73
BamHI G'GATC_C
Cuts at: 2851 3218 2851
Size: 367 5154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2112 2842 4297 618
Size: 325 217 627 325 730 1455 1842
Fragments arranged by size:
1842 1455 730 627 325 325 217
BanII G_rGCy'C
Cuts at: 1391 2840 1391
Size: 1449 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2799 928
Size: 971 900 3650
Fragments arranged by size:
3650 971 900
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3712 3746 1903
Size: 34 1775 34 3678
Fragments arranged by size:
3678 1775 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3413 4623 3413
Size: 1210 4311
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5521
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2798 4132 136
Size: 107 122 71 631 605 1126 1334 1525
Fragments arranged by size:
1525 1334 1126 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5516 5516
Size: 5521
BlpI GC'TnA_GC
Cuts at: 2129 2600 2609 2938 2129
Size: 471 9 329 4712
Fragments arranged by size:
4712 471 329 9
Bme1580I G_kGCm'C
Cuts at: 1790 3582 4738 1790
Size: 1792 1156 2573
Fragments arranged by size:
2573 1792 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5521
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3252 4210 442
Size: 416 53 83 399 24 1835 958 1753
Fragments arranged by size:
1835 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2740 2759 2797 2740
Size: 19 38 5464
Fragments arranged by size:
5464 38 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2474 4181 1239
Size: 1235 1707 2579
Fragments arranged by size:
2579 1707 1235
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3646 4511 4809 3646
Size: 865 298 4358
Fragments arranged by size:
4358 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4184 882
Size: 617 2685 2219
Fragments arranged by size:
2685 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2443 3312 493
Size: 711 1239 869 2702
Fragments arranged by size:
2702 1239 869 711
BsaBI GATnn'nnATC
Cuts at: 5515 5515
Size: 5521
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2801 3328 3710 4345
Size: 53 83 186 936 1268 527 382 635
Cuts at: 4345 275
Size: 1451
Fragments arranged by size:
1451 1268 936 635 527 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2104 3954 4352 4626 4773 5184 2104
Size: 1850 398 274 147 411 2441
Fragments arranged by size:
2441 1850 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5296
Fragments arranged by size:
5296 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 2140 2346 2558 2748 1186
Size: 838 3 113 206 212 190 3959
Fragments arranged by size:
3959 838 212 206 190 113 3
BseYI C'CCAG_C
Cuts at: 2515 4724 5220 2515
Size: 2209 496 2816
Fragments arranged by size:
2816 2209 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4948
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2406 2971 3732 4760 1540
Size: 275 186 405 565 761 1028 2301
Fragments arranged by size:
2301 1028 761 565 405 275 186
BsiHKAI G_wGCw'C
Cuts at: 3582 3667 4738 3582
Size: 85 1071 4365
Fragments arranged by size:
4365 1071 85
BsmI GAATG_Cn'
Cuts at: 3032 3125 3032
Size: 93 5428
BsmBI CGTCTCn'nnnn_
Cuts at: 2036 2036
Size: 5521
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2840 3582 3667 4738 1391
Size: 399 1050 742 85 1071 2174
Fragments arranged by size:
2174 1071 1050 742 399 85
BspEI T'CCGG_A
Cuts at: 5184 5184
Size: 5521
BspHI T'CATG_A
Cuts at: 3410 5140 3410
Size: 1730 3791
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2250 2715 2876 844
Size: 1406 465 161 3489
Fragments arranged by size:
3489 1406 465 161
BsrBI CCG'CTC
Cuts at: 1394 1823 2613 3408 1394
Size: 429 790 795 3507
Fragments arranged by size:
3507 795 790 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4016 4190 65
Size: 1183 2768 174 1396
Fragments arranged by size:
2768 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2604 2660 2683 4165 4352 1700
Size: 110 794 56 23 1482 187 2869
Fragments arranged by size:
2869 1482 794 187 110 56 23
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5521
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5521
BssSI C'ACGA_G
Cuts at: 3581 4593 3581
Size: 1012 4509
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2707 794
Size: 1913 3608
BstBI TT'CG_AA
Cuts at: 4325 4325
Size: 5521
BstEII G'GTnAC_C
Cuts at: 1184 2663 1184
Size: 1479 4042
BstYI r'GATC_y
Cuts at: 1972 2083 2851 3218 3603 3620 5072 5158 5170
Size: 111 768 367 385 17 1452 86 12
Cuts at: 5170 5516 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 768 385 367 346 111 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2211 513
Size: 552 939 207 3823
Fragments arranged by size:
3823 939 552 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4782
Fragments arranged by size:
4782 683 56
BtsI GCAGTG_nn'
Cuts at: 2293 2534 3037 3831 3851 2293
Size: 241 503 794 20 3963
Fragments arranged by size:
3963 794 503 241 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5521
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5486
DraI TTT'AAA
Cuts at: 2825 2825
Size: 5521
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4528 809
Size: 507 3212 1802
Fragments arranged by size:
3212 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2403 2968 3857
Size: 54 1425 50 275 18 573 565 889
Cuts at: 3857 5486 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 573 565 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2403 2968 1537
Size: 275 591 565 4090
Fragments arranged by size:
4090 591 565 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2034 2648 2709 3451 1353
Size: 681 614 61 742 3423
Fragments arranged by size:
3423 742 681 614 61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2096 2175 2329 4108 4482 4628
Size: 1064 138 507 79 154 1779 374 146
Cuts at: 4628 387
Size: 1280
Fragments arranged by size:
1779 1280 1064 507 374 154 146 138
79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2447 2474 2629 3584 4181 4968 5236
Size: 393 815 27 155 955 597 787 268
Cuts at: 5236 1239
Size: 1524
Fragments arranged by size:
1524 955 815 787 597 393 268 155
27
EcoO109I rG'GnC_Cy
Cuts at: 2141 2704 2141
Size: 563 4958
EcoRI G'AATT_C
Cuts at: 2830 3208 2830
Size: 378 5143
FspI TGC'GCA
Cuts at: 4027 4027
Size: 5521
HaeII r_GCGC'y
Cuts at: 1678 2233 2304 4668 1678
Size: 555 71 2364 2531
Fragments arranged by size:
2531 2364 555 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2048 2080 2368 2400 2750
Size: 32 135 32 476 32 288 32 350
Cuts at: 2750 2782 4162 4194 4236 4268 1373
Size: 32 1380 32 42 32 2626
Fragments arranged by size:
2626 1380 476 350 288 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2865 3111 669
Size: 988 281 927 246 3079
Fragments arranged by size:
3079 988 927 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5521
HpaI GTT'AAC
Cuts at: 3111 3111
Size: 5521
KpnI G_GTAC'C
Cuts at: 2846 2846
Size: 5521
MfeI C'AATT_G
Cuts at: 3120 3120
Size: 5521
MluI A'CGCG_T
Cuts at: 2524 5397 2524
Size: 2873 2648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4635 4819 1305
Size: 3330 184 2007
Fragments arranged by size:
3330 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3699
Fragments arranged by size:
3699 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3479 3838 3997 518
Size: 648 2313 359 159 2042
Fragments arranged by size:
2313 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2213 2352 2408 2937 3614 4762 5007 1606
Size: 607 139 56 529 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 607 529 245 139 56
NaeI GCC'GGC
Cuts at: 2606 2606
Size: 5521
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4030
Fragments arranged by size:
4030 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5521
NgoMIV G'CCGG_C
Cuts at: 2604 2604
Size: 5521
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5521
NotI GC'GGCC_GC
Cuts at: 2403 2968 2403
Size: 565 4956
NspI r_CATG'y
Cuts at: 1132 1229 2879 4424 1132
Size: 97 1650 1545 2229
Fragments arranged by size:
2229 1650 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5521
PciI A'CATG_T
Cuts at: 4420 4420
Size: 5521
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5521
PfoI T'CCnGG_A
Cuts at: 2088 2088
Size: 5521
PmeI GTTT'AAAC
Cuts at: 2825 2825
Size: 5521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3590 3622 5103 5135 3590
Size: 32 1481 32 3976
Fragments arranged by size:
3976 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2704 2704
Size: 5521
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5521
PsiI TTA'TAA
Cuts at: 3091 3091
Size: 5521
PstI C_TGCA'G
Cuts at: 830 2279 2541 2873 830
Size: 1449 262 332 3478
Fragments arranged by size:
3478 1449 332 262
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5521
SacII CC_GC'GG
Cuts at: 2214 2214
Size: 5521
SalI G'TCGA_C
Cuts at: 1936 2863 1936
Size: 927 4594
SapI GCTCTTCn'nnn_
Cuts at: 2648 2648
Size: 5521
SbfI CC_TGCA'GG
Cuts at: 2873 2873
Size: 5521
ScaI AGT'ACT
Cuts at: 3769 5481 3769
Size: 1712 3809
SfcI C'TryA_G
Cuts at: 826 1045 2275 2537 2869 4004 4685 4876 826
Size: 219 1230 262 332 1135 681 191 1471
Fragments arranged by size:
1471 1230 1135 681 332 262 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5521
SgrAI Cr'CCGG_yG
Cuts at: 1700 2660 1700
Size: 960 4561
SmaI CCC'GGG
Cuts at: 1872 2034 2125 2848 1872
Size: 162 91 723 4545
Fragments arranged by size:
4545 723 162 91
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3625 4526 4788 820
Size: 197 926 1682 901 262 1553
Fragments arranged by size:
1682 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3312 493
Size: 2819 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5521
SphI G_CATG'C
Cuts at: 1132 2879 1132
Size: 1747 3774
SspI AAT'ATT
Cuts at: 5 52 3445 5
Size: 47 3393 2081
Fragments arranged by size:
3393 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2960 513
Size: 552 939 956 3074
Fragments arranged by size:
3074 956 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3548 3565 3718 3903 4348 325
Size: 1255 1968 17 153 185 445 1498
Fragments arranged by size:
1968 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3767 5479 96
Size: 274 80 33 51 1443 1790 1712 138
Fragments arranged by size:
1790 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5481 5513 5481
Size: 32 5489
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5521
XbaI T'CTAG_A
Cuts at: 2857 2857
Size: 5521
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5521
XmaI C'CCGG_G
Cuts at: 1870 2032 2123 2846 1870
Size: 162 91 723 4545
Fragments arranged by size:
4545 723 162 91
XmnI GAAnn'nnTTC
Cuts at: 3650 3650
Size: 5521
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3329 276
Size: 53 83 186 936 1795 2468
Fragments arranged by size:
2468 1795 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacII SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII
SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI