Restriction map for
pFN21AB9418
(Circular) (Six-base) MAPSORT of: pFN21AB9418.seq Check: 9389 from: 1 to: 6562
pFN21AB9418 6562 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2458 2458
Size: 6562
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4372 278
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3905 1937
Size: 1968 4594
Acc65I G'GTAC_C
Cuts at: 3883 3883
Size: 6562
AclI AA'CG_TT
Cuts at: 1563 4689 5062 1563
Size: 3126 373 3063
Fragments arranged by size:
3126 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 2409 2469 2919 3571 3651 3747 4625
Size: 600 177 60 450 652 80 96 878
Cuts at: 4625 6009 6277 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 878 652 600 450 268 177
96 80 60
AfeI AGC'GCT
Cuts at: 2855 2855
Size: 6562
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6365
AflIII A'CryG_T
Cuts at: 1203 2116 5461 6438 1203
Size: 913 3345 977 1327
Fragments arranged by size:
3345 1327 977 913
AgeI A'CCGG_T
Cuts at: 5393 5393
Size: 6562
AhdI GACnn_n'nnGTC
Cuts at: 5291 5291
Size: 6562
AleI CACnn'nnGTG
Cuts at: 1166 2420 1166
Size: 1254 5308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6562
AlwNI CAG_nnn'CTG
Cuts at: 3386 4305 5877 3386
Size: 919 1572 4071
Fragments arranged by size:
4071 1572 919
ApaI G_GGCC'C
Cuts at: 3506 3506
Size: 6562
ApaLI G'TGCA_C
Cuts at: 4619 5775 4619
Size: 1156 5406
ApoI r'AATT_y
Cuts at: 1493 2042 3049 3238 3871 4097 4249 6358 1493
Size: 549 1007 189 633 226 152 2109 1697
Fragments arranged by size:
2109 1697 1007 633 549 226 189 152
AseI AT'TA_AT
Cuts at: 160 1032 5116 160
Size: 872 4084 1606
Fragments arranged by size:
4084 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
AvaI C'yCGr_G
Cuts at: 1870 1943 3887 1870
Size: 73 1944 4545
Fragments arranged by size:
4545 1944 73
AvrII C'CTAG_G
Cuts at: 3302 3302
Size: 6562
BamHI G'GATC_C
Cuts at: 2911 3892 4259 2911
Size: 981 367 5214
Fragments arranged by size:
5214 981 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3883 5338 618
Size: 325 217 627 2096 1455 1842
Fragments arranged by size:
2096 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2175 3482 3506 3881 1391
Size: 784 1307 24 375 4072
Fragments arranged by size:
4072 1307 784 375 24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2925 928
Size: 971 1026 4565
Fragments arranged by size:
4565 1026 971
BbvCI CC'TCA_GC
Cuts at: 2310 2310
Size: 6562
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4753 4787 1903
Size: 34 2816 34 3678
Fragments arranged by size:
3678 2816 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4454 5664 4454
Size: 1210 5352
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6562
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5173 136
Size: 107 122 71 631 605 3501 1525
Fragments arranged by size:
3501 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2887 6557 2887
Size: 3670 2892
BlpI GC'TnA_GC
Cuts at: 3979 3979
Size: 6562
Bme1580I G_kGCm'C
Cuts at: 1790 3506 4623 5779 1790
Size: 1716 1117 1156 2573
Fragments arranged by size:
2573 1716 1156 1117
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6562
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2405 4293 5251
Size: 416 53 83 399 24 988 1888 958
Cuts at: 5251 442
Size: 1753
Fragments arranged by size:
1888 1753 988 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6562
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2642 5222 1239
Size: 1403 2580 2579
Fragments arranged by size:
2580 2579 1403
Bpu10I CC'TnA_GC
Cuts at: 2310 2310
Size: 6562
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3666 4687 5552 5850 3666
Size: 1021 865 298 4378
Fragments arranged by size:
4378 1021 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2783 5225 882
Size: 617 1284 2442 2219
Fragments arranged by size:
2442 2219 1284 617
BsaAI yAC'GTr
Cuts at: 493 1204 4353 493
Size: 711 3149 2702
Fragments arranged by size:
3149 2702 711
BsaBI GATnn'nnATC
Cuts at: 2910 6556 2910
Size: 3646 2916
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4369 4751 5386 275
Size: 53 83 186 936 2836 382 635 1451
Fragments arranged by size:
2836 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3153 4995 5393 5667 5814 6225 3153
Size: 1842 398 274 147 411 3490
Fragments arranged by size:
3490 1842 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3115 3145 3601 3631 1178
Size: 30 165 30 1712 30 456 30 4109
Fragments arranged by size:
4109 1712 456 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 3036 3474 3609 1186
Size: 1123 727 438 135 4139
Fragments arranged by size:
4139 1123 727 438 135
BseYI C'CCAG_C
Cuts at: 2566 2697 3144 5765 6261 2566
Size: 131 447 2621 496 2867
Fragments arranged by size:
2867 2621 496 447 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3137 3582 1163
Size: 573 1401 445 4143
Fragments arranged by size:
4143 1401 573 445
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2990 4012 4773 5801 1540
Size: 275 186 989 1022 761 1028 2301
Fragments arranged by size:
2301 1028 1022 989 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2806 4623 4708 5779 2806
Size: 1817 85 1071 3589
Fragments arranged by size:
3589 1817 1071 85
BsmI GAATG_Cn'
Cuts at: 4073 4166 4073
Size: 93 6469
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2175 2806 3482 3506 3881 4623 4708
Size: 399 385 631 676 24 375 742 85
Cuts at: 4708 5779 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 676 631 399 385 375
85 24
BspEI T'CCGG_A
Cuts at: 3153 6225 3153
Size: 3072 3490
BspHI T'CATG_A
Cuts at: 2272 4451 6181 2272
Size: 2179 1730 2653
Fragments arranged by size:
2653 2179 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2458 3371 3511 3917 844
Size: 1614 913 140 406 3489
Fragments arranged by size:
3489 1614 913 406 140
BsrBI CCG'CTC
Cuts at: 1394 1823 4449 1394
Size: 429 2626 3507
Fragments arranged by size:
3507 2626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3541 3559 5057 5231 65
Size: 1183 2293 18 1498 174 1396
Fragments arranged by size:
2293 1498 1396 1183 174 18
BsrFI r'CCGG_y
Cuts at: 1700 1810 2150 2985 5206 5393 1700
Size: 110 340 835 2221 187 2869
Fragments arranged by size:
2869 2221 835 340 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6562
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6562
BssSI C'ACGA_G
Cuts at: 4622 5634 4622
Size: 1012 5550
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6562
BstBI TT'CG_AA
Cuts at: 5366 5366
Size: 6562
BstEII G'GTnAC_C
Cuts at: 1184 3607 1184
Size: 2423 4139
BstYI r'GATC_y
Cuts at: 1972 2887 2911 2938 3073 3370 3389 3892 4259
Size: 915 24 27 135 297 19 503 367
Cuts at: 4259 4644 4661 6113 6199 6211 6557 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 915 503 385 367 346 297
135 86 27 24 19 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2793 513
Size: 552 939 789 4282
Fragments arranged by size:
4282 939 789 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5823
Fragments arranged by size:
5823 683 56
BtsI GCAGTG_nn'
Cuts at: 2651 2745 4078 4872 4892 2651
Size: 94 1333 794 20 4321
Fragments arranged by size:
4321 1333 794 94 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6562
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6527
DraI TTT'AAA
Cuts at: 2719 2872 3866 2719
Size: 153 994 5415
Fragments arranged by size:
5415 994 153
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5569 809
Size: 507 4253 1802
Fragments arranged by size:
4253 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4009 4898 6527
Size: 54 1425 50 275 18 2179 889 1629
Cuts at: 6527 8
Size: 43
Fragments arranged by size:
2179 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4009 1537
Size: 275 2197 4090
Fragments arranged by size:
4090 2197 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 2794 3155 4492 1353
Size: 810 631 361 1337 3423
Fragments arranged by size:
3423 1337 810 631 361
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5149 5523 5669 387
Size: 1064 138 3560 374 146 1280
Fragments arranged by size:
3560 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 2409 2469 2642 2919 3571 3651
Size: 393 600 177 60 173 277 652 80
Cuts at: 3651 3747 4625 5222 6009 6277 1239
Size: 96 878 597 787 268 1524
Fragments arranged by size:
1524 878 787 652 600 597 393 277
268 177 173 96 80 60
EcoO109I rG'GnC_Cy
Cuts at: 3503 3523 3503
Size: 20 6542
EcoRI G'AATT_C
Cuts at: 3049 3871 4249 3049
Size: 822 378 5362
Fragments arranged by size:
5362 822 378
FspI TGC'GCA
Cuts at: 5068 5068
Size: 6562
HaeII r_GCGC'y
Cuts at: 1678 2857 5709 1678
Size: 1179 2852 2531
Fragments arranged by size:
2852 2531 1179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3601 3633 5203 5235 5277
Size: 32 135 32 2029 32 1570 32 42
Cuts at: 5277 5309 1373
Size: 32 2626
Fragments arranged by size:
2626 2029 1570 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3906 4152 669
Size: 988 281 1968 246 3079
Fragments arranged by size:
3079 1968 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2262 748
Size: 1514 5048
HpaI GTT'AAC
Cuts at: 4152 4152
Size: 6562
KpnI G_GTAC'C
Cuts at: 3887 3887
Size: 6562
MfeI C'AATT_G
Cuts at: 2289 3492 4161 2289
Size: 1203 669 4690
Fragments arranged by size:
4690 1203 669
MluI A'CGCG_T
Cuts at: 6438 6438
Size: 6562
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 3299 5676 5860 1305
Size: 739 1255 2377 184 2007
Fragments arranged by size:
2377 2007 1255 739 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4740
Fragments arranged by size:
4740 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2420 4520 4879 5038 518
Size: 648 1254 2100 359 159 2042
Fragments arranged by size:
2100 2042 1254 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3634 3978 4655 5803 6048 1606
Size: 2028 344 677 1148 245 2120
Fragments arranged by size:
2120 2028 1148 677 344 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2793 513
Size: 552 939 789 4282
Fragments arranged by size:
4282 939 789 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6562
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6562
NotI GC'GGCC_GC
Cuts at: 4009 4009
Size: 6562
NspI r_CATG'y
Cuts at: 1132 1229 2120 3920 5465 1132
Size: 97 891 1800 1545 2229
Fragments arranged by size:
2229 1800 1545 891 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6562
PciI A'CATG_T
Cuts at: 2116 5461 2116
Size: 3345 3217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2751 1108
Size: 1643 4919
PfoI T'CCnGG_A
Cuts at: 2658 2658
Size: 6562
PmeI GTTT'AAAC
Cuts at: 3866 3866
Size: 6562
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4631 4663 6144 6176 4631
Size: 32 1481 32 5017
Fragments arranged by size:
5017 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3523 3523
Size: 6562
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6562
PsiI TTA'TAA
Cuts at: 4132 4132
Size: 6562
PspOMI G'GGCC_C
Cuts at: 3502 3502
Size: 6562
PstI C_TGCA'G
Cuts at: 830 3465 3914 830
Size: 2635 449 3478
Fragments arranged by size:
3478 2635 449
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6562
PvuII CAG'CTG
Cuts at: 3634 3634
Size: 6562
SalI G'TCGA_C
Cuts at: 1936 3904 1936
Size: 1968 4594
SapI GCTCTTCn'nnn_
Cuts at: 2163 2794 3155 2163
Size: 631 361 5570
Fragments arranged by size:
5570 631 361
SbfI CC_TGCA'GG
Cuts at: 3914 3914
Size: 6562
ScaI AGT'ACT
Cuts at: 3441 4810 6522 3441
Size: 1369 1712 3481
Fragments arranged by size:
3481 1712 1369
SfcI C'TryA_G
Cuts at: 826 1045 3030 3461 3910 5045 5726 5917 826
Size: 219 1985 431 449 1135 681 191 1471
Fragments arranged by size:
1985 1471 1135 681 449 431 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6562
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6562
SmaI CCC'GGG
Cuts at: 1872 3889 1872
Size: 2017 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3681 4666 5567 5829 820
Size: 197 926 1738 985 901 262 1553
Fragments arranged by size:
1738 1553 985 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4353 493
Size: 3860 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6562
SphI G_CATG'C
Cuts at: 1132 3920 1132
Size: 2788 3774
SspI AAT'ATT
Cuts at: 5 52 4486 5
Size: 47 4434 2081
Fragments arranged by size:
4434 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6562
StyI C'CwwG_G
Cuts at: 513 1065 2004 2793 3302 4001 513
Size: 552 939 789 509 699 3074
Fragments arranged by size:
3074 939 789 699 552 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 3660 4589 4606 4759 4944 5389
Size: 1255 852 1228 929 17 153 185 445
Cuts at: 5389 325
Size: 1498
Fragments arranged by size:
1498 1255 1228 929 852 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3169 3178 3439
Size: 274 80 33 51 1443 1192 9 261
Cuts at: 3439 4808 6520 96
Size: 1369 1712 138
Fragments arranged by size:
1712 1443 1369 1192 274 261 138 80
51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3115 3147 6522 6554 3115
Size: 32 3375 32 3123
Fragments arranged by size:
3375 3123 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6562
XbaI T'CTAG_A
Cuts at: 3898 3898
Size: 6562
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3076 3076
Size: 6562
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6562
XmaI C'CCGG_G
Cuts at: 1870 3887 1870
Size: 2017 4545
XmnI GAAnn'nnTTC
Cuts at: 2118 4691 2118
Size: 2573 3989
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4370 276
Size: 53 83 186 936 2836 2468
Fragments arranged by size:
2836 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI