Restriction map for
pFN21AB9419
(Circular) (Six-base) MAPSORT of: pFN21AB9419.seq Check: 2192 from: 1 to: 6334
pFN21AB9419 6334 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3677 1937
Size: 1740 4594
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2124 3045 3348 4397 5781 6049 1632
Size: 492 921 303 1049 1384 268 1917
Fragments arranged by size:
1917 1384 1049 921 492 303 268
AflII C'TTAA_G
Cuts at: 820 1017 3083 820
Size: 197 2066 4071
Fragments arranged by size:
4071 2066 197
AflIII A'CryG_T
Cuts at: 1203 5233 6210 1203
Size: 4030 977 1327
Fragments arranged by size:
4030 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 2793 5063 2793
Size: 2270 4064
AleI CACnn'nnGTG
Cuts at: 1166 2117 1166
Size: 951 5383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AlwNI CAG_nnn'CTG
Cuts at: 2075 3326 4077 5649 2075
Size: 1251 751 1572 2760
Fragments arranged by size:
2760 1572 1251 751
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 3050 3302 3381 3628 3643 3869 4021 6130
Size: 1557 252 79 247 15 226 152 2109
Cuts at: 6130 1493
Size: 1697
Fragments arranged by size:
2109 1697 1557 252 247 226 152 79
15
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2431 2823 3659 1870
Size: 73 488 392 836 4545
Fragments arranged by size:
4545 836 488 392 73
BamHI G'GATC_C
Cuts at: 2195 3664 4031 2195
Size: 1469 367 4498
Fragments arranged by size:
4498 1469 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2245 3655 5110 618
Size: 325 217 627 458 1410 1455 1842
Fragments arranged by size:
1842 1455 1410 627 458 325 217
BanII G_rGCy'C
Cuts at: 1391 3653 1391
Size: 2262 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2334 3276 3591 928
Size: 971 435 942 315 3671
Fragments arranged by size:
3671 971 942 435 315
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4525 4559 1903
Size: 34 2588 34 3678
Fragments arranged by size:
3678 2588 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2660 3461 4226 5436 2660
Size: 801 765 1210 3558
Fragments arranged by size:
3558 1210 801 765
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3489 4945 136
Size: 107 122 71 631 605 1817 1456 1525
Fragments arranged by size:
1817 1525 1456 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3190 6329 3190
Size: 3139 3195
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 3485 4395 5551 1790
Size: 1695 910 1156 2573
Fragments arranged by size:
2573 1695 1156 910
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4065 5023 442
Size: 416 53 83 399 24 2648 958 1753
Fragments arranged by size:
2648 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 4994 1239
Size: 813 2942 2579
Fragments arranged by size:
2942 2579 813
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2775 4459 5324 5622 2775
Size: 1684 865 298 3487
Fragments arranged by size:
3487 1684 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4997 882
Size: 617 3498 2219
Fragments arranged by size:
3498 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4125 493
Size: 711 2921 2702
Fragments arranged by size:
2921 2702 711
BsaBI GATnn'nnATC
Cuts at: 6328 6328
Size: 6334
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4141 4523 5158 275
Size: 53 83 186 936 2608 382 635 1451
Fragments arranged by size:
2608 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6109
Fragments arranged by size:
6109 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6334
BseYI C'CCAG_C
Cuts at: 5537 6033 5537
Size: 496 5838
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3318 1163
Size: 573 1582 4179
Fragments arranged by size:
4179 1582 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3784 4545 5573 1540
Size: 275 186 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2689 4395 4480 5551 2689
Size: 1706 85 1071 3472
Fragments arranged by size:
3472 1706 1071 85
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2689 3485 3653 4395 4480 5551 1391
Size: 399 899 796 168 742 85 1071 2174
Fragments arranged by size:
2174 1071 899 796 742 399 168 85
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 4223 5953 4223
Size: 1730 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3689 844
Size: 2845 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4221 1394
Size: 429 2398 3507
Fragments arranged by size:
3507 2398 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4978 5165 1700
Size: 110 3168 187 2869
Fragments arranged by size:
3168 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2807 794
Size: 2013 4321
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstXI CCAn_nnnn'nTGG
Cuts at: 3315 3315
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2195 3190 3664 4031 4416 4433 5885 5971
Size: 223 995 474 367 385 17 1452 86
Cuts at: 5971 5983 6329 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 995 474 385 367 346 223
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2200 3486 2200
Size: 1286 5048
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5595
Fragments arranged by size:
5595 683 56
BtsI GCAGTG_nn'
Cuts at: 3594 3850 4644 4664 3594
Size: 256 794 20 5264
Fragments arranged by size:
5264 794 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3781 4670 6299
Size: 54 1425 50 275 18 1951 889 1629
Cuts at: 6299 8
Size: 43
Fragments arranged by size:
1951 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3781 1537
Size: 275 1969 4090
Fragments arranged by size:
4090 1969 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4264 1353
Size: 2911 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4921 5295 5441 387
Size: 1064 138 3332 374 146 1280
Fragments arranged by size:
3332 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2124 3045 3348 4397 4994 5781
Size: 393 420 72 921 303 1049 597 787
Cuts at: 5781 6049 1239
Size: 268 1524
Fragments arranged by size:
1524 1049 921 787 597 420 393 303
268 72
EcoNI CCTnn'n_nnAGG
Cuts at: 2217 2217
Size: 6334
EcoO109I rG'GnC_Cy
Cuts at: 3538 3538
Size: 6334
EcoRI G'AATT_C
Cuts at: 3381 3643 4021 3381
Size: 262 378 5694
Fragments arranged by size:
5694 378 262
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2972 2972
Size: 6334
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 2450 5481 1678
Size: 772 3031 2531
Fragments arranged by size:
3031 2531 772
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2658 2690 2779 2811 3356
Size: 32 135 32 1086 32 89 32 545
Cuts at: 3356 3388 4975 5007 5049 5081 1373
Size: 32 1587 32 42 32 2626
Fragments arranged by size:
2626 1587 1086 545 135 89 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2757 3678 3924 669
Size: 988 281 819 921 246 3079
Fragments arranged by size:
3079 988 921 819 281 246
HindIII A'AGCT_T
Cuts at: 748 2949 3067 3110 748
Size: 2201 118 43 3972
Fragments arranged by size:
3972 2201 118 43
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2795 3509 5448 5632 1305
Size: 1490 714 1939 184 2007
Fragments arranged by size:
2007 1939 1490 714 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4512
Fragments arranged by size:
4512 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2117 2736 4292 4651 4810 518
Size: 648 951 619 1556 359 159 2042
Fragments arranged by size:
2042 1556 951 648 619 359 159
MspA1I CmG'CkG
Cuts at: 1606 3121 3750 4427 5575 5820 1606
Size: 1515 629 677 1148 245 2120
Fragments arranged by size:
2120 1515 1148 677 629 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 2735 3692 5237 1132
Size: 97 1506 957 1545 2229
Fragments arranged by size:
2229 1545 1506 957 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6334
PfoI T'CCnGG_A
Cuts at: 2527 2527
Size: 6334
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 5916 5948 4403
Size: 32 1481 32 4789
Fragments arranged by size:
4789 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3538 3538
Size: 6334
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PstI C_TGCA'G
Cuts at: 830 2455 2706 3686 830
Size: 1625 251 980 3478
Fragments arranged by size:
3478 1625 980 251
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 3121 3121
Size: 6334
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 3447 4582 6294 3447
Size: 1135 1712 3487
Fragments arranged by size:
3487 1712 1135
SexAI A'CCwGG_T
Cuts at: 2929 2929
Size: 6334
SfcI C'TryA_G
Cuts at: 826 1045 2115 2322 2409 2451 2702 3600 3682
Size: 219 1070 207 87 42 251 898 82
Cuts at: 3682 4817 5498 5689 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1070 898 681 251 219 207
191 87 82 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 3661 1872
Size: 1789 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2790 2823 3083 4438 5339 5601
Size: 197 926 847 33 260 1355 901 262
Cuts at: 5601 820
Size: 1553
Fragments arranged by size:
1553 1355 926 901 847 262 260 197
33
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 3397 152
Size: 3245 3089
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 3208 4258 5
Size: 47 3156 1050 2081
Fragments arranged by size:
3156 2081 1050 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6334
StyI C'CwwG_G
Cuts at: 513 1065 2004 3773 513
Size: 552 939 1769 3074
Fragments arranged by size:
3074 1769 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4361 4378 4531 4716 5161 325
Size: 1255 2781 17 153 185 445 1498
Fragments arranged by size:
2781 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2081 3445 4580
Size: 274 80 33 51 1443 104 1364 1135
Cuts at: 4580 6292 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1364 1135 274 138 104 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6294 6326 6294
Size: 32 6302
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XhoI C'TCGA_G
Cuts at: 1943 2823 1943
Size: 880 5454
XmaI C'CCGG_G
Cuts at: 1870 3659 1870
Size: 1789 4545
XmnI GAAnn'nnTTC
Cuts at: 2355 3567 4463 2355
Size: 1212 896 4226
Fragments arranged by size:
4226 1212 896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI