Restriction map for
pFN21AB9428
(Circular) (Six-base) MAPSORT of: pFN21AB9428.seq Check: 5520 from: 1 to: 6259
pFN21AB9428 6259 bp
With 182 enzymes: SgfI *
July 6, 2012 17:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2616 4069 278
Size: 53 83 186 936 1080 1453 2468
Fragments arranged by size:
2468 1453 1080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2616 3602 1937
Size: 679 986 4594
Fragments arranged by size:
4594 986 679
Acc65I G'GTAC_C
Cuts at: 3580 3580
Size: 6259
AclI AA'CG_TT
Cuts at: 1563 4386 4759 1563
Size: 2823 373 3063
Fragments arranged by size:
3063 2823 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2798 3153 3534 4322 5706 5974 1632
Size: 1166 355 381 788 1384 268 1917
Fragments arranged by size:
1917 1384 1166 788 381 355 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6062
AflIII A'CryG_T
Cuts at: 1203 5158 6135 1203
Size: 3955 977 1327
Fragments arranged by size:
3955 1327 977
AgeI A'CCGG_T
Cuts at: 5090 5090
Size: 6259
AhdI GACnn_n'nnGTC
Cuts at: 4988 4988
Size: 6259
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3241 3273 3241
Size: 32 6227
AlwNI CAG_nnn'CTG
Cuts at: 4002 5574 4002
Size: 1572 4687
ApaLI G'TGCA_C
Cuts at: 4316 5472 4316
Size: 1156 5103
ApoI r'AATT_y
Cuts at: 1493 2213 2288 3568 3794 3946 6055 1493
Size: 720 75 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 720 226 152 75
AseI AT'TA_AT
Cuts at: 160 1032 4813 160
Size: 872 3781 1606
Fragments arranged by size:
3781 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
AvaI C'yCGr_G
Cuts at: 1870 1943 3100 3267 3584 1870
Size: 73 1157 167 317 4545
Fragments arranged by size:
4545 1157 317 167 73
BamHI G'GATC_C
Cuts at: 2628 3589 3956 2628
Size: 961 367 4931
Fragments arranged by size:
4931 961 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2033 2546 3442 3580 5035
Size: 325 217 627 246 513 896 138 1455
Cuts at: 5035 618
Size: 1842
Fragments arranged by size:
1842 1455 896 627 513 325 246 217
138
BanII G_rGCy'C
Cuts at: 1391 2082 2814 3578 1391
Size: 691 732 764 4072
Fragments arranged by size:
4072 764 732 691
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2854 928
Size: 971 955 4333
Fragments arranged by size:
4333 971 955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4450 4484 1903
Size: 34 2513 34 3678
Fragments arranged by size:
3678 2513 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4151 5361 4151
Size: 1210 5049
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6259
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4870 136
Size: 107 122 71 631 605 3198 1525
Fragments arranged by size:
3198 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6254 6254
Size: 6259
BlpI GC'TnA_GC
Cuts at: 2733 3676 2733
Size: 943 5316
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2336 2549 3127 4320 5476 1790
Size: 248 298 213 578 1193 1156 2573
Fragments arranged by size:
2573 1193 1156 578 298 248 213
BmgBI CAC'GTC
Cuts at: 1318 2470 1318
Size: 1152 5107
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2263 2323 3990
Size: 416 53 83 399 24 846 60 1667
Cuts at: 3990 4948 442
Size: 958 1753
Fragments arranged by size:
1753 1667 958 846 416 399 83 60
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6259
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 6259
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2821 3183 3541 4919 1239
Size: 848 734 362 358 1378 2579
Fragments arranged by size:
2579 1378 848 734 362 358
Bpu10I CC'TnA_GC
Cuts at: 2927 2927
Size: 6259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2417 4384 5249 5547 2417
Size: 1967 865 298 3129
Fragments arranged by size:
3129 1967 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3197 4922 882
Size: 617 1698 1725 2219
Fragments arranged by size:
2219 1725 1698 617
BsaAI yAC'GTr
Cuts at: 493 1204 4050 493
Size: 711 2846 2702
Fragments arranged by size:
2846 2702 711
BsaBI GATnn'nnATC
Cuts at: 6253 6253
Size: 6259
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2613 4066 4448 5083
Size: 53 83 186 936 1080 1453 382 635
Cuts at: 5083 275
Size: 1451
Fragments arranged by size:
1453 1451 1080 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2589 4692 5090 5364 5511 5922 2589
Size: 2103 398 274 147 411 2926
Fragments arranged by size:
2926 2103 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2810 2840 1178
Size: 30 165 30 1407 30 4597
Fragments arranged by size:
4597 1407 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3083 3130 3146 1186
Size: 1897 47 16 4299
Fragments arranged by size:
4299 1897 47 16
BseYI C'CCAG_C
Cuts at: 2027 3077 3230 5462 5958 2027
Size: 1050 153 2232 496 2328
Fragments arranged by size:
2328 2232 1050 496 153
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2405 2450 1163
Size: 573 645 24 45 4972
Fragments arranged by size:
4972 645 573 45 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3347 3709 4470 5498 1540
Size: 275 186 1346 362 761 1028 2301
Fragments arranged by size:
2301 1346 1028 761 362 275 186
BsiHKAI G_wGCw'C
Cuts at: 2082 2418 4320 4405 5476 2082
Size: 336 1902 85 1071 2865
Fragments arranged by size:
2865 1902 1071 336 85
BsmI GAATG_Cn'
Cuts at: 2843 2893 3770 3863 2843
Size: 50 877 93 5239
Fragments arranged by size:
5239 877 93 50
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2082 2336 2418 2549 2814 3127
Size: 399 248 44 254 82 131 265 313
Cuts at: 3127 3578 4320 4405 5476 1391
Size: 451 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 451 399 313 265 254
248 131 85 82 44
BspEI T'CCGG_A
Cuts at: 5922 5922
Size: 6259
BspHI T'CATG_A
Cuts at: 4148 5878 4148
Size: 1730 4529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2403 3127 3614 844
Size: 1559 724 487 3489
Fragments arranged by size:
3489 1559 724 487
BsrBI CCG'CTC
Cuts at: 1394 1823 2350 3349 4146 1394
Size: 429 527 999 797 3507
Fragments arranged by size:
3507 999 797 527 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4754 4928 65
Size: 1183 3506 174 1396
Fragments arranged by size:
3506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2272 2718 4903 5090 1700
Size: 110 462 446 2185 187 2869
Fragments arranged by size:
2869 2185 462 446 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6259
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6259
BssSI C'ACGA_G
Cuts at: 2687 4319 5331 2687
Size: 1632 1012 3615
Fragments arranged by size:
3615 1632 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2648 794
Size: 1854 4405
BstBI TT'CG_AA
Cuts at: 5063 5063
Size: 6259
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6259
BstXI CCAn_nnnn'nTGG
Cuts at: 3150 3537 3150
Size: 387 5872
BstYI r'GATC_y
Cuts at: 1972 2263 2628 3508 3589 3956 4341 4358 5810
Size: 291 365 880 81 367 385 17 1452
Cuts at: 5810 5896 5908 6254 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 880 385 367 365 346 291
86 81 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2193 2901 513
Size: 552 1128 708 3871
Fragments arranged by size:
3871 1128 708 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5520
Fragments arranged by size:
5520 683 56
BtsI GCAGTG_nn'
Cuts at: 2649 2706 2812 3775 4569 4589 2649
Size: 57 106 963 794 20 4319
Fragments arranged by size:
4319 963 794 106 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6259
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6224
DraI TTT'AAA
Cuts at: 3563 3563
Size: 6259
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5266 809
Size: 507 3950 1802
Fragments arranged by size:
3950 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2345 3706 4595
Size: 54 1425 50 275 18 515 1361 889
Cuts at: 4595 6224 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1361 889 515 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3706 1537
Size: 275 1894 4090
Fragments arranged by size:
4090 1894 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2650 4189 1353
Size: 1297 1539 3423
Fragments arranged by size:
3423 1539 1297
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4846 5220 5366 387
Size: 1064 138 3257 374 146 1280
Fragments arranged by size:
3257 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2080 2080
Size: 6259
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2798 2821 3153 3183 3534 3541
Size: 393 455 711 23 332 30 351 7
Cuts at: 3541 4322 4919 5706 5974 1239
Size: 781 597 787 268 1524
Fragments arranged by size:
1524 787 781 711 597 455 393 351
332 268 30 23 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3523 3523
Size: 6259
EcoO109I rG'GnC_Cy
Cuts at: 2428 3315 3500 2428
Size: 887 185 5187
Fragments arranged by size:
5187 887 185
EcoRI G'AATT_C
Cuts at: 2213 3568 3946 2213
Size: 1355 378 4526
Fragments arranged by size:
4526 1355 378
EcoRV GAT'ATC
Cuts at: 2175 2175
Size: 6259
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2396 2396
Size: 6259
FspI TGC'GCA
Cuts at: 4765 4765
Size: 6259
HaeII r_GCGC'y
Cuts at: 1678 5406 1678
Size: 3728 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2810 2842 2903 2935 2937
Size: 32 135 32 1238 32 61 32 2
Cuts at: 2937 2969 4900 4932 4974 5006 1373
Size: 32 1931 32 42 32 2626
Fragments arranged by size:
2626 1931 1238 135 61 42 32 32
32 32 32 32 32 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2581 3355 3603 3849 669
Size: 988 281 643 774 248 246 3079
Fragments arranged by size:
3079 988 774 643 281 248 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6259
HpaI GTT'AAC
Cuts at: 2581 3355 3849 2581
Size: 774 494 4991
Fragments arranged by size:
4991 774 494
KpnI G_GTAC'C
Cuts at: 3584 3584
Size: 6259
MfeI C'AATT_G
Cuts at: 3858 3858
Size: 6259
MluI A'CGCG_T
Cuts at: 6135 6135
Size: 6259
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 3364 5373 5557 1305
Size: 1161 898 2009 184 2007
Fragments arranged by size:
2009 2007 1161 898 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4437
Fragments arranged by size:
4437 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4217 4576 4735 518
Size: 648 3051 359 159 2042
Fragments arranged by size:
3051 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2031 3675 4352 5500 5745 1606
Size: 425 1644 677 1148 245 2120
Fragments arranged by size:
2120 1644 1148 677 425 245
NaeI GCC'GGC
Cuts at: 2274 2274
Size: 6259
NcoI C'CATG_G
Cuts at: 513 1065 2193 513
Size: 552 1128 4579
Fragments arranged by size:
4579 1128 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6259
NgoMIV G'CCGG_C
Cuts at: 2272 2272
Size: 6259
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6259
NotI GC'GGCC_GC
Cuts at: 3706 3706
Size: 6259
NspI r_CATG'y
Cuts at: 1132 1229 3617 5162 1132
Size: 97 2388 1545 2229
Fragments arranged by size:
2388 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2551 1348
Size: 1203 5056
PciI A'CATG_T
Cuts at: 5158 5158
Size: 6259
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2872 3260 1108
Size: 1764 388 4107
Fragments arranged by size:
4107 1764 388
PfoI T'CCnGG_A
Cuts at: 2832 3259 2832
Size: 427 5832
PmeI GTTT'AAAC
Cuts at: 3563 3563
Size: 6259
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2670 2702 4328 4360 5841 5873 2670
Size: 32 1626 32 1481 32 3056
Fragments arranged by size:
3056 1626 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2428 3315 2428
Size: 887 5372
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6259
PsiI TTA'TAA
Cuts at: 3829 3829
Size: 6259
PstI C_TGCA'G
Cuts at: 830 2325 3314 3611 830
Size: 1495 989 297 3478
Fragments arranged by size:
3478 1495 989 297
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6259
SacI G_AGCT'C
Cuts at: 2082 2082
Size: 6259
SalI G'TCGA_C
Cuts at: 1936 3601 1936
Size: 1665 4594
SbfI CC_TGCA'GG
Cuts at: 3314 3611 3314
Size: 297 5962
ScaI AGT'ACT
Cuts at: 4507 6219 4507
Size: 1712 4547
SfcI C'TryA_G
Cuts at: 826 1045 2321 2617 3310 3607 4742 5423 5614
Size: 219 1276 296 693 297 1135 681 191
Cuts at: 5614 826
Size: 1471
Fragments arranged by size:
1471 1276 1135 693 681 297 296 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6259
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6259
SmaI CCC'GGG
Cuts at: 1872 3586 1872
Size: 1714 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2432 4363 5264 5526 820
Size: 197 926 489 1931 901 262 1553
Fragments arranged by size:
1931 1553 926 901 489 262 197
SnaBI TAC'GTA
Cuts at: 493 4050 493
Size: 3557 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6259
SphI G_CATG'C
Cuts at: 1132 3617 1132
Size: 2485 3774
SspI AAT'ATT
Cuts at: 5 52 4183 5
Size: 47 4131 2081
Fragments arranged by size:
4131 2081 47
StuI AGG'CCT
Cuts at: 1517 2597 1517
Size: 1080 5179
StyI C'CwwG_G
Cuts at: 513 1065 2046 2193 2814 2968 3698 513
Size: 552 981 147 621 154 730 3074
Fragments arranged by size:
3074 981 730 621 552 154 147
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4286 4303 4456 4641 5086 325
Size: 1255 2706 17 153 185 445 1498
Fragments arranged by size:
2706 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4505 6217 96
Size: 274 80 33 51 1443 2528 1712 138
Fragments arranged by size:
2528 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6219 6251 6219
Size: 32 6227
Tth111I GACn'n_nGTC
Cuts at: 1316 2527 1316
Size: 1211 5048
XbaI T'CTAG_A
Cuts at: 3064 3595 3064
Size: 531 5728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2062 2200 2275 2449 3436 2062
Size: 138 75 174 987 4885
Fragments arranged by size:
4885 987 174 138 75
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6259
XmaI C'CCGG_G
Cuts at: 1870 3584 1870
Size: 1714 4545
XmnI GAAnn'nnTTC
Cuts at: 4388 4388
Size: 6259
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2614 4067 276
Size: 53 83 186 936 1080 1453 2468
Fragments arranged by size:
2468 1453 1080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI